Effect of Rosehip (<i>Rosa Canina</i>) Extracts on Human Brain Tumor Cell Proliferation and Apoptosis

Rosehips are blossoms from the wild rose (Rosa canina) and are commonly used as an herbal remedy. Previous reports have shown that extracts made from rosehip plants are able to reduce cell proliferation of cancer cells. In this study, we investigated the efficacy of rosehip extracts in preventing cell proliferation of three human glioblastoma cell lines A-172, U-251 MG and U-1242 MG cell lines. Each of the glioblastoma cell lines treated with rosehip extracts (1 mg/mL-25 ng/mL) demonstrated a significant decrease in cell proliferation. The rosehip extract-mediated decrease in cell proliferation was equal to or better than the decrease of cell proliferation observed when inhibitors of the MAPK (U0126, 10 μM) or AKT (LY294002, 20 μM) signaling pathways were utilized. Additionally, pretreatment of the these cell lines with Rosehip extracts (1 mg/mL-25 ng/mL) selectively decreased AKT, MAPK, and p70S6K phosphorylation suggesting these extracts prevent glioblastoma multiforme cell proliferation by blocking both the MAPK and AKT signaling mechanisms. Results from colorimetric cell death assays, cell cycle analysis by flow cytometry, as well as western blot studies demonstrate that rosehip extracts inhibit cell proliferation but do not promote apoptosis. Moreover, rosehip extracts were able to increase the efficacy of Temozolomide, a chemotherapeutic agent used to treat patients with glioblastomas. Surprisingly, rosehip extracts demonstrated a greater inhibition of cell proliferation than in combination with Temozolomide (100 μM) or Temozolomide as a single agent. Taken together these data suggest that rosehip extracts are capable of decreasing glioblastoma cell proliferation without promoting apoptosis and demonstrate a greater cell proliferation inhibitory effect than Temozolomide. More importantly, rosehip extracts may serve as an alternative or compliment to current chemotherapeutic regimens for glioblastomas.

Evolution of Post-Surgical Scars Treated with Pure Rosehip Seed Oil

The rosehip seed oil (RHO), obtained from different plant species of the genus Rosa, is one of the compounds used empirically for cosmetic improvement of skin scarring. Despite its widespread use in clinical practice, there are few studies evaluating the activity of this compound on the clinical course of cutaneous scars. The aim of this study was to determine the effect of Repavar&reg?rosehip oil on improvement of post-surgical skin scars. One comparative, single-center, prospective clinical trial was carried out in 108 patients undergoing cutaneous surgery procedures in the Dermatology Service of University Hospital of Gran Canaria Dr. Negrín (Spain). Subjective parameters (erythema, discoloration, atrophy and hypertrophy) were evaluated at 6 and 12 weeks on 76 adults who treated scars with pure RHO twice a day (test group), 32 patients with not treatment (control group), and completed the study. Lesser degree of erythema was observed at 6 and 12 weeks in treated-patients compared with the control group and decreased discoloration and atrophy at 12 weeks, with statistically significant differences in all cases (p < 0.05). This study demonstrates that the RHO Repavar&reg?is useful for cosmetic improvement on erythema, discoloration and atrophyof post-surgical skin scars, getting a better overall evolution and appearance thereof.

