Ginseng planting in Northeast China brings economic benefits but affects forest landscape integrity and native ecological processes.In order to quantify the impacts of ginseng planting on the forest landscape,Fusong C...Ginseng planting in Northeast China brings economic benefits but affects forest landscape integrity and native ecological processes.In order to quantify the impacts of ginseng planting on the forest landscape,Fusong County in Jilin Province was selected as a study area.The number and distribution of ginseng fields over different time was quantified based on remote sensing and ground surveys.Grid analysis and multiple regression analysis were used to study the impacts of ginseng planting on the landscape.The results showed that altitudes and slopes of ginseng fields increased and became increasingly scattered and smaller closer to the Changbai Mountain Nature Reserve.Ginseng fields and abandoned fields increased total patches and total area of the local forested landscape,and shared edge lengths between ginseng fields and forests,resulting in continuous fragmentation of the landscape.Although the total area of existing and abandoned ginseng fields accounts for a small fraction of the total landscape,their negative impacts on ecosystem conservation is significant.The local government needs to rationally plan ginseng planting,scientifically implement the restoration of abandoned ginseng lands,and enhance awareness of ginseng farmers to environmental stewardship.Our study has important significance for maintaining the healthy and stable development of the local ginseng industry and for improving the quality of regional ecological environment.展开更多
A cell suspension culture of Panax ginseng which may be continuously subcultured has been established. Embryogenic callus derived from clutured young leaves was used to initiate the culture.Plant growth regulators, ba...A cell suspension culture of Panax ginseng which may be continuously subcultured has been established. Embryogenic callus derived from clutured young leaves was used to initiate the culture.Plant growth regulators, basal medium formula and carbohydrate levels were examined to determine their various effects on suspension culture cell growth and development. The best selection of plant growth regulator, hasal medium and carbohydrate level is 2 mg / L 2,4-D: 0.5 mg / L KT,MS and 3% sucrose respectively.展开更多
Currently, transforming the mode of forest management and developing multiple forest management practices are actively encouraged in China. As one forest management type, ginseng cultivation under larch plantations ha...Currently, transforming the mode of forest management and developing multiple forest management practices are actively encouraged in China. As one forest management type, ginseng cultivation under larch plantations has been developed significantly in the east of Liaoning Province. However, research on the influence of the ecological environment for this mode of production is still deficient. Based on this, our study compares the plant diversity and soil properties in the ginseng cultivation under larch plantations(LG) with larch plantations(LP) and natural secondary forests(SF). First, we randomly selected three plots for each of the three stand types which have similar stand characteristics; then, we carried out a plant diversity survey and soil sampling in each of the nine plots. The results show that no significant difference was found in plant diversity between LG and LP, but theevenness of herbs was significantly lower in LG than LP. No obvious changes in soil physical properties were found in LG, but a significant decrease in most of the soil nutrient content was presented in LG. Furthermore, we found a correlation between plant diversity(H') and soil properties in the three kinds of stand types, especially between herbaceous plant diversity and soil properties. We conclude that ginseng cultivation under larch plantations has no obvious effect on plant diversity, except the herbaceous evenness. Soil fertility can be depleted significantly in LG, but physical structures are not affected. Moreover, maintaining the diversity of herbaceous plants and controlling the density of ginseng cultivation in LG by farmers are important for the ecological environment. Based on this study and its good comprehensive benefits and with the support of policy, we think this forest management type should be promoted moderately in the region.展开更多
This review article describes the research progresses on the control of ginseng gray mold, summarizes domestic and international related experimental re-sults and literature data using chemical fungicide, plant extrac...This review article describes the research progresses on the control of ginseng gray mold, summarizes domestic and international related experimental re-sults and literature data using chemical fungicide, plant extracts, microbial prepara-tion and antagonistic bacteria to control ginseng gray mold, and final y puts forward the existing problem and future research direction of the treatment and control of ginseng gray mold.展开更多
American ginseng, Panax quinquefolius L., is an herbaceous perennial species that is destructively harvested for its bioactive compounds called ginsenosides. The demand for this herb fosters illegal poaching and over-...American ginseng, Panax quinquefolius L., is an herbaceous perennial species that is destructively harvested for its bioactive compounds called ginsenosides. The demand for this herb fosters illegal poaching and over-harvesting that reduces genetic variability and population viability. Five wild populations in western North Carolina were studied to better understand the production of ginsenosides in leaf and root tissues. Total ginsenoside concentration was significantly higher in leaves than roots, though total yield was higher in roots due to greater root biomass. However, some ginsensosides (Rb2, Rd and Re) had higher or more consistent yields in leaves than roots, so might be developed into a sustainable source of these medicinally-active compounds. Additionally, we identified regional root chemotypes that differed in the production of the ginsenosides Rg1 and Re and could be developed into regional cultivars depending on the desired panel of ginsenosides.展开更多
The human interferon α 2b（hIFN-α2b） gene was cloned into binary vector pBI121 to obtain plant expression vector pBIFN. The recombinant plasmid pBIFN was transferred into Agrobacterium tumefaciens strain LBA4404. T...The human interferon α 2b（hIFN-α2b） gene was cloned into binary vector pBI121 to obtain plant expression vector pBIFN. The recombinant plasmid pBIFN was transferred into Agrobacterium tumefaciens strain LBA4404. Then the hIFN-α2b gene was introduced into ginseng callus cells via Agrobacterium-mediated transformation and the ginseng cell line carrying hIFN-α2b gene was selected on G418-containing medium. The presence of target gene in transformed cells was confirmed by PCR and RT-PCR. The results indicate that hIFN-α2b gene has been integrated into the ginseng cells＇ genome, with transcription products, hIFN-α2b expressed by the transgenic ginseng cells was detected by Western blot. It was shown that a specific protein band at 19000 could be observed. Cytopathic effect（CPE） inhibition assay using the W1SH-VSV system shows that the mean antiviral activity of expressed hlFN-a2α was 6.0× 10^4 IU/mL.展开更多
基金funded by National Key Research and Development Program of China(No.2016YFC0503603)。
文摘Ginseng planting in Northeast China brings economic benefits but affects forest landscape integrity and native ecological processes.In order to quantify the impacts of ginseng planting on the forest landscape,Fusong County in Jilin Province was selected as a study area.The number and distribution of ginseng fields over different time was quantified based on remote sensing and ground surveys.Grid analysis and multiple regression analysis were used to study the impacts of ginseng planting on the landscape.The results showed that altitudes and slopes of ginseng fields increased and became increasingly scattered and smaller closer to the Changbai Mountain Nature Reserve.Ginseng fields and abandoned fields increased total patches and total area of the local forested landscape,and shared edge lengths between ginseng fields and forests,resulting in continuous fragmentation of the landscape.Although the total area of existing and abandoned ginseng fields accounts for a small fraction of the total landscape,their negative impacts on ecosystem conservation is significant.The local government needs to rationally plan ginseng planting,scientifically implement the restoration of abandoned ginseng lands,and enhance awareness of ginseng farmers to environmental stewardship.Our study has important significance for maintaining the healthy and stable development of the local ginseng industry and for improving the quality of regional ecological environment.
