We had previously identified a novel protein termed Rwddl whose expression in thymus is decreased in aged or oxidatively stressed mice. In the present study, we found that Rwddl expressed in both prokaryotic and eukar...We had previously identified a novel protein termed Rwddl whose expression in thymus is decreased in aged or oxidatively stressed mice. In the present study, we found that Rwddl expressed in both prokaryotic and eukaryotic ceils showed a slower migration rate on SDS-PAGE gel. In addition, Rwddl was more sensitive to proteinase proteolysis. Furthermore, being a highly acidic protein which contains an RWD domain, Rwddl shared a high level of sequence similarity with Gir2, a member of the intrinsically unstructured protein （IUP）. These findings suggest that Rwddl is a novel member of the IUP family.展开更多
By using DDRT-PCR and EST segment ligation, a novel mouse thymus involution related gene Rwddl was identified. The reading frame encoded a protein of 243 amino acid residues which contained an RWD domain at the N term...By using DDRT-PCR and EST segment ligation, a novel mouse thymus involution related gene Rwddl was identified. The reading frame encoded a protein of 243 amino acid residues which contained an RWD domain at the N terminus. Rwddl expression in thymus was decreased in aged and oxidatively stressed mice. It was found to be expressed extensively in thymocytes and thymic epithelial cells. The expression level of Rwddl could affect the transactivation activity of androgen receptor （AR） in transiently transfected thymic epithelial cells. However, no direct interaction could be detected by fluorescence resonance energy transfer （FRET） analysis. In conclusion, Rwddl is a thymus involution related protein that may indirectly affect AR signaling pathway. Cellular ＆ Molecular Immunology. 2008;5（4）：279-285.展开更多
基金a grant from theJapan Institute for the Control of Aging (JaICA 2000-2003)a fund from the National Natural Science Foundation of China (No. 30471589) to Dr. Wei He.
文摘We had previously identified a novel protein termed Rwddl whose expression in thymus is decreased in aged or oxidatively stressed mice. In the present study, we found that Rwddl expressed in both prokaryotic and eukaryotic ceils showed a slower migration rate on SDS-PAGE gel. In addition, Rwddl was more sensitive to proteinase proteolysis. Furthermore, being a highly acidic protein which contains an RWD domain, Rwddl shared a high level of sequence similarity with Gir2, a member of the intrinsically unstructured protein （IUP）. These findings suggest that Rwddl is a novel member of the IUP family.
基金a grant (JaICA 2000-2003) from the Japan Institute for the Control of Agingthe funds from the National Natural Science Foundation of China (No. 30471589) to Dr. Wei He.
文摘By using DDRT-PCR and EST segment ligation, a novel mouse thymus involution related gene Rwddl was identified. The reading frame encoded a protein of 243 amino acid residues which contained an RWD domain at the N terminus. Rwddl expression in thymus was decreased in aged and oxidatively stressed mice. It was found to be expressed extensively in thymocytes and thymic epithelial cells. The expression level of Rwddl could affect the transactivation activity of androgen receptor （AR） in transiently transfected thymic epithelial cells. However, no direct interaction could be detected by fluorescence resonance energy transfer （FRET） analysis. In conclusion, Rwddl is a thymus involution related protein that may indirectly affect AR signaling pathway. Cellular ＆ Molecular Immunology. 2008;5（4）：279-285.
