AIM: To clarify the mechanism underlying the anti-diabetic activities of cortex cinnamomi extract (CCE). METHODS: To induce in vivo diabetes, mice were injected with streptozotocin (STZ) via a tail vein (100 mg STZ/kg...AIM: To clarify the mechanism underlying the anti-diabetic activities of cortex cinnamomi extract (CCE). METHODS: To induce in vivo diabetes, mice were injected with streptozotocin (STZ) via a tail vein (100 mg STZ/kg body weight). To determine the effects of CCE, mice were administered CCE twice daily for 7 d by oral gavage starting 1 wk before the STZ injection. Blood glucose and plasma insulin concentration were measured as an index of diabetes. Also, to induce cytotoxicity of RINm5F cells, we treated with cytokines (IL-1β(2.0 ng/mL) and IFN-γ(100 U/mL)). Cell viability and nitric oxide production were measured colorimetrically. Inducible nitric oxide synthase (iNOS) mRIMA and protein expression were determined by RT-PCR and Western blotting, respectively. The activation of NF-κB was assayed by using gel mobility shift assays of nuclear extracts. RESULTS: Treatment of mice with STZ resulted in hyperglycemia and hypoinsulinemia, which was further evidenced by immunohistochemical staining of islets. However, the diabetogenic effects of STZ were completely prevented when mice were pretreated with CCE. The inhibitory effect of CCE on STZ-induced hyperglycemia was mediated through the suppression of iNOS expression. In rat insulinoma RINm5F cells, CCE completely protected against interleukin-1βand interferon-γ-mediated cytotoxicity. Moreover, RINm5F cells incubated with CCE showed significant reductions in interleukin-lp and interferon-γ-induced nitric oxide production and in iNOS mRNA and protein expression, and these findings correlated well with in vivo observations. CONCLUSION: The molecular mechanism by which CCE inhibits iNOS gene expression appears to involve the inhibition of NF-κB activation. These results reveal the possible therapeutic value of CCE for the prevention of diabetes mellitus progression.展开更多
Cinnamomi Cortex(Rougui)has a lot of functions,including dissipating cold,relieving pain,benefiting joints,tonifying fire and assisting yang,warming blood vessels,dispersing abscesses and nodules and vaporizing fluid ...Cinnamomi Cortex(Rougui)has a lot of functions,including dissipating cold,relieving pain,benefiting joints,tonifying fire and assisting yang,warming blood vessels,dispersing abscesses and nodules and vaporizing fluid and humor.Among all functions,the wonderful use of Cinnamomi Cortex is in conducting the fire back to the origin.It can conduct the fire of triple energizers,which is not possessed by other drugs and is unique to Cinnamomi Cortex.Through observations and practices of some special medical records,it is found that the fire conducting of Cinnamomi Cortex can play a key and decisive role.展开更多
Cinnamomi Cortex was a material as medicinal and edible resources and it has been demonstrated that Cinnamomi Cortex aqueous extract(CCAE)possess hypoglycemic effect,but there were no active compounds identified as qu...Cinnamomi Cortex was a material as medicinal and edible resources and it has been demonstrated that Cinnamomi Cortex aqueous extract(CCAE)possess hypoglycemic effect,but there were no active compounds identified as quality standard of CCAE to be used in the investigate of hypoglycemic activity.Hence,in this study,the quality standard and the hypoglycemic effect of CCAE were investigated.Cinnamaldehyde and coumarin as the quality control in CCAE were analyzed by high performance liquid chromatography(HPLC)method.The results showed that the average yield of extraction was 10.06%.The concentration of coumarin was linear with peak area ranging from 0.02–1.00μg/mL(r=0.9996,n=6)and that of cinnamaldehyde also demonstrated a good linear relationship with peak area in the range of 1.05–52.50μg/mL(r=0.9997,n=6).The average recovery rate of coumarin and cinnamaldehyde was 99.42%and 100.11%,respectively.The investigation of hypoglycemic effect revealed that CCAE could improve weight loss,lower liver and pancreas indices,improve organ damage caused by diabetes,and lower fasting blood glucose,glycosylated serum protein(GSP)and total cholesterol(TC)levels.The results showed that CCAE can reduce fasting blood glucose and blood lipid levels in diabetic mice.展开更多
