Effects of Maixuekang Capsules Combined with Edaravone on Serum MMP-9, S-100β Protein Levels and Neurological Functions in Patients with Hemorrhagic Cerebral Infarction

OBJECTIVE: To investigate the effects of Maixuekang Capsules combined with edaravone on serum matrix metalloproteinase-9(MMP-9), S-100β protein levels and neurological functions in patients with hemorrhagic cerebral infarction. METHOSDS: A total of 76 patients with hemorrhagic cerebral infarction treated in the First Affiliated Hospital of Henan University of Science and Technology from January 2017 to May 2018 were selected and were randomly divided into treatment group and control group, with 38 patients in each group. The control group was given edaravone, and the treatment group was given Maixuekang Capsules on the basis of the control group. The clinical efficacy, serum MMP-9 and S-100β protein levels, neurological function recovery, activity of daily living and incidence rate of adverse reactions were compared between the 2 groups. RESULTS: The total effective rate of the treatment group was 92.11%, which was higher than 71.05% of the control group(P < 0.05); the National Institutes of Health Stroke Scale(NIHSS) score in the 2 groups decreased(P < 0.05), and the Activity of Daily Living Scale(ADL) score increased(P < 0.05), the improvement of the above 2 scores in the treatment group were better than those in the control group(P < 0.05); the level of MMP-9 was gradually decreasing in the 2 groups, on the 7th day, 14 th day after treatment, and the levels of MMP-9 decreased significantly(P < 0.05), and the treatment group was lower than the control group at all time points(P < 0.05); on the 3rd day after treatment, the levels of S-100β protein in the 2 groups increased significantly(P < 0.05); on the 7th day, 14 th day after treatment, the levels of S-100β protein in the two groups decreased significantly(P < 0.05), and the treatment group was significantly lower than the control group(P < 0.05); there was no significant difference in incidence rate of adverse reactions between 7.89% in the control group and 5.26% in the treatment group(P > 0.05). CONCLUSION: The combination of Maixuekang Capsules and edaravone is effective in treating hemorrhagic cerebral infarction, and it can significantly improve neurological function defect and daily living ability, reduce serum MMP-9 and S-100β protein levels, and has higher safety.

甲泼尼龙对深低温脑缺血再灌注大鼠血清S-100β蛋白的影响

目的:通过比较血清S100β蛋白变化来观察甲泼尼龙对深低温脑缺血再灌注大鼠脑损害的作用。方法:120只SD大鼠随机分为三组。假手术组8只;模型组、甲泼尼龙组各56只,分为缺血再灌注后2、6、12h和1、2、3、7d共七个小组,每小组8只,建立深低温鼠脑缺血再灌注模型后,用酶联免疫检测技术定量检测不同时相点血清S100β蛋白水平。结果:假手术组血清S100β为(0.24±0.02)μg/L,模型组全部七个小组和甲泼尼龙处理组6h～3d血清S100β蛋白水平均比假手术组高(P<0.05),甲泼尼龙组6h～3d血清S100β蛋白水平比模型组低(P<0.01)。结论:甲泼尼龙对大鼠深低温脑缺血再灌注损伤有干预治疗作用。

毒鼠强中毒患者血清和脑脊液NSE、S-100β、CK-BB的变化

目的探讨急性毒鼠强中毒患者血清和脑脊液(CSF)中神经元特异性烯醇化酶(NSE)、S-100β蛋白(S-100β)、磷酸肌酸激酶脑型同工酶(CK-BB)水平变化及其临床意义。方法检测39例急性毒鼠强中毒患者血液和CSF中NSE、S-100β、CK-BB水平的变化,与20例对照组比较分析,并对中毒组血清和CSF中NSE、S-100β、CK-BB水平进行相关分析。结果中毒各组血清和CSF中NSE、S-100β、CK-BB的含量均显著高于对照组,且病情越严重其升高越明显,差异有统计学意义(P<0.01或P<0.05),并且血液和脑脊液中NSE、S-100β、CK-BB水平的变化具有较好相关性。结论血液和脑脊液中NSE、S-100β和CK-BB水平的变化,可作为评估急性毒鼠强中毒脑组织损伤及其严重程度的临床指标。

