Genetic diversity of the S-type small subunit ribosomal RNA gene of Plasmodium knowlesi isolates from Sabah,Malaysian Borneo and Peninsular Malaysia

Objective:To determine the genetic diversity of Plasmodium(P.)knowlesi isolates from Sabah,Malaysian Borneo and Peninsular Malaysia,targeting the S-type SSU rRNA gene and including aspects of natural selection and haplotype.Methods:Thirty-nine blood samples infected with P.knowlesi were collected in Sabah,Malaysian Borneo and Peninsular Malaysia.The S-type SSU rRNA gene was amplified using polymerase chain reaction,cloned into a vector,and sequenced.The natural selection and haplotype of the S-type SSU rRNA gene sequences were determined using DnaSP v6 and illustrated using NETWORK v10.This study's 39 S-type SSU rRNA sequences and eight sequences from the Genbank database were subjected to phylogenetic analysis using MEGA 11.Results:Overall,the phylogenetic analysis showed no evidence of a geographical cluster of P.knowlesi isolates from different areas in Malaysia based on the S-type SSU rRNA gene sequences.The S-type SSU rRNA gene sequences were relatively conserved and with a purifying effect.Haplotype sharing of the S-type SSU rRNA gene was observed between the P.knowlesi isolates in Sabah,Malaysian Borneo,but not between Sabah,Malaysian Borneo and Peninsular Malaysia.Conclusions:This study suggests that the S-type SSU rRNA gene of P.knowlesi isolates in Sabah,Malaysian Borneo,and Peninsular Malaysia has fewer polymorphic sites,representing the conservation of the gene.These features make the S-type SSU rRNA gene suitable for comparative studies,such as determining the evolutionary relationships and common ancestry among P.knowlesi species.

英国制造——捷豹XJ8和S-TYPE中国上市

虽然在世界汽车制造中，无论是汽车品牌的数量还是汽车的产销量，英国都难以与美国、日本、德国以及法国等相比。但是有一点不可否认，那就是英国在豪华车领域具有无与伦比的地位，像劳斯莱斯、宾利、罗孚、捷豹、阿斯顿·马丁以及莲花等。

Roll Motion Analysis of Deepwater Pipelay Crane Vessel

For a large floating vessel in waves,radiation damping is not an accurate prediction of the degree of roll unlike other degrees of freedom motion.Therefore,to get the knowledge of roll motion performance of deepwater pipelay crane vessels and to keep the vessel working safety,the paper presents the relationship between a series of dimensionless roll damping coefficients and the roll response amplitude operator(RAO).By using two kinds of empirical data,the roll damping is estimated in the calculation flow.After getting the roll damping coefficient from the model test,a prediction of roll motion in regular waves is evaluated.According to the wave condition in the working region,short term statistics of roll motion are presented under different wave parameters.Moreover,the relationship between the maximal roll response level to peak spectral wave period and the roll damping coefficient is investigated.Results may provide some reference to design and improve this kind of vessel.

Genesis of S-type Granites in the Pengshan Sn-polymetallic Ore Field, Northern Jiangxi Province and its Implications

The Pengshan Sn-polymetallic ore field is located in the southeastern part of the Yangtze block,spanning the southeast edge of the MLYDZ and the northern edge of the mid-segment of the Jiangnan Uplift,and on one side of the MLYDZ.The studies of LA-ICP-MS zircon U-Pb chronology and petrogeochemistry for Early Cretaceous acid granites from the Pengshan ore field were carried out in this paper.We report zircon U-Pb geochronology and whole-rock geochemistry for acid granites in the Pengshan ore field.The zircon U-Pb ages of the muscovite-granite,biotite adamellite and granite-porphyry are 127.6±1.7 Ma,126.9±1.6 Ma and 126.6±2.0 Ma,respectively.The granites in Pengshan are characterized by a high silicon content and are rich in alkali.They belong to high-potassium,calc-alkaline,peraluminous granite.The rocks have a relatively high Rb/Ba ratio,and the data points for muscovite-granite and biotite adamellite all fall within the clay-rich sources region,near the pelite-derived end-member,showing that the Pengshan muscovite-granite and biotite adamellite mainly originated from the partial melting of metapelites with high maturity.The transformation of the compressional and extensional tectonics in this region approximately 128 Ma obviously lags behind that in the mid-segment of the Jiangnan Uplift(135 Ma),but occurred earlier than the MLYDZ(126 Ma).The Pengshan ore field extends from the mid-segment of the Jiangnan Uplift to the MLYDZ.Although the tectonic stress field is constrained by the combination of the two secondary tectonic units,the time of tectonic system transformation is closer to the MLYDZ because the spatial orientation of the area is enclosed in the MLYDZ.Relevant geophysical and drilling data confirm the rationality of Pengshan-Ao'xia as a multi-center vertical zoning ore field,and show the scientificity of the prospecting idea of abutting joint between the north-west of Pengshan area and the south-east of Ao'xia area.

