A tung oil-based boron-nitrogen coordination polymer(TWE-BN)was specially designed and synthesized as a highly efficient water-based lubricant additive,which has been beneficial to both energy conservation and conduci...A tung oil-based boron-nitrogen coordination polymer(TWE-BN)was specially designed and synthesized as a highly efficient water-based lubricant additive,which has been beneficial to both energy conservation and conducive to environmental protection.Its hydrolysis stability and tribological properties in water were investigated.To better research the lubricating properties,and thus to understand the interaction between the surface and the lubricating additives.Herein,both experimental and theoretical computations based on density functional theory(DFT)were performed.The addition of TWE-BN reduces the water friction coefficient and wear scar diameter,and the maximum non-seizure load increased from 93 to 726 N.Moreover,the anti-corrosion ability on copper was classified as 1b level.The stainless-steel surface was analyzed using scanning electron microscopy(SEM)and X-ray photoelectron spectroscopy(XPS).In hydrolytic stability testing,TWE-BN was better than nitrogen-free tung oil-based lubricant additive(TWE-B)and remained non-hydrolyzed for at least 15 days,implying the feasibility of tung oil-based boron-nitrogen coordination as highly effective and hydrolytic stability lubricant additives.展开更多
The effects of La on some hydrolytic enzyme activities in red soil were studied in incubation and pot culture experiments. In the incubation experiment, La slightly stimulates the activities of urease and acidic phosp...The effects of La on some hydrolytic enzyme activities in red soil were studied in incubation and pot culture experiments. In the incubation experiment, La slightly stimulates the activities of urease and acidic phosphatase in soil and strongly stimulates sucrase activity in soil. In the pot culture experiment, La stimulates the activities of urease, acidic phosphatase and sucrase to different degrees. The stimulative effects of rare earth elements (REE) on hydrolytic enzyme activities in soil may result in increasing yield of crops.展开更多
Lansoprazole degradation is accelerated in both acidic and basic medium in water. The present investigation deals with the hydrolytic degradation of Lansoprazole. Acidic medium degradation show all known impurities an...Lansoprazole degradation is accelerated in both acidic and basic medium in water. The present investigation deals with the hydrolytic degradation of Lansoprazole. Acidic medium degradation show all known impurities and degradation products whereas basic degradation studies show new impurity which has higher molecular weight than Lansoprazole. New impurity was identified, isolated using mass based auto purification system and characterised by 1H NMR, 13C NMR, HMBC, HSQC, NOE, COSY and HRMS experiments. Isolated impurity was showing molecular weight of 467.10, molecular formula of C23H16F3N5OS and its name is 7-(3-Methyl-4-(2,2,2-trifluoroethoxy) pyridin-2-yl)-7H-benzo[4,5]imidazo[2,1-b]benzo[4,5]imidazo[2,1-d][1,3,5]thiadiazine.展开更多
A new recycling hydrolytic-aerobic combined process was developed to treat the high concentration organic wastewater. Simulated wastewater containing 10 g·L^-1 starch with a CODcr value of 10000 mg·L^-1 wasu...A new recycling hydrolytic-aerobic combined process was developed to treat the high concentration organic wastewater. Simulated wastewater containing 10 g·L^-1 starch with a CODcr value of 10000 mg·L^-1 wasused. At first, the hydrolytic degradation and aerobic degradation process were examined in two batch reactors, respectively. In the stand-alone hydrolytic process, starch in the wastewater almost disappeared after 11 h treatment, but CODCr remained as high as 5803mg·L^-1 after two days. In the aerobic process, the biodegradation rate of starch was much slower during the first 11 h than that in the hydrolytic process, although the CODCr removal efficiency reached 89.6% and more than 90% starch could be degraded after 37.5 h. To determine the interaction effects of the two processes, a series of hydrolytic-aerobic combinations were examined in details. Hydrolytic process played an important role in the whole recycle combination process as it could improve the biodegradability of the high concentration starch wastewater. However, from the other experiments, the negative effect of hydrolytic acidification was found in the hydrolytic-aerobic combination, which suggested that the aerobic microorganisms needed time to adapt themselves to the acidic environment. The effect of the degrading time, which was spent in the hydrolytic and aerobic unit, and the number of circulations, with which the wastewater went through the two units were investigated. It was found that a recycle combination of 6 h hydrolytic process with 12 h aerobic process was highly effective and potentially economical, in which the final removal efficiency of CODcr and efficiency of starch degradation reached 94.1% and 98.8%, respectively.展开更多