基金Supported by the Regional Research Center Program of the Korean Ministry of Education & Human Resources Development through the Center for Healthcare Technology Development
文摘AIM: To clarify the mechanism underlying the anti-diabetic activities of cortex cinnamomi extract (CCE). METHODS: To induce in vivo diabetes, mice were injected with streptozotocin (STZ) via a tail vein (100 mg STZ/kg body weight). To determine the effects of CCE, mice were administered CCE twice daily for 7 d by oral gavage starting 1 wk before the STZ injection. Blood glucose and plasma insulin concentration were measured as an index of diabetes. Also, to induce cytotoxicity of RINm5F cells, we treated with cytokines (IL-1β(2.0 ng/mL) and IFN-γ(100 U/mL)). Cell viability and nitric oxide production were measured colorimetrically. Inducible nitric oxide synthase (iNOS) mRIMA and protein expression were determined by RT-PCR and Western blotting, respectively. The activation of NF-κB was assayed by using gel mobility shift assays of nuclear extracts. RESULTS: Treatment of mice with STZ resulted in hyperglycemia and hypoinsulinemia, which was further evidenced by immunohistochemical staining of islets. However, the diabetogenic effects of STZ were completely prevented when mice were pretreated with CCE. The inhibitory effect of CCE on STZ-induced hyperglycemia was mediated through the suppression of iNOS expression. In rat insulinoma RINm5F cells, CCE completely protected against interleukin-1βand interferon-γ-mediated cytotoxicity. Moreover, RINm5F cells incubated with CCE showed significant reductions in interleukin-lp and interferon-γ-induced nitric oxide production and in iNOS mRNA and protein expression, and these findings correlated well with in vivo observations. CONCLUSION: The molecular mechanism by which CCE inhibits iNOS gene expression appears to involve the inhibition of NF-κB activation. These results reveal the possible therapeutic value of CCE for the prevention of diabetes mellitus progression.
基金Project of Inheritance and Instruction Studio for Zhou Junhuai,an Old Chinese Medicine Expert at the Grassroots Level in Hainan Province。
文摘Cinnamomi Cortex(Rougui)has a lot of functions,including dissipating cold,relieving pain,benefiting joints,tonifying fire and assisting yang,warming blood vessels,dispersing abscesses and nodules and vaporizing fluid and humor.Among all functions,the wonderful use of Cinnamomi Cortex is in conducting the fire back to the origin.It can conduct the fire of triple energizers,which is not possessed by other drugs and is unique to Cinnamomi Cortex.Through observations and practices of some special medical records,it is found that the fire conducting of Cinnamomi Cortex can play a key and decisive role.
基金Research on Precision Nutrition and Health FoodDepartment of Science and Technology of Henan Province(CXJD2021006)+2 种基金Science and Technology Development Program of Henan Province(222102310481202102110136)Key University Science Research Project of Henan Province(20A360001).
文摘Cinnamomi Cortex was a material as medicinal and edible resources and it has been demonstrated that Cinnamomi Cortex aqueous extract(CCAE)possess hypoglycemic effect,but there were no active compounds identified as quality standard of CCAE to be used in the investigate of hypoglycemic activity.Hence,in this study,the quality standard and the hypoglycemic effect of CCAE were investigated.Cinnamaldehyde and coumarin as the quality control in CCAE were analyzed by high performance liquid chromatography(HPLC)method.The results showed that the average yield of extraction was 10.06%.The concentration of coumarin was linear with peak area ranging from 0.02–1.00μg/mL(r=0.9996,n=6)and that of cinnamaldehyde also demonstrated a good linear relationship with peak area in the range of 1.05–52.50μg/mL(r=0.9997,n=6).The average recovery rate of coumarin and cinnamaldehyde was 99.42%and 100.11%,respectively.The investigation of hypoglycemic effect revealed that CCAE could improve weight loss,lower liver and pancreas indices,improve organ damage caused by diabetes,and lower fasting blood glucose,glycosylated serum protein(GSP)and total cholesterol(TC)levels.The results showed that CCAE can reduce fasting blood glucose and blood lipid levels in diabetic mice.
