The tested strain from typical clinical cases,and the physio-chemical characteristics,16 S rRNA gene sequence and drug sensitivity challenge property were analyzed.This isolate was determined as Streptococcus agalacti...The tested strain from typical clinical cases,and the physio-chemical characteristics,16 S rRNA gene sequence and drug sensitivity challenge property were analyzed.This isolate was determined as Streptococcus agalactiae,16 S rRNA gene fragment of 1 280 bp in length.Blast analysis showed that this fragment shared a 100% homology with the conservative region of Streptococcus agalactiae isolate（AB023574）,Streptococcus dysqalactiae isolate（HM146084.1,AB537922,EU075033）,Streptococcus difficilis isolate（AB112407） accessed in Genbank.The isolate was sensitive to ciprofloxacin,neomycin,spectinomycin,furadantin and enoxacin.The results lay foundation for the effective control,rational medication and vaccine preparation for Streptococcus disease on bullfrogs.展开更多
IgT(Immunoglobulin T)作为新型免疫球蛋白在粘膜免疫中具有重要作用.文中根据尼罗罗非鱼IgT基因序列构建原核表达载体获得IgT重组蛋白,相对分子量约75 k Da,并制备出效价较高的小鼠抗罗非鱼IgT的多克隆抗体.荧光定量PCR检测证明:健康...IgT(Immunoglobulin T)作为新型免疫球蛋白在粘膜免疫中具有重要作用.文中根据尼罗罗非鱼IgT基因序列构建原核表达载体获得IgT重组蛋白,相对分子量约75 k Da,并制备出效价较高的小鼠抗罗非鱼IgT的多克隆抗体.荧光定量PCR检测证明:健康尼罗罗非鱼中IgT在多种组织中都有表达,其中脾脏、皮肤、肠和鳃中表达量相对较高.无乳链球菌感染后,皮肤和头肾中IgT mRNA的相对表达量分别在12 h和4 d显著上调.免疫组化结果显示:IgT蛋白在头肾和皮肤中均有少量分布,在无乳链球菌刺激后,在皮肤中出现大量分布.以上结果表明:IgT在尼罗罗非鱼皮肤粘膜免疫中具有重要作用.展开更多
Pyelonephritis is an inflammation of the kidney tissue, calyces, and renal pelvis. It is commonly caused by bacterial infection that has spread up the urinary tract. Common bacteria are Escherichia coli and Enterococc...Pyelonephritis is an inflammation of the kidney tissue, calyces, and renal pelvis. It is commonly caused by bacterial infection that has spread up the urinary tract. Common bacteria are Escherichia coli and Enterococcus faecalis. Beta hemolytic streptococci also cause pyelonephritis. Beta hemolytic streptococcus such as Streptococcus agalactiae, and S. dysgalactiae subsp. equisimilis, when grown on blood-agar, produce complete hemolysis around each colony, usually cause severe infections including many cases of cellulitis, pneumonia, meningitis and sepsis. However the analysis of pyelonephritis causing beta hemolytic streptococcus has seldom been investigated. In this study, we tried to clarify the relationship between beta hemolytic streptococcus and pyelonephritis patient in Japan. We investigated beta hemolytic streptococcus isolated from pyelonephritis patient at two tertiary care hospitals in the central region of Japan by analyzing the background and antimicrobial susceptibility of beta hemolytic streptococcus. We recovered total sixteen beta hemolytic streptococcus in nine years. In pyelonephritis patients, beta hemolytic streptococcus was isolated from 9 S. agalactiae and 7 S. dysgalactiae subsp. equisimilis, respectively. The numbers of streptococci from female patients were more than those from male patients. Bacteria were not isolated from under 30 year patients. All S. dysgalactiae subsp. equisimilis were isolated from 60-year-age patients. About one fifth of beta hemolytic streptococcus had erythromycin and minocycline-resistant ability. Four beta hemolytic streptococci that were all S. agalactiae, had levofloxacin-resistant ability. Only one S. agalactiae had trimethoprim-sulfamethoxazole-resistant ability. Ampicillin was effective against all beta hemolytic streptococci. In our results, beta hemolytic streptococcus caused pyelonephritis in aged people and the quarter of these bacteria had some antibiotics resistance.展开更多
[ Objective ] This study aimed to identify Streptococcus agalactiae and lay the foundation for the prevention and control of mastiffs in dairy cows. [ Method] Ten strains were isolated from milk samples produced by di...[ Objective ] This study aimed to identify Streptococcus agalactiae and lay the foundation for the prevention and control of mastiffs in dairy cows. [ Method] Ten strains were isolated from milk samples produced by diseased dairy cows suffering from mastitis for morphologic observation, culture characteristic investigation, biochemical identification and Lancefield grouping. The isolated strains were identified at the molecular level by nested-PCR. [ Result] Among the ten isolates, six strains were 13-hemolytic and Gram-positive on blood agar, belonging to Lancefield group B, which were identified as Streptococcus agalactiae by biochemical identification and nested-PCR. After overnight incubation, the coincidence rate between results of nested-PCR detection and biochemical identification reached 100%. [ Conclusion ] Bacterial incubation, rapid DNA extraction and specific PCR can provide basis for early epidemiological survey of Streptococcus agalactiae infection in cattle.展开更多
