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Control of highly pathogenic avian influenza through vaccination 被引量:1
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作者 Xianying Zeng Jianzhong Shi Hualan Chen 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2024年第5期1447-1453,共7页
The stamping-out strategy has been used to control highly pathogenic avian influenza viruses in many countries,driven by the belief that vaccination would not be successful against such viruses and fears that avian in... The stamping-out strategy has been used to control highly pathogenic avian influenza viruses in many countries,driven by the belief that vaccination would not be successful against such viruses and fears that avian influenza virus in vaccinated birds would evolve more rapidly and pose a greater risk to humans.In this review,we summarize the successes in controlling highly pathogenic avian influenza in China and make suggestions regarding the requirements for vaccine selection and effectiveness.In addition,we present evidence that vaccination of poultry not only eliminates human infection with avian influenza virus,but also significantly reduces and abolishes some harmful characteristics of avian influenza virus. 展开更多
关键词 avian influenza CONTROL highly pathogenic VACCINATION
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Genetic and biological properties of H9N2 avian influenza viruses isolated in central China from2020 to 2022
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作者 Libin Liang Yaning Bai +14 位作者 Wenyan Huang Pengfei Ren Xing Li Dou Wang Yuhan Yang Zhen Gao Jiao Tang Xingchen Wu Shimin Gao Yanna Guo Mingming Hu Zhiwei Wang Zhongbing Wang Haili Ma Junping Li 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2024年第8期2778-2791,共14页
The H9N2 subtype of avian influenza virus(AIV)is widely prevalent in poultry and wild birds globally,and has become the predominant subtype circulating in poultry in China.The H9N2 AIV can directly or indirectly(by se... The H9N2 subtype of avian influenza virus(AIV)is widely prevalent in poultry and wild birds globally,and has become the predominant subtype circulating in poultry in China.The H9N2 AIV can directly or indirectly(by serving as a"donor virus")infect humans,posing a significant threat to public health.Currently,there is a lack of in-depth research on the prevalence of H9N2 viruses in Shanxi Province,central China.In this study,we isolated 14 H9N2 AIVs from October 2020 to April 2022 in Shanxi Province,and genetic analysis revealed that these viruses belonged to 7 different genotypes.Our study on animals revealed that the H9N2 strains we identified displayed high transmission efficiency among chicken populations,and exhibited diverse replication abilities within these birds.These viruses could replicate efficiently in the lungs of mice,with one strain also demonstrating the capacity to reproduce in organs like the brain and kidneys.At the cellular level,the replication ability of different H9N2 strains was evaluated using plaque formation assays and multi-step growth curve assays,revealing significant differences in the replication and proliferation efficiency of the various H9N2 viruses at the cellular level.The antigenicity analysis suggested that these isolates could be classified into 2 separate antigenic clusters.Our research provides crucial data to help understand the prevalence and biological characteristics of H9N2 AIVs in central China.It also highlights the necessity of enhancing the surveillance of H9N2 AIVs. 展开更多
关键词 avian influenza virus H9N2 central China PATHOGENICITY ANTIGENICITY
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Antibodies elicited by Newcastle disease virus-vectored H7N9 avian influenza vaccine are functional in activating the complement system
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作者 Zenglei Hu Ya Huang +3 位作者 Jiao Hu Xiaoquan Wang Shunlin Hu Xiufan Liu 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2024年第6期2052-2064,共13页
