Objective:To investigate the role of Runx3 protein and TGF-β<sub>1</sub> in the pathogenesis of irritable bowel syndrome（IBS）,as well as the correlation of these two proteins.Methods:Colonic tissue was ...Objective:To investigate the role of Runx3 protein and TGF-β<sub>1</sub> in the pathogenesis of irritable bowel syndrome（IBS）,as well as the correlation of these two proteins.Methods:Colonic tissue was collected from patients with IBS and normal persons.The colonic expression of Runx3 protein and TGF-β<sub>1</sub> was detected with immunohislochemistry method.Semi-quantitative analysis was used to evaluate the staining degree of these two proteins.Results:Compared with their counterparts,patients with IBS did not show any changes in the colonic expression of Runx3 protein and TGF-β<sub>1</sub>（P】0.05）.Interestingly,there was a significant correlation between Runx3 protein and TGF-β<sub>1</sub> in patients with IBS（P【0.05）.Conclusions:The role of Runx3 protein and TGF-β<sub>1</sub> in the pathogenesis of IBS remains to be further studied.展开更多
Objective:To investigate the expression of Runx3 and TGF-β<sub>1</sub> protein in the colon from rats with irritable bowel syndrome（IBS）.Methods:Rat model for IBS was established by intracolonic instill...Objective:To investigate the expression of Runx3 and TGF-β<sub>1</sub> protein in the colon from rats with irritable bowel syndrome（IBS）.Methods:Rat model for IBS was established by intracolonic instillation with acetic acid and restraint stress methods,which was confirmed by determinating the visceral sensitivity of the animals,including abdominal withdrawal reflex （AWR） score and the electronic behavior of the abdomen wall.The rats were randomly assigned into three groups:IBS,group（restraint stress,n=25）;IBS<sub>2</sub> group（both instillation with acetic acid and restraint stress,n=25） and Control group（n=16）.The colonic tissue samples were collected for histological study and the expression of Runx3 and TGF-β<sub>1</sub> proteins were detected by immunohistochemistry.Meanwhile,the relationship of these two proteins was calculated. Results:Visceral hypersensitivity（AWR and abdominal electrical activity） was significantly enhanced in IBS,and IBS<sub>2</sub> groups than other groups.The colon tissue in all groups did not show any signs of inflammation.Furthermore,the expression of Runx3 and TGF-β<sub>1</sub> protein in the colon from all groups show no significant difference（P】0.05）,with no remarkable relevancy between each other（P】0.05）.Conclusions:The rat model for IBS was successfully established. We did not find any significant changes in the expression of Runx3 and TGF-β<sub>1</sub> protein in the colon tissue from IBS rats,suggesting that the quantitative changes may be not the way by which Runx3 and TGF-β<sub>1</sub> protein play their roles in IBS.The accurate roles of Runx3 and TGF-β<sub>1</sub> proteins in the pathogenesis of IBS remains to be further studied.展开更多
基金Supported by Natural Scienee Foundation of Hainan Province(No 30855)
文摘Objective:To investigate the role of Runx3 protein and TGF-β<sub>1</sub> in the pathogenesis of irritable bowel syndrome（IBS）,as well as the correlation of these two proteins.Methods:Colonic tissue was collected from patients with IBS and normal persons.The colonic expression of Runx3 protein and TGF-β<sub>1</sub> was detected with immunohislochemistry method.Semi-quantitative analysis was used to evaluate the staining degree of these two proteins.Results:Compared with their counterparts,patients with IBS did not show any changes in the colonic expression of Runx3 protein and TGF-β<sub>1</sub>（P】0.05）.Interestingly,there was a significant correlation between Runx3 protein and TGF-β<sub>1</sub> in patients with IBS（P【0.05）.Conclusions:The role of Runx3 protein and TGF-β<sub>1</sub> in the pathogenesis of IBS remains to be further studied.
基金Supported by Natural Science Fundation of Hainan Province 2008(No 30855)
文摘Objective:To investigate the expression of Runx3 and TGF-β<sub>1</sub> protein in the colon from rats with irritable bowel syndrome（IBS）.Methods:Rat model for IBS was established by intracolonic instillation with acetic acid and restraint stress methods,which was confirmed by determinating the visceral sensitivity of the animals,including abdominal withdrawal reflex （AWR） score and the electronic behavior of the abdomen wall.The rats were randomly assigned into three groups:IBS,group（restraint stress,n=25）;IBS<sub>2</sub> group（both instillation with acetic acid and restraint stress,n=25） and Control group（n=16）.The colonic tissue samples were collected for histological study and the expression of Runx3 and TGF-β<sub>1</sub> proteins were detected by immunohistochemistry.Meanwhile,the relationship of these two proteins was calculated. Results:Visceral hypersensitivity（AWR and abdominal electrical activity） was significantly enhanced in IBS,and IBS<sub>2</sub> groups than other groups.The colon tissue in all groups did not show any signs of inflammation.Furthermore,the expression of Runx3 and TGF-β<sub>1</sub> protein in the colon from all groups show no significant difference（P】0.05）,with no remarkable relevancy between each other（P】0.05）.Conclusions:The rat model for IBS was successfully established. We did not find any significant changes in the expression of Runx3 and TGF-β<sub>1</sub> protein in the colon tissue from IBS rats,suggesting that the quantitative changes may be not the way by which Runx3 and TGF-β<sub>1</sub> protein play their roles in IBS.The accurate roles of Runx3 and TGF-β<sub>1</sub> proteins in the pathogenesis of IBS remains to be further studied.
