[Objectives] This study was conducted to develop environmentally-friendly effective vase solutions for cut rose flowers convenient for ordinary household use. [Methods] The vase life, flowering stage, flower diameter ...[Objectives] This study was conducted to develop environmentally-friendly effective vase solutions for cut rose flowers convenient for ordinary household use. [Methods] The vase life, flowering stage, flower diameter variation, petal water content, and soluble protein and malondialdehyde contents were determined on cut rose flowers regularly during the vase period, to study the fresh-keeping effects of the vase solutions prepared from such three components as sugar, aspirin and Vc according to L9(33) orthogonal table design on cut rose flowers during the vase period. [Results] 20 g/L white granulated sugar+460 mg/L aspirin+50 mg/L Vc was the best combination in this study. [Conclusions] This study provides a reference for the research on the fresh-keeping vase solutions of household cut rose flowers.展开更多
Rose has emerged as a model ornamental plant for studies of flower development, senescence, and morphology, as well as the metabolism of floral fragrances and colors.Virus-induced gene silencing(VIGS) has long been us...Rose has emerged as a model ornamental plant for studies of flower development, senescence, and morphology, as well as the metabolism of floral fragrances and colors.Virus-induced gene silencing(VIGS) has long been used in functional genomics studies of rose by vacuum infiltration of cuttings or seedlings with an Agrobacterium suspension carrying TRV-derived vectors. However, VIGS in rose flowers remains a challenge because of its low efficiency and long time to establish silencing. Here we present a novel and rapid VIGS method that can be used to analyze gene function in rose,called ‘graft-accelerated VIGS’, where axil ary sprouts are cut from the rose plant and vacuum infiltrated with Agrobacterium. The inoculated scions are then grafted back onto the plants to flower and silencing phenotypes can be observed within 5 weeks, post-infiltration. Using this new method, we successfully silenced expression of the RhDFR, RhA G, and RhNUDXin rose flowers, and affected their color, petal number, as well as fragrance, respectively. This grafting method will facilitate high-throughput functional analysis of genes in rose flowers. Importantly, it may also be applied to other woody species that are not currently amenable to VIGS by conventional leaf or plantlet/seedling infiltration methods.展开更多
Genetic control of the timing of flowering in woody plants is complex and has yet to be adequately investigated due to their long life-cycle and difficulties in genetic modification.Studies in Populus,one of the best ...Genetic control of the timing of flowering in woody plants is complex and has yet to be adequately investigated due to their long life-cycle and difficulties in genetic modification.Studies in Populus,one of the best woody plant models,have revealed a highly conserved genetic network for flowering timing in annuals.However,traits like continuous flowering cannot be addressed with Populus.Roses and strawberries have relatively small,diploid genomes and feature enormous natural variation.With the development of new genetic populations and genomic tools,roses and strawberries have become good models for studying the molecular mechanisms underpinning the regulation of flowering in woody plants.Here,we review findings on the molecular and genetic factors controlling continuous flowering in roses and woodland strawberries.Natural variation at TFL1 orthologous genes in both roses and strawberries seems be the key plausible factor that regulates continuous flowering.However,recent efforts suggest that a two-recessive-loci model may explain the controlling of continuous flowering in roses.We propose that epigenetic factors,including non-coding RNAs or chromatin-related factors,might also play a role.Insights into the genetic control of flowering time variation in roses should benefit the development of new germplasm for woody crops and shed light on the molecular genetic bases for the production and maintenance of plant biodiversity.展开更多
Seasonal variations in environmental conditions can have significant influence on flowering pattern and yield of roses. Evaluation studies were conducted to determine the flowering pattern and yield determinants of tw...Seasonal variations in environmental conditions can have significant influence on flowering pattern and yield of roses. Evaluation studies were conducted to determine the flowering pattern and yield determinants of two Hybrid Tea rose cultivars in response to seasonal variations under rain-fed conditions. The 2 × 2 × 4 factorial experiment arranged in split plot design at three replications was conducted at two seasons (dry and wet) using two cultivars (cv. “Immaculate” and “P.H. Baby”, white and red flowers respectively) supplied with poultry manure (PM) application rates at 5, 10 and 20 t/ha where the unfertilized (0 t/ha) served as control. The result showed that the year two environmental conditions of both experiment I and II significantly improved plant height, number of leaves, number of buds (CNB) and opened flowers (CNF) and flower yield (FYD). “Immaculate” was significantly (p ≤ 0.05) taller with more leaves but thinner girth. Although generally lower in NF/NB, and not different in FYD, the “P.H. Baby” had more flowers (CNB and CNF) in experiment I. Except for no significant difference in FYD and lower NF/NB recorded by all manure rates compared to control in experiment II, plants treated with 5 t/ha PM recorded more flowers (CNB and CNF). Significant first and second order interaction effects (p ≤ 0.05) showed that “Immaculate” had taller height and more leaves but “P.H. Baby” had more CNB and CNF, although both were not different in FYD. In addition, plants supplied with 5 t/ha PM rates were better in plant height, number of leaves, CNB and CNF compared to other manure rates. In conclusion, year two environmental conditions supported better crop growth and yield, “Immaculate” grew better vegetatively but both were not different in floral yield, while the 5 t/ha PM was considered the optimum manure application rate under the rain-fed condition.展开更多
