Rubratoxin B诱导人绒癌细胞株凋亡及Bcl-2 mRNA表达的研究

目的:定量分析rubratoxin B(RB)在体外诱导人绒癌细胞株(BeWo)发生凋亡,并探讨其与抑制凋亡基因Bcl-2mRNA表达的关系。方法:利用MTT、HE染色、annexinV-FITC/PI流式细胞技术定性及定量分析经RB处理后BeWo细胞活细胞及凋亡细胞的变化情况;利用逆转录聚合酶链式反应(RT-PCR)技术检测抑制凋亡基因Bcl-2与RB诱导的BeWo细胞凋亡的关系。结果:RB诱导BeWo细胞凋亡的数量与药物浓度及用药时间明显相关。RT-PCR结果显示Bcl-2mRNA的表达随着凋亡细胞的增加而减少。结论:RB能够诱导BeWo细胞发生凋亡,Bcl-2mRNA表达的下调是RB诱导BeWo细胞凋亡的重要机制。

A cascade of redox reactions in the biosynthesis of the protein phosphatase-2A inhibitor rubratoxin A

Subject Code:H30 With the support by the National Natural Science Foundation of China,a collaborative study by the research groups led by Prof.Hu Youcai(胡友财),Prof.Yu Shishan(庾石山)and Prof.Tang Yi(唐奕)from the State Key Laboratory of Bioactive Substance and Function of Natural Medicines,Institute