丙种球蛋白对重症手足口病患儿细胞因子、hs-cTn、NSE和S-100β蛋白的影响

目的探讨丙种球蛋白对重症手足口病(HFMD)患儿炎症细胞因子、hs-cTn、NSE和S-100β蛋白的影响。方法将88例重症HFMD患儿随机分为观察组(44例)和对照组(44例)。对照组给予常规方案治疗,观察组在常规治疗的基础上给予丙种球蛋白。评估两组的疗效,检测两组患儿治疗前后的白介素-6(IL-6)、IL-8、IL-10、干扰素-γ(INF-γ)、肿瘤坏死因子-α(TNF-α)、高敏心肌肌钙蛋白(hs-cTn)、神经元特异性烯醇化酶(NSE)和S-100β蛋白的水平。结果观察组的总有效率明显高于对照组(P<0.05)。两组治疗后的IL-6、IL-8、INF-γ、TNF-α、hs-cTn、NSE和S-100β蛋白水平均明显低于治疗前,IL-10水平明显高于治疗前,且以观察组更加明显(P<0.05)。结论丙种球蛋白可明显提高重症HFMD的疗效和调节免疫紊乱,并可明显降低hs-cTn、NSE和S-100β蛋白水平。

心肺复苏后患者S-100β蛋白的动态变化与预后关系的研究

目的:探讨心肺复苏后患者血清S-100β蛋白的动态变化与预后关系。方法:采用酶联免疫吸附试验双抗体夹心法对30例心肺复苏后患者1 h、1 d、3 d、5 d、7 d的血清S-100β蛋白含量进行动态监测。对照组为健康者20例,入院后采血1次。将两组结果进行统计学分析比较。结果:(1)心肺复苏后患者1 h至1 d的血清S-100β蛋白的含量高于对照组(P<0.01);但第3d、第5d、第7d分别与对照组间差异无显著性(P>0.05)。(2)心肺复苏患者血清平均S-100β蛋白含量的峰值出现在心肺复苏后的1 h至1 d。(3)心肺复苏后患者1 h至1 d的血清S-100β蛋白的含量与病情严重程度相关(r=0.688,P=0.001)。结论:心肺复苏后患者血清S-100β蛋白的含量可作为病情严重程度的一个重要指标。

甲基强的松龙对深低温脑缺血再灌注大鼠脑组织S-100β蛋白表达的影响

目的观察甲基强的松龙(MP)对深低温脑缺血再灌注大鼠脑组织S-100β蛋白表达的影响。方法将SD大鼠随机分为假手术组、模型组和MP组,应用SABC技术检测脑组织S-100β蛋白表达。结果假手术组脑组织S-100β蛋白呈基础水平表达;MP组S-100β蛋白表达量与模型组比较显著降低。结论MP可能通过抑制S-100β的过量表达,对深低温缺血再灌注脑损害发挥保护作用。

手足口病并发脑干脑炎患儿血清hs-CRP、S-100B蛋白检测的临床意义

C-反应蛋白（C-reactive protein,CRP）是一种急性时相蛋白,在人体发生感染、创伤等情况时会迅速上升,经治疗后又会迅速下降。对观察病情的变化有十分重要的临床价值。S-100B蛋白检测对脑实质性损伤有十分重要的临床价值。

APPLICATION OF HMB－45 MONOCLONAL ANTIBODY AND S100 PROTEIN IN THE IMMUNOHISTOCHEMICAL DIAGNOSIS OF MELANOMA

We tested a variety of fixed embedded sections of malignant tumors with HMB-45 MoAband S-100 polyclonal antibody.The results showed that RMB-45 was a highly sensitive and specificantibody for recongnizing melanoma on fixed paraffin-embedded tissue sections, it reacted with 96.6percent of melanomas tested(all primary and 6 of 7 metastatic lesions)Both pigmented and nonpigmeated melanomas were recongnized.Malignant tumors of epithelial,lymphoid and mesenchymal origin were all negative.Although antibody to S-100 protien quite sensitive,it was not melanome-specific and it reached with all melanomas including the one metastatic melanoma that did not react withHMB-45,it we also positive in one of five lymphomas and one of three sarcomas.AdditionallyHMB-45 reacted with junctional nevi and componentes of compound neai and not with intradermalnevi and the dermal components of compound nevi.

