Determination of Gallic Acid in Rotala rotundifolia and Shuijia Zhixue Capsule by HPLC

[Objectives] To establish a method for the determination of gallic acid in Shuijia Zhixue capsule and Rotala rotundifolia .[Methods] High performance liquid chromatography (HPLC) and Phenomenex C 18 column were used, the mobile phase was 0.1% phosphoric acid∶ methanol=85∶ 15, the flow rate was 0.5 mL/min, and the detection wavelength was 270 nm.[Results] In the range of (2-25)μg/mL , the concentration of gallic acid had a good linear relationship with the peak area, r =0.990 8;the average recovery was 96.88%.[Conclusions] The method was simple, sensitive and reproducible. It provided a basis for the analysis of gallic acid in Rotala rotundifolia and Shuijia Zhixue capsule and the establishment of quality detection methods in the future.

Hemostatic and Anti-inflammatory Activities and High Performance Liquid Chromatography Analysis of Rotala rotundifolia and Its Preparations

[Objectives] To evaluate the hemostatic and anti-inflammatory activities of water extract and ethanol extract of Rotala rotundifolia and Shuijia Zhixue capsule. [Methods]The hemostatic activity of the extracts was evaluated by the changes of the bleeding time( BT),coagulation time( CT) and coagulation parameters including prothrombin time( PT),activated partial thromboplastin time( APTT) and thrombin time( TT) in mice or rats. The anti-inflammatory activity of the extracts was evaluated by carrageenan-induced foot edema,arachidonic acidinduced ear edema and xylene-induced ear edema models in rats. The content of gallic acid in ethanol extracts of R. rotundifolia and Shuijia Zhixue capsule was studied by HPLC to explore the correlation between gallic acid and anti-inflammatory activity. [Results]In hemostasis experiment,the ethanol extracts of R. rotundifolia and Shuijia Zhixue capsules showed significant shortening effects on BT,CT,PT and TT( P <0. 01-0. 05). The water extracts of R. rotundifolia and Shuijia Zhixue capsules had significant shortening effects on CT,PT and TT( P <0. 05),while the water extracts of R. rotundifolia had no hemostasis effect. In anti-inflammatory experiment in vivo,the two ethanol extracts showed significant shortening effects on CT,PT and TT and in the induction test of carrageenan,arachidonic acid and xylene,the inhibitory effects were significant( P < 0. 01-0. 05). The water extract from Shuijia Zhixue capsules had significant inhibitory effects on carrageenan-induced foot edema in rats( P < 0. 05),but the anti-inflammatory effects of water extract from R. rotundifolia were not significant. The content of gallic acid in R. rotundifolia and Shuijia Zhixue capsules were( 0. 318 62 ± 0. 009 8) and( 6. 295 2 ± 0. 162 6) mg/g,respectively.Among them,the content of gallic acid in Shuijia Zhixue capsule was significantly correlated with the anti-inflammatory activity of carrageenaninduced foot edema model. [Conclusions] Ethanol extract of R. rotundifolia and Shuijia Zhixue capsules has strong hemostatic and anti-inflammatory activities,and can be used in the treatment of traditional diseases such as bleeding and inflammation.

沉水态圆叶节节菜的化感抑藻作用

【目的】研究沉水态圆叶节节菜Rotala rotundifolia的化感抑藻效果及潜在抑藻机制,可为圆叶节节菜在藻类水华治理工程应用中提供理论基础。【方法】以铜绿微囊藻Microcystis aeruginosa为代表性藻种,采用超声辅助溶剂浸提方式,研究水、乙醇和石油醚3种溶剂提取物的抑藻性能,并从叶绿素a(Chl a)质量浓度、最大光化学效率(F_(v)/F_(m))及超氧化物歧化酶(SOD)活性等方面比较了圆叶节节菜与6种常见沉水植物的抑藻作用。【结果】圆叶节节菜3种溶剂提取物的抑藻能力从大到小依次为石油醚、乙醇、水,石油醚提取物对铜绿微囊藻有显著的抑制作用(P<0.05),抑制率最高可达99.9%;量效分析表明:圆叶节节菜石油醚提取物有低促高抑现象,半最大效应质量浓度(EC_(50))为11.56 g·L^(-1)(第5天);与常见的沉水植物抑藻效果相比,圆叶节节菜和穗花狐尾藻Myriophyllum spicatum处理的Chl a质量浓度显著低于其他处理(P<0.05,第1天);所有植物处理的F_(v)/F_(m)均显著低于对照(P<0.05);圆叶节节菜和水盾草Cabomba caroliniana的SOD活性分别是对照的1.48和1.53倍,穗花狐尾藻的SOD活性显著下降(P<0.05),而苦草Vallisneria natans、金鱼藻Ceratophyllum demersum、伊乐藻Elodea nuttallii和轮叶黑藻Hydrilla verticillata处理与对照无显著差异。【结论】沉水态圆叶节节菜石油醚提取物具有较强的抑藻效应,表现为减少铜绿微囊藻细胞Chl a质量浓度、降低光化学效率及加剧氧化损伤等,在微囊藻水华防控方面具有较强的应用潜力。