H7N9 subtype avian influenza virus poses a great challenge for poultry industry.Newcastle disease virus(NDV)-vectored H7N9 avian influenza vaccines(NDV_(vec)H7N9)are effective in disease control because they are prote... H7N9 subtype avian influenza virus poses a great challenge for poultry industry.Newcastle disease virus(NDV)-vectored H7N9 avian influenza vaccines(NDV_(vec)H7N9)are effective in disease control because they are protective and allow mass administration.Of note,these vaccines elicit undetectable H7N9-specific hemagglutination-inhibition(HI)but high IgG antibodies in chickens.However,the molecular basis and protective mechanism underlying this particular antibody immunity remain unclear.Herein,immunization with an NDV_(vec)H7N9 induced low anti-H7N9 HI and virus neutralization titers but high levels of hemagglutinin(HA)-binding IgG antibodies in chickens.Three residues(S150,G151 and S152)in HA of H7N9 virus were identified as the dominant epitopes recognized by the NDV_(vec)H7N9 immune serum.Passively transferred NDV_(vec)H7N9 immune serum conferred complete protection against H7N9 virus infection in chickens.The NDV_(vec)H7N9 immune serum can mediate a potent lysis of HA-expressing and H7N9 virus-infected cells and significantly suppress H7N9 virus infectivity.These activities of the serum were significantly impaired after heat-inactivation or treatment with complement inhibitor,suggesting the engagement of the complement system.Moreover,mutations in the 150-SGS-152 sites in HA resulted in significant reductions in cell lysis and virus neutralization mediated by the NDV_(vec)H7N9 immune serum,indicating the requirement of antibody-antigen binding for complement activity.Therefore,antibodies induced by the NDV_(vec)H7N9 can activate antibody-dependent complement-mediated lysis of H7N9 virus-infected cells and complement-mediated neutralization of H7N9 virus.Our findings unveiled a novel role of the complement in protection conferred by the NDV_(vec)H7N9,highlighting a potential benefit of engaging the complement system in H7N9 vaccine design. 展开更多
关键词 H7N9 subtype avian influenza virus NDV vector vaccine antibody immunity COMPLEMENT protection
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H7N9 avian influenza with first manifestation of occipital neuralgia:A case report
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作者 Jie Zhang 《World Journal of Clinical Cases》 SCIE 2023年第2期434-440,共7页
BACKGROUND Most of the first symptoms of avian influenza are respiratory symptoms,and cases with occipital neuralgia as the first manifestation are rarely reported.CASE SUMMARY A middle-aged patient complaining of par... BACKGROUND Most of the first symptoms of avian influenza are respiratory symptoms,and cases with occipital neuralgia as the first manifestation are rarely reported.CASE SUMMARY A middle-aged patient complaining of paroxysmal pain behind the ear was admitted to our hospital.The patient’s condition changed rapidly,and high fever,unexpected respiratory failure,and multiple organ failure developed rapidly.The patient was diagnosed with H7N9 avian influenza based on etiology.CONCLUSION We believe that the etiology of occipital neuralgia is complex and could be the earliest manifestation of severe diseases.The possibility of an infectious disease should be considered when occipital neuralgia is accompanied by fever.Avian influenza is one of these causative agents. 展开更多
关键词 Occipital neuralgia avian influenza RESPIRATORY INFECTIOUS Case report
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Development and Characterization of Monoclonal Antibody Specific to Nuclear Protein of Avian Influenza Virus Type A 被引量:7
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作者 李娜 秦爱建 +2 位作者 邵红霞 金文杰 刘岳龙 《Agricultural Science & Technology》 CAS 2008年第1期60-63,66,共5页