Effects of sodium valproate combined with levetiracetam in treatment of children epilepsy and its influences on serum S-100βand high mobility group box-1

Objective:To explore the effects of sodium valproate combined with levetiracetam in the treatment of children epilepsy,and its influences on serum S-100βand high mobility group box-1(HMGB-1)in children with epilepsy.Methods:A total of 160 children who were diagnosed as epilepsy in Baogang Hospital of Inner Mongolia from July 2016 to October 2018 were selected as research objects.They were randomly divided into the study group(n=80)and the control group(n=80)by the random number table method,i.e.,they were treated with sodium valproate combined with levetiracetam and sodium valproate alone,respectively.After 16 weeks of treatment,the effective rates of epileptic seizure treatment and the improvement of epileptiform discharge were evaluated,and chi-square test was used for statistical comparison.The related indicators,including serum tumor necrosis factor-(TNF-α),hypersensitive C-reactive protein(hs-CRP),homocysteine(Hcy),haematocrit(HCT),erythrocyte sedimentation rate(ESR),serum S-100βand HMGB-1,were measured before and after treatment.Paired t-test was used for the comparison in the above indicators within a group before and after treatment;group t-test was used for the comparison between two groups.Chi-square test was used for the comparison in the rate of adverse reactions during treatment between two groups.The study was approved by Ethics Committee of Baogang Hospital(Approval No.:BG201606073),and all children’s guardians were required to sign informed consent forms for clinical study.There were no statistically significant differences between two groups in general clinical data(p>0.05),such as sex constituent ratio,age,the course of disease,the frequency of epileptic seizure per year before treatment,the incidence of epileptiform discharge before treatment and the constituent ratio of types of epileptic seizure,etc.Results:1)After treatment,the effective rates of epileptic seizure treatment and the improvement of epileptiform discharge in the study group were 92.5%(74/80)and 85.0%(68/80)respectively,which were both significantly higher than those in the control group[68.8%(55/80)and 58.8%(47/80)],and the differences were statistically significant(Х^(2)=14.444,13.635;p<0.001).2)In the study group,the levels of serum TNF-α,hs-CRP and Hcy,as well as HCT and ESR after treatment were(53.1±14.0)pg/ml,(5.0±2.5)mg/L,(12.5±3.1)μmol/L,(38.1±5.1)%and(3.0±0.5)mm/h respectively,which were all significantly lower than those[(107.9±17.8)pg/ml,(10.1±2.5)mg/L,(42.2±5.8)μmol/L,(45.3±4.5)%and(5.2±0.6)mm/h]before treatment,and all the differences were statistically significant(t=21.644,12.902,40.393,9.468,25.194;p<0.001).In the control group,the levels of serum TNF-α,hs-CRP and Hcy,as well as HCT and ESR after treatment were(60.6±17.8)pg/ml,(8.2±2.2)mg/L,(15.2±3.1)μmol/L,(40.2±3.4)%and(4.5±0.6)mm/h respectively,which were all significantly lower than those[(112.4±14.3)pg/ml,(9.3±3.8)mg/L,(41.1±2.8)μmol/L,(44.6±5.5)%and(5.4±0.8)mm/h]before treatment,and all the differences were statistically significant(t=20.292,2.241,55.456,3.320,8.050;p<0.05).After treatment,the above indicators in the study group were all significantly lower than those in the control group,and all the differences were statistically significant(t=2.962,8.595,5.508,3.064,17.178;p<0.05).3)In the study group,the levels of serum S-100βand HMGB-1 after treatment were(0.65±0.38)μg/L and(5.3±2.4)μg/L respectively,which were significantly lower than those[(0.91±0.32)μg/L and(8.1±2.0)μg/L]before treatment,and the differences were statistically significant(t=4.681,8.020;p<0.001).In the control group,the levels of serum S-100βand HMGB-1 after treatment were(0.78±0.27)μg/L and(6.4±2.2)μg/L respectively,which were significantly lower than those[(0.88±0.25)μg/L and(7.9±1.7)μg/L]before treatment,and the differences were statistically significant(t=2.431,p=.016;t=4.826,p<0.001).After treatment,the levels of serum S-100βand HMGB-1 in the study group were significantly lower than those in the control group,and the differences were statistically significant(t=2.495,p=.014;t=2.840,p=.005).4)There was no significant difference between two groups in the rate of adverse reactions,such as nausea,vomiting,poor appetite,dizziness,drowsiness,hepatic and renal injury during treatment(p>0.05).Conclusions:The efficacy of sodium valproate combined with levetiracetam is obviously better than that of sodium valproate alone in the treatment of children epilepsy.The children patients’serum S-100βand HMGB-1 are more significantly reduced,resulting in a lower rate of adverse reactions,which has a certain clinical value.

