AIM: To construct the natural immune Fab antibody phage display libraries of colorectal cancer and to select antibodies related with colorectal cancer. METHODS: Extract total RNA from tissue of local cancer metastasis...AIM: To construct the natural immune Fab antibody phage display libraries of colorectal cancer and to select antibodies related with colorectal cancer. METHODS: Extract total RNA from tissue of local cancer metastasis lymph nodes of patients with colorectal cancer. RT-PCR was used to amplify the heavy chain Fd and light chain kappa and the amplification products were inserted successively into the vector pComb3 to construct the human libraries of Fab antibodies. They were then panned by phage display technology. By means of Dot immunoblotting and ELISA, the libraries were identified and the Fab phage antibodies binding with antigens of colorectal cancer were selected. RESULTS: The amplified fragments of Fd and kappa gained by RT-PCR were about 650 bp. Fd and kappa PCR products were subsequently inserted into the vector pComb3, resulting in a recombination rate of 40% and the volume of Fab phage display library reached 1.48 x 10(6).The libraries were enriched about 120-fold by 3 cycles of adsorption-elution-multiplication (panning). Dot immunoblotting showed Fab expressions on the phage libraries and ELISA showed 5 clones of Fab phage antibodies which had binding activities with antigens of colorectal cancer. CONCLUSION: The natural immune Fab antibody phage display libraries of colorectal cancer were constructed. They could be used to select the relative antibodies of colorectal cancer.展开更多
吉时利仪器公司是为不断增长的测量需求提供解决方案的领导者，近日发布了美国AMD公司选用吉时利（Keithley）公司S680型直流／射频（DC／RF）半导体参数自动测试系统，以支持AMD公司位于德国德累斯顿市技术发展水平最高的300mm Fab 36...吉时利仪器公司是为不断增长的测量需求提供解决方案的领导者，近日发布了美国AMD公司选用吉时利（Keithley）公司S680型直流／射频（DC／RF）半导体参数自动测试系统，以支持AMD公司位于德国德累斯顿市技术发展水平最高的300mm Fab 36公司的先进逻辑芯片的全部生产。S680直流／射频（DC／RF）系统的优点包括先进的测量能力、较低的保有成本、很高的产量以及强有力的本地服务和技术支持。展开更多
吉时利仪器公司今日发布了美国AMD公司选用吉时利(Keithley）公司S680型直流/射频（DC/RF）半导体参数自动测试系统，以支持AMD公司位于德国德累斯顿市技术发展水平最高的300mm Fab 36的先进逻辑芯片的全部生产。S680直流/射频（DC/RF...吉时利仪器公司今日发布了美国AMD公司选用吉时利(Keithley）公司S680型直流/射频（DC/RF）半导体参数自动测试系统，以支持AMD公司位于德国德累斯顿市技术发展水平最高的300mm Fab 36的先进逻辑芯片的全部生产。S680直流/射频（DC/RF）系统的优点包括先进的测量能力、较低的保有成本、很高的产量以及强有力的本地服务和技术支持。展开更多
AIM:To labed Anti-hepatoma monoclonal antibody(mAb) fragment HAb18 F(ab')_2 was labeled with 188 Re for the pharmacokinetic model of ^(188)Re-HAb18 F(ab')_2 and to evaluate its pharmacokinetic parameters in he...AIM:To labed Anti-hepatoma monoclonal antibody(mAb) fragment HAb18 F(ab')_2 was labeled with 188 Re for the pharmacokinetic model of ^(188)Re-HAb18 F(ab')_2 and to evaluate its pharmacokinetic parameters in hepatoma- bearing nude mice. METHODS:HAb18 F(ab')_2 was directly labeled with ^(188)Re using 2-mercaptoethanol(2-ME)as reducing agents. Labeling efficiency and immunoreactivity of ^(188)Re-HAb18 F (ab')_2 were evaluated by Whatman 3MM paper chromatography and live cell assay,respectively. Biodistribution analysis was also conducted in nude mice bearing human hepatoma in which animals were