Emerging evidence has suggested global histone H4 acetylation status plays an important role in neural plasticity. For instance, the imbalance of this epigenetic marker has been hypothesized as a key factor for the de...Emerging evidence has suggested global histone H4 acetylation status plays an important role in neural plasticity. For instance, the imbalance of this epigenetic marker has been hypothesized as a key factor for the development and progression of several neurological diseases. Likewise, astrocytic reactivity-a wellknown process that markedly influences the tissue remodeling after a central nervous system injury-is crucial for tissue remodeling after spinal cord injury（SCI）. However, the linkage between the above-mentioned mechanisms after SCI remains poorly understood. We sought to investigate the relation between both glial fibrillary acidic protein（GFAP） and S100 calcium-binding protein B（S100B）（astrocytic reactivity classical markers） and global histone H4 acetylation levels. Sixty-one male Wistar rats（aged ~3 months） were divided into the following groups： sham; 6 hours post-SCI; 24 hours post-SCI; 48 hours post-SCI; 72 hours post-SCI; and 7 days post-SCI. The results suggested that GFAP, but not S100B was associated with global histone H4 acetylation levels. Moreover, global histone H4 acetylation levels exhibited a complex pattern after SCI, encompassing at least three clearly defined phases（first phase： no changes in the 6, 24 and 48 hours post-SCI groups; second phase： increased levels in the 72 hours post-SCI group; and a third phase： return to levels similar to control in the 7 days post-SCI group）. Overall, these findings suggest global H4 acetylation levels exhibit distinct patterns of expression during the first week post-SCI, which may be associated with GFAP levels in the perilesional tissue. Current data encourage studies using H4 acetylation as a possible biomarker for tissue remodeling after spinal cord injury.展开更多
基金supported by Brazilian funding agencies CNPq,CAPES and FAPERGS
文摘Emerging evidence has suggested global histone H4 acetylation status plays an important role in neural plasticity. For instance, the imbalance of this epigenetic marker has been hypothesized as a key factor for the development and progression of several neurological diseases. Likewise, astrocytic reactivity-a wellknown process that markedly influences the tissue remodeling after a central nervous system injury-is crucial for tissue remodeling after spinal cord injury（SCI）. However, the linkage between the above-mentioned mechanisms after SCI remains poorly understood. We sought to investigate the relation between both glial fibrillary acidic protein（GFAP） and S100 calcium-binding protein B（S100B）（astrocytic reactivity classical markers） and global histone H4 acetylation levels. Sixty-one male Wistar rats（aged ~3 months） were divided into the following groups： sham; 6 hours post-SCI; 24 hours post-SCI; 48 hours post-SCI; 72 hours post-SCI; and 7 days post-SCI. The results suggested that GFAP, but not S100B was associated with global histone H4 acetylation levels. Moreover, global histone H4 acetylation levels exhibited a complex pattern after SCI, encompassing at least three clearly defined phases（first phase： no changes in the 6, 24 and 48 hours post-SCI groups; second phase： increased levels in the 72 hours post-SCI group; and a third phase： return to levels similar to control in the 7 days post-SCI group）. Overall, these findings suggest global H4 acetylation levels exhibit distinct patterns of expression during the first week post-SCI, which may be associated with GFAP levels in the perilesional tissue. Current data encourage studies using H4 acetylation as a possible biomarker for tissue remodeling after spinal cord injury.
文摘目的分析头部CT血管成像(computed tomography angiography,CTA)联合血清胶质纤维酸性蛋白(glial fibrillary acidic protein,GFAP)、S100钙结合蛋白B(S100 calcium-binding protein B,S100B)在急性脑缺血(acute cerebral ischemia,ACI)诊断中的应用价值。方法收集2022年1月~2022年12月在哈尔滨医科大学附属第一医院治疗的ACI患者158例为ACI组,以神经功能缺陷评估量表(national institute of health stroke scale,NIHSS)得分作为依据,又将ACI组分为轻度组(60例)、中度组(52例)以及重度组(46例),同期选择本院正常体检健康者150例作为对照组。采用酶联免疫吸附法(enzyme-linked immunosorbent assay,ELISA)比较血清GFAP、S100B的表达水平;应用受试者工作特征曲线(receiver operating characteristic curve,ROC曲线)评价血清GFAP和S100B在ACI诊断中的有效性。四格表法评价CTA、GFAP和S100B在ACI中的联合应用。结果ACI组血清GFAP和S100B明显高于对照组(P<0.05);在重度组中,GFAP和S100B的表达水平较低,中度组明显增高(P<0.05)。关联研究发现,ACI组患者的血清GFAP与S100B之间存在着显著的相关性(P<0.05)。ROC结果显示,血清GFAP和S100B结合对曲线下面积(area under curve,AUC)的检测优于单独检测(P<0.05)。经四格表分析,CTA与GFAP、S100B联合诊断ACI的敏感性和特异度分别为91.8%和84.0%。三者联合诊断特异度高于CTA、GFAP、S100B单独诊断的特异度(P<0.05)。结论ACI患者血清中GFAP、S100B表达水平升高,CTA联合GFAP、S100B可明显提高ACI诊断的价值。