Zinc(II) complexes of new macrocyclic tetraamines (cyclam derivatives) having a strategically appended phenolate group, have ben examined as catalyst for the hyd rolysis of 4nitrophenyl acetate(NA). It ovas proven th...Zinc(II) complexes of new macrocyclic tetraamines (cyclam derivatives) having a strategically appended phenolate group, have ben examined as catalyst for the hyd rolysis of 4nitrophenyl acetate(NA). It ovas proven that coordinated phenolate can serve as a good nucleophile that efficiently catalyze 4-nitrophenyl acetate(NA) hydrolysis.展开更多
Sponges (Phylum: Porifera) is one of the major groups in the Lakshadweep coral reefs. These sponges harbor diverse bacteria with metabolic potentiality. From biodiversity to biotechnological prospecting, scientific in...Sponges (Phylum: Porifera) is one of the major groups in the Lakshadweep coral reefs. These sponges harbor diverse bacteria with metabolic potentiality. From biodiversity to biotechnological prospecting, scientific investigations related to sponge associated microorganisms have expanded, but remain rather limited to few geographic locations. In this study, culturable bacteria associated with two demosponges viz Dysidea granulosa, Sigmadocia fibulata and the ambient water were screened for commercially important enzymes such as amylase, protease, gelatinase, lipase, deoxyribonucleic, phosphatase and urease. Amylase and phosphatase were the predominant enzymes produced by >80% of sponge-associated bacteria compared to the ambient water. Nearly 50% of the sponge-associated bacteria expressed multiple enzymatic activities (> 4) with variation in the percentage of expression of individual enzymes. More than 65% of the culturable heterotrophic bacteria associated with sponges were Gammaproteobacteria. The order Vibrionales was the main source for multiple enzyme production. Sponge associated bacteria formed more closely related clusters than the water isolates based upon their activity pattern. High recovery of sponge-associated bacteria with multiple enzymatic activities suggest that these versatile bacteria are yet unexploited potential for bioprospecting.展开更多
In this study,the hydrolytic amino acids of Chinese human and Holstein cow milk of different lactation periods were analyzed by the advanced isobaric tags for relative and absolute quantitation(iTRAQ)combined with the...In this study,the hydrolytic amino acids of Chinese human and Holstein cow milk of different lactation periods were analyzed by the advanced isobaric tags for relative and absolute quantitation(iTRAQ)combined with the high performance liquid chromatography-mass spectrometry(HPLC-MS/MS)approach.The total contents of the hydrolytic amino acids in cow colostrum,cow mature milk,human colostrum and human mature milk were 5.00,4.38,2.12 and 2.48 g·L^(-1),respectively.Among these,the contents of the hydrolytic amino acids in cow milk were higher than those in human milk,indicating a decreasing trend with the prolongation of lactation.Additionally,principal component and hierarchical cluster analyses were used to further screen the differentially expressed amino acids.These results enhanced the understanding of the hydrolytic amino acids in cow and human milk across different lactation periods,which could provide potential directions for newborn dairy powder and nutritious supplementary.展开更多
It is well known that fossil fuels are one of the major pollutants that contribute to climate change and there is a need to develop alternatives to their uses.One option is the use of a renewable fuel such as biogas o...It is well known that fossil fuels are one of the major pollutants that contribute to climate change and there is a need to develop alternatives to their uses.One option is the use of a renewable fuel such as biogas obtained from the breaking down of organic matter in the anaerobic digestion process.When biogas is cleaned up to a certain quality,it can substitute the use of natural gas.However,this slow process requires optimization to increase the amount of gas.There are chemical,physical and