目的研究SIRT3在不同热量饮食下大鼠肾脏的表达变化规律。方法取12月龄自由饮食、热量限制、高热量饮食、健康雄性Fisher344大鼠肾组织进行衰老相关β-半乳糖苷酶(SA-β-gal)活性染色,Western印迹法(Western blot assay)和免疫组织化学...目的研究SIRT3在不同热量饮食下大鼠肾脏的表达变化规律。方法取12月龄自由饮食、热量限制、高热量饮食、健康雄性Fisher344大鼠肾组织进行衰老相关β-半乳糖苷酶(SA-β-gal)活性染色,Western印迹法(Western blot assay)和免疫组织化学法检测肾脏组织中SIRT3的表达变化与定位,并用Western blot检测p16INK4A在肾脏组织中的表达变化。结果大鼠肾脏组织SA-β-gal活性随饮食中热量的增长逐渐增强(P<0.05);p16INK4A表达随饮食中热量增加逐渐增强(P<0.05)。Western blot亦显示SIRT3蛋白表达随饮食中热量的增加逐渐减弱(P<0.05)。免疫组化结果显示,SIRT3蛋白主要表达于大鼠肾小管中,其在肾小球也有微量表达,且跟Western blot结果一致,随饮食中热量增长表达增加(P<0.05)。结论热量限制能延缓大鼠肾脏衰老,SIRT3可能与高热量引起的肾脏衰老有密切关系。展开更多
This study was designed to investigate the effect of 3 D TECA hydrogel on the inflammatory-induced senescence marker, and to assess the influence of the gel on the periodontal lig-ament fibroblasts(PDLFs) migration in...This study was designed to investigate the effect of 3 D TECA hydrogel on the inflammatory-induced senescence marker, and to assess the influence of the gel on the periodontal lig-ament fibroblasts(PDLFs) migration in wound healing in vitro. PDLFs were cultured with20 ng/ml TNF-α to induce inflammation in the presence and absence of 50 μM 3 D TECA gelfor 14 d. The gel effect on the senescence maker secretory associated-β-galactosidase(SA-β-gal) activity was measured by a histochemical staining. Chromatin condensation and DNAsynthesis of the cells were assessed by 4,6-diamidino-2-phenylindole and 5-ethynyl-2-deoxyuridine fluorescent staining respectively. For evaluating fibroblasts migration, scratchwound healing assay and Pro-Plus Imaging software were used. The activity of senescencemarker, SA-β-gal, was positive in the samples with TNF-α-induced inflammation. SA-β-galpercentage is suppressed( > 65%, P < 0.05) in the treated cells with TECA gel as compared tothe non-treated cells. Chromatin foci were obvious in the non-treated samples. DNA syn-thesis was markedly recognized by the fluorescent staining in the treated compared to non-treated cultures. Scratch wound test indicated that the cells migration rate was significantlyhigher(14.9 μm^2/h, P < 0.05) in the treated versus(11 μm^2/h) for control PDLFs. The new for-mula of 3 D TECA suppresses the inflammatory-mediated cellular senescence and enhancedfibroblasts proliferation and migration. Therefore, 3 D TECA may be used as an adjunct to ac-celerate repair and healing of periodontal tissues.展开更多
Aging is a natural physiological process with various challenges, related to the loss of homeostasis within the organism, which is not a disease, but a significantly strong risk factor for multiple diseases, including...Aging is a natural physiological process with various challenges, related to the loss of homeostasis within the organism, which is not a disease, but a significantly strong risk factor for multiple diseases, including myocardial infarction, stroke, some age-related cancers, macular degeneration, osteoarthritis, neurodegeneration, and many others. In the body, the main manifestation of aging is cellular aging, which exists within tissues and has a local or global impact on tissue function. However, the lack of effective aging detection tools has always been an issue that cannot be ignored in the field of aging research. Therefore,it is necessary to construct a non-invasive tool for in vivo detection of aging. Here, we show that the photoacoustic probe(LGAL), which has peak excitation and emission wavelengths in the near-infrared optical window, binds in vivo and at high contrast to the hallmark of aging, and allows for the microscopic imaging of aging through the intact mice. Firstly, this tool LGAL has been successfully applied to detect senescence in cells, displaying stronger photoacoustic signals than normal cells. Then, by using the photoacoustic probe, the blood vessels and tissues inside the mice can be visualized. Young and elderly mice exhibit varying intensities of photoacoustic signals, marking the first time a probe has been used to explore the aging of blood vessels and tissues inside the mice. Finally, we monitored the changes in the degree of aging during tumor treatment under photoacoustic(PA) imaging for the first time. As the treatment time increased, the degree of aging of the tumor gradually deepened. We expect the powerful tool could be a noninvasive and powerful tool for the study of aging biology.展开更多
