Lactobacillus pentosus S-PT84 and Rubus suavissimus leaf extract suppress lipopolysaccharide-induced gut permeability and egg allergen uptake

Increased gut permeability facilitates the uptake of food allergens into the bloodstream and triggers allergenic reactions.The present study aimed to evaluate the effect of Lactobacillus pentosus S-PT84(S-PT84)and Rubus suavissimus leaf extract(RSLE)against egg ovomucoid(OVM)-uptake in lipopolysaccharide(LPS)-induced increased gut-permeability mice model of food allergy.Six-eight weeks old,female C57BL6 mice were continuously fed with LPS(300μg/kg BW),for 3 months to increase gut permeability.Reduction in the expression of sealing claudin-4,increase in the expression of pore-forming claudin-2,and increase in D-mannitol absorption into the blood plasma in the LPS treated groups suggested the increase in gut permeability after LPS treatment.The oral administration of major egg allergen,OVM,after LPS intervention,significantly increased the plasma mast cell protease-1 and OVMspecific IgE compared to the negative control group.These results indicated that continuous LPS intervention developed OVM-induced food allergy.However,both the treatment of S-PT84 and RSLE suppressed the claudin-2 expression and the gut permeability induced by LPS.Furthermore,S-PT84 and RSLE treatment also reduced the plasma mast cell protease-1 and OVM-specific IgE,indicating the potential beneficial effect against LPS intervention developed OVM-induced food allergy.These findings suggest that S-PT84 and RSLE ameliorated LPS induced gut permeability and food allergic reactions.

不同药剂防治广西甜茶毛毡病田间药效试验

对广西甜茶(Rubus suavissimus S.Lee)毛毡病防治药剂进行筛选研究。结果表明,5.6%阿维·哒螨灵乳油1000倍稀释液、22%螺螨酯悬浮剂3000倍稀释液,对甜茶毛毡病具有较好的防治效果,防治效果分别为80.9%、83.3%。