Five monoclonal antibodies(Mabs) to nuclear protein of avain influenza virus(AIV) were developed by syncretizing SP 2/0 and the spleen cells from BALB of mice immuized with H9 subtype AIV. Specificity of these Mab... Five monoclonal antibodies(Mabs) to nuclear protein of avain influenza virus(AIV) were developed by syncretizing SP 2/0 and the spleen cells from BALB of mice immuized with H9 subtype AIV. Specificity of these Mabs were identified by immunofluorescent assay(IFA) and enzyme linked immunosorbent assay (ELISA). These five Mabs which were named as AIV-NP-2C3, AIV-NP-6A5, AIV-NP-3 H9, AIV-NP-7B4, AIV-NP-2H4 could react with all viruses of AIV-H9 strains in tests. The result of Western blotting showed that only the 60 ku protein antigen of AIV-H9 could be recognized by the Mabs but never recognized by New castle disease virus, REV and infectious bursa disease virus. The result of preliminary application showed that avian influenza viruses could be deetected bv Mabs in IFA and ELISA. All these Mabs will probably play important roles in preventing and monitoring avian influenza viruses. 展开更多
关键词 avian influenza virus NP Monoclonal antibody Immunofluorescent assay (IFA) ELISA
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Designing Primers for H5 and H7 Subtypes of Avian Influenza Virus and Multiplex RT-PCR Amplification 被引量:5
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作者 张文慧 郭华 +2 位作者 王伟利 刘明 钱爱东 《Agricultural Science & Technology》 CAS 2008年第1期15-17,共3页
[Objective] The research aimed to design primers that are suitable for detecting H5 and H7 subtypes of avian influenza virus (AIV) ; [Method] DNAStar was used to analyze the homology of the sequences of H5 and H7 su... [Objective] The research aimed to design primers that are suitable for detecting H5 and H7 subtypes of avian influenza virus (AIV) ; [Method] DNAStar was used to analyze the homology of the sequences of H5 and H7 subtypes of AIV accessed in GenBank, and design primers( by Primer Premier 5.0) on high homologous region of these sequences, and then amplified by RT-PCR. [Result] The multiplex RT-PCR amplification, agarose gel electrophoresis and sequencing results showed that the self-designed primers are successful for detecting AIV. [Conclusion] It is feasible to rapidly diagnose AIV through this method. 展开更多
关键词 avian influenza virus Primer Premier 5.0 DNAStar Multiplex RT-PCR amplification
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Sequence Analysis of HA Genes from Three H9N2 Subtype Avian Influenza Viruses 被引量:2
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作者 韩春华 林健 +3 位作者 刘月焕 潘洁 马明 刘永宏 《Animal Husbandry and Feed Science》 CAS 2009年第1期32-35,共4页
[ Objective] The study aimed to understand the genetic characters of H9N2 subtype avian influenza viruses isolated in Belling area. [ Method] HA genes of three H9N2 subtype avian influenza viruses A/Chicken/Beijing/xu... [ Objective] The study aimed to understand the genetic characters of H9N2 subtype avian influenza viruses isolated in Belling area. [ Method] HA genes of three H9N2 subtype avian influenza viruses A/Chicken/Beijing/xu/00, A/Chicken/Beijing/bei/00 and A/Chicken/Beijing/ liu/00 were amplified by RT-PCR and then sequenced. [ Result] The results of phylogenetic analysis showed that A/Chicken/Beijing/xu/00, A/ Chicken/Beijing/bei/00 and A/Chicken/Beijing/liu/00 shared the nucleotide homologies of 84.8% ( Dk/HK/Y439/97 ) -98.0% ( Ck/GX17/00 ), 85.1% (Dk/HK/Y439/97) - 99.1% ( Ck/GXl 7/00), 90.7% ( Ck/BJ/3/01 ) - 99.1% (Ck/GX17/00) with the isolates from Hongkong and other are- as of Chinese Mainland respectively. At the same time, the analysis of amino acid indicated that the three isolates belonged to low pathogenic H9N2 isolates of avian origin. The 226^th amino acid of them were L ( Leu), suggesting their high binding affinity to human cells. There were seven glyco- sylation sites in HA protein, five from HA1 and two from HA2. [ Cenclusien] By analysis at molecular level, it could be concluded that A/Chicken/ Beijing/xu/00, A/Chicken/Beijing/bei/00 and A/Chicken/Beijing/liu/00 were low pathogenic H9N2 isolates of avian origin. 展开更多
关键词 H9N2 subtype avian influenza virus HA gene Sequence analysis
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Complete Genome Sequencing and Genetic Variation Analysis of Two H9N2 Subtype Avian Influenza Virus Strains 被引量:2