sacrificed at different time points(1,4,18,24 and 24h)after ^(188)Re-HAb18 F(ab')_2 was injected through tail-vein into hepatoma-bearing nude mice.The blood and radioactivity of organs and mass were measured.The concentrations of ^(188)Re-HAb18 F(ab')_2 were evaluated with a pharrnacokinetic 3P97 software. RESULTS:The optimum labeling efficiency and immunoreactive fraction were 91.7% and 0.78%, respectively.The parameters of ^(188)Re-HAb18 F(ab')_2 were: T_(1/2),2.29h;Vd,1.49×10^(-9)L·Bq^(-1);AUC,20.49×10~9Bq·h· L^(-1);CL,0.45×10^(-3)L·h^(-1).^(188)Re-HAb18 F(ab')_2 could locate specially in hepatoma with high selective reactivity of HAb18 F(ab')_2.^(188)Re-HAbl8 F(ab')_2 was mainly eliminated by kidney.The maximal tumor to blood ratio was at 48h,and maximal tumor to liver ratio was at 18h. CONCLUTION:The pharmacokinetics of ^(188)Re-HAb18 F(ab')_2 fit a I-compartment model.^(188)Re-HAb18 F(ab')_2 can be uptaken selectively at the hepatoma site.展开更多
吉时利仪器公司日前宣布，美国AMD公司选用吉时利(Keithley)公司S680型直流／射频(DC/RF)半导体参数自动测试系统，以支持位于德国的300mm Fab 36的先进逻辑芯片的全部生产。S680 DC/RF系统的优点包括先进的测量能力、较低的保有成本、...吉时利仪器公司日前宣布，美国AMD公司选用吉时利(Keithley)公司S680型直流／射频(DC/RF)半导体参数自动测试系统，以支持位于德国的300mm Fab 36的先进逻辑芯片的全部生产。S680 DC/RF系统的优点包括先进的测量能力、较低的保有成本、很高的产量以及强有力的本地服务和技术支持。展开更多
本试验采用5 s rDNA间隔序列分析和SRAP标记验证以色列野生二粒小麦与光稃野燕麦远缘杂种的真实性。结果表明,引物对PⅠ/PⅡ在以色列野生二粒小麦与光稃野燕麦杂种中扩增出了父本特异带,父本的遗传物质导入了小麦基因组。经SRAP标记分析...本试验采用5 s rDNA间隔序列分析和SRAP标记验证以色列野生二粒小麦与光稃野燕麦远缘杂种的真实性。结果表明,引物对PⅠ/PⅡ在以色列野生二粒小麦与光稃野燕麦杂种中扩增出了父本特异带,父本的遗传物质导入了小麦基因组。经SRAP标记分析,引物对me 3-em3在该杂种中也扩增出了父本特异带,进一步证明了杂种的真实性。展开更多
基金Supported by the Natural Science Foundation of Guangdong Province,China, No. 980120the Foundation of Excellent Youth Teacher,China,2001
文摘AIM: To construct the natural immune Fab antibody phage display libraries of colorectal cancer and to select antibodies related with colorectal cancer. METHODS: Extract total RNA from tissue of local cancer metastasis lymph nodes of patients with colorectal cancer. RT-PCR was used to amplify the heavy chain Fd and light chain kappa and the amplification products were inserted successively into the vector pComb3 to construct the human libraries of Fab antibodies. They were then panned by phage display technology. By means of Dot immunoblotting and ELISA, the libraries were identified and the Fab phage antibodies binding with antigens of colorectal cancer were selected. RESULTS: The amplified fragments of Fd and kappa gained by RT-PCR were about 650 bp. Fd and kappa PCR products were subsequently inserted into the vector pComb3, resulting in a recombination rate of 40% and the volume of Fab phage display library reached 1.48 x 10(6).The libraries were enriched about 120-fold by 3 cycles of adsorption-elution-multiplication (panning). Dot immunoblotting showed Fab expressions on the phage libraries and ELISA showed 5 clones of Fab phage antibodies which had binding activities with antigens of colorectal cancer. CONCLUSION: The natural immune Fab antibody phage display libraries of colorectal cancer were constructed. They could be used to select the relative antibodies of colorectal cancer.
