Modulation of MMP-2 and TIMP-2 by low dose radiation in mice bearing S180 sarcoma

Objective To investigate the inhibition of low dose radiation(LDR) on S180 sarcomas and its modulation of MMP-2 and TIMP-2 in mice.Methods S180 subcutaneously implanted tumor model mice were randomly divided into two groups: control(N) and low dose radiation(LDR) groups. N mice were sacrificed after 12 h, whereas LDR mice were sacrificed after 12(LDR-12 h), 24(LDR-24 h), 48(LDR-48 h), and 72(LDR-72 h) h. Thereafter, we measured the tumor volumes. Histopathology was performed, and P-V immunohistochemistry was applied to assess MMP-2 and TIMP-2 expression.Results Compared with the control group, the tumor growth was significantly inhibited in the LDR groups(P < 0.05). MMP-2 expression was considerably reduced in LDR-24h(P < 0.05) and LDR-48h(P < 0.05), whereas the change of TIMP-2 was not obvious in the LDR groups(P > 0.05) in contrast to that of the control group.Conclusion LDR can effectively suppress the growth of S180 implanted tumors by reducing MMP-2, which is associated with invasion and metastasis.

西蒙1号甘薯茎叶抗肿瘤的实验研究

目的从西蒙１号甘薯茎叶中提取不同部位，并筛选其抗肿瘤有效部位。方法用水及有机溶剂回流法，从西蒙１号甘薯茎叶中获得其水提取物（ＳＷ）、乙醇提取物（ＳＡ）、氯仿提取物（ＳＣ），以Ｋ５６２、ＥＡＣ、Ｈ２２等癌细胞株及Ｓ１８０、Ｕ１４荷瘤鼠进行体内外抗肿瘤活性筛选。结果ＳＷ、ＳＣ对Ｋ５６２、ＥＡＣ、Ｈ２２的ＩＣ５０为１７６．７～１６１６．６μｇ／ｍｌ，ＳＡ对Ｋ５６２、ＥＡＣ、Ｈ２２的ＩＣ５０分别为２３．８，６２．９，１０６．３μｇ／ｍｌ。ＳＡ２．４ｇ／ｋｇ口服１０ｄ，对Ｓ１８０、Ｕ１４荷瘤鼠的抑瘤率分别为４８．２％，５３．４％。结论西蒙１号甘薯茎叶有一定的体内外抗肿瘤活性，其乙醇提取物为抗肿瘤活性部位。

中肺合剂对S180肉瘤、Lewis肺癌荷瘤小鼠抑瘤作用实验研究

[目的]探讨中肺合剂对S180肉瘤、Lewis肺癌荷瘤小鼠的抑瘤作用,并初步探索其机制。[方法]采用S180肉瘤、Lewis肺癌移植瘤小鼠模型,分荷瘤阴性对照组、低、中、高剂量中肺合剂组和CTX阳性对照组,观察中肺合剂对两种荷瘤小鼠肿瘤生长的抑制作用;运用EnVision免疫组化染色法观察中肺合剂对Lewis肺癌荷瘤小鼠肿瘤组织cyclinD1周期蛋白表达的影响。[结果]中肺合剂低、中、高剂量组和CTX组对移植性荷S180小鼠的抑瘤率分别为16.48%、29.73%、23.51%、41.87%;与阴性对照组相比,中肺合剂低、中、高剂量组和CTX组瘤重均有显著性差异(P<0.05)。中肺合剂低、中、高剂量组和CTX组对移植性荷Lewis肺癌小鼠的抑制率分别为22.33%、39.02%、25.48%、64.16%;与阴性对照组相比,低剂量组和高剂量组瘤重均有显著性差异(P<0.05),中剂量组和CTX化疗组瘤重均有非常显著性差异(P<0.01)。中肺合剂能下调Lewis肺癌小鼠肿瘤组织cyclinD1蛋白的表达水平,中、高剂量组阳性表达率与阴性对照组相比,有显著性差异(P<0.05)。[结论]中肺合剂对S180和Lewis荷瘤小鼠有明显的抑瘤作用,量-效关系呈抛物线型,其抑瘤作用可能与下调肿瘤组织cyclinD1蛋白表达水平有关。