Unraveling the distribution of metabolites in traditional Chinese medicine(TCM)provides direct indications for understanding their regulatory and functional basis,which is of paramount significance for better utilizat...Unraveling the distribution of metabolites in traditional Chinese medicine(TCM)provides direct indications for understanding their regulatory and functional basis,which is of paramount significance for better utilization and quality control of medicinal plants[1].Recently,imaging techniques such as near-infrared spectroscopy,Raman spectroscopy,and mass spectrometry(MS)were explored to reveal the spatial context of component accumulation and localization[2,3].展开更多
Background:The study aimed to investigate the protective effect and mechanism of total flavonoids of Scutellaria baicalensis(TFSB)on acute myocardial ischemia(AMI)rats by using functional metabonomics.Methods:Rats wer...Background:The study aimed to investigate the protective effect and mechanism of total flavonoids of Scutellaria baicalensis(TFSB)on acute myocardial ischemia(AMI)rats by using functional metabonomics.Methods:Rats were divided into the Control,Model,AMI positive control(Propranolol hydrochloride,30 mg/kg),low dose TFSB(50 mg/kg),and high dose TFSB(100 mg/kg)groups.Rats received the corresponding treatment by intragastric administration once daily for 10 consecutive days.Electrocardiogram,myocardial enzyme,triphenyltetrazolium chloride staining,hematoxylin-eosin,and enzyme-linked immunosorbent assay were performed to evaluate the protective effect of TFSB on AMI rats.Then,the UHPLC-Q-Orbitrap MS method based on serum metabolomics was utilised to search for metabolic biomarkers and metabolic pathways.Subsequently,Western blot and RT-PCR techniques were employed to identify the respective genes and proteins.Results:Pharmacodynamics revealed that TFSB could ameliorate AMI in rats.The results of the metabolomics analysis indicated that the alterations in metabolic profile observed in rats with AMI were partially improved by treatment with TFSB.Moreover,the mRNA expression levels of 5-lipoxygenase(5-LOX)and 15-lipoxygenase(15-LOX)and the protein expression levels of 5-LOX,15-LOX,interleukin-1β(IL-1β),and NF-κB p65 were reduced following treatment with TFSB.Conclusion:The potential treatment of TFSB in AMI may be ascribed to its ability to regulate arachidonic acid metabolism.展开更多
The seasonal and source variations of flavonoid contents in Scutellariae Radix were investigated by using the materials collected at the same place in Liaoning Province, China, during three years, 1990～1992, and in s...The seasonal and source variations of flavonoid contents in Scutellariae Radix were investigated by using the materials collected at the same place in Liaoning Province, China, during three years, 1990～1992, and in seven other provinces in China. Four principal flavonoids in the plant roots, i.e., baicalin, baicalein, wogonin 7 O glucuronide and wogonin were analyzed by using a reversed phase chromatographic system with a chemically bonded ODS silica gel column and phosphate buffer methanol (68:32 and 1:1) as mobile phase. The contents of the four compounds combined in the herb collected at Chengde, Hebei Province are the highest. The results show that the best time for harvesting the roots in Liaoning Province is the end of August.展开更多
AIM： To investigate the effect of Scutellariae Radix extract （SRE） on ulcerative colitis （UC） in rats induced by dextran-sulfate sodium （DSS）. METHODS： Colitis was induced in male Sprague-Dawley （SD） rats （...AIM： To investigate the effect of Scutellariae Radix extract （SRE） on ulcerative colitis （UC） in rats induced by dextran-sulfate sodium （DSS）. METHODS： Colitis was induced in male Sprague-Dawley （SD） rats （170-180 g） by 4% dextran sulfate sodium （DSS, wt/v, MW 54000） in drinking water for 8 d. The treated rats received 4% DSS and SRE orally （100 mg/kg per day）. Control rats received either tap water or SRE only. Macroscopic assessment which included body weight changes, fecal occult blood and stool consistency were determined daily. At the appointed time, the rats were sacrificed and the entire colons were removed. The colon length and the myeloperoxidase （MPO） activity were measured. The severity of colitis was graded by morphological and histological assessments. The ion transport activity of the colonic mucosa was assessed by electrophysiological technique. RESULTS： Rats treated with oral administration of 4% DSS regularly developed clinical and macroscopic signs of colitis. Treatment with SRE relieved the symptoms, including the reduction in body weight, shortening 2nd ulceration of the colon. Administration of SRE also significantly reduced the histological damage induced by DSS. Moreover, the Isc responses of the colonic mucosa to forskolin, were suppressed after the induction of colitis. The stimulated ion transport activity of DSS-rats treated with SRE displayed significant improvement in the secretory responsiveness. CONCLUSION： SRE was effective in treating acute DSS- induced ulcerative colitis, as gauged by reduced clinical disease, improved macroscopic and histological damage scores, and enhanced recovery of normal colonic secretory function.展开更多