文摘吉时利仪器公司是为不断增长的测量需求提供解决方案的领导者，近日发布了美国AMD公司选用吉时利（Keithley）公司S680型直流／射频（DC／RF）半导体参数自动测试系统，以支持AMD公司位于德国德累斯顿市技术发展水平最高的300mm Fab 36公司的先进逻辑芯片的全部生产。S680直流／射频（DC／RF）系统的优点包括先进的测量能力、较低的保有成本、很高的产量以及强有力的本地服务和技术支持。
文摘吉时利仪器公司今日发布了美国AMD公司选用吉时利(Keithley）公司S680型直流/射频（DC/RF）半导体参数自动测试系统，以支持AMD公司位于德国德累斯顿市技术发展水平最高的300mm Fab 36的先进逻辑芯片的全部生产。S680直流/射频（DC/RF）系统的优点包括先进的测量能力、较低的保有成本、很高的产量以及强有力的本地服务和技术支持。
基金the National Natural Science Foundation of China,No.39700175
文摘AIM:To labed Anti-hepatoma monoclonal antibody(mAb) fragment HAb18 F(ab')_2 was labeled with 188 Re for the pharmacokinetic model of ^(188)Re-HAb18 F(ab')_2 and to evaluate its pharmacokinetic parameters in hepatoma- bearing nude mice. METHODS:HAb18 F(ab')_2 was directly labeled with ^(188)Re using 2-mercaptoethanol(2-ME)as reducing agents. Labeling efficiency and immunoreactivity of ^(188)Re-HAb18 F (ab')_2 were evaluated by Whatman 3MM paper chromatography and live cell assay,respectively. Biodistribution analysis was also conducted in nude mice bearing human hepatoma in which animals were sacrificed at different time points(1,4,18,24 and 24h)after ^(188)Re-HAb18 F(ab')_2 was injected through tail-vein into hepatoma-bearing nude mice.The blood and radioactivity of organs and mass were measured.The concentrations of ^(188)Re-HAb18 F(ab')_2 were evaluated with a pharrnacokinetic 3P97 software. RESULTS:The optimum labeling efficiency and immunoreactive fraction were 91.7% and 0.78%, respectively.The parameters of ^(188)Re-HAb18 F(ab')_2 were: T_(1/2),2.29h;Vd,1.49×10^(-9)L·Bq^(-1);AUC,20.49×10~9Bq·h· L^(-1);CL,0.45×10^(-3)L·h^(-1).^(188)Re-HAb18 F(ab')_2 could locate specially in hepatoma with high selective reactivity of HAb18 F(ab')_2.^(188)Re-HAbl8 F(ab')_2 was mainly eliminated by kidney.The maximal tumor to blood ratio was at 48h,and maximal tumor to liver ratio was at 18h. CONCLUTION:The pharmacokinetics of ^(188)Re-HAb18 F(ab')_2 fit a I-compartment model.^(188)Re-HAb18 F(ab')_2 can be uptaken selectively at the hepatoma site.
文摘吉时利仪器公司日前宣布，美国AMD公司选用吉时利(Keithley)公司S680型直流／射频(DC/RF)半导体参数自动测试系统，以支持位于德国的300mm Fab 36的先进逻辑芯片的全部生产。S680 DC/RF系统的优点包括先进的测量能力、较低的保有成本、很高的产量以及强有力的本地服务和技术支持。
文摘本试验采用5 s rDNA间隔序列分析和SRAP标记验证以色列野生二粒小麦与光稃野燕麦远缘杂种的真实性。结果表明,引物对PⅠ/PⅡ在以色列野生二粒小麦与光稃野燕麦杂种中扩增出了父本特异带,父本的遗传物质导入了小麦基因组。经SRAP标记分析,引物对me 3-em3在该杂种中也扩增出了父本特异带,进一步证明了杂种的真实性。
