In this paper,we shall exploit the Freud method in the Classical operator approximation theory to im- prove known quantitative estimalions with an emphasis on Calculah' on the generic constants.
We consider the harmonic analysis associated with the Dunkl operators on Rd. We study the Dunkl mean-periodic functions on the space ε(Rd) (the space of C∞-functions). We characterize also the continuous linear mapp...We consider the harmonic analysis associated with the Dunkl operators on Rd. We study the Dunkl mean-periodic functions on the space ε(Rd) (the space of C∞-functions). We characterize also the continuous linear mappings from ε(Rd) into itself which commute with the Dunkl operators.展开更多
In the present paper, a new difference matrix via difference operator D is introduced. Let x = （xk） be a sequence of real numbers, then the difference operatorD is defined by D（x）n =∑kn=0（-1）k（n-kn）xk,where ...In the present paper, a new difference matrix via difference operator D is introduced. Let x = （xk） be a sequence of real numbers, then the difference operatorD is defined by D（x）n =∑kn=0（-1）k（n-kn）xk,where n = 0,1,2,3,.... Several interestingproperties of the new operator D are discussed.展开更多
A digital arc welding power supply was designed with the advanced reduced instruction set computer machine （ARM） and embedded real-time multi-task operating system micro C/OS- Ⅱ. The ARM, with its powerful calculat...A digital arc welding power supply was designed with the advanced reduced instruction set computer machine （ARM） and embedded real-time multi-task operating system micro C/OS- Ⅱ. The ARM, with its powerful calculating speed and complete peripheral equipments, is very suitable to work as the controller of the digital power supply. The micro C/OS- Ⅱ transplanted in ARM, helps to improve the respondent speed against various welding signals, as well as the reliability of the controlling software. The welding process consists of nine tasks. The tasks of great significance on reliability of the welder, for example, the A/D conversion of current and voltage, enjoy top priority. To avoid simultaneous-sharing on A/D converter and LCD module, two semaphores are introduced in to ensure the smooth performance of the welding power supply. Proven by experiments ,the ARM and the micro C/OS- Ⅱ can greatly improve both the respondent speed and the reliability of the digital welder.展开更多
Store-operated Ca2＋ channels （SOCs） are plasma membrane Ca2＋ permeable channels activated by depletion of intracellular Ca2＋ store. Ca2＋ entry through SOCs is known as store-operated Ca2＋ entry （SOCE）, which ...Store-operated Ca2＋ channels （SOCs） are plasma membrane Ca2＋ permeable channels activated by depletion of intracellular Ca2＋ store. Ca2＋ entry through SOCs is known as store-operated Ca2＋ entry （SOCE）, which plays an important role in the functional regulation of airway smooth muscle cells （ASMCs）. Protein kinase C （PKC） has been shown to have an activating or inhibiting effect on SOCE, depending on cell types and PKC isoforms that are involved. In ASMCs, the effect of PKC on SOCE has not been elucidated so far. In this study, the role of PKC in the activation of SOCE in rat ASMCs was examined by using Ca2＋ fluorescence imaging technique. The results showed that acute application of PKC activators PMA and PDBu did not affect SOCE induced by the sarcoplasmic reticulum Ca2＋-ATPase （SERCA） inhibitor thapsigargin. The non-selective PKC inhibitor chelerythrine significantly inhibited thapsigargin- and bradykinin-induced SOCE. RT-PCR assay identified PKCα, δ and ε isoforms in rat ASMCs. PKCα-selective inhibitor G6976 and PKCε-inhibiting peptide Epsilon-V1-2 had no effect on SOCE; by contrast, PKCδ-selective inhibitor rottlerin attenuated SOCE dramatically, suggesting that PKCδ was the major PKC isoform involved in the activation of SOCE in ASMCs. Moreover, PKC down-regulation by extended exposure to high doses of PMA or PDBu also reduced SOCE, confirming the essential role of PKC in the activation of SOCE in ASMCs. In addition, PKC down-regulation did not influence the expression of stromal interaction molecule 1 （STIM1） and Orai1, two elementary molecules in the regulation and activation of SOCs. These results identified PKCδ as an essential PKC isoform involved in the activation of SOCE, and confirmed that PKC regulates the function of ASMCs in a SOCE-dependent manner.展开更多
Hepatitis C virus(HCV)helicase is a molecular motor that splits nucleic acid duplex structures during viral replication,therefore representing a promising target for antiviral treatment.Hence,a detailed understanding ...Hepatitis C virus(HCV)helicase is a molecular motor that splits nucleic acid duplex structures during viral replication,therefore representing a promising target for antiviral treatment.Hence,a detailed understanding of the mechanism by which it operates would facilitate the development of efficient drug-assisted therapies aiming to inhibit helicase activity.Despite extensive investigations performed in the past,a thorough understanding of the activity of this important protein was lacking since the underlying internal conformational motions could not be resolved.Here we review investigations that have been previously performed by us for HCV helicase.Using methods of structure-based computational modelling it became possible to follow entire operation cycles of this motor protein in structurally resolved simulations and uncover the mechanism by which it moves along the nucleic acid and accomplishes strand separation.We also discuss observations from that study in the light of recent experimental studies that confirm our findings.展开更多
An experimental advanced superconducting tokamak （EAST） operation window with the lower hybrid current drive （LHCD） in H-mode is estimated by using a eore-SOL-divertor （C-S-D） model validated by the present EAST...An experimental advanced superconducting tokamak （EAST） operation window with the lower hybrid current drive （LHCD） in H-mode is estimated by using a eore-SOL-divertor （C-S-D） model validated by the present EAST divertor experiments. The operation window consists of four limits including two usual limits, one of which is the maximum allowable heat load onto the divertor plate, and two additional limits associated with the LHCD. The predictive EAST operation window is not qualified to fulfill its mission for high input power. To extend the operation window, gas puffing and impurity seeding are presented as two effective methods. In addition, the effect of the LHCD current on the operation window is also discussed. Our numerical analysis results provide a reference for the safe operation of EAST experiments with LHCD in future.展开更多
文摘In this paper,we shall exploit the Freud method in the Classical operator approximation theory to im- prove known quantitative estimalions with an emphasis on Calculah' on the generic constants.