biological treatments for organic matter to increase the amount and quality of biogas.In the former research,a biological treatment was tested by adding a selection of microorganisms that showed hydrolytic activity to an anaerobic digester for its optimization.First,a selection of microorganisms with the ability to grow in fresh sewage sludge as only nutrient was selected.Then,the lipolytic,cellulolytic and PA(Proteolytic Activity)of microorganisms was tested using selective growth media.Finally,10 samples with the greatest hydrolytic activity were used to evaluate their effects in the increasing of biogas production in the anaerobic process.The results showed that 83%of the microorganisms with the ability to grow in sewage sludge were proteolytic,while the percentage of lipolytic and cellulolytic microorganisms was smaller.Furthermore,it was found that proteolytic microorganisms are the ones that increase biogas yield in a maximum of 80%with a COD(Chemical Oxygen Demand)reduction of 58%.展开更多
The reaction of bis-[2-amino-4-pheny1-5-thiazolyl] disulfide with 5-nitro-salicylaldehyde in absolute ethanol resulted in the formation of a new Schiff base ligand H<sub>2</sub>L (1). Characterization of t...The reaction of bis-[2-amino-4-pheny1-5-thiazolyl] disulfide with 5-nitro-salicylaldehyde in absolute ethanol resulted in the formation of a new Schiff base ligand H<sub>2</sub>L (1). Characterization of the ligand was performed by FT-IR, <sup>1</sup>H NMR, <sup>13</sup>C NMR, UV-Vis, elemental analysis and single crystal X-ray diffraction. The ligand, (1), possesses a disulfide –S–S– bridge of 2.1121 (3) ? length, and the molecule adopts a cis-conformation around this bond. In the crystal structure of (1), an intramolecular O–H···N hydrogen bond with D… A distance of 2.69 (3) ? was present. The reaction of (1) with Co(NO<sub>3</sub>)<sub>2</sub>·6H<sub>2</sub>O and CuCl<sub>2</sub>·2H<sub>2</sub>O in methanol afforded the corresponding metal complexes. The obtained solids were further investigated by elemental analysis and UV-Vis titration that confirmed the formation of [CoL] and [ClCuHL] complexes. However, recrystallizaion of the Co(II) complex in dimethylsulfoxide caused the complete hydrolysis of the imine bond and afforded a Co(II) complex in which two 5-nitro-salicylaldehyde and two DMSO molecules were coordinated to the central metal in an octahedral fashion. This structure (2) was also confirmed by single crystal X-ray analysis.展开更多
A new metallomicelle, formed by Cu2+ Or Zn2+ ion and N-tetradecyl-2-(N-2-hydroxyethyl aminomethyl) imidazol in the presence of cetyltrimethylammonium bromide, was found to possess remarkable catalytic activity to the ...A new metallomicelle, formed by Cu2+ Or Zn2+ ion and N-tetradecyl-2-(N-2-hydroxyethyl aminomethyl) imidazol in the presence of cetyltrimethylammonium bromide, was found to possess remarkable catalytic activity to the hydrolysis of p-nitrophenyl picolinate.展开更多
Green tea catechins are classified as (-)-epi-forms (2R, 3R) or (-)-forms (2S, 3R) with respect to stereoisomerism. The (-)-forms (2S, 3R) in catechin preparations obtained from green tea amounts to approximately 10% ...Green tea catechins are classified as (-)-epi-forms (2R, 3R) or (-)-forms (2S, 3R) with respect to stereoisomerism. The (-)-forms (2S, 3R) in catechin preparations obtained from green tea amounts to approximately 10% of total catechins, whereas the other 90% are (-)-epiforms (2R, 3R). High temperature induces the conversion of (-)-epiforms (2R, 3R) to (-)-forms (2S, 3R). This study investigated the effect of catechin prepa-rations containing 10, 20 and 30% (-)-forms (2S, 3R) on the oxidative and hydrolytic stability of butter. For comparison, butter with δ-tocopherol and BHT and butter without stabilizer were analysed. Butter stability was examined under conditions of refrigeration (8 oC) and freezing (-22 oC) and at temperature of 50 oC and 100 oC. Catechin preparations were more efficient butter stabilizers than BHT and δ-tocopherol. Thermal modification of catechins that led to the genera-tion of 20% of (-) forms (2S, 3R) improved their antioxidative efficacy, but longer treatment lead- ing to the formation of 30% of (-) forms (2S, 3R) decreased their antioxidative activity. The hy-drolytic stability of butter, however, increased as the amount of (-) forms (2S, 3R) increased.展开更多
基金supported by Special Fund of Chinese Central Government for Basic Scientific Research Operations in Commonweal Research Institutes[No.CAFYBB2019SY037]and National Natural Science Foundation of China[No.31901260].