The Expression and Distribution of S-100 Protein and CD83 in Thyroid Tissues of Autoimmune Thyroid Diseases

To investigate the expression and distribution of S-100 protein and CD83 in the thyroid tissues of autoimmune thyroid diseases(ATDs),and to study the role of the dendritic cells in the pathogenesis of ATDs, immunohistochemical staining was used on pathological tissues of 20 patients with Hashimoto's thyroiditis(HT) and 20 patients with Graves' disease(GD) to check the expression and distribution of S-100 protein and CD83. Compared with control group(20 cases of thyroid follicular adenoma,TFA),the higher expressions of S-100 in HT(139.38±5.92 vs 59.47±11.69) and GD(119.42±14.48 vs 59.47±11.69) were observed respectively(p< 0.001).The increased positive expressions of CD83 which is known as a marker of mature and activated DCs in HT(22.58±13.96 vs 5.19±8.08) and GD(29.92±14.43 vs 5.19±8.08) were also found respectively(p<0.001). Serum TPO antibody(TPO-Ab,67.3±11.6%) and Tg antibody(Tg-Ab,59.8±10.1%) in HT were higher than those in GD(28.4±5.7%,23.1±4.9%) and TFA(6.1±3.4%,7.2±4.6%)(p<0.01).Serum TR-Ab in GD(16.3 ±5.6 U/L) was higher than those in HT(4.8±2.3 U/L) and TFA(2.5±1.2 U/L)(p<0.01).Our findings suggest that the high expression of DCs' markers may be related to the pathogenesis of HT and GD.The upregulation of both the number and the matured functions of DCs,may lead to present more antigens and to produce more auto-antibodies(such as Tg-Ab and TPO-Ab in HT,TR-Ab in GD),which may be involved in pathogenesis of the autoimmune thyroid diseases.Cellular & Molecular Immunology.2004;1(5):378-382.

高原地区肺性脑病患者血清NSE和S-100β蛋白水平变化及与病情的关系

目的观察高原地区肺性脑病患者血清神经元特异性烯醇化酶(NSE)和S-100β蛋白(S-100β)水平的变化,探讨血清NSE和S-100β水平与病情的相关性。方法 对64例高原地区肺性脑病患者(A组)、228例肺心病患者(B组)采用以法检测血清NSE、S-100β含量,用血气分析仪测定动脉血氧分压(PaO2)和二氧化碳分压(PaCO2),并选择同海拔同年龄的50例健康人作对照(C组)。结果 A组和B组的PaCO2、血清NSE和S-100β水平显著高于C组,PaO2水平显著低于C组(均P<0.01);A组与B组之间比较,各项指标亦有显著性差异(均P<0.01)。在A组中,重型和中型患者的PaCO2、血清NSE和S-100β水平显著高于轻型患者,PaO2水平显著低于轻型患者(均P<0.01);重型与中型之间比较,各项指标亦有显著性差异(均P<0.01)。A组PaO2与血清NSE和S-100β呈显著负相关(r=-0.823、-0.792,均P<0.01),PaCO2与血清NSE和S-100β呈显著正相关(r=0.786、0.802,均P<0.01)。A组中,55例患者神经精神症状消失且病情稳定1周后,血清NSE、S-100β和PaCO2较治疗前显著降低,PaO2显著升高(均P<0.01),9例患者死亡前,血清NSE和S-100β较治疗前进一步升高(均P<0.01)。结论 血清NSE和S-100β的含量与肺性脑病患者病情严重程度密切相关,可作为判断其病情程度、估计预后的重要指标。

