Serine carboxypeptidase-like(SCPL)proteins are a group of acyltransferase enzymes that have important roles in plant growth,development,and stress responses.Although SCPL proteins have been studied in many plants,the ...Serine carboxypeptidase-like(SCPL)proteins are a group of acyltransferase enzymes that have important roles in plant growth,development,and stress responses.Although SCPL proteins have been studied in many plants,the biological functions of SCPL genes in grape are still unknown.In this study,59 putative SCPL proteins were identified from the grape genome.A bioinformatics analysis,including chromosomal locations,exon/intron structures,phylogeny,cis-elements,and conserved motifs,was performed for the gene family.The phylogenetic analysis revealed that VvSCPL proteins could be classified into three groups,with the gene motifs in each group showing high similarity levels.The number of exons in the VvSCPL genes ranged from 1 to 19,suggesting significant variations among grape SCPL genes.The expression of the VvSCPL genes,as assessed by RNA sequencing(RNA-seq)and quantitative real-time PCR,showed that most VvSCPL genes responded to drought-and waterlogging-stress treatments,which indicated their roles in abiotic stress responses.The results provide useful information for further study of SCPL genes in grape.展开更多
Seed weight is a component of seed yield in rapeseed(Brassica napus L.).Although quantitative trait loci(QTL)for seed weight have been reported in rapeseed,only a few causal quantitative trait genes(QTGs)have been ide...Seed weight is a component of seed yield in rapeseed(Brassica napus L.).Although quantitative trait loci(QTL)for seed weight have been reported in rapeseed,only a few causal quantitative trait genes(QTGs)have been identified,resulting in a limitation in understanding of seed weight regulation.We constructed a gene coexpression network at the early seed developmental stage using transcripts of 20,408 genes in QTL intervals and 1017 rapeseed homologs of known genes from other species.Among the 10 modules in this gene coexpression network,modules 1 and 2 were core modules and contained genes involved in source–flow–sink processes such as synthesis and transportation of fatty acid and protein,and photosynthesis.A hub gene SERINE CARBOXYPEPTIDASE-LIKE 19(SCPL19)was identified by candidate gene association analysis in rapeseed and functionally investigated using Arabidopsis T-DNA mutant and overexpression lines.Our study demonstrates the power of gene coexpression analysis to prioritize candidate genes from large candidate QTG sets and enhances the understanding of molecular mechanism for seed weight at the early developmental stage in rapeseed.展开更多
基金This research was supported by the National Key Research and Development Program of China(2018YFD0201300)the Natural Science Funds of Jiangsu Province,China(BK20160587)the China Agriculture Research System of MOF and MARA(CARS-29-11).
文摘Serine carboxypeptidase-like(SCPL)proteins are a group of acyltransferase enzymes that have important roles in plant growth,development,and stress responses.Although SCPL proteins have been studied in many plants,the biological functions of SCPL genes in grape are still unknown.In this study,59 putative SCPL proteins were identified from the grape genome.A bioinformatics analysis,including chromosomal locations,exon/intron structures,phylogeny,cis-elements,and conserved motifs,was performed for the gene family.The phylogenetic analysis revealed that VvSCPL proteins could be classified into three groups,with the gene motifs in each group showing high similarity levels.The number of exons in the VvSCPL genes ranged from 1 to 19,suggesting significant variations among grape SCPL genes.The expression of the VvSCPL genes,as assessed by RNA sequencing(RNA-seq)and quantitative real-time PCR,showed that most VvSCPL genes responded to drought-and waterlogging-stress treatments,which indicated their roles in abiotic stress responses.The results provide useful information for further study of SCPL genes in grape.
基金provided by the National Natural Science Foundation of China(32201776)the Natural Science Foundation of Chongqing(cstc2019jcyj-bsh X0055,cstc2019jcyj-zdxm X0012)。
文摘Seed weight is a component of seed yield in rapeseed(Brassica napus L.).Although quantitative trait loci(QTL)for seed weight have been reported in rapeseed,only a few causal quantitative trait genes(QTGs)have been identified,resulting in a limitation in understanding of seed weight regulation.We constructed a gene coexpression network at the early seed developmental stage using transcripts of 20,408 genes in QTL intervals and 1017 rapeseed homologs of known genes from other species.Among the 10 modules in this gene coexpression network,modules 1 and 2 were core modules and contained genes involved in source–flow–sink processes such as synthesis and transportation of fatty acid and protein,and photosynthesis.A hub gene SERINE CARBOXYPEPTIDASE-LIKE 19(SCPL19)was identified by candidate gene association analysis in rapeseed and functionally investigated using Arabidopsis T-DNA mutant and overexpression lines.Our study demonstrates the power of gene coexpression analysis to prioritize candidate genes from large candidate QTG sets and enhances the understanding of molecular mechanism for seed weight at the early developmental stage in rapeseed.