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作者 沈佳 章振华 +3 位作者 姜北宇 李林 景小冬 张建伟 《Agricultural Science & Technology》 CAS 2011年第2期291-294,共4页
[Objective] The study aimed to investigate the genetic variation characters of entire sequences between two H9N2 subtype avian influenza virus strains and other reference strains.[Method] The entire sequences of 8 gen... [Objective] The study aimed to investigate the genetic variation characters of entire sequences between two H9N2 subtype avian influenza virus strains and other reference strains.[Method] The entire sequences of 8 genes were obtained by using RT-PCR,and these sequences were analyzed with that of six H9N2 subtype avian influenza isolates in homology comparison and genetic evolution relation.[Result] The results showed that the nucleotide sequence of entire gene of the strain shared 91.1%-95.4% homology with other seven reference strains,and PG08 shared the highest homology 91.3% with C/BJ/1/94;ZD06 shared the highest homology 92.3% with D/HK/Y280/97.HA cleavage sites of two H9N2 subtype avian influenza virus isolated strains were PARSSR/GLF,typical of mildly pathogenic avian influenza virus.[Conclusion] Phylogenetic tree for entire gene of eight strains showed that the genetic relationship was the closest between ZD06 and C/Pak/2/99 strains,which belonged to the Eurasian lineage;PG08 shared the highest homology 91.3% with ZD06,it may be the product of gene rearrangements of other sub-lines. 展开更多
关键词 avian influenza virus H9N2 subtype Complete genome Sequence analysis
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候鸟迁徙与禽流感(Avian Influenza,AI)传播 被引量:13
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作者 张晓红 张虎芳 《太原师范学院学报(自然科学版)》 2005年第4期81-84,共4页
2003年以来,亚洲许多国家接连爆发了高致病性禽流感,对家禽养殖业已造成了巨大损失.通过分析禽流感和禽流感疫情暴发的季节性和区域性特点与鸟类迁徙特点的关系.我们认为鸟类迁徙在禽流感的传播中起重要作用.
关键词 候鸟 迁徒 禽流感
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Sequence Comparison and Analysis of HA Gene of Four H9N2 Avian Influenza Virus Isolates
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作者 章振华 于博 +4 位作者 姜北宇 钱爱东 李林 景小冬 张建伟 《Agricultural Science & Technology》 CAS 2009年第5期55-58,64,共5页
[ Objective] To determine the HA gene sequences of four H9N2 Avian influenza virus (AIV) strains and carry out comparative analysis so as to understand the difference and variation pattern of each strain from the an... [ Objective] To determine the HA gene sequences of four H9N2 Avian influenza virus (AIV) strains and carry out comparative analysis so as to understand the difference and variation pattern of each strain from the angle of molecular biology and to know the distribution and epidemic law of H9N2 AIV. [Method] One pair of primers was designed referring to HA gene sequences of H9N2 AIV. The HA genes of A/Chicken/Hebei/WD/98 (H9N2; WD98 for short), A/Chicken/Hebei/ZD/04 (H9N2; ZD04 for short)), A/Chicken/Beijing/MY/06 (H9N2; MY06 for short) ), and A/Chicken/Beijing/PG/08 (H9N2; PG08 for short)) were amplified, cloned and sequenced. Then the HA gene sequences of these strains were compared with that of 10 H9N2 AIV stains in GenBank. [Result] The ORF of HA genes of the four strains was 1 683 bp in size, encoding 516 amino acids. The HA gene sequences of the four strains, WD98, MY06, PG08, and ZD04, were 82.6% -95.1%, 83.0% -99.0%, 82.7% -95.5%, and 81.3% -95.7% homologous to that of the 10 H9N2 AIV stains, respectively. And the homology of amino acid was respectively 86.6% -96.3%, 86.6% -97.9%, 87.0% -97.1%, and 86.9% -97.3%. [ Conclusion] The HA gene has greatly high homology among different strains. 展开更多
关键词 avian influenza H9N2 Sequence analysis Hemagglutinin gene
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不变与变:从“SARS”到“Swine Influenza”的中文译名谈起
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作者 何学兵 《兰州教育学院学报》 2018年第1期153-155,共3页
SARS与Swine Influenza为发生在本世纪初的两场重大传染疾病,其中SARS的中文译名一直未变,译为"非典";Swine Influenza则从开始的"猪流感"更名为"甲型H1N1流感"。本文尝试从翻译与传播的视角探讨这两种... SARS与Swine Influenza为发生在本世纪初的两场重大传染疾病,其中SARS的中文译名一直未变,译为"非典";Swine Influenza则从开始的"猪流感"更名为"甲型H1N1流感"。本文尝试从翻译与传播的视角探讨这两种疾病中文译名的不变与变,分析背后的原因,并提出SARS中文译名的改进译法。 展开更多
