Apoptosis of tumor cells have become a new standard for chemotherapy. It is useful to demonstrate induction of apoptosis in tumor cells by anti-cancer drugs in vivo. We reported the results of apoptosis induction in m...Apoptosis of tumor cells have become a new standard for chemotherapy. It is useful to demonstrate induction of apoptosis in tumor cells by anti-cancer drugs in vivo. We reported the results of apoptosis induction in murine tumor cell line S-180 and it's resistant cell line S-180R by adriamycin in different dose and different time. We found that apoptosis in S-180 cells could be induced by low dose of adriamycin, the apoptosis was started at 24 h. after the administration, and reached to 62.5% of the cells to apptosis until 72 h. Comparison with the parental cell line, only 13% of S-180R cells were apoptosed. At high dose, 20% of S-180R cells were apoptosed, whereas, almost all S-180 cells were killed in the same time. The lymphocytes were appeared in abdominal cavity of the mice after treatment of adriamycin for 24 h. It was very interested to find out that there was no lymphocyte left in the abdominal cavity of the mice with S-180R cells treated at high dose of adriamycin.展开更多
Drug resistance is a very important problem for cancer chemotherapy. The ma-jor type of drug resistance is due to the overexpression of P-glycoproteins (P-170) in cancer cells. P-170 could pump the drug out of the cel...Drug resistance is a very important problem for cancer chemotherapy. The ma-jor type of drug resistance is due to the overexpression of P-glycoproteins (P-170) in cancer cells. P-170 could pump the drug out of the cells, so the drug could not be accumulated enough to kill the cell-s. We had developed the adriamycin resistant cell line S-180R in BABL/c mice. The cells overexpressP-170 stably. Different dosages of moxa cone were used on Guanyuan (CV 4) point of the mice.Stimulation of drug accumulation was found after administration of moxibustion for 30 min and a doseresponse manner was shown below 400 mg of moxa cone, but a little decrease of the stimulation wasobtained when using 600 mg of moxa cone. These results confirmed positive effects of moxibustion onovercoming the drug resistance through stimulation of drug accumulation in the cancer cells.展开更多
Objective: In order to assess the genetic stability of doxorubicin resistance sarcoma S 180R cell line in vivo . Methods: The drug resistant genes and molecules were examined by flow cytometry, Southern blot, Nort...Objective: In order to assess the genetic stability of doxorubicin resistance sarcoma S 180R cell line in vivo . Methods: The drug resistant genes and molecules were examined by flow cytometry, Southern blot, Northern blot and RT PCR. Results: The results showed that drug efflux in S 180R increased nearly 100 folds, as compared with its parent cells, the rate of half peak width resistant cell/peak high decreased from 0.56 to 0.23 measured by flow cytometry after two years. The mdr1 gene amplified and overexpressed significantly in S 180R and the expression of topoisomerase II α gene decreased remarkably in S 180R. There was no significant different of the MRP expression between S 180R and S 180. Conclusion: These results indicated that drug resistance of S 180R was maintained and also increased. The major mechanism of drug resistance is the amplification and overexpression of mdr1 gene, the decreased expression of topoisomerase II α also contributed to it. So, S 180R is an ideal experimental model for the study of doxorubicin resistance and its reversion in vivo .展开更多
文摘Apoptosis of tumor cells have become a new standard for chemotherapy. It is useful to demonstrate induction of apoptosis in tumor cells by anti-cancer drugs in vivo. We reported the results of apoptosis induction in murine tumor cell line S-180 and it's resistant cell line S-180R by adriamycin in different dose and different time. We found that apoptosis in S-180 cells could be induced by low dose of adriamycin, the apoptosis was started at 24 h. after the administration, and reached to 62.5% of the cells to apptosis until 72 h. Comparison with the parental cell line, only 13% of S-180R cells were apoptosed. At high dose, 20% of S-180R cells were apoptosed, whereas, almost all S-180 cells were killed in the same time. The lymphocytes were appeared in abdominal cavity of the mice after treatment of adriamycin for 24 h. It was very interested to find out that there was no lymphocyte left in the abdominal cavity of the mice with S-180R cells treated at high dose of adriamycin.
文摘Drug resistance is a very important problem for cancer chemotherapy. The ma-jor type of drug resistance is due to the overexpression of P-glycoproteins (P-170) in cancer cells. P-170 could pump the drug out of the cells, so the drug could not be accumulated enough to kill the cell-s. We had developed the adriamycin resistant cell line S-180R in BABL/c mice. The cells overexpressP-170 stably. Different dosages of moxa cone were used on Guanyuan (CV 4) point of the mice.Stimulation of drug accumulation was found after administration of moxibustion for 30 min and a doseresponse manner was shown below 400 mg of moxa cone, but a little decrease of the stimulation wasobtained when using 600 mg of moxa cone. These results confirmed positive effects of moxibustion onovercoming the drug resistance through stimulation of drug accumulation in the cancer cells.
文摘Objective: In order to assess the genetic stability of doxorubicin resistance sarcoma S 180R cell line in vivo . Methods: The drug resistant genes and molecules were examined by flow cytometry, Southern blot, Northern blot and RT PCR. Results: The results showed that drug efflux in S 180R increased nearly 100 folds, as compared with its parent cells, the rate of half peak width resistant cell/peak high decreased from 0.56 to 0.23 measured by flow cytometry after two years. The mdr1 gene amplified and overexpressed significantly in S 180R and the expression of topoisomerase II α gene decreased remarkably in S 180R. There was no significant different of the MRP expression between S 180R and S 180. Conclusion: These results indicated that drug resistance of S 180R was maintained and also increased. The major mechanism of drug resistance is the amplification and overexpression of mdr1 gene, the decreased expression of topoisomerase II α also contributed to it. So, S 180R is an ideal experimental model for the study of doxorubicin resistance and its reversion in vivo .
