Objective: To investigate the inhibitory effects against dengue virus serotype 2(DENV-2) by five different fractions(extracted by methanol, ethanol, benzene, chloroform and n-hexane) of Rumex dentatus, Commelina bengh...Objective: To investigate the inhibitory effects against dengue virus serotype 2(DENV-2) by five different fractions(extracted by methanol, ethanol, benzene, chloroform and n-hexane) of Rumex dentatus, Commelina benghalensis, Ajuga bracteosa and Ziziphus mauritiana, as well as their constituents(gallic acid, emodin, and isovanillic acid). Methods: All the samples were tested for cytotoxicity on baby hamster kidney cells by MTT assay and for anti-DENV-2 activity by plaque reduction neutralization assay using two DENV-2 doses(45 and 90 plaqueforming units or PFU). Results: All the samples except isovanillic acid exhibited significant prophylactic effects against DENV-2 infectivity(without cytotoxicity) when administered to cells before infection, but were not effective when given 6 h post-infection. The methanol extract of Rumex dentatus demonstrated the highest antiviral efficacy by inhibiting DENV-2 replication, with IC_(50) of 0.154 μg/mL and 0.234 μg/mL, when added before infection with 45 and 90 PFU of virus, respectively. Gallic acid also exhibited significant antiviral effects by prophylactic treatment prior to virus adsorption on cells, with IC_(50) of 0.191 μg/mL and 0.522 μg/mL at 45 and 90 PFU of DENV-2 infection, respectively. Conclusions: The highly potent activities of the extracts and constituent compounds of these plants against DENV-2 infectivity highlight their potential as targets for further research to identify novel antiviral agents against dengue.展开更多
Rumex L.,a genus in Polygonaceae family with about 200 species,is growing widely around the world.Some Rumex species,called"sorrel"or"dock",have been used as food application and treatment of skin ...Rumex L.,a genus in Polygonaceae family with about 200 species,is growing widely around the world.Some Rumex species,called"sorrel"or"dock",have been used as food application and treatment of skin diseases and hemostasis after trauma by the local people of its growing areas for centuries.To date,29 Rumex species have been studied to contain about 268 substances,including anthraquinones,flavonoids,naphthalenes,stilbenes,diterpene alkaloids,terpenes,lignans,and tannins.Crude extract of Rumex spp.and the pure isolates displayed various bioactivities,such as antibacterial,anti-inflammatory,antitumor,antioxidant,cardiovascular protection and antiaging activities.Rumex species have important potential to become a clinical medicinal source in future.This review covers research articles from 1900 to 2022,fetched from SciFinder,Web of Science,ResearchGate,CNKI and Google Scholar,using“Rumex”as a search term("all fields")with no specific time frame set for the search.Thirty-five Rumex species were selected and summarized on their geographical distribution,edible parts,traditional uses,chemical research and pharmacological properties.展开更多
Objective:To assess in-vitro antioxidant activity of different fraction and perform high performance thin layer chromatography fingerprint analysis of most active fraction of Rumex vesicarius L.(R.vesicarius).Methods:...Objective:To assess in-vitro antioxidant activity of different fraction and perform high performance thin layer chromatography fingerprint analysis of most active fraction of Rumex vesicarius L.(R.vesicarius).Methods:In the present study,acetone,ethyl acetate,n-butanol,and methanol extracts of R.vesicarius were evaluated for radical scavenging activity by studying the inhibition of the level of lipid peroxidation induced by Fe(++)/ascorbate,DNA sugar damage,scavenging of hydrogen peroxide,diphenylphosphine DPPH radical scavenging activity,total phenolic content,total flavonoids content and total proanthocyanidin.High performance thin layer chromatography finger print profiling of R.vesicarius L.was also done.Results:Lipid peroxidation induced by the iron/ascorbate system,hydrogen peroxide,diphenylphosphine and DNA sugar damage were inhibited by the addition of different extract of R.vesicarius.Among them,methanolic extract showed maximum efficacy.The methanolic extract showed the highest total phenolic,total flavonoids and total proanthocyanidin contents.Conclusions:The results suggest that the extracts can be a vital source of phytochemical antioxidants.展开更多