脑特异性蛋白质及其对神经系统疾病的临床意义

中枢神经系统（central nervoussystem，CNS）存在一些特殊的蛋白质，主要分布于脑组织中，某些在周围神经系统（peripheral nervous system，PNS）有少量分布，在其他组织器官中含量很低，称为脑特异性蛋白质（brain—specific protein），主要包括神经元特异性烯醇化酶（neuronspecific enolase，NSE）、髓鞘碱性蛋白（myelin basic protein，MBP）、S-100b蛋白（S．100protein，S-100）和肌酸激酶脑型同工酶（CK—BB）。现将上述几种蛋白质综述如下。

Schwannoma of the rectum:A case report and literature review

Schwannoma is a tumor originating from the Schwann cells. Gastrointestinal schwannomas are uncommon stromal tumors of the intestinal tract and, in particular, rectal schwannomas are extremely rare. In fact, it is well established that schwannomas appear more frequently in the stomach and in the small intestine, while location in the colon or in the rectum is uncommon. Reading the literature, only few cases of rectal schwannoma have been reported. Their diagnosis is confirmed by the immunohisto chemical panel(S-100 protein). When these tumors are located in the colon and in the rectum, radical excision with wide margins is mandatory, due to their tendency to recur locally or become malignant, if left untreated. In the present study, we describe a case of a rectal schwannoma occured in a 72-year-old man, presented as a small polypoid lesion, which was successfully removed in toto by hot-biopsy, during the same endoscopy, due to the dimensons. No recurrence of the lesion was observed after 6 mo of follow-up.

rhBNP治疗脓毒症相关性脑病患者对S-100β、NSE水平的影响

目的:研究重组人脑利钠肽(rhBNP)治疗脓毒症相关性脑病患者对S-100β蛋白(S-100β)、神经元特异性烯醇化酶(NSE)水平的影响。方法:选取我院接受治疗的90例脓毒症相关性脑病患者,按照随机数字表法将其随机分为观察组和对照组,每组45例,对照组采用常规方式进行治疗,观察组在对照组基础上注射rhBNP,比较两组临床疗效,治疗前后血流动力学[平均动脉压(MAP)、心脏指数(CI)、中心静脉压(CVP)]、神经细胞因子[S-100β、NSE、N端脑钠肽前体(NT-proBNP)],以及治疗期间不良反应发生情况。结果:观察组治疗总有效率为84.44%,显著高于对照组64.44%。观察组治疗后MAP、CVP水平显著低于治疗前及对照组,CI水平显著高于治疗前及对照组。观察组治疗后S-100β、NSE、NT-proBNP水平显著低于治疗前及对照组。两组治疗期间不良反应总发生率分别为24.44%、15.56%。结论:rhBNP能有效改善脓毒症相关性脑病患者的S-100β、NSE水平,并且具有良好的临床安全性。

Unusual histological variant of malignant peripheral nerve sheath tumor with rhabdomyoblastic differentiation

Malignant peripheral nerve sheath tumor(MPNST)with rhabdomyoblastic differentiation is called as malignant triton tumor(MTT). It is highly aggressive soft tissue tumor with higher local recurrence rate. MTT has poor prognosis than MPNST. MTT seems to be more aggressive in patients with neurofibromatosis(NF-1). We herein, reporting an interesting case of 55 years male with multiple neurofibromas all over the body since 30 years and multiple café-au-lait spots, diagnosed as NF-1. Since 6 years, he had an enlarged mass in left thigh. Wide excision of mass was done. On histopathological examination revealed the diagnosis of MTT and diagnosis of which was confirmed on immunohistochemistry.