关键词 sars SWINE influenza 翻译 传播 改译
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The role of wild goose(Anser) populations of Russia and theTibet Plateau in the spread of the avian influenza virus 被引量:2
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作者 Mariya V.SIVAY Nikita Y.SILKO +5 位作者 Kirill A.SHARSHOV Aleksander V.PROKUDIN 李来兴 杨敏 操胜 Aleksander M.SHESTOPALOV 《Chinese Birds》 2011年第3期140-146,共7页
Wild birds of the orders Anseriformes and Charadriiformes represent a natural reservoir of low pathogenic avian influenza(LPAI) viruses(family Orthomyxoviridae).Wild geese(order Anseriformes)relating to waterfowls und... Wild birds of the orders Anseriformes and Charadriiformes represent a natural reservoir of low pathogenic avian influenza(LPAI) viruses(family Orthomyxoviridae).Wild geese(order Anseriformes)relating to waterfowls undertake extensive migration flights reaching thousands of kilometers.Isolation of the avian influenza virus(AIV) from wild geese is quite low or absent.The aims of this study are to monitor the AIV in different wild goose species,nesting on Russian territory and the Tibet Plateau and to analyze the derived data for the purpose of determining the role of these wild bird species in spreading pathogens.In our study 3245 samples from nine wild goose species in nine regions of Russia and on the territory of the Tibet Plateau(the Xizang Autonomous Region) were tested and no AIV were detected.Our study shows the non-essential role of wild geese in the spread of the AIV over long distances and reaches theconclusion that geese are probably not natural reservoirs for the primary viruses.However,further inquiry of AIV in wild goose populations is required.Studies of wild geese and AIV ecology will allow us to obtainmore information about pathogen-host relationships and to make arrangements for the maintenance ofwild goose populations. 展开更多
关键词 avian influenza virus wild geese RUSSIA Tibet Plateau
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Study on Piezoelectric Immunosensor for the Detection of H9-subtype Avian Influenza Virus 被引量:2
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作者 詹爱军 胡云发 +3 位作者 王新卫 刘靖清 卞红春 陈枝楠 《Agricultural Science & Technology》 CAS 2011年第10期1517-1520,共4页
[Objective] The aim is to develop the piezoelectric immunosensor to detect H9-subtype avian influenza virus(AIV).[Method] The immunosensor chip was constructed by self-assembling mercaptopmpionic acid(MPA) to be m... [Objective] The aim is to develop the piezoelectric immunosensor to detect H9-subtype avian influenza virus(AIV).[Method] The immunosensor chip was constructed by self-assembling mercaptopmpionic acid(MPA) to be monolayer on the silver-coated electrode of quartz crystal and coupling the monoclonal antibody to H9 subtype AIV with N-ethy-N'-(3-dimethyl aminopropyl)carbodiimide hydrochloride(EDC) and N-hydroxysuccinimide(NHS).The immunosensor to detect H9 subtype AIV was established.[Result] The results showed that the immunosensor displayed better specificity to H9 AIV and had no response to H5AIV and NDV when it was used for detection.The sensitivity test indicated the detection sensitivity for the H9 subtype AIV could reach 20-100 EID50.[Conclusion] The research provided a foundation for further research on the immunosensor for detecting AIV and it could be a new approach to detect other related viruses. 展开更多
关键词 Piezoelectric immunosensor Biological self-assembly method H9 subtype avian influenza virus
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Astragalus polysaccharide enhances immunity and inhibits H9N2 avian influenza virus in vitro and in vivo 被引量:49
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作者 Sanpha Kallon Xiaorong Li +7 位作者 Jun Ji Cuiying Chen Qianyun Xi Shuang Chang Chunyi Xue Jingyun Ma Qingmei Xie Youngliang Zhang 《Journal of Animal Science and Biotechnology》 SCIE CAS 2013年第4期325-335,共11页
