S-100抗体、人黑色素瘤抗体（melarlon]al black-45，MB45）和黑色素瘤抗原T细胞（melanoma antigenreeognized by Tcell-1，MART-1）是签别良性或恶性皮肤色素痣和黑色素瘤的主要标记物，但对结膜黑色素细胞增生物的诊断仍存在争议^[1-...S-100抗体、人黑色素瘤抗体（melarlon]al black-45，MB45）和黑色素瘤抗原T细胞（melanoma antigenreeognized by Tcell-1，MART-1）是签别良性或恶性皮肤色素痣和黑色素瘤的主要标记物，但对结膜黑色素细胞增生物的诊断仍存在争议^[1-2].本研究拟筛选可鉴别结膜良性或恶性黑色素病变的免疫标志物，展开更多
The histologic diagnosis of desmoplastic melanoma can be challenging in circumstances in which biopsy specimens are small, or there are unsuspected clinical settings, re-excision scars, and unusual sites. This is part...The histologic diagnosis of desmoplastic melanoma can be challenging in circumstances in which biopsy specimens are small, or there are unsuspected clinical settings, re-excision scars, and unusual sites. This is particularly true when the overlying junctional component is absent or the spindle cells lack melanin pigment. In these instances, the importance of immunohistochemistry cannot be overemphasized. S-100 protein is the primary immunohistochemical stain used for this purpose, with a sensitivity approaching 90%. HMB-45, although a more specific marker for melanocytes, is less sensitive and often negative in these cases. In addition, NGFR, a marker of Schwannian differentiation, has been shown to be a useful confirmatory stain for desmoplastic melanoma, with staining intensity comparable with, or better than that of S-100 protein. We report 2 cases of desmoplastic melanomas that stained only focally and weakly with S-100 protein, but showed diffuse and intense staining with NGFR. In both cases, S-100 staining could have been interpreted as non-confirmatory, thus misguiding the diagnosis. We suggest that NGFR can be a useful complementary marker to S-100 in those desmoplasticmelanomas in which staining for S100 protein is only focal or weak.展开更多
文摘S-100抗体、人黑色素瘤抗体（melarlon]al black-45，MB45）和黑色素瘤抗原T细胞（melanoma antigenreeognized by Tcell-1，MART-1）是签别良性或恶性皮肤色素痣和黑色素瘤的主要标记物，但对结膜黑色素细胞增生物的诊断仍存在争议^[1-2].本研究拟筛选可鉴别结膜良性或恶性黑色素病变的免疫标志物，
文摘The histologic diagnosis of desmoplastic melanoma can be challenging in circumstances in which biopsy specimens are small, or there are unsuspected clinical settings, re-excision scars, and unusual sites. This is particularly true when the overlying junctional component is absent or the spindle cells lack melanin pigment. In these instances, the importance of immunohistochemistry cannot be overemphasized. S-100 protein is the primary immunohistochemical stain used for this purpose, with a sensitivity approaching 90%. HMB-45, although a more specific marker for melanocytes, is less sensitive and often negative in these cases. In addition, NGFR, a marker of Schwannian differentiation, has been shown to be a useful confirmatory stain for desmoplastic melanoma, with staining intensity comparable with, or better than that of S-100 protein. We report 2 cases of desmoplastic melanomas that stained only focally and weakly with S-100 protein, but showed diffuse and intense staining with NGFR. In both cases, S-100 staining could have been interpreted as non-confirmatory, thus misguiding the diagnosis. We suggest that NGFR can be a useful complementary marker to S-100 in those desmoplasticmelanomas in which staining for S100 protein is only focal or weak.
