Chlorpyrifos （CPF） is a broad-spectrum organophosphate insecticide. Glu- tathione S-transferases （GSTs） in insects are a family of detoxification enzymes and they play critical roles in CPF detoxification. Spodopt...Chlorpyrifos （CPF） is a broad-spectrum organophosphate insecticide. Glu- tathione S-transferases （GSTs） in insects are a family of detoxification enzymes and they play critical roles in CPF detoxification. Spodoptera litura is one of the most destructive agricultural pests in tropical and subtropical areas in the world. In this study, 37 Slgsts from 46 unique transcripts of gsts in S. litura transcriptome data, including eight pre- viously reported GSTs, were identified and their expression patterns in susceptible and 12-generation-CPF-treated strains were analyzed to understand the roles of these Slgsts in sublethal doses of CPF tolerance. The results indicate that the members of the S. litura GST superfamily could be distinguished into three major groups： one group, includ- ing six cytosolic Slgsts （SIGSTel, SIGSTe3, SIGSTelO, SIGSTe15, SIGSTo2 and SIGSTs5） and two microsomal Slgsts （SIMGSTI-2 and SIMGST1-3）, was directly responsible for CPF induction in both 12-generation-treated and susceptible strains; the second group, including three cytosolic Slgsts （SlGSTe13, SIGSTtl and SIGSTzl） and one microsomal Slgst （SIMGSTI-1）, was induced only in the 12-generation-treated strain; the third group, including eight cytosolic Slgsts （two epsilon, three delta, one omega, one zeta and one un- classified Slgst）, was expressed 1.52-5.15-fold higher in the 12-generation-treated strain than in the susceptible strain.展开更多
Nickel accumulated in midugt of Spodoptera litura Fabricius could induce the expression of metal-lothionein,one of the most important detoxification proteins in organisms.In the present study,the effects of dietary ni...Nickel accumulated in midugt of Spodoptera litura Fabricius could induce the expression of metal-lothionein,one of the most important detoxification proteins in organisms.In the present study,the effects of dietary nickel on the activities of detoxification enzymes,such as carboxylesterase(CarE) and glutathione S-transferase(GST) in the midgut of S.litura larvae have been studied to get an un-derstanding of the detoxification mechanisms of S.litura larvae to excessive nickel.Results showed that CarE activities in the midgut of the 5th instar larvae decreased at lower levels of nickel(≤5 mg/kg),while increased with increasing nickel doses at higher levels of nickel(≥10 mg/kg) exposure in suc-cessive 3 generations.CarE activities of the 6th instar larvae were also characterized as inhibited at low levels of nickel exposure,and improved at higher levels in the 1st generation.CarE activities of 6th instar larvae in the 2nd and 3rd generations were all lower than that in control.However,GST activities in the midgut of the 5th and 6th instar larvae all increased with increasing nickel doses(1―20 mg/kg) in diets.展开更多
文摘Chlorpyrifos （CPF） is a broad-spectrum organophosphate insecticide. Glu- tathione S-transferases （GSTs） in insects are a family of detoxification enzymes and they play critical roles in CPF detoxification. Spodoptera litura is one of the most destructive agricultural pests in tropical and subtropical areas in the world. In this study, 37 Slgsts from 46 unique transcripts of gsts in S. litura transcriptome data, including eight pre- viously reported GSTs, were identified and their expression patterns in susceptible and 12-generation-CPF-treated strains were analyzed to understand the roles of these Slgsts in sublethal doses of CPF tolerance. The results indicate that the members of the S. litura GST superfamily could be distinguished into three major groups： one group, includ- ing six cytosolic Slgsts （SIGSTel, SIGSTe3, SIGSTelO, SIGSTe15, SIGSTo2 and SIGSTs5） and two microsomal Slgsts （SIMGSTI-2 and SIMGST1-3）, was directly responsible for CPF induction in both 12-generation-treated and susceptible strains; the second group, including three cytosolic Slgsts （SlGSTe13, SIGSTtl and SIGSTzl） and one microsomal Slgst （SIMGSTI-1）, was induced only in the 12-generation-treated strain; the third group, including eight cytosolic Slgsts （two epsilon, three delta, one omega, one zeta and one un- classified Slgst）, was expressed 1.52-5.15-fold higher in the 12-generation-treated strain than in the susceptible strain.
基金the National Basic Research Development Program of China (Grant No. 2006CB102001)National Natural Science Foundation of China (Grant No. 30771458)+1 种基金Doctoral Fundaytion of Ministry of Education of China (Grant No. 20070558029)Basic Research Group Programs of Guangdong Province (Grant No.E039254)
文摘Nickel accumulated in midugt of Spodoptera litura Fabricius could induce the expression of metal-lothionein,one of the most important detoxification proteins in organisms.In the present study,the effects of dietary nickel on the activities of detoxification enzymes,such as carboxylesterase(CarE) and glutathione S-transferase(GST) in the midgut of S.litura larvae have been studied to get an un-derstanding of the detoxification mechanisms of S.litura larvae to excessive nickel.Results showed that CarE activities in the midgut of the 5th instar larvae decreased at lower levels of nickel(≤5 mg/kg),while increased with increasing nickel doses at higher levels of nickel(≥10 mg/kg) exposure in suc-cessive 3 generations.CarE activities of the 6th instar larvae were also characterized as inhibited at low levels of nickel exposure,and improved at higher levels in the 1st generation.CarE activities of 6th instar larvae in the 2nd and 3rd generations were all lower than that in control.However,GST activities in the midgut of the 5th and 6th instar larvae all increased with increasing nickel doses(1―20 mg/kg) in diets.