This study investigated the humoral immunization of Astragalus polysaccharide (APS) against HgN2 avian influenza virus (H9N2 AIV) infection in chickens. The effects of APS treatment on H9N2 infection was evaluated... This study investigated the humoral immunization of Astragalus polysaccharide (APS) against HgN2 avian influenza virus (H9N2 AIV) infection in chickens. The effects of APS treatment on H9N2 infection was evaluated by an Mqq- [3(4, 5-dimethylthiazol-2-yl)-2, 3-diphenyl tetrazolium bromide] assay and analysis of MFIC and cytokine mRNA expression. The effect on lymphocyte and serum antibody titers in vivo was also investigated. IL-4, IL-6, IL-10, LITAF, IL-12 and antibody titers to H9N2 AIV wet enhanced in the first week after APS treatment. The results indicated that APS treatment reduces H9N2 AIV replication and promotes early humoral immune responses in young chickens.This study investigated the humoral immunization of Astragalus polysaccharide (APS) against HgN2 avian influenza virus (H9N2 AIV) infection in chickens. The effects of APS treatment on HgN2 infection was evaluated by an M]q- [3(4, 5-dimethylthiazol-2-yl)-2, 3-diphenyl tetrazolium bromide] assay and analysis of MHC and cytokine mRNA expression. The effect on lymphocyte and serum antibody titers in vivo was also investigated. IL-4, IL-6, IL-10, LITAF, IL-12 and antibody titers to PIgN2 AIV were enhanced in the first week after APS treatment. The results indicated that APS treatment reduces HgN2 AIV replication and promotes early humoral immune responses in young chickens. 展开更多
关键词 Astrogalus polysaccharide HgN2 avian influenza virus Immune effect
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Risk assessment on the epidemics of human infection with a novel avian influenza A (H7N9) virus in Jiangsu Province, China 被引量:9
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作者 Wendong Liu Yefei Zhu +7 位作者 Xian Qi Ke Xu Aihua Ge Hong Ji Jing Ai Changjun Bao Fenyang Tang Minghao Zhou 《The Journal of Biomedical Research》 CAS 2013年第3期163-166,共4页
A novel avian influenza A (H7N9) virus was discovered in February 2013 in China and has resulted in more than 100 comfirmed human infections including 26 fatal cases as of May 2, 2013. The situation raises many ur- ... A novel avian influenza A (H7N9) virus was discovered in February 2013 in China and has resulted in more than 100 comfirmed human infections including 26 fatal cases as of May 2, 2013. The situation raises many ur- gent questions and global public health concerns. In this study, epidemiologic characteristics of infected human cases in Jiangsu province were analyzed and risk assessment was undertaken based on the information available. Briefly, it is highly unlikely that a pandemic of human infection with avian influenza A (HTN9) virus will happen in Jiangsu Province in the near future. Iia the end, some measures are recommended to prevent the situation from becoming worse. 展开更多
关键词 avian influenza H7N9 risk assessment
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Development of a reverse-transcription loop-mediated isothermal amplification assay to detect avian influenza viruses in clinical specimens 被引量:3
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作者 SHI Lin YU Xue-wu +7 位作者 YAO Wei YU Ben-liang HE Li-kun GAO Yuan ZHANG Yun-xian TIAN Guo-bin PING Ji-hui WANG Xiu-rong 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2019年第7期1428-1435,共8页
In recent years,the avian influenza has brought not only serious economic loss to the poultry industry in China but also a serious threat to human health because of the avian influenza virus(AIV) gene recombination an... In recent years,the avian influenza has brought not only serious economic loss to the poultry industry in China but also a serious threat to human health because of the avian influenza virus(AIV) gene recombination and reassortment.Until now,traditional RT-PCR,fluorescence RT-PCR and virus isolation identification have been developed and utilized to detect AIV,but these methods require high-level instruments and experimental conditions,not suitable for the rapid detection in field and farms.In order to develop a rapid,sensitive and practical method to detect and identify AIV subtypes,4 