Objective:To find out the extent of duffy-binding-like(DBL) a gene diversity and the resetting potential of the parasite population in association with severe malaria.Methods:Genotyping of DBLαdomain was done by PCR ...Objective:To find out the extent of duffy-binding-like(DBL) a gene diversity and the resetting potential of the parasite population in association with severe malaria.Methods:Genotyping of DBLαdomain was done by PCR using three sets of primers(FR,F1R2 and F2R2) and the rosetting frequency was assessed by parasite culture followed by ethidium bromide staining and visualization under a fluorescent microscope.Results:The significant association of high parasite density with severe malaria and the positive correlation between rosetting frequency and parasite density in vivo(P = 0.613,P【0.0001) were observed.Moreover,the parasite strains having multiple fragments of F2R2 region and’b’variant of FR region of DBL 1-αshowed increased rosetting frequency and supported the strain specific association of disease severity. Conclusions:The findings suggest that rosetting mediated higher parasitemia might have contributed to the development of severe disease.As the rosetting domain of Plasmodium falciparum erythrocyte membrane protein 1(HEMP1),the DBL a binds to multiple host receptors; the significant association of multiple fragments of F2R2 region with severe malaria suggests several receptor-ligand interactions as the possible mechanisms of pathogenesis.Alternatively, the high percentage distribution of smaller fragments with mild malaria suggests the lack of adequate rosetting epitopes that might have contributed to low rosetting frequency in mild malaria.展开更多
＂未来实质上扎根于每个人对于生活的期望＂,Linge Roset的总监Pierre and Michel Roset以寄于回忆、趣事的写实手法架起了Linge Roset过去与未来的桥梁。Linge Roset的设计思维是：新鲜事物会培育设计文化。比如,菲利普·尼格罗的C...＂未来实质上扎根于每个人对于生活的期望＂,Linge Roset的总监Pierre and Michel Roset以寄于回忆、趣事的写实手法架起了Linge Roset过去与未来的桥梁。Linge Roset的设计思维是：新鲜事物会培育设计文化。比如,菲利普·尼格罗的Confluences座椅系列和弗朗索瓦·巴其特的罗马人储藏类家具系列项目都体现了Linge Roset的情感概念。展开更多
文摘Objective:To find out the extent of duffy-binding-like(DBL) a gene diversity and the resetting potential of the parasite population in association with severe malaria.Methods:Genotyping of DBLαdomain was done by PCR using three sets of primers(FR,F1R2 and F2R2) and the rosetting frequency was assessed by parasite culture followed by ethidium bromide staining and visualization under a fluorescent microscope.Results:The significant association of high parasite density with severe malaria and the positive correlation between rosetting frequency and parasite density in vivo(P = 0.613,P【0.0001) were observed.Moreover,the parasite strains having multiple fragments of F2R2 region and’b’variant of FR region of DBL 1-αshowed increased rosetting frequency and supported the strain specific association of disease severity. Conclusions:The findings suggest that rosetting mediated higher parasitemia might have contributed to the development of severe disease.As the rosetting domain of Plasmodium falciparum erythrocyte membrane protein 1(HEMP1),the DBL a binds to multiple host receptors; the significant association of multiple fragments of F2R2 region with severe malaria suggests several receptor-ligand interactions as the possible mechanisms of pathogenesis.Alternatively, the high percentage distribution of smaller fragments with mild malaria suggests the lack of adequate rosetting epitopes that might have contributed to low rosetting frequency in mild malaria.
文摘＂未来实质上扎根于每个人对于生活的期望＂,Linge Roset的总监Pierre and Michel Roset以寄于回忆、趣事的写实手法架起了Linge Roset过去与未来的桥梁。Linge Roset的设计思维是：新鲜事物会培育设计文化。比如,菲利普·尼格罗的Confluences座椅系列和弗朗索瓦·巴其特的罗马人储藏类家具系列项目都体现了Linge Roset的情感概念。
