To determine whether squamous cell carcin-oma antigen recognized by human T cell 3(SART3)gene can induce antitumor immunity against tumor cells which express the gene,we constructed mouse tumor cells expressing human ...To determine whether squamous cell carcin-oma antigen recognized by human T cell 3(SART3)gene can induce antitumor immunity against tumor cells which express the gene,we constructed mouse tumor cells expressing human SART3(LM8-SART3)and carried out experiments in vitro/vivo.After subcutaneous injection with SART3 gene vaccine,cytotoxic T lymphocyte(CTL)activity in vitro was measured using Cell Counting Kit-8.As for the in vivo part,C3H mice were divided into several groups.One group was challenged with tumor cells after immunity.Another group was treated with the vaccine after tumor implantation.It was found that human SART3 DNA vaccine can elicit a specific CTL reaction from the mouse splenocytes.After vaccination,tumor occurrence and tumor growth speed was reduced.The vaccine also shows activity in tumor treatment.We con-clude that the human SART3 DNA vaccine can induce antitumor ability against tumor cells expressing human SART3(LM8-SART3)in vitro/vivo which may provide new possibilities in antitumor therapy.展开更多
基金This study was supported by the Natural Science Foundation Project of the Health Department of Hubei Province,China.(No.NX200509)。
文摘To determine whether squamous cell carcin-oma antigen recognized by human T cell 3(SART3)gene can induce antitumor immunity against tumor cells which express the gene,we constructed mouse tumor cells expressing human SART3(LM8-SART3)and carried out experiments in vitro/vivo.After subcutaneous injection with SART3 gene vaccine,cytotoxic T lymphocyte(CTL)activity in vitro was measured using Cell Counting Kit-8.As for the in vivo part,C3H mice were divided into several groups.One group was challenged with tumor cells after immunity.Another group was treated with the vaccine after tumor implantation.It was found that human SART3 DNA vaccine can elicit a specific CTL reaction from the mouse splenocytes.After vaccination,tumor occurrence and tumor growth speed was reduced.The vaccine also shows activity in tumor treatment.We con-clude that the human SART3 DNA vaccine can induce antitumor ability against tumor cells expressing human SART3(LM8-SART3)in vitro/vivo which may provide new possibilities in antitumor therapy.