OBJECTIVE To make full of resource,the bioactivity and active constituent analysis were firstly system⁃atically investigated on damask rose flower residue(DRFR).METHODS DPPH and ABTS experiments were applied to assess...OBJECTIVE To make full of resource,the bioactivity and active constituent analysis were firstly system⁃atically investigated on damask rose flower residue(DRFR).METHODS DPPH and ABTS experiments were applied to assess the antioxidant activity of DRFR.Then,column chromatography was used to purify compounds from DRFR-A,and the chemical structure was identified using nuclear magnetic resonance(NMR).The total phenolic acid content was measured by folin-ciocalteu colorimetric method and the content of gallic acid the indicator ingredient,was detected by high performance liquid chromatography(HPLC).RESULTS By DPPH and ABTS experiments,an antioxidation extract(DRFR-A)was found and displayed a high activity both on DPPH(IC50:2.760 mg·L^-1)and ABTS(IC50:2.258 mg·L^-1)compared to positive control VC.Ten compounds were isolated and identified from DRFR-A,and the most are phenolic acids.Among them,pyrogallic acid,2-phenylethyl-3,4,5-trihydroxybenzoate,p-hydroxybenzoic acid and p-hydroxy⁃phenethyl alcohol were obtained from the plant for the first time.The content of total phenolic acids and main ingredient,gallic acid,in DRFR-A was determined as 63.73%and 5.12%,respectively.CONCLUSION This study provides reliable science data and lays the foundation for the development and utilization of rose residue,and hence for the full utilization of rose resources.展开更多
On the basis of six-years’study (1986-1991) in the field of distant crossing, ten rose cultivars-elect of the to be established Rejuvenation Rose Group are recommended here for the first time. These cultivars have pe...On the basis of six-years’study (1986-1991) in the field of distant crossing, ten rose cultivars-elect of the to be established Rejuvenation Rose Group are recommended here for the first time. These cultivars have performed satisfactory ornamental characteristics and hardiness in Beijing.展开更多
文摘[Objectives] This study was conducted to develop environmentally-friendly effective vase solutions for cut rose flowers convenient for ordinary household use. [Methods] The vase life, flowering stage, flower diameter variation, petal water content, and soluble protein and malondialdehyde contents were determined on cut rose flowers regularly during the vase period, to study the fresh-keeping effects of the vase solutions prepared from such three components as sugar, aspirin and Vc according to L9(33) orthogonal table design on cut rose flowers during the vase period. [Results] 20 g/L white granulated sugar+460 mg/L aspirin+50 mg/L Vc was the best combination in this study. [Conclusions] This study provides a reference for the research on the fresh-keeping vase solutions of household cut rose flowers.
基金supported by the National Natural Science Foundation of China (31501791, 31660579, and 31360492)Leading talents in science and technology (2016HA005)funded in part by Beijing Natural Science Foundation (6162017)
文摘Rose has emerged as a model ornamental plant for studies of flower development, senescence, and morphology, as well as the metabolism of floral fragrances and colors.Virus-induced gene silencing(VIGS) has long been used in functional genomics studies of rose by vacuum infiltration of cuttings or seedlings with an Agrobacterium suspension carrying TRV-derived vectors. However, VIGS in rose flowers remains a challenge because of its low efficiency and long time to establish silencing. Here we present a novel and rapid VIGS method that can be used to analyze gene function in rose,called ‘graft-accelerated VIGS’, where axil ary sprouts are cut from the rose plant and vacuum infiltrated with Agrobacterium. The inoculated scions are then grafted back onto the plants to flower and silencing phenotypes can be observed within 5 weeks, post-infiltration. Using this new method, we successfully silenced expression of the RhDFR, RhA G, and RhNUDXin rose flowers, and affected their color, petal number, as well as fragrance, respectively. This grafting method will facilitate high-throughput functional analysis of genes in rose flowers. Importantly, it may also be applied to other woody species that are not currently amenable to VIGS by conventional leaf or plantlet/seedling infiltration methods.
基金supported by grants from the Chinese Academy of Sciences under the “Hundreds of Talents” plana grant from the “Yunnan Recruitment Program of Experts in Sciences”
文摘Genetic control of the timing of flowering in woody plants is complex and has yet to be adequately investigated due to their long life-cycle and difficulties in genetic modification.Studies in Populus,one of the best woody plant models,have revealed a highly conserved genetic network for flowering timing in annuals.However,traits like continuous flowering cannot be addressed with Populus.Roses and strawberries have relatively small,diploid genomes and feature enormous natural variation.With the development of new genetic populations and genomic tools,roses and strawberries have become good models for studying the molecular mechanisms underpinning the regulation of flowering in woody plants.Here,we review findings on the molecular and genetic factors controlling continuous flowering in roses and woodland strawberries.Natural variation at TFL1 orthologous genes in both roses and strawberries seems be the key plausible factor that regulates continuous flowering.However,recent efforts suggest that a two-recessive-loci model may explain the controlling of continuous flowering in roses.We propose that epigenetic factors,including non-coding RNAs or chromatin-related factors,might also play a role.Insights into the genetic control of flowering time variation in roses should benefit the development of new germplasm for woody crops and shed light on the molecular genetic bases for the production and maintenance of plant biodiversity.
