Cohesin is an evolutionary conserved complex that controls chromosome segregation during mitosis. Here we show that, in response to DNA damage, Saccharomyces cerevisiae Scc3, one of the major regulatory subunits of th...Cohesin is an evolutionary conserved complex that controls chromosome segregation during mitosis. Here we show that, in response to DNA damage, Saccharomyces cerevisiae Scc3, one of the major regulatory subunits of the Smc1-Smc3-Scc1 cohesin ring, is phosphorylated on S/T-Q residues. This event depended on the Mec1 checkpoint kinase as well as on cell cycle arrest triggered by the DNA damage checkpoint network. This phosphorylation event also took place during mitosis of an unperturbed cell cycle. The present finding that S. cerevisiae Scc3 is phosphorylated during mitosis represents a potentially important new regulatory step in cohesin’s mitotic functions.展开更多
Based on the waveform data of 5,076 local earthquakes recorded at 25 stations in Xinjiang during the period from 2009 to 2014 and the observation reports provided by the Xinjiang Digital Seismic Network,a data set of ...Based on the waveform data of 5,076 local earthquakes recorded at 25 stations in Xinjiang during the period from 2009 to 2014 and the observation reports provided by the Xinjiang Digital Seismic Network,a data set of 19,140 attenuation factors t*is obtained by fitting the high-frequency attenuation of S-wave spectra with a genetic algorithm. The spatial distribution of Q_S is determined by inverting the t*data with seismic tomography. The results show that the average Q0 in eastern Tianshan is 520,and there is a significant correlation between the Q_S value distribution or attenuation characteristics it disclosed and the surface structure of the study area. The Q_S value is lower in the intersection area of the mountain basin which is located on the north and south sides of the Tianshan Mountains,and the high Q_S distribution is more concentrated inside the Tianshan orogenic belt. The M≥6. 0 earthquakes have been basically located in the Low-Q_S region since 1900. 24 high heat flow points in eastern Tianshan are located at the north and south of Tianshan Mountains where low Q_S exists,indicating a negative correlation. In addition,there is a positive correlation between the velocity structure and the attenuation structure in the study area,which reflects the consistency of the 2-D attenuation structure with the velocity structure and the two-dimensional density structure.展开更多
煤矿区深部含水层具有大埋深、高水压等特点,是深部煤层开采水害防范的主要对象。在分析深部矿井水害特征基础上,探讨了深部矿井地应力对裂隙水介质的力学作用机制,阐述了深部矿井裂隙水介质呈现“高承压、弱富水”的力学原因;基于古岩...煤矿区深部含水层具有大埋深、高水压等特点,是深部煤层开采水害防范的主要对象。在分析深部矿井水害特征基础上,探讨了深部矿井地应力对裂隙水介质的力学作用机制,阐述了深部矿井裂隙水介质呈现“高承压、弱富水”的力学原因;基于古岩溶成因演化理论,结合水文地质勘探和野外调查,分析探讨了华北型煤田奥陶纪石灰岩顶部“古风化壳”的岩溶充填结构特征,并将奥陶纪石灰岩顶部“古风化壳”划分为“隔水充填带、弱隔(透)水充填带、富(透)水带”3种岩溶充填结构类型,给出了各类型确定的关键指标和阈值,明确了奥陶纪灰岩顶部存在隔水层的理论依据。将深部矿井煤层开采底板突水划分为“完整底板突水模式”和“集中破碎带底板突水模式”,分析了深部煤层开采底板含水层高水压作用下沿用传统的突水系数法( T s 法)评价底板突水危险性的局限性,引入隔水层厚度( M )和含水层钻孔单位涌水量( q )2个指标,结合突水实例分析,提出了“修正的突水系数法”( T s -M-q 法,适用于完整底板突水模式);在实验和实例分析基础上,提出“渗-流转换”突水评价法(适用于集中破碎带底板突水模式)。最后,基于深部矿井水害特征及突水模式,结合深部含水介质赋存、构造发育特征和地应力场方向,阐述了深部矿井水害精准查治一体化勘探关键技术。展开更多
文摘Cohesin is an evolutionary conserved complex that controls chromosome segregation during mitosis. Here we show that, in response to DNA damage, Saccharomyces cerevisiae Scc3, one of the major regulatory subunits of the Smc1-Smc3-Scc1 cohesin ring, is phosphorylated on S/T-Q residues. This event depended on the Mec1 checkpoint kinase as well as on cell cycle arrest triggered by the DNA damage checkpoint network. This phosphorylation event also took place during mitosis of an unperturbed cell cycle. The present finding that S. cerevisiae Scc3 is phosphorylated during mitosis represents a potentially important new regulatory step in cohesin’s mitotic functions.
基金jointly funded by the Contract Oriented Work Task for Seismic Situation in 2017(2017010104)Science for Earthquake Resilience(XH17041Y)Fund of Earthquake Agency of Xinjiang Uygur Autonomous Region(201401)
文摘Based on the waveform data of 5,076 local earthquakes recorded at 25 stations in Xinjiang during the period from 2009 to 2014 and the observation reports provided by the Xinjiang Digital Seismic Network,a data set of 19,140 attenuation factors t*is obtained by fitting the high-frequency attenuation of S-wave spectra with a genetic algorithm. The spatial distribution of Q_S is determined by inverting the t*data with seismic tomography. The results show that the average Q0 in eastern Tianshan is 520,and there is a significant correlation between the Q_S value distribution or attenuation characteristics it disclosed and the surface structure of the study area. The Q_S value is lower in the intersection area of the mountain basin which is located on the north and south sides of the Tianshan Mountains,and the high Q_S distribution is more concentrated inside the Tianshan orogenic belt. The M≥6. 0 earthquakes have been basically located in the Low-Q_S region since 1900. 24 high heat flow points in eastern Tianshan are located at the north and south of Tianshan Mountains where low Q_S exists,indicating a negative correlation. In addition,there is a positive correlation between the velocity structure and the attenuation structure in the study area,which reflects the consistency of the 2-D attenuation structure with the velocity structure and the two-dimensional density structure.
文摘煤矿区深部含水层具有大埋深、高水压等特点,是深部煤层开采水害防范的主要对象。在分析深部矿井水害特征基础上,探讨了深部矿井地应力对裂隙水介质的力学作用机制,阐述了深部矿井裂隙水介质呈现“高承压、弱富水”的力学原因;基于古岩溶成因演化理论,结合水文地质勘探和野外调查,分析探讨了华北型煤田奥陶纪石灰岩顶部“古风化壳”的岩溶充填结构特征,并将奥陶纪石灰岩顶部“古风化壳”划分为“隔水充填带、弱隔(透)水充填带、富(透)水带”3种岩溶充填结构类型,给出了各类型确定的关键指标和阈值,明确了奥陶纪灰岩顶部存在隔水层的理论依据。将深部矿井煤层开采底板突水划分为“完整底板突水模式”和“集中破碎带底板突水模式”,分析了深部煤层开采底板含水层高水压作用下沿用传统的突水系数法( T s 法)评价底板突水危险性的局限性,引入隔水层厚度( M )和含水层钻孔单位涌水量( q )2个指标,结合突水实例分析,提出了“修正的突水系数法”( T s -M-q 法,适用于完整底板突水模式);在实验和实例分析基础上,提出“渗-流转换”突水评价法(适用于集中破碎带底板突水模式)。最后,基于深部矿井水害特征及突水模式,结合深部含水介质赋存、构造发育特征和地应力场方向,阐述了深部矿井水害精准查治一体化勘探关键技术。