Breast cancer(BCa)is one of the most common malignant tumors that seriously affect women’s physical and mental health and even endanger their lives.Establishing a primary prevention program for BCa remains a challeng...Breast cancer(BCa)is one of the most common malignant tumors that seriously affect women’s physical and mental health and even endanger their lives.Establishing a primary prevention program for BCa remains a challenge.Traditional Chinese medicine,Scutellariae Radix(SR),has been used to treat BCa while its pharmacological mechanism is still unclear.This study aims to reveal the pharmacological mechanism of SR in the treatment of BCa.Chemical constituents of SR were obtained and the targets of the compound were identified via Traditional Chinese Medicine Systems Pharmacology Database(TCMSP).GeneCards and DrugBank databases were used to collect BCa’s related target genes.Disease-compound common target protein interaction network was established by using the STRING database.The network diagrams of the active component-action target and protein-protein interaction(PPI)networks were constructed by Cytoscape software.Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analyses were carried out to further explore the BCa mechanism and therapeutic effect of SR.As a result,121 active compounds were obtained.The Venn diagram results showed that SR and BCa had 57 intersection targets,the more frequently targeted ones are TP53,JUN,CCND1,AKT1,which could affect biological processes such as response to steroid hormone and positive regulation of cell death.KEGG analysis revealed that these targets were related to IL-17 signaling pathway,p53 signaling pathway and PI3K-Akt signaling pathway.In conclusion,this study preliminarily verified the target and mode of action of SR in the treatment of BCa,laying a foundation for further research on its mechanism.展开更多
A novel extraction method of baicalin from radix scutellariae was explored in this study. Liquid-liquid continuous biphasic extraction was a new method, and showed the advantage of high extracting contents of total fl...A novel extraction method of baicalin from radix scutellariae was explored in this study. Liquid-liquid continuous biphasic extraction was a new method, and showed the advantage of high extracting contents of total flavone compared with one of the traditional methods. The novel extraction method which is easy to operate and has good reproducibility was much more effective than the traditional one.展开更多
基金supported by the National Natural Science Foundation of China(Grant No.:81773874).
文摘Unraveling the distribution of metabolites in traditional Chinese medicine(TCM)provides direct indications for understanding their regulatory and functional basis,which is of paramount significance for better utilization and quality control of medicinal plants[1].Recently,imaging techniques such as near-infrared spectroscopy,Raman spectroscopy,and mass spectrometry(MS)were explored to reveal the spatial context of component accumulation and localization[2,3].
基金sponsored by Shandong Provincial Key Research and Development Program(Major Technological Innovation Project)([2021]CXGC010508)Guizhou Province Youth Science and Technology Talent Plan(YQK[2023]038)+1 种基金Science and Technology Department of Zunyi City of Guizhou province of China([2020]7)Key project at central government level:the ability establishment of sustainable use for valuable Chinese medicine resources(2060302).
文摘Background:The study aimed to investigate the protective effect and mechanism of total flavonoids of Scutellaria baicalensis(TFSB)on acute myocardial ischemia(AMI)rats by using functional metabonomics.Methods:Rats were divided into the Control,Model,AMI positive control(Propranolol hydrochloride,30 mg/kg),low dose TFSB(50 mg/kg),and high dose TFSB(100 mg/kg)groups.Rats received the corresponding treatment by intragastric administration once daily for 10 consecutive days.Electrocardiogram,myocardial enzyme,triphenyltetrazolium chloride staining,hematoxylin-eosin,and enzyme-linked immunosorbent assay were performed to evaluate the protective effect of TFSB on AMI rats.Then,the UHPLC-Q-Orbitrap MS method based on serum metabolomics was utilised to search for metabolic biomarkers and metabolic pathways.Subsequently,Western blot and RT-PCR techniques were employed to identify the respective genes and proteins.Results:Pharmacodynamics revealed that TFSB could ameliorate AMI in rats.The results of the metabolomics analysis indicated that the alterations in metabolic profile observed in rats with AMI were partially improved by treatment with TFSB.Moreover,the mRNA expression levels of 5-lipoxygenase(5-LOX)and 15-lipoxygenase(15-LOX)and the protein expression levels of 5-LOX,15-LOX,interleukin-1β(IL-1β),and NF-κB p65 were reduced following treatment with TFSB.Conclusion:The potential treatment of TFSB in AMI may be ascribed to its ability to regulate arachidonic acid metabolism.