病毒性脑膜炎患儿血清中枢神经特异性蛋白β髓鞘碱性蛋白的表达与临床预后的关系

目的 分析病毒性脑膜炎(VE)患儿血清中枢神经特异性蛋白β(S-100β)、髓鞘碱性蛋白(MBP)的表达及与临床预后不良的关系。方法 选取信阳市第四人民医院2019年1月至2021年1月收治的110例VE患儿作为研究对象,全部患儿均进行综合治疗,于治疗7 d评估患儿治疗效果,并分为预后良好组和预后不良组。于入院时检测血清S-100β、MBP、中性粒细胞CD64表达,分析入院时VE患儿血清S-100β、MBP的表达与临床预后不良的关系。结果 110例VE患儿临床预后不良26例,占23.6%;临床预后良好84例,占76.4%;预后不良组中性粒细胞CD64、血清S-100β、MBP表达高于预后良好组,差异有统计学意义(P<0.05);经Logistic回归分析结果显示,中性粒细胞CD64、血清S-100β、MBP高表达可能是VE患儿临床预后不良的风险因子(OR>1,P<0.05);绘制受试者工作特征(ROC)曲线发现,血清S-100β、MBP单一及联合预测VE患儿临床预后不良风险的曲线下面积(AUC)分别为0.811、0.791、0.835,AUC均>0.70,有一定预测价值,且当二者截断值分别取1.705μg/L、15.305μg/L时,预测价值最佳。结论 VE患儿血清S-100β、MBP呈高表达,且血清S-100β、MBP与VE患儿临床预后不良有关。

Effect of human urinary kallikein on the neurological function and intracranial hemodynamics in patients with acute cerebral infarction

Objective:To explore the effect of human urinary kallikein (HUK) on the neurological function and intracranial hemodynamics in patients with acute cerebral infarction.Methods:A total of 150 patients with acute cerebral infarction who were admitted in our hospital were included in the study and divided into the observation group and the control group with 75 cases in each group according to different treatment protocols. The patients in the two groups were given routine drugs for conservative treatment. On this basis, the patients in the observation group were given HUK. NSE and S-100 protein before treatment, 3, 7, 14, and 28 d after treatment in the two groups were compared. TCD was used to detect the cerebral hemodynamics. NIHSS and BI were used to evaluate the improved degree of neurological function and daily living activities before treatment, 1 and 3 months after treatment.Results: NSE and S-100 protein 3, 7, 14, and 28 d after treatment in the observation group were significantly lower than those in the control group (P<0.05). The blood flow rate of left vertebral artery, right vertebral artery, and basilar artery after treatment in the two groups was significantly elevated (P<0.05). The blood flow rate of left vertebral artery, right vertebral artery, and basilar artery after treatment in the observation group was significantly higher than that in the control group (P<0.05). NIHSS and BI scores 1 and 3 months after treatment in the observation group were significantly superior to those in the control group (P<0.05).Conclusions: HUK can effective reduced the brain tissue injury in patients with acute cerebral infarction, and promote the recovery of neurological function, with an accurate efficacy.

Histopathological Characteristics of Folliculo-stellate Cells in Pituitary Glands of Wild Type,Obese and High-fat Diet Induced Mice

The anterior pituitary gland regulates growth,metabolism,and reproduction by secreting hormones.Folliculo-stellate cells(FSC)are non-endocrine cells located among hormone-producing cells in the anterior pituitary glands,but little is known about the exact roles of those cells.Although,with their net organization,they seem to have an important role in the hormonal cells regulation and maintenance.In this work,the first ever made in this area,33 pituitaries of three groups of mice(18 wild type[WT],11 genetically obese[OB]and 4 under a high-fat diet[HFD])were studied in order to determine if there was any relation between the number of FSC and alterations of the basal metabolism in each group of mice.For that,immunohistochemical staining using the S-100 protein was used and also the Image-J software,to calculate the percentage of FSC present in each sample.The authors found that,although there wasn’t any significant difference between WT and OB mice,the group of HFD mice tend to have substantially higher percentage of FSC than the mice from other groups.This might suggest some yet unknown link between diet,precisely with a high-fat diet,and the presentation of FSC in the anterior pituitary.