specific primers to the conserved region of AIV M gene were designed and a loop-mediated isothermal amplification(RT-LAMP) method was established.Using this method,the M gene of H1–H16 subtypes of AIV were amplified in 30 min with a water bath and all 16 H subtypes of AIV were able to be visually identified in presence of fluorescein,without cross reaction with other susceptible avian viruses.In addition,the detection limit of the common H1,H5,H7,and H9 AIV subtypes with the RT-LAMP method was 0.1 PFU(plaque-forming unit),which was 10 times more sensitive than that using the routine RT-PCR.Further comparative tests found that the positivity rate of RT-LAMP on detecting clinical samples was 4.18%(14/335) comparing with 3.58%(12/335) from real-time RT-PCR.All these results suggested that the RT-LAMP method can specifically detect and identify AIV with high sensitivity and can be considered as a fast,convenient and practical method for the clinic test and epidemiological investigation of AIV. 展开更多
关键词 avian influenza virus(AIV) RT-LAMP diagnostic method clinical specimens
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Subtype Identification of Avian Influenza Virus on DNA Microarray 被引量:5
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作者 WANG Xiu-rong YU Kang-zhen DENG Guo-hua SHI Rui LIU Li-ling QIAO Chuan-ling BAO Hong-mei KONG Xian-gang CHEN Hua-lan 《Agricultural Sciences in China》 CAS CSCD 2005年第9期700-706,共7页
We have developed a rapid microarray-based assay for the reliable detection of H5, H7 and H9 subtypes of avian influenza virus (AIV). The strains used in the experiment were A/Goose/Guangdong/1/96 (H5N1), A/Africa... We have developed a rapid microarray-based assay for the reliable detection of H5, H7 and H9 subtypes of avian influenza virus (AIV). The strains used in the experiment were A/Goose/Guangdong/1/96 (H5N1), A/African starling/983/79 (H7N1) and A/Turkey/Wiscosin/1/66 (H9N2). The capture DNAs clones which encoding approximate 500-bp avian influenza virus gene fragments obtained by RT-PCR, were spotted on a slide-bound microarray. Cy5-labeled fluorescent cDNAs, which generated from virus RNA during reverse transcription were hybridized to these capture DNAs. These capture DNAs contained multiple fragments of the hemagglutinin and matrix protein genes of AIV respectively, for subtyping and typing AIV. The arrays were scanned to determine the probe binding sites. The hybridization pattern agreed approximately with the known grid location of each target. The results show that DNA microarray technology provides a useful diagnostic method for AIV. 展开更多
关键词 avian influenza virus DNA microarray Subtype identification
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Genetic Variation Analysis on the Whole Genomic Sequence of a H9N2 Subtype Avian Influenza Virus Isolate 被引量:7
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作者 YU Bo ZHANG Zhen-hua +4 位作者 JIANG Bei-yu QIAN Ai-dong LI Lin JING Xiao-dong ZHANG Jian-wei 《Animal Husbandry and Feed Science》 CAS 2009年第11期33-36,共4页
A Objective3 This study was to understand the genetic variation characters of the H9N2 subtype avian influenza virus isolate (A/Chicken/ Hebei/WD/98, abbreviated as WD98) by comparing with other reference strains. I... A Objective3 This study was to understand the genetic variation characters of the H9N2 subtype avian influenza virus isolate (A/Chicken/ Hebei/WD/98, abbreviated as WD98) by comparing with other reference strains. I-Method3 Eight complete genes were amplified by RT-PCR and sequenced. The homology and genetic evolution relationship were analyzed between these sequences and that of the seven reference strains. [Result] The whole genomic sequence of WD98 strain was 91.1% -95.8% homologous to that of seven reference strains tested. This isolate shared the highest homology (95.8%) to D/HK/Y280/97 and the lowest homology (91.1% ) to C/Pak/2/99. The HA cleavage site of the WD98 strain was R-S-S-R G, and the 226th amino acid at receptor-binding site was Gin. [ Condmion] WD98 strain belongs to mildly pathogenic avian in- fluenza virus and may not infect human. The genetic relationship is the closest between A/Chicken/Hebei/wD/98 and A/duck/HongKong/Y280/ 97, both of which belong to the sub-line of A/Chicken/Beijing/1/94 in Eurasian line. And A/Chicken/Hebei/WD/98 and A/Chicken/Beijing/1/94 are genetically distant within the same sub-line. 展开更多