A new seco-anthraquinone,crispuside A(1),and three new 3,4-dihydronaphthalen-1(2H)-ones,napthalenones A-C(2-4),were isolated from the roots of Rumex crispus L.,along with 10 known anthraquinones(6-14)and naph-thalenon...A new seco-anthraquinone,crispuside A(1),and three new 3,4-dihydronaphthalen-1(2H)-ones,napthalenones A-C(2-4),were isolated from the roots of Rumex crispus L.,along with 10 known anthraquinones(6-14)and naph-thalenone(5).Their structures were fully determined by extensive spectroscopic analyses,including ECD,and X-ray crystallography in case of compound 5,whose absolute configuration was determined for the first time.The isolates 1,6-14 were evaluated for their anti-inflammatory and anti-fungal activity against three skin fungi,e.g.,Epidermophyton floccosum,Trichophyton rubrum,and Microsporum gypseum.Most of the isolates showed weak anti-fungal and anti-inflammatory activity.Only compound 9 exhibited obvious anti-fungal activity against E.floccosum(MIC_(50)=2.467±0.03μM)and M.gypseum(MIC_(50)=4.673±0.077μM),while the MIC_(50)values of the positive control terbinafine were 1.287±0.012 and 0.077±0.00258μM,respectively.The results indicated that simple emodin type anthraquinone is more potential against skin fungi than its oxyglucosyl,C-glucosyl and glycosylated seco analogues.展开更多
Unconventional vegetables, in general, are plants that have been largely consumed by the population at some point and, because of changes in eating behavior, now present reduced economic and social expression and have...Unconventional vegetables, in general, are plants that have been largely consumed by the population at some point and, because of changes in eating behavior, now present reduced economic and social expression and have lost ground to other vegetables. The objectives of this study were to perform phytochemical screening of the ethanol extracts of Rumex acetosa L., Tropaeolum majus L., Solanum muricatum, Stachys byzantina K. Koch and Solanum betaceum Cav. and evaluate their antioxidant potentials via the methods involving scavaging of the DPPH free radical and the ABTS radical, phosphomolybdenum and reducing power. In phytochemical screening, five species of unconventional vegetables tested positive for tannins;for sesquiterpene, lactones and other lactones. These tests were positive for Tropaeolum majus L. and Rumex acetosa L., Solanum betaceum Cav. and Solanum muricatum tested negative for steroids. Only Solanum betaceum Cav. gave positive tests for flavonoids. Among the five plant species studied, Stachys byzantina K. Koch presented the greatest antioxidant potential in all the methods evaluated.展开更多
基金support of the National University of SingaporeQuaid-i-Azam University
文摘Objective: To investigate the inhibitory effects against dengue virus serotype 2(DENV-2) by five different fractions(extracted by methanol, ethanol, benzene, chloroform and n-hexane) of Rumex dentatus, Commelina benghalensis, Ajuga bracteosa and Ziziphus mauritiana, as well as their constituents(gallic acid, emodin, and isovanillic acid). Methods: All the samples were tested for cytotoxicity on baby hamster kidney cells by MTT assay and for anti-DENV-2 activity by plaque reduction neutralization assay using two DENV-2 doses(45 and 90 plaqueforming units or PFU). Results: All the samples except isovanillic acid exhibited significant prophylactic effects against DENV-2 infectivity(without cytotoxicity) when administered to cells before infection, but were not effective when given 6 h post-infection. The methanol extract of Rumex dentatus demonstrated the highest antiviral efficacy by inhibiting DENV-2 replication, with IC_(50) of 0.154 μg/mL and 0.234 μg/mL, when added before infection with 45 and 90 PFU of virus, respectively. Gallic acid also exhibited significant antiviral effects by prophylactic treatment prior to virus adsorption on cells, with IC_(50) of 0.191 μg/mL and 0.522 μg/mL at 45 and 90 PFU of DENV-2 infection, respectively. Conclusions: The highly potent activities of the extracts and constituent compounds of these plants against DENV-2 infectivity highlight their potential as targets for further research to identify novel antiviral agents against dengue.
基金supported by the Ministry of Science and Technology,China (2021YFE0103600) for International Scientific and Technological Innovative Cooperation between Governments。
文摘Rumex L.,a genus in Polygonaceae family with about 200 species,is growing widely around the world.Some Rumex species,called"sorrel"or"dock",have been used as food application and treatment of skin diseases and hemostasis after trauma by the local people of its growing areas for centuries.To date,29 Rumex species have been studied to contain about 268 substances,including anthraquinones,flavonoids,naphthalenes,stilbenes,diterpene alkaloids,terpenes,lignans,and tannins.Crude extract of Rumex spp.and the pure isolates displayed various bioactivities,such as antibacterial,anti-inflammatory,antitumor,antioxidant,cardiovascular protection and antiaging activities.Rumex species have important potential to become a clinical medicinal source in future.This review covers research articles from 1900 to 2022,fetched from SciFinder,Web of Science,ResearchGate,CNKI and Google Scholar,using“Rumex”as a search term("all fields")with no specific time frame set for the search.Thirty-five Rumex species were selected and summarized on their geographical distribution,edible parts,traditional uses,chemical research and pharmacological properties.