文摘We consider the harmonic analysis associated with the Dunkl operators on Rd. We study the Dunkl mean-periodic functions on the space ε(Rd) (the space of C∞-functions). We characterize also the continuous linear mappings from ε(Rd) into itself which commute with the Dunkl operators.
文摘In the present paper, a new difference matrix via difference operator D is introduced. Let x = （xk） be a sequence of real numbers, then the difference operatorD is defined by D（x）n =∑kn=0（-1）k（n-kn）xk,where n = 0,1,2,3,.... Several interestingproperties of the new operator D are discussed.
基金Supported by the National Natural Science Foundation of China under Grant No 50575074by the Scientific and TechnologicalProject of Guangdong Province, China, under Grant No 2003A1040310
文摘A digital arc welding power supply was designed with the advanced reduced instruction set computer machine （ARM） and embedded real-time multi-task operating system micro C/OS- Ⅱ. The ARM, with its powerful calculating speed and complete peripheral equipments, is very suitable to work as the controller of the digital power supply. The micro C/OS- Ⅱ transplanted in ARM, helps to improve the respondent speed against various welding signals, as well as the reliability of the controlling software. The welding process consists of nine tasks. The tasks of great significance on reliability of the welder, for example, the A/D conversion of current and voltage, enjoy top priority. To avoid simultaneous-sharing on A/D converter and LCD module, two semaphores are introduced in to ensure the smooth performance of the welding power supply. Proven by experiments ,the ARM and the micro C/OS- Ⅱ can greatly improve both the respondent speed and the reliability of the digital welder.
基金supported by grants from the National Natural Science Foundation of China(No.30871122,No.81072684)
文摘Store-operated Ca2＋ channels （SOCs） are plasma membrane Ca2＋ permeable channels activated by depletion of intracellular Ca2＋ store. Ca2＋ entry through SOCs is known as store-operated Ca2＋ entry （SOCE）, which plays an important role in the functional regulation of airway smooth muscle cells （ASMCs）. Protein kinase C （PKC） has been shown to have an activating or inhibiting effect on SOCE, depending on cell types and PKC isoforms that are involved. In ASMCs, the effect of PKC on SOCE has not been elucidated so far. In this study, the role of PKC in the activation of SOCE in rat ASMCs was examined by using Ca2＋ fluorescence imaging technique. The results showed that acute application of PKC activators PMA and PDBu did not affect SOCE induced by the sarcoplasmic reticulum Ca2＋-ATPase （SERCA） inhibitor thapsigargin. The non-selective PKC inhibitor chelerythrine significantly inhibited thapsigargin- and bradykinin-induced SOCE. RT-PCR assay identified PKCα, δ and ε isoforms in rat ASMCs. PKCα-selective inhibitor G6976 and PKCε-inhibiting peptide Epsilon-V1-2 had no effect on SOCE; by contrast, PKCδ-selective inhibitor rottlerin attenuated SOCE dramatically, suggesting that PKCδ was the major PKC isoform involved in the activation of SOCE in ASMCs. Moreover, PKC down-regulation by extended exposure to high doses of PMA or PDBu also reduced SOCE, confirming the essential role of PKC in the activation of SOCE in ASMCs. In addition, PKC down-regulation did not influence the expression of stromal interaction molecule 1 （STIM1） and Orai1, two elementary molecules in the regulation and activation of SOCs. These results identified PKCδ as an essential PKC isoform involved in the activation of SOCE, and confirmed that PKC regulates the function of ASMCs in a SOCE-dependent manner.
文摘Hepatitis C virus(HCV)helicase is a molecular motor that splits nucleic acid duplex structures during viral replication,therefore representing a promising target for antiviral treatment.Hence,a detailed understanding of the mechanism by which it operates would facilitate the development of efficient drug-assisted therapies aiming to inhibit helicase activity.Despite extensive investigations performed in the past,a thorough understanding of the activity of this important protein was lacking since the underlying internal conformational motions could not be resolved.Here we review investigations that have been previously performed by us for HCV helicase.Using methods of structure-based computational modelling it became possible to follow entire operation cycles of this motor protein in structurally resolved simulations and uncover the mechanism by which it moves along the nucleic acid and accomplishes strand separation.We also discuss observations from that study in the light of recent experimental studies that confirm our findings.
基金supported by National Natural Science Foundation of China(Nos.11105176 and 11105224)
文摘An experimental advanced superconducting tokamak （EAST） operation window with the lower hybrid current drive （LHCD） in H-mode is estimated by using a eore-SOL-divertor （C-S-D） model validated by the present EAST divertor experiments. The operation window consists of four limits including two usual limits, one of which is the maximum allowable heat load onto the divertor plate, and two additional limits associated with the LHCD. The predictive EAST operation window is not qualified to fulfill its mission for high input power. To extend the operation window, gas puffing and impurity seeding are presented as two effective methods. In addition, the effect of the LHCD current on the operation window is also discussed. Our numerical analysis results provide a reference for the safe operation of EAST experiments with LHCD in future.