圆叶节节菜的化学成分研究

从圆叶节节菜地上部分乙酸乙酯层中分离得到8个化合物,经波谱学方法分别鉴定为pomolic acid3β-acetate(1),7-oxo-β-sitosterol(2),6β-hydroxystigmast-4-en-3-one(3),esculetin(4),phyllemblin(5),1,2-O-digalloylglycerol(6),apigenin(7),quercetin(8),以上化合物均为首次从该植物中分离得到。化合物(6)在GK,SIRT1激动活性及DPPIV,11β-HSD抑制活性等抗糖尿病体外活性测试中均无明显的活性。

圆叶节节菜不同体积分数乙醇提取物的止血和肝保护作用研究

目的研究提取溶剂中不同体积分数乙醇对圆叶节节菜止血作用的影响。方法采用毛细管法、半自动凝血分析仪和全自动三分群血液分析仪测定凝血时间(CT)、凝血酶原时间(PT)、活化部分凝血活酶时间(APTT)、血浆凝血酶时间(TT)、血小板数(PLT)和血小板分布宽度(PDW),评价圆叶节节菜不同体积分数(5%,10%,20%,50%,80%和100%)乙醇提取物体内外止血作用;采用四氯化碳(CCl 4)诱导小鼠急性肝损伤模型,测定小鼠血清谷丙转氨酶(ALT)、总蛋白(TP)和总胆红素(TB)的水平;以具有最佳止血效果的圆叶节节菜提取物作为研究对象,采用PT、APTT和TT测定法研究量效关系。结果随着乙醇体积分数的提高,圆叶节节菜乙醇提取物产率下降;乙醇体积分数为50%及以上的圆叶节节菜提取物中CT、PT、APTT和TT显著缩短;与对照组比较,乙醇体积分数为50%,80%和100%的提取物中PLT显著增加,但各治疗组的PDW差别不显著;各提取物组的TP水平变化不大,与对照组比较,乙醇体积分数为5%,10%和100%提取物组的TB水平显著下降,乙醇体积分数高于20%的各提取物组ALT水平显著降低;乙醇体积分数为100%提取物组止血作用最佳,且具有剂量依赖性。结论提取溶剂中乙醇的体积分数对圆叶节节菜止血作用有重要影响,高体积分数乙醇提取物具有显著的止血作用。

彝药水指甲药材及其制剂β-甾醇的含量测定

建立一种用紫外分光光度法以测定彝药水指甲药材及其制剂β-谷甾醇含量。以浓硫酸为显色剂,采用紫外分光光度法在314 nm下测定β-谷甾醇含量。在630μg/mL浓度范围内,β-谷甾醇溶液的吸光度线性关系良好,相关系数为0.9991;水指甲药材β-谷甾醇的平均含量为(1.782±0.004)g·kg^(-1),水甲痔血胶囊β-谷甾醇的含量为(2.373±0.008)g·kg^(-1);水指甲药材平均回收率为99.64%(RSD=1.40%);水甲痔血胶囊的平均回收率为99.76%(RSD=1.34%)。该方法简便快捷、数据准确可靠,可用于测定水指甲及其制剂中β-谷甾醇含量。

我国千屈菜科植物化学成分研究进展

水指甲(圆叶节节菜)作为彝族民间习用药材,几年来逐渐受到人们的关注,为了了解其中的化学成分,结合相关文献,对水指甲所属的千屈菜科化学成分作了全面介绍,重点对生物碱类、黄酮类、三萜类、鞣质类、香豆素类和萘衍生物类进行了详细的介绍,为千屈菜科植物的开发利用提供参考。

圆叶节节菜提取物的抗菌活性及机理研究

目的 研究圆叶节节菜提取物的抑菌活性及作用机理。方法 采用纸片扩散法和二倍稀释2,3,5-氯化三苯基四氮唑染色法测定金黄色葡萄球菌和铜绿假单胞菌的抑菌圈直径、最小抑菌浓度(MIC)和最小杀菌浓度(MBC);并通过测定电导率、核酸、碱性磷酸酶活性和蛋白质的含量,初步阐明其抑菌机理。结果 圆叶节节菜提取物对金黄色葡萄球菌和铜绿假单胞菌的抑菌圈直径为9.02~11.46 mm,其MIC和MBC分别为0.625、1.25 g·mL-1;用提取物处理后,菌胞外电导率、核酸、碱性磷酸酶和蛋白质的浓度明显增加,表明提取物增加了菌细胞的通透性,导致菌胞内物质泄露,最终导致菌生长受到抑制。结论 圆叶节节菜提取物具有抗菌活性,其抗菌作用可能与膜壁屏障受损有关。

圆叶节节菜中Megastigmane类型的化合物(英文)

目的:研究圆叶节节菜的化学成分。方法:运用硅胶柱色谱技术分离、光谱技术鉴定结构。结果:从其地上部分分离得到两个Megastigmane类型化合物。结论:化合物1(Rotundifoline A)是一个新化合物。