基于总糖、可溶性蛋白和酚酸含量的新疆蔷薇果实营养品质评价

【目的】深入挖掘新疆地区的蔷薇果实种质资源,了解其主要营养成分差异,为蔷薇果品质改良及开发利用提供参考依据。【方法】以单叶蔷薇、密刺蔷薇、宽刺蔷薇和弯刺蔷薇等10种新疆蔷薇果为试验材料,测定果实中总糖、可溶性蛋白和酚酸的含量并进行方差分析、相关性分析及聚类分析,运用隶属函数法对10种新疆蔷薇果实进行差异分析和营养品质综合评价。【结果】单叶蔷薇果实的总糖和酚酸含量最高,分别为125.54、9.09 mg·g^(-1),现代月季‘天山祥云’果实的总糖和酚酸含量最低,分别为102.90、4.78 mg·g^(-1);可溶性蛋白含量最高的是‘天山祥云’,为46.68 mg·g^(-1),最低的是宽刺蔷薇,为28.51 mg·g^(-1)。相关性分析表明,蔷薇果的酚酸与总糖含量呈极显著正相关,可溶性蛋白与酚酸和总糖均呈现极显著负相关。聚类分析结果显示,10种新疆蔷薇果可分为3个类群,第1类是弯刺蔷薇、疏花蔷薇1、宽刺蔷薇、托木尔蔷薇和尖刺蔷薇;第2类是单叶蔷薇;第3类包括疏花蔷薇2、‘天山祥云’、密刺蔷薇和伊犁蔷薇,可根据不同需求和目标选择相应的果实类群。平均隶属函数值可排序为:单叶蔷薇>密刺蔷薇>弯刺蔷薇>伊犁蔷薇>托木尔蔷薇>尖刺蔷薇>疏花蔷薇1>宽刺蔷薇>疏花蔷薇2>‘天山祥云’。【结论】蔷薇属果实具有较高的食用价值和药用价值,但不同蔷薇果中总糖、可溶性蛋白和酚酸含量差异较大,该研究结果可为蔷薇属果实的开发利用提供一定的参考依据和理论支撑。

玫瑰果油神经酰胺(NP)的合成方法及其用途研究

以天然来源的玫瑰果油作为原料,合成复合的神经酰胺。通过HaCat细胞MMP-1表达量实验、B16细胞黑色素含量实验和DPPH自由基清除实验对玫瑰果油神经酰胺(NP)进行功效评价。实验结果表明,玫瑰果油神经酰胺(NP)具备抗衰老、抗氧化、美白的功效,未来在化妆品领域将有更广泛的应用前景。

大孔树脂分离纯化玫瑰果多酚及其抗氧化性

为了研究大孔树脂对玫瑰果多酚的纯化效果,该试验以玫瑰果多酚粗提液为原料,利用大孔树脂吸附法对其进行纯化,并对其体外抗氧化活性进行了研究。结果表明,大孔吸附树脂纯化较佳工艺为:在20℃条件下,用质量浓度为0.80 mg/m L的玫瑰果多酚粗提液(p H值5.8)以1 m L/min的流速上样200 m L;吸附平衡后用少量蒸馏水洗至洗脱液无色,后用70 m L体积分数为70%的乙醇溶液,以1.5 m L/min的流速进行动态洗脱,洗脱峰相对集中、对称,无拖尾。纯化后,总酚质量分数由纯化前的122.90 mg/g提高到399.42 mg/g。体外抗氧化活性的研究表明:清除1,1-二苯基-2-苦基肼自由基的作用效果顺序为维生素C>纯化多酚>2,6-二叔丁基-4-甲基苯酚(Butylated hydroxytoluene,BHT)>粗多酚;清除超氧阴离子自由基的作用效果顺序为维生素C>BHT>纯化多酚>粗多酚;清除羟基自由基的作用效果顺序为维生素C>纯化多酚>BHT>粗多酚。研究结果为玫瑰果多酚的进一步利用提供依据。

玫瑰果格瓦斯发酵工艺优化

为提高玫瑰果资源的利用率,以玫瑰果和两种麦芽为原料制作一款新型玫瑰果格瓦斯。引入库德里阿兹威毕赤酵母(Pichia kudriavzevii)协助酿酒酵母(Saccharomyces cerevisiae)和植物乳杆菌(Lactobacillus plantarum)进行发酵以改进传统发酵工艺。采用单因素试验和响应面试验设计对玫瑰果格瓦斯的发酵工艺进行优化。结果表明,最优发酵工艺为初始可溶性固形物含量8°Bx,发酵温度26℃,发酵时间20 h,三种菌株总接种量2%,接种比为1∶1∶1。此优化条件下,玫瑰果格瓦斯酒精度为1.09%vol,总酸含量为4.02 g/L。