It is necessary to establish a rapid and accurate diagnostic method for controlling the epidemic of severe acute respiratory syndrome (SARS). Cooperating with Henan Bioengineering Research Center, we developed a new d...It is necessary to establish a rapid and accurate diagnostic method for controlling the epidemic of severe acute respiratory syndrome (SARS). Cooperating with Henan Bioengineering Research Center, we developed a new diagnostic kit for antibodies to SARS virus based on antigen-capturing ELISA. Here we report the clinical detection results obtained from several hospitals which used this diagnostic kit for SARS diagnosing. Comparing with other detection kits now used, the antigen-capturing ELISA has stronger specificity and higher sensitivity, and more fits for rapid confirming and diagnosing of SARS cases, SARS epidemiological investigation and SARS epidemic source research.展开更多
文摘It is necessary to establish a rapid and accurate diagnostic method for controlling the epidemic of severe acute respiratory syndrome (SARS). Cooperating with Henan Bioengineering Research Center, we developed a new diagnostic kit for antibodies to SARS virus based on antigen-capturing ELISA. Here we report the clinical detection results obtained from several hospitals which used this diagnostic kit for SARS diagnosing. Comparing with other detection kits now used, the antigen-capturing ELISA has stronger specificity and higher sensitivity, and more fits for rapid confirming and diagnosing of SARS cases, SARS epidemiological investigation and SARS epidemic source research.
文摘目的:对国内 SARS 抗体(IgG 和/或总抗体)检测试剂盒的质量进行比较和评价。方法:本文选用4种国内 SARS 抗体ELISA 检测试剂盒分别检测已知同样样品,并对结果进行比较。结果:4种试剂盒的阳性参考品符合率均为100%(18/18),阴性参考品符合率均为100%(20/20);3批 D 试剂盒的精密性(10/10)试验的 CV 值均在3%以下,A 试剂盒及3批 B 试剂盒的的 CV 值均在5%～10%之间,3批 C 试剂盒的 CV 值均在10%～15%之间;B、C、D3种试剂盒的灵敏度均达到5/7或以上,其中第1批 C 试剂盒及前2批 D 试剂盒灵敏度较高,均达到了7/7。结论:4种试剂盒均有较好的灵敏度、特异性及检出率。