文摘Seasonal variations in environmental conditions can have significant influence on flowering pattern and yield of roses. Evaluation studies were conducted to determine the flowering pattern and yield determinants of two Hybrid Tea rose cultivars in response to seasonal variations under rain-fed conditions. The 2 × 2 × 4 factorial experiment arranged in split plot design at three replications was conducted at two seasons (dry and wet) using two cultivars (cv. “Immaculate” and “P.H. Baby”, white and red flowers respectively) supplied with poultry manure (PM) application rates at 5, 10 and 20 t/ha where the unfertilized (0 t/ha) served as control. The result showed that the year two environmental conditions of both experiment I and II significantly improved plant height, number of leaves, number of buds (CNB) and opened flowers (CNF) and flower yield (FYD). “Immaculate” was significantly (p ≤ 0.05) taller with more leaves but thinner girth. Although generally lower in NF/NB, and not different in FYD, the “P.H. Baby” had more flowers (CNB and CNF) in experiment I. Except for no significant difference in FYD and lower NF/NB recorded by all manure rates compared to control in experiment II, plants treated with 5 t/ha PM recorded more flowers (CNB and CNF). Significant first and second order interaction effects (p ≤ 0.05) showed that “Immaculate” had taller height and more leaves but “P.H. Baby” had more CNB and CNF, although both were not different in FYD. In addition, plants supplied with 5 t/ha PM rates were better in plant height, number of leaves, CNB and CNF compared to other manure rates. In conclusion, year two environmental conditions supported better crop growth and yield, “Immaculate” grew better vegetatively but both were not different in floral yield, while the 5 t/ha PM was considered the optimum manure application rate under the rain-fed condition.
基金National Natural Science Foundation of China(331/313400531021)
文摘OBJECTIVE To make full of resource,the bioactivity and active constituent analysis were firstly system⁃atically investigated on damask rose flower residue(DRFR).METHODS DPPH and ABTS experiments were applied to assess the antioxidant activity of DRFR.Then,column chromatography was used to purify compounds from DRFR-A,and the chemical structure was identified using nuclear magnetic resonance(NMR).The total phenolic acid content was measured by folin-ciocalteu colorimetric method and the content of gallic acid the indicator ingredient,was detected by high performance liquid chromatography(HPLC).RESULTS By DPPH and ABTS experiments,an antioxidation extract(DRFR-A)was found and displayed a high activity both on DPPH(IC50:2.760 mg·L^-1)and ABTS(IC50:2.258 mg·L^-1)compared to positive control VC.Ten compounds were isolated and identified from DRFR-A,and the most are phenolic acids.Among them,pyrogallic acid,2-phenylethyl-3,4,5-trihydroxybenzoate,p-hydroxybenzoic acid and p-hydroxy⁃phenethyl alcohol were obtained from the plant for the first time.The content of total phenolic acids and main ingredient,gallic acid,in DRFR-A was determined as 63.73%and 5.12%,respectively.CONCLUSION This study provides reliable science data and lays the foundation for the development and utilization of rose residue,and hence for the full utilization of rose resources.
文摘On the basis of six-years’study (1986-1991) in the field of distant crossing, ten rose cultivars-elect of the to be established Rejuvenation Rose Group are recommended here for the first time. These cultivars have performed satisfactory ornamental characteristics and hardiness in Beijing.
文摘为了揭示野蔷薇(Rose multiflora Thunb.)花瓣开放过程中花瓣扩展相关基因的表达情况,试验以野蔷薇为材料,通过RNA-seq技术分析不同开放阶段的花瓣中差异基因的表达,为进一步深入研究野蔷薇花瓣转录水平上的发育调控提供依据。结果表明:转录组分析组装得到52865个单基因(Unigenes),通过对NR数据库的比对,功能注释匹配物种中,与野蔷薇匹配的物种前3位为野草莓(Fragaria vesca subsp.vesca)(36.17%)、粳稻(Oryza sativa Japonica)(13.65%)、棉花(Gossypium hirsutum)(4.04%)。对花瓣开放的3个比较组(FB_CP vs FB_GP,FB_YP vs FB_CP,OF_WP vs FB_YP)的差异基因比较,各阶段的差异基因分别为642、902、1899个。通过对不同开花阶段花瓣扩展相关基因表达分析,可能受EXP,XTH基因家族及细胞壁合成、修饰和水解相关基因XYL,CES的作用。