文摘A cell suspension culture of Panax ginseng which may be continuously subcultured has been established. Embryogenic callus derived from clutured young leaves was used to initiate the culture.Plant growth regulators, basal medium formula and carbohydrate levels were examined to determine their various effects on suspension culture cell growth and development. The best selection of plant growth regulator, hasal medium and carbohydrate level is 2 mg / L 2,4-D: 0.5 mg / L KT,MS and 3% sucrose respectively.
基金financially supported by the research on soft science of forestry(2014-R05)consulting project of the Chinese Academy of Engineering(2013-XZ-22)
文摘Currently, transforming the mode of forest management and developing multiple forest management practices are actively encouraged in China. As one forest management type, ginseng cultivation under larch plantations has been developed significantly in the east of Liaoning Province. However, research on the influence of the ecological environment for this mode of production is still deficient. Based on this, our study compares the plant diversity and soil properties in the ginseng cultivation under larch plantations(LG) with larch plantations(LP) and natural secondary forests(SF). First, we randomly selected three plots for each of the three stand types which have similar stand characteristics; then, we carried out a plant diversity survey and soil sampling in each of the nine plots. The results show that no significant difference was found in plant diversity between LG and LP, but theevenness of herbs was significantly lower in LG than LP. No obvious changes in soil physical properties were found in LG, but a significant decrease in most of the soil nutrient content was presented in LG. Furthermore, we found a correlation between plant diversity(H') and soil properties in the three kinds of stand types, especially between herbaceous plant diversity and soil properties. We conclude that ginseng cultivation under larch plantations has no obvious effect on plant diversity, except the herbaceous evenness. Soil fertility can be depleted significantly in LG, but physical structures are not affected. Moreover, maintaining the diversity of herbaceous plants and controlling the density of ginseng cultivation in LG by farmers are important for the ecological environment. Based on this study and its good comprehensive benefits and with the support of policy, we think this forest management type should be promoted moderately in the region.
基金Supported by National Natural Science Fondation of China(31260067)Collegeenterprise Cooperation Project of Yanbian University[(2015)6]~~
文摘This review article describes the research progresses on the control of ginseng gray mold, summarizes domestic and international related experimental re-sults and literature data using chemical fungicide, plant extracts, microbial prepara-tion and antagonistic bacteria to control ginseng gray mold, and final y puts forward the existing problem and future research direction of the treatment and control of ginseng gray mold.
文摘American ginseng, Panax quinquefolius L., is an herbaceous perennial species that is destructively harvested for its bioactive compounds called ginsenosides. The demand for this herb fosters illegal poaching and over-harvesting that reduces genetic variability and population viability. Five wild populations in western North Carolina were studied to better understand the production of ginsenosides in leaf and root tissues. Total ginsenoside concentration was significantly higher in leaves than roots, though total yield was higher in roots due to greater root biomass. However, some ginsensosides (Rb2, Rd and Re) had higher or more consistent yields in leaves than roots, so might be developed into a sustainable source of these medicinally-active compounds. Additionally, we identified regional root chemotypes that differed in the production of the ginsenosides Rg1 and Re and could be developed into regional cultivars depending on the desired panel of ginsenosides.
基金Supported by the Science and Technology Development Planning Foundation of Jilin Province of China(No.20030405)
文摘The human interferon α 2b（hIFN-α2b） gene was cloned into binary vector pBI121 to obtain plant expression vector pBIFN. The recombinant plasmid pBIFN was transferred into Agrobacterium tumefaciens strain LBA4404. Then the hIFN-α2b gene was introduced into ginseng callus cells via Agrobacterium-mediated transformation and the ginseng cell line carrying hIFN-α2b gene was selected on G418-containing medium. The presence of target gene in transformed cells was confirmed by PCR and RT-PCR. The results indicate that hIFN-α2b gene has been integrated into the ginseng cells＇ genome, with transcription products, hIFN-α2b expressed by the transgenic ginseng cells was detected by Western blot. It was shown that a specific protein band at 19000 could be observed. Cytopathic effect（CPE） inhibition assay using the W1SH-VSV system shows that the mean antiviral activity of expressed hlFN-a2α was 6.0× 10^4 IU/mL.