基金Supported by Deanship of Scientific Research,Salman binAbdulaziz University,Al-Kharj Saudi Arabia(Grant No.4/1432)
文摘Objective:To assess in-vitro antioxidant activity of different fraction and perform high performance thin layer chromatography fingerprint analysis of most active fraction of Rumex vesicarius L.(R.vesicarius).Methods:In the present study,acetone,ethyl acetate,n-butanol,and methanol extracts of R.vesicarius were evaluated for radical scavenging activity by studying the inhibition of the level of lipid peroxidation induced by Fe(++)/ascorbate,DNA sugar damage,scavenging of hydrogen peroxide,diphenylphosphine DPPH radical scavenging activity,total phenolic content,total flavonoids content and total proanthocyanidin.High performance thin layer chromatography finger print profiling of R.vesicarius L.was also done.Results:Lipid peroxidation induced by the iron/ascorbate system,hydrogen peroxide,diphenylphosphine and DNA sugar damage were inhibited by the addition of different extract of R.vesicarius.Among them,methanolic extract showed maximum efficacy.The methanolic extract showed the highest total phenolic,total flavonoids and total proanthocyanidin contents.Conclusions:The results suggest that the extracts can be a vital source of phytochemical antioxidants.
基金supported by Ministry of Science and Technology of the People’s Republic of China,2021YFE0103600.
文摘A new seco-anthraquinone,crispuside A(1),and three new 3,4-dihydronaphthalen-1(2H)-ones,napthalenones A-C(2-4),were isolated from the roots of Rumex crispus L.,along with 10 known anthraquinones(6-14)and naph-thalenone(5).Their structures were fully determined by extensive spectroscopic analyses,including ECD,and X-ray crystallography in case of compound 5,whose absolute configuration was determined for the first time.The isolates 1,6-14 were evaluated for their anti-inflammatory and anti-fungal activity against three skin fungi,e.g.,Epidermophyton floccosum,Trichophyton rubrum,and Microsporum gypseum.Most of the isolates showed weak anti-fungal and anti-inflammatory activity.Only compound 9 exhibited obvious anti-fungal activity against E.floccosum(MIC_(50)=2.467±0.03μM)and M.gypseum(MIC_(50)=4.673±0.077μM),while the MIC_(50)values of the positive control terbinafine were 1.287±0.012 and 0.077±0.00258μM,respectively.The results indicated that simple emodin type anthraquinone is more potential against skin fungi than its oxyglucosyl,C-glucosyl and glycosylated seco analogues.
基金The authors thank the Conselho Nacional de Desenvolvimento Científico e Tecnológico(CNPq)the Coordenacao de Aperfeiçoamento de Pessoal de Nível Superior(CAPES) the Fundacao de AmparoàPesquisa do Estado de Minas Gerais(FAPEMIG)for financial support,a scholarship and a PVNS fellowship(D.L.Nelson).
文摘Unconventional vegetables, in general, are plants that have been largely consumed by the population at some point and, because of changes in eating behavior, now present reduced economic and social expression and have lost ground to other vegetables. The objectives of this study were to perform phytochemical screening of the ethanol extracts of Rumex acetosa L., Tropaeolum majus L., Solanum muricatum, Stachys byzantina K. Koch and Solanum betaceum Cav. and evaluate their antioxidant potentials via the methods involving scavaging of the DPPH free radical and the ABTS radical, phosphomolybdenum and reducing power. In phytochemical screening, five species of unconventional vegetables tested positive for tannins;for sesquiterpene, lactones and other lactones. These tests were positive for Tropaeolum majus L. and Rumex acetosa L., Solanum betaceum Cav. and Solanum muricatum tested negative for steroids. Only Solanum betaceum Cav. gave positive tests for flavonoids. Among the five plant species studied, Stachys byzantina K. Koch presented the greatest antioxidant potential in all the methods evaluated.