Fiber cladding dual channel surface plasmon resonance sensor based on S-type fiber

Fiber cladding surface plasmon resonance(SPR)sensors have few structures,and a clad SPR sensor based on S-type fiber is proposed in this paper.This new type of fiber cladding SPR sensor was formed by electrofusing an S-shaped structure on the fiber to couple the light in the fiber core to the cladding.In this paper,the effects of fiber parameters on the performance of the sensor were studied by simulation and experiment.Based on the conclusion that the smaller the core diameter is,the closer the working band of the SPR resonance is to long wavelengths,and that the geometric characteristics mean that a multimode fiber can receive the fiber cladding light from a small core diameter few-mode fiber,a dual channel SPR sensor with a double S-type fiber cascade was proposed.In the refractive index detection range of 1.333–1.385refractive index units(RIU),the resonant working band of channel I is 627.66 nm–759.78 nm,with an average sensitivity of 2540.77 nm/RIU,and the resonant working band of channel II is 518.24 nm–658.2 nm,with an average sensitivity of2691.54 nm/RIU.The processing method for the S-type fiber cladding SPR sensor is simple,effectively solving the problem of this type of SPR sensor structure and the difficult realization of a dual channel.The sensor is expected to be used in the fields of medical treatment and biological analysis.

Chronology and Genesis of S-type Granites in Hetai District, Guangdong Province: Constraints from LA-ICP-MS Zircon U-Pb Dating and Tourmaline Boron Isotope In-situ Analyses

1 Introduction Hetai district,which is a mountainous area,situated on Guangning and Zhaoqing city,west Guangdong Province.Hetai district is generally located on southwest of South China Caledonian fold belt,east margin of Yunkai post-Caledonian uplift.Multiple type granites are widely distributed in Hetai district,including Caledonian,Indosinian and Yanshanian granites.Based on different

Activation of Sonic Hedgehog Signaling Pathway in S-type Neuroblastoma Cell Lines

The effects of Sonic hedgehog(Shh) signaling pathway activation on S-type neuroblastoma(NB) cell lines and its role in NB tumorigenesis were investigated.Immunohistochemistry was used to detect the expression of Shh pathway components— Patched1(PTCH1) and Gli1 in 40 human primary NB samples.Western blotting and RT-PCR were used to examine the protein expression and mRNA levels of PTCH1 and Gli1 in three kinds of S-type NB cell lines(SK-N-AS,SK-N-SH and SHEP1),respectively.Exogenous Shh was administrated to activate Shh signaling pathway while cyclopamine was used as a selective antagonist of Shh pathway.S-type NB cell lines were treated with different concentrations of Shh or/and cyclopamine for different durations.Cell viability was measured by using MTT method.Apoptosis rate and cell cycle were assayed by flow cytometry.The xenograft experiments were used to evaluate the role of Shh pathway in tumor growth in immunodeficient mice.High-level expression of PTCH1 and Gli1 was detected in both NB samples and S-type NB cell lines.Cyclopamine decreased the survival rate of the three cell lines while Shh increased it,and the inhibition effects of cyclopamine could be partially reversed by shh pre-treatment.Cyclopamine induced the cell apoptosis and the cell cycle arrest in G0/G1 phase,while Shh induced the reverse effects and could partially prevent effects of cyclopamine.Cyclopamine could also inhibit the growth of NB in vivo.Our studies revealed that activation of the Shh pathway is important for survival and proliferation of S-type NB cells in vivo and in vitro through affecting cell apoptosis and cell cycle,suggesting a new therapeutic approach to NB.

捷豹S-Type

你可以花60万元买到一款豪华轿车，但你未必就能因此收获到更多的魅力。一只充满灵性的飞豹划过长空，惊艳之中彰显绅士气质，这样的魅力，谁人可挡呢？

挑战“双B”美洲豹新鲜出击——Jaguar New S-Type 3.0

在迈入21世纪之始，Jaguar以S-Type作为首次跨入主管级豪华房车市场区区的开路先锋，当时汇集最尖端工艺技术、融合独特设计创意的S-Type，一推出便深受全球车坛的瞩目，同时也为TAGUAR揭开世纪交替的序幕，为日后上市的X-Type．全新XJ系列奠下良好的基础。转眼间。

2005款捷豹S-TYPE电子手刹故障

车型:2005款捷豹S-TYPE. 行驶里程:137750km. 故障现象:仪表上制动系统报警灯点亮,有时电子手刹无法释放. 故障诊断:接车后,验证故障现象,确如客户所述.使用SDD诊断仪进入系统读取故障码,发现在相关事件里面并没有相关的故障码. 执行建议选项,建议诊断驻车执行机构.点击确定以后,听到电机转动的声音,车辆手刹也可以正常的运作.以为只是一起偶发故障,在经过几次测试后均没问题,将车交与客户使用.

