地震现场救援要素众多,部署分散,为了统一管理救援要素,综合展示救援态势,设计一种基于B/S架构的地震应急救援态势标绘系统,采用Google Maps API v3实现前端Web GIS功能,加载谷歌地图,标绘震情灾情和救援要素信息,通过AJAX方式与服务器...地震现场救援要素众多,部署分散,为了统一管理救援要素,综合展示救援态势,设计一种基于B/S架构的地震应急救援态势标绘系统,采用Google Maps API v3实现前端Web GIS功能,加载谷歌地图,标绘震情灾情和救援要素信息,通过AJAX方式与服务器端进行数据交互,保存要素信息或者获取服务器端数据,系统设计聊天室功能,登录用户可以在线实时交流。本系统对使用环境要求低,支持多种移动终端,适合地震现场人员使用,实现了分布式要素标绘功能,是地震现场救援行动的有效辅助决策工具。展开更多
Boletus spp. is a wild edible Gelam mushroom, which is only available at coastal area of Bachok, Kelantan. Locals have used this mushroom as a food source and as a traditional medicine. In this study, antioxidants wer...Boletus spp. is a wild edible Gelam mushroom, which is only available at coastal area of Bachok, Kelantan. Locals have used this mushroom as a food source and as a traditional medicine. In this study, antioxidants were extracted using Mili-Q water and methanol. Extraction of 1.0 g of Boletus spp. mushroom pileus and stipe by water produced 0.22 ± 0.09 g and 0.3 ± 0.07 g of total aqueous soluble extracts, respectively. Methanol extraction of 1.0 g Boletus spp. mushroom pileus and stipe produced 0.31 ± 0.08 mg and 0.37 ± 0.05 mg of total methanol soluble extracts, respectively. DPPH assay has been used to determine the value of antioxidant efficiency of each extracts. DPPH assay revealed that the EC50 value of pileus aqueous soluble extract and both pileus and stipe methanol soluble extracts were 1.2 mg/ml. The EC50 value of stipe aqueous soluble extracts was 1.4 mg/ml. The EC50 value of ascorbic acid, an antioxidant control was 0.5 mg/ml. Liquid chromatography- DPPH (LC-DPPH) assay combined with Quadrupole Time-of-flight Mass Spectrometry (QTOF-MS) analysis of 3:2 ratio methanol and water extract has detected 43 antioxidant compounds that were involved in the reduction of DPPH. To identify these compounds, SIMCA 3.0 software that consists of PCA, OPLS-DA and s-plot analyses had been used. PCA analysis had shown the antioxidant activity from sample on DPPH as 73.6%. This antioxidant activity confirmed by OPLS-DA analysis had shown the variability between sample and control as 99.6% with 99.3% prediction. S-plot selection had shown ten antioxidant compounds significantly involved in the DPPH assay. Four of these compounds were identified by the METLIN and NIST databases. The antioxidant metabolites were 2,4,6-trimethylacetophenone imine, glutamyl tryptophan, azatadine and lithocholic acid glycine conjugate. In conclusion, this study revealed that Boletus spp. mushroom is rich with natural antioxidants, which are potentially useful for multiple nutritional and health applications.展开更多
文摘地震现场救援要素众多,部署分散,为了统一管理救援要素,综合展示救援态势,设计一种基于B/S架构的地震应急救援态势标绘系统,采用Google Maps API v3实现前端Web GIS功能,加载谷歌地图,标绘震情灾情和救援要素信息,通过AJAX方式与服务器端进行数据交互,保存要素信息或者获取服务器端数据,系统设计聊天室功能,登录用户可以在线实时交流。本系统对使用环境要求低,支持多种移动终端,适合地震现场人员使用,实现了分布式要素标绘功能,是地震现场救援行动的有效辅助决策工具。
文摘Boletus spp. is a wild edible Gelam mushroom, which is only available at coastal area of Bachok, Kelantan. Locals have used this mushroom as a food source and as a traditional medicine. In this study, antioxidants were extracted using Mili-Q water and methanol. Extraction of 1.0 g of Boletus spp. mushroom pileus and stipe by water produced 0.22 ± 0.09 g and 0.3 ± 0.07 g of total aqueous soluble extracts, respectively. Methanol extraction of 1.0 g Boletus spp. mushroom pileus and stipe produced 0.31 ± 0.08 mg and 0.37 ± 0.05 mg of total methanol soluble extracts, respectively. DPPH assay has been used to determine the value of antioxidant efficiency of each extracts. DPPH assay revealed that the EC50 value of pileus aqueous soluble extract and both pileus and stipe methanol soluble extracts were 1.2 mg/ml. The EC50 value of stipe aqueous soluble extracts was 1.4 mg/ml. The EC50 value of ascorbic acid, an antioxidant control was 0.5 mg/ml. Liquid chromatography- DPPH (LC-DPPH) assay combined with Quadrupole Time-of-flight Mass Spectrometry (QTOF-MS) analysis of 3:2 ratio methanol and water extract has detected 43 antioxidant compounds that were involved in the reduction of DPPH. To identify these compounds, SIMCA 3.0 software that consists of PCA, OPLS-DA and s-plot analyses had been used. PCA analysis had shown the antioxidant activity from sample on DPPH as 73.6%. This antioxidant activity confirmed by OPLS-DA analysis had shown the variability between sample and control as 99.6% with 99.3% prediction. S-plot selection had shown ten antioxidant compounds significantly involved in the DPPH assay. Four of these compounds were identified by the METLIN and NIST databases. The antioxidant metabolites were 2,4,6-trimethylacetophenone imine, glutamyl tryptophan, azatadine and lithocholic acid glycine conjugate. In conclusion, this study revealed that Boletus spp. mushroom is rich with natural antioxidants, which are potentially useful for multiple nutritional and health applications.