文摘The seasonal and source variations of flavonoid contents in Scutellariae Radix were investigated by using the materials collected at the same place in Liaoning Province, China, during three years, 1990～1992, and in seven other provinces in China. Four principal flavonoids in the plant roots, i.e., baicalin, baicalein, wogonin 7 O glucuronide and wogonin were analyzed by using a reversed phase chromatographic system with a chemically bonded ODS silica gel column and phosphate buffer methanol (68:32 and 1:1) as mobile phase. The contents of the four compounds combined in the herb collected at Chengde, Hebei Province are the highest. The results show that the best time for harvesting the roots in Liaoning Province is the end of August.
基金a direct grant for research from The ChineseUniversity of Hong Kong,No. 2041075 awarded to Wing-HungKo
文摘AIM： To investigate the effect of Scutellariae Radix extract （SRE） on ulcerative colitis （UC） in rats induced by dextran-sulfate sodium （DSS）. METHODS： Colitis was induced in male Sprague-Dawley （SD） rats （170-180 g） by 4% dextran sulfate sodium （DSS, wt/v, MW 54000） in drinking water for 8 d. The treated rats received 4% DSS and SRE orally （100 mg/kg per day）. Control rats received either tap water or SRE only. Macroscopic assessment which included body weight changes, fecal occult blood and stool consistency were determined daily. At the appointed time, the rats were sacrificed and the entire colons were removed. The colon length and the myeloperoxidase （MPO） activity were measured. The severity of colitis was graded by morphological and histological assessments. The ion transport activity of the colonic mucosa was assessed by electrophysiological technique. RESULTS： Rats treated with oral administration of 4% DSS regularly developed clinical and macroscopic signs of colitis. Treatment with SRE relieved the symptoms, including the reduction in body weight, shortening 2nd ulceration of the colon. Administration of SRE also significantly reduced the histological damage induced by DSS. Moreover, the Isc responses of the colonic mucosa to forskolin, were suppressed after the induction of colitis. The stimulated ion transport activity of DSS-rats treated with SRE displayed significant improvement in the secretory responsiveness. CONCLUSION： SRE was effective in treating acute DSS- induced ulcerative colitis, as gauged by reduced clinical disease, improved macroscopic and histological damage scores, and enhanced recovery of normal colonic secretory function.
文摘Breast cancer(BCa)is one of the most common malignant tumors that seriously affect women’s physical and mental health and even endanger their lives.Establishing a primary prevention program for BCa remains a challenge.Traditional Chinese medicine,Scutellariae Radix(SR),has been used to treat BCa while its pharmacological mechanism is still unclear.This study aims to reveal the pharmacological mechanism of SR in the treatment of BCa.Chemical constituents of SR were obtained and the targets of the compound were identified via Traditional Chinese Medicine Systems Pharmacology Database(TCMSP).GeneCards and DrugBank databases were used to collect BCa’s related target genes.Disease-compound common target protein interaction network was established by using the STRING database.The network diagrams of the active component-action target and protein-protein interaction(PPI)networks were constructed by Cytoscape software.Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analyses were carried out to further explore the BCa mechanism and therapeutic effect of SR.As a result,121 active compounds were obtained.The Venn diagram results showed that SR and BCa had 57 intersection targets,the more frequently targeted ones are TP53,JUN,CCND1,AKT1,which could affect biological processes such as response to steroid hormone and positive regulation of cell death.KEGG analysis revealed that these targets were related to IL-17 signaling pathway,p53 signaling pathway and PI3K-Akt signaling pathway.In conclusion,this study preliminarily verified the target and mode of action of SR in the treatment of BCa,laying a foundation for further research on its mechanism.
文摘A novel extraction method of baicalin from radix scutellariae was explored in this study. Liquid-liquid continuous biphasic extraction was a new method, and showed the advantage of high extracting contents of total flavone compared with one of the traditional methods. The novel extraction method which is easy to operate and has good reproducibility was much more effective than the traditional one.