基金Supported by Scientific and Technological Project of Huzhou City(2009GN03)Major Agricultural Program in Zhejiang Province(2009C12056)
文摘The tested strain from typical clinical cases,and the physio-chemical characteristics,16 S rRNA gene sequence and drug sensitivity challenge property were analyzed.This isolate was determined as Streptococcus agalactiae,16 S rRNA gene fragment of 1 280 bp in length.Blast analysis showed that this fragment shared a 100% homology with the conservative region of Streptococcus agalactiae isolate（AB023574）,Streptococcus dysqalactiae isolate（HM146084.1,AB537922,EU075033）,Streptococcus difficilis isolate（AB112407） accessed in Genbank.The isolate was sensitive to ciprofloxacin,neomycin,spectinomycin,furadantin and enoxacin.The results lay foundation for the effective control,rational medication and vaccine preparation for Streptococcus disease on bullfrogs.
文摘Pyelonephritis is an inflammation of the kidney tissue, calyces, and renal pelvis. It is commonly caused by bacterial infection that has spread up the urinary tract. Common bacteria are Escherichia coli and Enterococcus faecalis. Beta hemolytic streptococci also cause pyelonephritis. Beta hemolytic streptococcus such as Streptococcus agalactiae, and S. dysgalactiae subsp. equisimilis, when grown on blood-agar, produce complete hemolysis around each colony, usually cause severe infections including many cases of cellulitis, pneumonia, meningitis and sepsis. However the analysis of pyelonephritis causing beta hemolytic streptococcus has seldom been investigated. In this study, we tried to clarify the relationship between beta hemolytic streptococcus and pyelonephritis patient in Japan. We investigated beta hemolytic streptococcus isolated from pyelonephritis patient at two tertiary care hospitals in the central region of Japan by analyzing the background and antimicrobial susceptibility of beta hemolytic streptococcus. We recovered total sixteen beta hemolytic streptococcus in nine years. In pyelonephritis patients, beta hemolytic streptococcus was isolated from 9 S. agalactiae and 7 S. dysgalactiae subsp. equisimilis, respectively. The numbers of streptococci from female patients were more than those from male patients. Bacteria were not isolated from under 30 year patients. All S. dysgalactiae subsp. equisimilis were isolated from 60-year-age patients. About one fifth of beta hemolytic streptococcus had erythromycin and minocycline-resistant ability. Four beta hemolytic streptococci that were all S. agalactiae, had levofloxacin-resistant ability. Only one S. agalactiae had trimethoprim-sulfamethoxazole-resistant ability. Ampicillin was effective against all beta hemolytic streptococci. In our results, beta hemolytic streptococcus caused pyelonephritis in aged people and the quarter of these bacteria had some antibiotics resistance.
基金Supported by Science and Technology Development Project of Shijiazhuang City(08150132A)Spark Program of the Ministry of Science and Technology of China(2012GA6200025)+1 种基金Scientific Research Innovation Team of Hebei Normal University of Science and Technology(CXTD201201)Natural science Foundation of China(31072004)
文摘[ Objective ] This study aimed to identify Streptococcus agalactiae and lay the foundation for the prevention and control of mastiffs in dairy cows. [ Method] Ten strains were isolated from milk samples produced by diseased dairy cows suffering from mastitis for morphologic observation, culture characteristic investigation, biochemical identification and Lancefield grouping. The isolated strains were identified at the molecular level by nested-PCR. [ Result] Among the ten isolates, six strains were 13-hemolytic and Gram-positive on blood agar, belonging to Lancefield group B, which were identified as Streptococcus agalactiae by biochemical identification and nested-PCR. After overnight incubation, the coincidence rate between results of nested-PCR detection and biochemical identification reached 100%. [ Conclusion ] Bacterial incubation, rapid DNA extraction and specific PCR can provide basis for early epidemiological survey of Streptococcus agalactiae infection in cattle.
