DNA methylation plays a vital role in the regulation of gene expression in response to environmental stress. However, little is known about the effect of DNA methylation on the cassava polyploidy. In the present study...DNA methylation plays a vital role in the regulation of gene expression in response to environmental stress. However, little is known about the effect of DNA methylation on the cassava polyploidy. In the present study, methylation-sensitive amplified polymorphisms （MSAP） were used to investigate DNA methylation profiles of cassava polyploidy following cold treatment to identify candidate genes involved in response to cold stress. The result showed that the genome-wide DNA methylation polymorphisms accounted for 34.02%-42.56% in SC8 and its autotetraploid exposed to 5 ~C for 2, 8, 24 and 48 h, respectively. The methylation levels of SC8 at 2 h-cold stress were the highest during 48h under cold treatments. With the time extension within 48 h under cold stress, the methylation levels gradually decreased to the same level as the control but DNA methylation levels of cassava autotetraploid were stable within 48 h. For future analysis of the methylation extent, the cold stress induced more DNA methylation than demethylation in SC8, where DNA methylation was consistent with demethylation in its autotetraploid. The expression analysis demonstrated increase in the transcription of one methylated gene and decrease in the transcription of two demethylated genes. The results revealed that gene methylations in specific sites would be a rapidly epigenetic response to cold stress, further elucidating the methylation functions in its autotetraploid.展开更多
1概况ISO/TC10于1947年建立,工作对象主要是机械制图、建筑制图、船舶制图、土木制图及电气制图等各类制图标准化技术委员会其中SC8分技术委员会创立于1982年。TC10/SC8分委员会秘书处设在瑞典,我国是积极成员P,主要的领域是建筑制图的...1概况ISO/TC10于1947年建立,工作对象主要是机械制图、建筑制图、船舶制图、土木制图及电气制图等各类制图标准化技术委员会其中SC8分技术委员会创立于1982年。TC10/SC8分委员会秘书处设在瑞典,我国是积极成员P,主要的领域是建筑制图的符号、简化画法、标识、表示方法、计算机CAD中的图层、设备和建筑管理元数据文件等方面的内容。目前ISO/TC10/SC8有一个工作组WG16 :New forms of constructiondocumentation (新形式建筑文件)。展开更多
2018年11月5-9日,ISO/TC22/SC32/WG8功能安全工作组会议在意大利比萨召开。会议围绕自动驾驶汽车预期功能安全的国际标准ISO21448 Road Vehicles-Safety of the Intended Functionality(SOTIF)开展探讨制定工作。该标准受到全球瞩目,来...2018年11月5-9日,ISO/TC22/SC32/WG8功能安全工作组会议在意大利比萨召开。会议围绕自动驾驶汽车预期功能安全的国际标准ISO21448 Road Vehicles-Safety of the Intended Functionality(SOTIF)开展探讨制定工作。该标准受到全球瞩目,来自中国、法国、德国、美国、英国、以色列、比利时、意大利、瑞典、日本、荷兰、韩国、芬兰、卢森堡、匈牙利、瑞士、爱尔兰等17个国家72名代表参会。展开更多
文摘DNA methylation plays a vital role in the regulation of gene expression in response to environmental stress. However, little is known about the effect of DNA methylation on the cassava polyploidy. In the present study, methylation-sensitive amplified polymorphisms （MSAP） were used to investigate DNA methylation profiles of cassava polyploidy following cold treatment to identify candidate genes involved in response to cold stress. The result showed that the genome-wide DNA methylation polymorphisms accounted for 34.02%-42.56% in SC8 and its autotetraploid exposed to 5 ~C for 2, 8, 24 and 48 h, respectively. The methylation levels of SC8 at 2 h-cold stress were the highest during 48h under cold treatments. With the time extension within 48 h under cold stress, the methylation levels gradually decreased to the same level as the control but DNA methylation levels of cassava autotetraploid were stable within 48 h. For future analysis of the methylation extent, the cold stress induced more DNA methylation than demethylation in SC8, where DNA methylation was consistent with demethylation in its autotetraploid. The expression analysis demonstrated increase in the transcription of one methylated gene and decrease in the transcription of two demethylated genes. The results revealed that gene methylations in specific sites would be a rapidly epigenetic response to cold stress, further elucidating the methylation functions in its autotetraploid.
文摘1概况ISO/TC10于1947年建立,工作对象主要是机械制图、建筑制图、船舶制图、土木制图及电气制图等各类制图标准化技术委员会其中SC8分技术委员会创立于1982年。TC10/SC8分委员会秘书处设在瑞典,我国是积极成员P,主要的领域是建筑制图的符号、简化画法、标识、表示方法、计算机CAD中的图层、设备和建筑管理元数据文件等方面的内容。目前ISO/TC10/SC8有一个工作组WG16 :New forms of constructiondocumentation (新形式建筑文件)。
文摘2018年11月5-9日,ISO/TC22/SC32/WG8功能安全工作组会议在意大利比萨召开。会议围绕自动驾驶汽车预期功能安全的国际标准ISO21448 Road Vehicles-Safety of the Intended Functionality(SOTIF)开展探讨制定工作。该标准受到全球瞩目,来自中国、法国、德国、美国、英国、以色列、比利时、意大利、瑞典、日本、荷兰、韩国、芬兰、卢森堡、匈牙利、瑞士、爱尔兰等17个国家72名代表参会。
