The significant inhibition action of Gypenosides (GP) to many kinds or tumor strain wesreported before. The erfects of GP on the ultrastructures, without any findiys in present litrature,of S-180 sarcoma in mouse were...The significant inhibition action of Gypenosides (GP) to many kinds or tumor strain wesreported before. The erfects of GP on the ultrastructures, without any findiys in present litrature,of S-180 sarcoma in mouse were studied through electron microscope in this article. The results indicated that the ultrastructures of.S-180 sarcoma cells were destructed obviously by the administratiouof GP to the tumor-bearing mouse with a Pattern or dose-depeudant, especially to the cellular nuclear. We saw both apoptosis and necrosis in morphologic alterations in tumor cells, suck as a reductionin cellular volume. an increase in cytoplasm, uucleoplasm electron density and condensation of nuclear chromatiu either to periphery or the nuclear membrane or inclumps within the cell, lots of inthe cytoplasm and apoptotic body in some turner cells or some cytoplast breaking into small frag- ments etc.展开更多
The control and counter-control processes between the host and cancer cells are all interrelated to the bioelectromagnetic phenomena. Thus many people, both at home and abroad, have done experiments by using magnetic ...The control and counter-control processes between the host and cancer cells are all interrelated to the bioelectromagnetic phenomena. Thus many people, both at home and abroad, have done experiments by using magnetic field to restrain and kill cancer cells. However, because the biological effect and the mechanism of the magnetic field are very complicated, further work is still needed.展开更多
This study examined the effect of saponins from Tupistra chinensis Bak （STCB） on the growth of sarcoma S-180 cells in vitro and in mouse xenografts as well as the underlying mechanisms. Cell proliferation was assess...This study examined the effect of saponins from Tupistra chinensis Bak （STCB） on the growth of sarcoma S-180 cells in vitro and in mouse xenografts as well as the underlying mechanisms. Cell proliferation was assessed by MTT assay. Cell cycle distribution was determined by flow cytometry. Sarcoma S-180 tumor-bearing mice were treated with different doses of STCB with 10 μg/mL 5-fluorouracil （5-Fu） as a positive control. The activity of nuclear factor （NF）-κB was detected by gel mobility shift assay. The mRNA level of NF-κB was determined by real-time quantitative RT-PCR. The results showed that in vitro STCB inhibited the growth of S-180 cells in a concentration-dependent manner, which was accompanied by cell cycle arrest at S-phase. In vivo STCB significantly inhibited the growth of S-180 tumor mouse xenografts in a dose-dependent manner with apparent induction of cell apoptosis. Moreover, STCB inhibited the activity of NF-rd3 p65 and reduced the expression of NF-κB p65 mRNA in mouse xenografts. It was concluded that STCB inhibits the proliferation and cell cycle progression of S- 180 cells by suppressing NF-κB signaling in mouse xenografts. Our findings suggest STCB is a promising agent for the treatment of sarcoma.展开更多
文摘The significant inhibition action of Gypenosides (GP) to many kinds or tumor strain wesreported before. The erfects of GP on the ultrastructures, without any findiys in present litrature,of S-180 sarcoma in mouse were studied through electron microscope in this article. The results indicated that the ultrastructures of.S-180 sarcoma cells were destructed obviously by the administratiouof GP to the tumor-bearing mouse with a Pattern or dose-depeudant, especially to the cellular nuclear. We saw both apoptosis and necrosis in morphologic alterations in tumor cells, suck as a reductionin cellular volume. an increase in cytoplasm, uucleoplasm electron density and condensation of nuclear chromatiu either to periphery or the nuclear membrane or inclumps within the cell, lots of inthe cytoplasm and apoptotic body in some turner cells or some cytoplast breaking into small frag- ments etc.
文摘The control and counter-control processes between the host and cancer cells are all interrelated to the bioelectromagnetic phenomena. Thus many people, both at home and abroad, have done experiments by using magnetic field to restrain and kill cancer cells. However, because the biological effect and the mechanism of the magnetic field are very complicated, further work is still needed.
文摘This study examined the effect of saponins from Tupistra chinensis Bak （STCB） on the growth of sarcoma S-180 cells in vitro and in mouse xenografts as well as the underlying mechanisms. Cell proliferation was assessed by MTT assay. Cell cycle distribution was determined by flow cytometry. Sarcoma S-180 tumor-bearing mice were treated with different doses of STCB with 10 μg/mL 5-fluorouracil （5-Fu） as a positive control. The activity of nuclear factor （NF）-κB was detected by gel mobility shift assay. The mRNA level of NF-κB was determined by real-time quantitative RT-PCR. The results showed that in vitro STCB inhibited the growth of S-180 cells in a concentration-dependent manner, which was accompanied by cell cycle arrest at S-phase. In vivo STCB significantly inhibited the growth of S-180 tumor mouse xenografts in a dose-dependent manner with apparent induction of cell apoptosis. Moreover, STCB inhibited the activity of NF-rd3 p65 and reduced the expression of NF-κB p65 mRNA in mouse xenografts. It was concluded that STCB inhibits the proliferation and cell cycle progression of S- 180 cells by suppressing NF-κB signaling in mouse xenografts. Our findings suggest STCB is a promising agent for the treatment of sarcoma.
