To illuminate the similarities and differences between wild and cultivated Sarcandra glabra(S.glabra),we performed a comprehensively study on 26 batches of cultivated S.glabra and 2 batches of wild S.glabra.Chemical c...To illuminate the similarities and differences between wild and cultivated Sarcandra glabra(S.glabra),we performed a comprehensively study on 26 batches of cultivated S.glabra and 2 batches of wild S.glabra.Chemical constituents and distribution characteristics of roots,stems and leaves in both wild and cultivated S.glabra were investigated through UHPLC-TOF-MS method.The result revealed that there were significant differences between roots,stems and leaves in S.glabra.And the chemical contents in the root part were less or even absence than those in leaf and stem,which suggested the root organ could be excluded as medicine.Meanwhile,the chemical contents of stems and leaves in cultivated S.glabra was sightly higher than that of wild samples.Therefore,cultivated S.glabra may have a high potential for substitution of wild S.glabra without affecting its pharmaceutical properties.In summary,our study could provide important information to the molecular basis for quality control of S.glabra.展开更多
目的采用18 S rRNA基因、ITS2序列、SCAR标记等3种DNA分子标记法鉴别草珊瑚,为其分子鉴定提供依据。方法通过PCR扩增、克隆测序后获得草珊瑚18 S rRNA基因序列,并进行Blast比对;通过PCR扩增、测序并注释后获得草珊瑚ITS2序列,从GenBank...目的采用18 S rRNA基因、ITS2序列、SCAR标记等3种DNA分子标记法鉴别草珊瑚,为其分子鉴定提供依据。方法通过PCR扩增、克隆测序后获得草珊瑚18 S rRNA基因序列,并进行Blast比对;通过PCR扩增、测序并注释后获得草珊瑚ITS2序列,从GenBank上收集混伪品和其他植物的ITS2序列,使用MEGA5.5软件,计算种内种间遗传距离,构建系统聚类树;通过RAPD法获得草珊瑚SCAR分子标记,克隆测序后,设计特异性引物扩增草珊瑚及其混伪品。结果获得的草珊瑚18 S rRNA基因长度为1820 bp,Blast比对显示草珊瑚与金粟兰科同源性最高,同源性为99%,证明其为草珊瑚的18 S rRNA基因。获得的草珊瑚ITS2序列长度为500 bp,草珊瑚与其混伪品之间的遗传距离为0.190~0.219,混伪品之间的遗传距离为0~0.074,聚类分析显示草珊瑚聚为一支,混伪品聚为一支,与其他植物距离较远。获得草珊瑚的SCAR分子标记,用特异性引物扩增出现草珊瑚特异性产物,混伪品未出现特异性产物。结论3种分子标记法联合可更有效地鉴别草珊瑚及其混伪品,从而建立一套新的鉴别草珊瑚与混伪品的方法,为其鉴别提供新的思路。展开更多
基金the Science and Technology Development Fund,Macao SAR(No.0023/2019/AKP)Guangxi Science and Technology Department Fund(No.AD17195002)。
文摘To illuminate the similarities and differences between wild and cultivated Sarcandra glabra(S.glabra),we performed a comprehensively study on 26 batches of cultivated S.glabra and 2 batches of wild S.glabra.Chemical constituents and distribution characteristics of roots,stems and leaves in both wild and cultivated S.glabra were investigated through UHPLC-TOF-MS method.The result revealed that there were significant differences between roots,stems and leaves in S.glabra.And the chemical contents in the root part were less or even absence than those in leaf and stem,which suggested the root organ could be excluded as medicine.Meanwhile,the chemical contents of stems and leaves in cultivated S.glabra was sightly higher than that of wild samples.Therefore,cultivated S.glabra may have a high potential for substitution of wild S.glabra without affecting its pharmaceutical properties.In summary,our study could provide important information to the molecular basis for quality control of S.glabra.
文摘目的采用18 S rRNA基因、ITS2序列、SCAR标记等3种DNA分子标记法鉴别草珊瑚,为其分子鉴定提供依据。方法通过PCR扩增、克隆测序后获得草珊瑚18 S rRNA基因序列,并进行Blast比对;通过PCR扩增、测序并注释后获得草珊瑚ITS2序列,从GenBank上收集混伪品和其他植物的ITS2序列,使用MEGA5.5软件,计算种内种间遗传距离,构建系统聚类树;通过RAPD法获得草珊瑚SCAR分子标记,克隆测序后,设计特异性引物扩增草珊瑚及其混伪品。结果获得的草珊瑚18 S rRNA基因长度为1820 bp,Blast比对显示草珊瑚与金粟兰科同源性最高,同源性为99%,证明其为草珊瑚的18 S rRNA基因。获得的草珊瑚ITS2序列长度为500 bp,草珊瑚与其混伪品之间的遗传距离为0.190~0.219,混伪品之间的遗传距离为0~0.074,聚类分析显示草珊瑚聚为一支,混伪品聚为一支,与其他植物距离较远。获得草珊瑚的SCAR分子标记,用特异性引物扩增出现草珊瑚特异性产物,混伪品未出现特异性产物。结论3种分子标记法联合可更有效地鉴别草珊瑚及其混伪品,从而建立一套新的鉴别草珊瑚与混伪品的方法,为其鉴别提供新的思路。