2003款美洲虎S-Type R轿车

在推出美洲虎S-Type轿车3年之后,美洲虎发布了全新设计的2003款S-Type R轿车。新款美洲虎S-Type R轿车在外型上没有做太大的改动,但为了显示其卓越的性能,对其内在结构进行了较大的改进。除了与车身同色的网状散热器护罩外。

绅士变身记 JAGUAR S-TYPE & JAGUAR XF

"犹记得戴安娜王妃初入皇室时,她驾驶着一辆经典的绿色S-Type,这款贵族座驾身形圆润、极富美感,不仅与王妃高贵气质相得益彰,也诠释着捷豹早期所崇尚的复古主义经典造型。跨入21世纪,捷豹全球设计总监严凯伦先生以一款风尚魅力的XF向世界昭告捷豹全新时代的到来,为我们演绎了一出精妙绝伦的绅士变身记。"

移动的古朴——试驾捷豹S-Type 3.0

一场热闹非凡的F1上海站赛事,让很多人领略了一级方程式赛车那V10发动机发出的刺耳狂嚣,记住了车神舒马赫那菲夷所思的冲出赛道、撞车、爆胎等意外,如此诸多的新闻及花絮中,一则捷豹车队将要退出F1赛事的新闻吸引了我的注意。

深水起重铺管船双铺管系统协同作业设计

在海底管道铺设项目中,需要S-lay型铺管系统和J-lay型铺管系统完成铺管任务。分别对两个系统进行合理的空间布置设计,将其集成于同一艘深水起重铺管船。对双铺管系统协同作业进行设计,使两个系统共享管段预制线、共享弃置与回收(Abandonment and Recovery,A&R)绞车系统,实现优势互补、提高铺管作业效率。

某型铺管船动力定位混合虚拟仿真系统开发

为增强铺管船动力定位操作仿真的真实性,提出一种将硬件在环仿真与虚拟仿真相结合的混合虚拟仿真方法。在此基础上,开发一种某型铺管船动力定位混合虚拟仿真系统,并使用混合虚拟仿真方法对动力定位操作进行仿真。结果表明:该系统可在三维虚拟场景中有效模拟船舶在海洋中的运动,混合虚拟仿真方法是可行的。研究成果可为船舶仿真系统开发提供一定参考。

海底管道铺设的力学分析

海底管道铺设是近海工程建设中的重要环节．由于铺管问题的控制方程具有较强的非线性，加之边界可动，所以问题的求解比较困难．本文采用奇异摄动法较好地解决了这一问题，并与有限元解作了比较．

深水管道S型铺设监测研究

目前,深水管道S型铺设取得了优异的铺设成果,逐渐成为深水海底管道铺设的重要方法,但其铺设环境复杂,上弯段管道与托管架动态接触,现有分析手段无法有效模拟。同时,S型铺设关键设备的设计技术还不成熟,没有相应的设计规范。首先对深水管道S型铺设开展监测研究,提出了深水管道S型铺监测方案,最后利用实验室模型实验验证了该方案的可行性,可以为深水S型铺设关键设备的设计提供必要的设计数据。

国内外铺管船的对比研究

通过对比国内外正在使用中的深／浅水铺管船，介绍了国内外海管铺设的方法。也分析了各个铺管船的作业能力、功能参数，指出了我国在海管铺设技术上的差距以及今后工作的方向。

铺管船锚泊定位系统优化设计研究

分析了铺管船锚泊定位系统的结构和它在海洋中的受力情况,研究了锚索悬链状态的特性。提出了锚泊定位系统的二次规划优化算法。目标函数定义为受力锚索的拉力的两两水平力差的平方和,受到线性等式或不等式锚索拉力的约束。求解采用Lagrange乘子法或凸二次规划的互补主元法。用面向对象的VC++工具开发了锚泊定位系统软件。对某铺管船的锚泊定位系统实例进行优化计算,得到满足环境力条件下锚索所承受的力和锚点位置,达到了设计要求。

深水铺管船用张紧器液压夹紧系统建模与仿真

综合考虑3000m水深铺管船用张紧器的工作过程及参数要求,设计液压夹紧系统为管线提供夹紧力并确定液压系统元件参数。利用AMESim软件建立仿真模型,并对液压缸快进、工进、保压、快退四个工作阶段及同步性进行仿真分析,除快进阶段液压缸存在速度波动外,其余三个阶段运行平稳;采用PID控制使液压缸具有良好的同步性,并通过分析液压缸动态特性,对液压系统进行优化。仿真结果表明液压夹紧系统基本满足张紧器工作要求。