为了探究Sdf1-Cxcr4信号在斑马鱼(Danio rerio)后侧线系统(Posterior lateral line system,PLLs)发育中的作用,本研究首先通过原位杂交发现斑马鱼cxcr4b在迁移的侧线原基-Ⅰ和原基-Ⅱ中表达,sdf1a在原基迁移路径上表达。进一步构建了由...为了探究Sdf1-Cxcr4信号在斑马鱼(Danio rerio)后侧线系统(Posterior lateral line system,PLLs)发育中的作用,本研究首先通过原位杂交发现斑马鱼cxcr4b在迁移的侧线原基-Ⅰ和原基-Ⅱ中表达,sdf1a在原基迁移路径上表达。进一步构建了由热激启动子控制的cxcr7b过表达转基因斑马鱼,在不同时间点过表达cxcr7b抑制Sdf1-Cxcr4信号。结果表明:相较于野生型斑马鱼,过表达cxcr7b的斑马鱼的原基-Ⅰ和原基-Ⅱ的迁移明显减慢。但体侧中线神经丘的腹部迁移、间生和针脚神经丘的形成不受过表达cxcr7b的影响。本研究为理解鱼类侧线系统发育调控和多样性产生奠定基础。展开更多
Previous animal studies have shown that stromal cell-derived factor-1 （SDF-1）/CXC chemokine receptor-4 （CXCR4） signaling pathway plays an important role in the targeted migration of bone marrow-derived mesenchymal...Previous animal studies have shown that stromal cell-derived factor-1 （SDF-1）/CXC chemokine receptor-4 （CXCR4） signaling pathway plays an important role in the targeted migration of bone marrow-derived mesenchymal stem cells （BMSCs） to the injured area. In the present study, we aimed to investigate the potential role of chemotactic SDF-1/CXCR4 signaling pathway in the homing of transplanted BMSCs to the injured cochlea after noise-induced hearing loss （NIHL） in a rat model. White noise exposure （110 dB） paradigm was used for hearing loss induction in male rats for 6 hours in 5 days. Distortion-product otoacoustic emission （DPOAE） responses were recorded before the experiment and post noise exposure.Hoechst 33342-labeled BMSCs and CXCR4 antagonist （AMD3100）-treated BMSCs were injected into the rat cochlea through the round window. SDF-1 protein expression in the cochlear tissue was assayed using western blot assay. The number of labeled BMSCs reaching the endolymph was determined after 24 hours.SDF-1 was significantly increased in the cochlear tissue of rats in the noise exposure group than in the control group. The number of Hoechst 33342-labeled BMSCs reaching the endolymph of the cochlea was significantly smaller in the AMD3100-treated BMSCs group than in the normal BMSCs group. Our present findings suggest that the SDF-1/CXCR4 signaling pathway has a critical role in BMSCs migration to the injured cochlea in a rat model of noise-induced hearing loss.展开更多
文摘为了探究Sdf1-Cxcr4信号在斑马鱼(Danio rerio)后侧线系统(Posterior lateral line system,PLLs)发育中的作用,本研究首先通过原位杂交发现斑马鱼cxcr4b在迁移的侧线原基-Ⅰ和原基-Ⅱ中表达,sdf1a在原基迁移路径上表达。进一步构建了由热激启动子控制的cxcr7b过表达转基因斑马鱼,在不同时间点过表达cxcr7b抑制Sdf1-Cxcr4信号。结果表明:相较于野生型斑马鱼,过表达cxcr7b的斑马鱼的原基-Ⅰ和原基-Ⅱ的迁移明显减慢。但体侧中线神经丘的腹部迁移、间生和针脚神经丘的形成不受过表达cxcr7b的影响。本研究为理解鱼类侧线系统发育调控和多样性产生奠定基础。
基金financially supported by the Hearing Disorders Research Center of Shahid Beheshti University of Medical Sciences
文摘Previous animal studies have shown that stromal cell-derived factor-1 （SDF-1）/CXC chemokine receptor-4 （CXCR4） signaling pathway plays an important role in the targeted migration of bone marrow-derived mesenchymal stem cells （BMSCs） to the injured area. In the present study, we aimed to investigate the potential role of chemotactic SDF-1/CXCR4 signaling pathway in the homing of transplanted BMSCs to the injured cochlea after noise-induced hearing loss （NIHL） in a rat model. White noise exposure （110 dB） paradigm was used for hearing loss induction in male rats for 6 hours in 5 days. Distortion-product otoacoustic emission （DPOAE） responses were recorded before the experiment and post noise exposure.Hoechst 33342-labeled BMSCs and CXCR4 antagonist （AMD3100）-treated BMSCs were injected into the rat cochlea through the round window. SDF-1 protein expression in the cochlear tissue was assayed using western blot assay. The number of labeled BMSCs reaching the endolymph was determined after 24 hours.SDF-1 was significantly increased in the cochlear tissue of rats in the noise exposure group than in the control group. The number of Hoechst 33342-labeled BMSCs reaching the endolymph of the cochlea was significantly smaller in the AMD3100-treated BMSCs group than in the normal BMSCs group. Our present findings suggest that the SDF-1/CXCR4 signaling pathway has a critical role in BMSCs migration to the injured cochlea in a rat model of noise-induced hearing loss.