关键词 avian influenza virus H9N2 subtype Genomic sequence Genetic variation
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Cross-neutralizing Anti-hemagglutinin Antibodies Isolated from Patients Infected with Avian Influenza A(H5N1) Virus 被引量:3
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作者 SUN Ying CAO Yang +11 位作者 LI Zi BAI Tian ZHANG Hong HU Shi Xiong LI Fang Cai ZHAO Xiang CHEN Yong Kun LU Jian LIU Li Qi WANG Da Yan SHU Yue Long ZHOU Jian Fang 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 2020年第2期103-113,共11页
Objective To recover broad-neutralizing monoclonal antibodies(Bn Abs)from avian influenza A(H5N1)virus infection cases and investigate their genetic and functional features.Methods We screened the Abs repertoires of e... Objective To recover broad-neutralizing monoclonal antibodies(Bn Abs)from avian influenza A(H5N1)virus infection cases and investigate their genetic and functional features.Methods We screened the Abs repertoires of expanded B cells circulating in the peripheral blood of H5N1 patients.The genetic basis,biological functions,and epitopes of the obtained Bn Abs were assessed and modeled.Results Two Bn Abs,2-12 D5,and 3-37 G7.1,were respectively obtained from two human H5N1 cases on days 12 and 21 after disease onset.Both Abs demonstrated cross-neutralizing and Ab-dependent cellular cytotoxicity(ADCC)activity.Albeit derived from distinct Ab lineages,i.e.,V^H1-69-D2-15-JH^4(2-12D5)and V^H1-2-D3-9-JH^5(3-32 G7.1),the Bn Abs were directed toward CR6261-like epitopes in the HA stem,and HA2 I45 in the hydrophobic pocket was the critical residue for their binding.Signature motifs for binding with the HA stem,namely,IFY in VH1-69-encoded Abs and LXYFXW in D3-9-encoded Abs,were also observed in 2-12D5 and 3-32 G7.1,respectively.Conclusions Cross-reactive B cells of different germline origins could be activated and re-circulated by avian influenza virus.The HA stem epitopes targeted by the Bn Abs,and the two Ab-encoding genes usage implied the VH1-69 and D3-9 are the ideal candidates triggered by influenza virus for vaccine development. 展开更多
关键词 V^H1-69 D3-9 avian influenza A(H5N1)virus Cross-neutralizing Antibody
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The Protection Efficacity of DNA Vaccine Encoding Hemagglutinin of H5 Subtype Avian Influenza Virus 被引量:2
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作者 JIANGYong-ping YUKang-zhen DENGGuo-hua TIANGuo-bin QIAOChuan-ling CHENHua-lan 《Agricultural Sciences in China》 CAS CSCD 2004年第12期943-947,共5页
The DNA vaccine pCIHA5 encoding hemagglutinin can protect SPF chicken against lethal H5N1 avian influenza virus challenge. The more characters about its protection efficacity were studied. The protective rates in 10... The DNA vaccine pCIHA5 encoding hemagglutinin can protect SPF chicken against lethal H5N1 avian influenza virus challenge. The more characters about its protection efficacity were studied. The protective rates in 10, 40, 70, 100 and 150 μg groups immunized with pCIHA5 were 12.5 (1/8), 58.3 (7/12), 72.7 (8/11), 50.0 (6/12) and 66.7% (8/12), respectively. The protective rates in 5, 20, 35 and 50 μg groups were 145.5 (5/11), 58.3 (7/12), 58.3 (7/12) and 91.7% (11/12), respectively. The 70, 100 and 5 μg groups have virus shedding of 1/8, 2/6 and 1/5. Though the inactived oil-emulsion vaccine has high HI antibody titers and 100% protective rate, the AGP antibody could be detected after vaccination. Results show that the pCIHA5 is fit to boost by intramuscular injection. This would be useful to the study on gene engineering vaccine of avian influenza virus. 展开更多
关键词 avian influenza virus HEMAGGLUTININ DNA vaccine Protection efficacity
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