基金support from the Ministry of Science, Technology and Innovation for provid-ing the grant number (MOSTI SF01.01.09)the Ministryof Higher Education for providing the Research AcculturationGrant Scheme (RAGS 119/2013)UiTM DANA (RIF 751/2012)
文摘This study was designed to investigate the effect of 3 D TECA hydrogel on the inflammatory-induced senescence marker, and to assess the influence of the gel on the periodontal lig-ament fibroblasts(PDLFs) migration in wound healing in vitro. PDLFs were cultured with20 ng/ml TNF-α to induce inflammation in the presence and absence of 50 μM 3 D TECA gelfor 14 d. The gel effect on the senescence maker secretory associated-β-galactosidase(SA-β-gal) activity was measured by a histochemical staining. Chromatin condensation and DNAsynthesis of the cells were assessed by 4,6-diamidino-2-phenylindole and 5-ethynyl-2-deoxyuridine fluorescent staining respectively. For evaluating fibroblasts migration, scratchwound healing assay and Pro-Plus Imaging software were used. The activity of senescencemarker, SA-β-gal, was positive in the samples with TNF-α-induced inflammation. SA-β-galpercentage is suppressed( > 65%, P < 0.05) in the treated cells with TECA gel as compared tothe non-treated cells. Chromatin foci were obvious in the non-treated samples. DNA syn-thesis was markedly recognized by the fluorescent staining in the treated compared to non-treated cultures. Scratch wound test indicated that the cells migration rate was significantlyhigher(14.9 μm^2/h, P < 0.05) in the treated versus(11 μm^2/h) for control PDLFs. The new for-mula of 3 D TECA suppresses the inflammatory-mediated cellular senescence and enhancedfibroblasts proliferation and migration. Therefore, 3 D TECA may be used as an adjunct to ac-celerate repair and healing of periodontal tissues.
基金financially supported by National Natural Science Foundation of China (Nos.21877048, 22077048, and 22277014)Guangxi Natural Science Foundation (Nos.2021GXNSFDA075003,AD21220061)the startup fund of Guangxi University (No.A3040051003)。
文摘Aging is a natural physiological process with various challenges, related to the loss of homeostasis within the organism, which is not a disease, but a significantly strong risk factor for multiple diseases, including myocardial infarction, stroke, some age-related cancers, macular degeneration, osteoarthritis, neurodegeneration, and many others. In the body, the main manifestation of aging is cellular aging, which exists within tissues and has a local or global impact on tissue function. However, the lack of effective aging detection tools has always been an issue that cannot be ignored in the field of aging research. Therefore,it is necessary to construct a non-invasive tool for in vivo detection of aging. Here, we show that the photoacoustic probe(LGAL), which has peak excitation and emission wavelengths in the near-infrared optical window, binds in vivo and at high contrast to the hallmark of aging, and allows for the microscopic imaging of aging through the intact mice. Firstly, this tool LGAL has been successfully applied to detect senescence in cells, displaying stronger photoacoustic signals than normal cells. Then, by using the photoacoustic probe, the blood vessels and tissues inside the mice can be visualized. Young and elderly mice exhibit varying intensities of photoacoustic signals, marking the first time a probe has been used to explore the aging of blood vessels and tissues inside the mice. Finally, we monitored the changes in the degree of aging during tumor treatment under photoacoustic(PA) imaging for the first time. As the treatment time increased, the degree of aging of the tumor gradually deepened. We expect the powerful tool could be a noninvasive and powerful tool for the study of aging biology.
