Phytolacca acinosa Roxb. （P acinosa） is a recently discovered manganese hyperaccumulator plant from southern China. It is a good candidate for phytoremediation of manganese（Mn） polluted soil for its high biomass a...Phytolacca acinosa Roxb. （P acinosa） is a recently discovered manganese hyperaccumulator plant from southern China. It is a good candidate for phytoremediation of manganese（Mn） polluted soil for its high biomass and fast growth. Knowledge of the tissue localization and identification of heavy metals can provide essential information on metal toxicity and bioaccumulation mechanisms. Synchrotron radiation X-ray fluorescence spectroscopy （SRXRF） microprobe was used in this study to investigate the cellular distributions of Mn and other elements in root, stem, leaf, petiole and midrib of P. acinosa. The highest Mn content was found in the vascular tissues of root, stem, petiole and midrib. Cortex in root played a key role in Mn absorption and Mn was limited in the vascular bundle during the process of transportation in stem. Moreover, Mn content in leaf epidermis was higher than that in mesophyll, which suggested that the sequestration of Mn in leaf epidermis might be one of the detoxification mechanisms of P. acinosa. The significance of other elemental （such as P, S, K, Ca, Fe, Zn and Cu） distribution patterns and the correlation with Mn were also discussed.展开更多
Objective:To investigate the cytotoxic effect of ethanol extract of the stem bark of asam kandis[Garcinia cowa Roxb.(G.cowa)]on T47 D breast cancer cell line.Methods:The cytotoxicity of ethanol extract was carried out...Objective:To investigate the cytotoxic effect of ethanol extract of the stem bark of asam kandis[Garcinia cowa Roxb.(G.cowa)]on T47 D breast cancer cell line.Methods:The cytotoxicity of ethanol extract was carried out against human breast cancer cell line(T47D) by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide colorimetric assay.The extract was added at various concentrations(0.1.1,10 and 100 μg/mL).The level of cytotoxicity was determined by calculating the level of IC_(50),that was based on the percentage of the cell death after 24 h treatment with the extract.Cell morphological changes were observed by using inverted microscope.Results:The 3-(4.5-dimelhylthiazol-2-yl)-2.5-diphenyltelrazolium bromide assay showed that ethanol extract of G.cowa exhibited significant cytotoxic effect on T47 D with IC_(50) value of(5.10+1.68) μg/mL.Morphological alteration of the cell lines after exposure to ethanol extract of G.cowa was observed under phase contrast microscope in a dosc-dependent manner.ConclusionsThe results suggest the possible use of ethanol extract of asam kandis for preparing herbal medicine for cancer-related ailments.展开更多
The development prospect of Pennisetum Sinese Roxb. is receiving much concern of domestic researchers. In this paper,literature of Pennisetum Sinese Roxb. was taken as basis,and bibliometric analysis method was used t...The development prospect of Pennisetum Sinese Roxb. is receiving much concern of domestic researchers. In this paper,literature of Pennisetum Sinese Roxb. was taken as basis,and bibliometric analysis method was used to make statistics of distribution of years,research institutions,authors,and research fields. The results showed that research fields of Pennisetum Sinese Roxb. in China were mainly ecological restoration,accounting for 13. 27% of the total literature; the research took on dynamic and diversified cooperation trend. In recent 5 years,the number of papers significantly increased,accounting for 93. 04% of the total literature. The distribution of authors and regions were relatively concentrated,but the overall level of research is low.展开更多
文摘采用可视化分析的方式对广西特色药材两面针[Zanthoxylum nitidum(Roxb.)DC.]的研究进展和主要研究方向进行分析,预测该领域未来发展走向,以期为后续深入研究两面针提供理论参考。以“两面针”为检索词,从中国知网(CNKI)中整合1971—2022年的相关中文文献,运用CiteSpace V 5.7 R5软件从作者、机构、关键词等主题进行可视化分析。结果表明,筛选、排除与主题无关的文献后共纳入788篇文献,其中,发文量最多的作者是刘华钢,共发文65篇;发文较多的机构集中于广西,广西医科大学为研究两面针最多的机构,且有明显的地域性;高校与企业间合作较多,如广州中医药大学与华润三九医药股份有限公司等;关键词分析显示对两面针的研究主要集中在化学成分、药理作用及临床应用。结合高效液相色谱法对两面针及其相关中药复方以及产品的质量控制是研究热点。氯化两面针碱的抗肿瘤活性以及结合高效液相色谱法进行质量控制研究将是该领域的热门方向。
文摘Phytolacca acinosa Roxb. （P acinosa） is a recently discovered manganese hyperaccumulator plant from southern China. It is a good candidate for phytoremediation of manganese（Mn） polluted soil for its high biomass and fast growth. Knowledge of the tissue localization and identification of heavy metals can provide essential information on metal toxicity and bioaccumulation mechanisms. Synchrotron radiation X-ray fluorescence spectroscopy （SRXRF） microprobe was used in this study to investigate the cellular distributions of Mn and other elements in root, stem, leaf, petiole and midrib of P. acinosa. The highest Mn content was found in the vascular tissues of root, stem, petiole and midrib. Cortex in root played a key role in Mn absorption and Mn was limited in the vascular bundle during the process of transportation in stem. Moreover, Mn content in leaf epidermis was higher than that in mesophyll, which suggested that the sequestration of Mn in leaf epidermis might be one of the detoxification mechanisms of P. acinosa. The significance of other elemental （such as P, S, K, Ca, Fe, Zn and Cu） distribution patterns and the correlation with Mn were also discussed.
基金Supported by Hibah Doktor scheme of Directorate General of Higher Education,Ministry of Education and Culture,Republic of Indonesia(Grant No.DIPA 09/UN.16/D-DD/2014)
文摘Objective:To investigate the cytotoxic effect of ethanol extract of the stem bark of asam kandis[Garcinia cowa Roxb.(G.cowa)]on T47 D breast cancer cell line.Methods:The cytotoxicity of ethanol extract was carried out against human breast cancer cell line(T47D) by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide colorimetric assay.The extract was added at various concentrations(0.1.1,10 and 100 μg/mL).The level of cytotoxicity was determined by calculating the level of IC_(50),that was based on the percentage of the cell death after 24 h treatment with the extract.Cell morphological changes were observed by using inverted microscope.Results:The 3-(4.5-dimelhylthiazol-2-yl)-2.5-diphenyltelrazolium bromide assay showed that ethanol extract of G.cowa exhibited significant cytotoxic effect on T47 D with IC_(50) value of(5.10+1.68) μg/mL.Morphological alteration of the cell lines after exposure to ethanol extract of G.cowa was observed under phase contrast microscope in a dosc-dependent manner.ConclusionsThe results suggest the possible use of ethanol extract of asam kandis for preparing herbal medicine for cancer-related ailments.
基金Supported by Emergency Management Project of National Natural Science Foundation(31160292)
文摘The development prospect of Pennisetum Sinese Roxb. is receiving much concern of domestic researchers. In this paper,literature of Pennisetum Sinese Roxb. was taken as basis,and bibliometric analysis method was used to make statistics of distribution of years,research institutions,authors,and research fields. The results showed that research fields of Pennisetum Sinese Roxb. in China were mainly ecological restoration,accounting for 13. 27% of the total literature; the research took on dynamic and diversified cooperation trend. In recent 5 years,the number of papers significantly increased,accounting for 93. 04% of the total literature. The distribution of authors and regions were relatively concentrated,but the overall level of research is low.
