Overexpression of sterol carrier protein-2 mRNA in patients with cholesterol gallstones

BACKGROUND: Hypersecretion of biliary cholesterol is believed to be one of the important causes of lithogenic bile. Sterol carrier protein-2 ( SCP2 ) participates in choles- terol trafficking and metabolism and may play a key role in cholesterol gallstone formation. This study was undertaken to investigate the expression of liver SCP2 mRNA in pa- tients with cholesterol gallstone and those patients with non-cholesterol gallstone. METHODS: The expression of liver SCP2 mRNA was studi- ed in 36 patients with cholesterol gallstone and 30 patients with non-cholesterol gallstone by reverse transcription-poly- merase chain reaction (RT-PCR). RESULT: The expression of SCP2 mRNA was increased more significantly in patients with cholesterol gallstone than in patients with non-cholesterol gallstone. CONCLUSION: The SCP2 gene was overexpressed in pa- tients with cholesterol gallstone, indicating that SCP2 may be one of the important causes of cholesterol gallstone.

防石中药对家兔胆固醇结石模型成石影响的机理研究

目的:建立家兔胆固醇结石模型,检测家兔肝组织固醇携带蛋白2(Sterol Carrier Protein,SCP2)mRNA的表达及中药对其影响。方法:将42只家兔随机分为正常对照组、高胆固醇膳食组、熊去氧胆酸组、洛伐他丁组、茵陈组和复方中药组。利用高胆固醇膳食诱发家兔胆囊胆固醇结石模型,采用半定量逆转录多聚酶链反应(RT-PCR)检测SCP2mRNA在兔肝组织的表达。结果:4周时处死动物,正常对照组无结石形成;高胆固醇膳食组7只动物有6只形成结石,成石率达85.71%;熊去氧胆酸组成石率为50.00%;洛伐他丁组成石率高达100%;茵陈组和复方中药组成石率较低,均为42.86%。各实验组与对照组相比,SCP2mRNA表达水平均有不同程度升高,各给药组动物肝组织SCP2mRNA表达差异有显著性意义。熊去氧胆酸组和洛伐他丁组SCP2mRNA表达与高胆固醇膳食组相比差异无显著性意义,茵陈组和复方中药组SCP2mRNA表达分别与高胆固醇膳食组相比差异有显著性意义(P<0.05)。除熊去氧胆酸组外,其余各组动物肝组织SCP2mRNA表达均与本组胆汁成石指数(Lithogenicindex,LI)呈正相关。结论:高胆固醇膳食致家兔胆囊结石形成时肝组织SCP2mRNA表达升高。茵陈和复方中药能通过下调SCP2mRNA的表达降低胆固醇结石的发生率;熊去氧胆酸也能降低动物的成石率,但对SCP2mRNA表达没有明显影响。

家兔胆石模型肝组织固醇携带蛋白2表达的意义

目的:复制家兔胆固醇结石模型,检测模型家兔肝组织固醇携带蛋白2(SCP2)mRNA的表达.方法:健康北京大耳白家兔16只,随机分为2组,每组8只:对照组喂普通饮食,模型组喂高胆固醇饮食(含12g/kg胆固醇).4wk时剖杀动物,采用半定量RT-PCR检测SCP2mRNA在家兔肝组织的表达.结果:对照组无结石形成;模型组成石率达87.5%.与对照组相比,模型组动物肝组织SCP2mRNA表达升高(0.79±0.17vs0.44±0.07,P<0.05).结论:高胆固醇膳食致家兔胆囊结石形成时肝组织SCP2mRNA表达升高.