Quantitative data analysis in single-molecule localization microscopy(SMLM)is crucial for studying cellular functions at the biomolecular level.In the past decade,several quantitative methods were developed for analyz...Quantitative data analysis in single-molecule localization microscopy(SMLM)is crucial for studying cellular functions at the biomolecular level.In the past decade,several quantitative methods were developed for analyzing SMLM data;however,imaging artifacts in SMLM experiments reduce the accuracy of these methods,and these methods were seldom designed as user-friendly tools.Researchers are now trying to overcome these di±culties by developing easyto-use SMLM data analysis software for certain image analysis tasks.But,this kind of software did not pay su±cient attention to the impact of imaging artifacts on the analysis accuracy,and usually contained only one type of analysis task.Therefore,users are still facing di±culties when they want to have the combined use of different types of analysis methods according to the characteristics of their data and their own needs.In this paper,we report an ImageJ plug-in called DecodeSTORM,which not only has a simple GUI for human–computer interaction,but also combines artifact correction with several quantitative analysis methods.DecodeSTORM includes format conversion,channel registration,artifact correction(drift correction and localization¯ltering),quantitative analysis(segmentation and clustering,spatial distribution statistics and colocalization)and visualization.Importantly,these data analysis methods can be combined freely,thus improving the accuracy of quantitative analysis and allowing users to have an optimal combination of methods.We believe DecodeSTORM is a user-friendly and powerful ImageJ plug-in,which provides an easy and accurate data analysis tool for adventurous biologists who are looking for new imaging tools for studying important questions in cell biology.展开更多
近年来,传统的外业调绘模式逐渐向内外业一体化模式转变。本文以地理国情内外业一体化系统为依托,重点研究了ESRI的离线编辑关键技术,介绍了基于ArcGIS Runtime SDK for Android实现的离线编辑功能,以面修形算法为例,在细粒度的几何编...近年来,传统的外业调绘模式逐渐向内外业一体化模式转变。本文以地理国情内外业一体化系统为依托,重点研究了ESRI的离线编辑关键技术,介绍了基于ArcGIS Runtime SDK for Android实现的离线编辑功能,以面修形算法为例,在细粒度的几何编辑基础上实现了常见的外业编辑业务。展开更多
视频监控技术主要用于重要场所安全防护,随着视频监控技术的发展和应用领域的拓展,视频监控技术可用于工业测控系统中实时状态的监测。介绍了在Windows下Microsoft Visual Studio 2010编程环境中,使用海康威视SDK实现上位机软件中网络...视频监控技术主要用于重要场所安全防护,随着视频监控技术的发展和应用领域的拓展,视频监控技术可用于工业测控系统中实时状态的监测。介绍了在Windows下Microsoft Visual Studio 2010编程环境中,使用海康威视SDK实现上位机软件中网络摄像机的视频预览、录像存储、录像回放和云台控制功能的方法,并给出了关键代码。展开更多
基金supported by the National Natural Science Foundation of China(82160345)Key research and development project of Hainan province(ZDYF2021GXJS017)+2 种基金Key Science and Technology Plan Project of Haikou(2021-016)the Start-up Fund from Hainan University(KYQD(ZR)-20022 and KYQD(ZR)-20077)the Student Innovation and Entrepreneurship Project of Biomedical Engineer-ing School,Hainan University(BMECF2D2021001).
文摘Quantitative data analysis in single-molecule localization microscopy(SMLM)is crucial for studying cellular functions at the biomolecular level.In the past decade,several quantitative methods were developed for analyzing SMLM data;however,imaging artifacts in SMLM experiments reduce the accuracy of these methods,and these methods were seldom designed as user-friendly tools.Researchers are now trying to overcome these di±culties by developing easyto-use SMLM data analysis software for certain image analysis tasks.But,this kind of software did not pay su±cient attention to the impact of imaging artifacts on the analysis accuracy,and usually contained only one type of analysis task.Therefore,users are still facing di±culties when they want to have the combined use of different types of analysis methods according to the characteristics of their data and their own needs.In this paper,we report an ImageJ plug-in called DecodeSTORM,which not only has a simple GUI for human–computer interaction,but also combines artifact correction with several quantitative analysis methods.DecodeSTORM includes format conversion,channel registration,artifact correction(drift correction and localization¯ltering),quantitative analysis(segmentation and clustering,spatial distribution statistics and colocalization)and visualization.Importantly,these data analysis methods can be combined freely,thus improving the accuracy of quantitative analysis and allowing users to have an optimal combination of methods.We believe DecodeSTORM is a user-friendly and powerful ImageJ plug-in,which provides an easy and accurate data analysis tool for adventurous biologists who are looking for new imaging tools for studying important questions in cell biology.
文摘视频监控技术主要用于重要场所安全防护,随着视频监控技术的发展和应用领域的拓展,视频监控技术可用于工业测控系统中实时状态的监测。介绍了在Windows下Microsoft Visual Studio 2010编程环境中,使用海康威视SDK实现上位机软件中网络摄像机的视频预览、录像存储、录像回放和云台控制功能的方法,并给出了关键代码。