文摘A tung oil-based boron-nitrogen coordination polymer(TWE-BN)was specially designed and synthesized as a highly efficient water-based lubricant additive,which has been beneficial to both energy conservation and conducive to environmental protection.Its hydrolysis stability and tribological properties in water were investigated.To better research the lubricating properties,and thus to understand the interaction between the surface and the lubricating additives.Herein,both experimental and theoretical computations based on density functional theory(DFT)were performed.The addition of TWE-BN reduces the water friction coefficient and wear scar diameter,and the maximum non-seizure load increased from 93 to 726 N.Moreover,the anti-corrosion ability on copper was classified as 1b level.The stainless-steel surface was analyzed using scanning electron microscopy(SEM)and X-ray photoelectron spectroscopy(XPS).In hydrolytic stability testing,TWE-BN was better than nitrogen-free tung oil-based lubricant additive(TWE-B)and remained non-hydrolyzed for at least 15 days,implying the feasibility of tung oil-based boron-nitrogen coordination as highly effective and hydrolytic stability lubricant additives.
文摘The effects of La on some hydrolytic enzyme activities in red soil were studied in incubation and pot culture experiments. In the incubation experiment, La slightly stimulates the activities of urease and acidic phosphatase in soil and strongly stimulates sucrase activity in soil. In the pot culture experiment, La stimulates the activities of urease, acidic phosphatase and sucrase to different degrees. The stimulative effects of rare earth elements (REE) on hydrolytic enzyme activities in soil may result in increasing yield of crops.
文摘Lansoprazole degradation is accelerated in both acidic and basic medium in water. The present investigation deals with the hydrolytic degradation of Lansoprazole. Acidic medium degradation show all known impurities and degradation products whereas basic degradation studies show new impurity which has higher molecular weight than Lansoprazole. New impurity was identified, isolated using mass based auto purification system and characterised by 1H NMR, 13C NMR, HMBC, HSQC, NOE, COSY and HRMS experiments. Isolated impurity was showing molecular weight of 467.10, molecular formula of C23H16F3N5OS and its name is 7-(3-Methyl-4-(2,2,2-trifluoroethoxy) pyridin-2-yl)-7H-benzo[4,5]imidazo[2,1-b]benzo[4,5]imidazo[2,1-d][1,3,5]thiadiazine.
基金Supported by the National Natural Science Foundation of China (No. 20076037).
文摘A new recycling hydrolytic-aerobic combined process was developed to treat the high concentration organic wastewater. Simulated wastewater containing 10 g·L^-1 starch with a CODcr value of 10000 mg·L^-1 wasused. At first, the hydrolytic degradation and aerobic degradation process were examined in two batch reactors, respectively. In the stand-alone hydrolytic process, starch in the wastewater almost disappeared after 11 h treatment, but CODCr remained as high as 5803mg·L^-1 after two days. In the aerobic process, the biodegradation rate of starch was much slower during the first 11 h than that in the hydrolytic process, although the CODCr removal efficiency reached 89.6% and more than 90% starch could be degraded after 37.5 h. To determine the interaction effects of the two processes, a series of hydrolytic-aerobic combinations were examined in details. Hydrolytic process played an important role in the whole recycle combination process as it could improve the biodegradability of the high concentration starch wastewater. However, from the other experiments, the negative effect of hydrolytic acidification was found in the hydrolytic-aerobic combination, which suggested that the aerobic microorganisms needed time to adapt themselves to the acidic environment. The effect of the degrading time, which was spent in the hydrolytic and aerobic unit, and the number of circulations, with which the wastewater went through the two units were investigated. It was found that a recycle combination of 6 h hydrolytic process with 12 h aerobic process was highly effective and potentially economical, in which the final removal efficiency of CODcr and efficiency of starch degradation reached 94.1% and 98.8%, respectively.
文摘Zinc(II) complexes of new macrocyclic tetraamines (cyclam derivatives) having a strategically appended phenolate group, have ben examined as catalyst for the hyd rolysis of 4nitrophenyl acetate(NA). It ovas proven that coordinated phenolate can serve as a good nucleophile that efficiently catalyze 4-nitrophenyl acetate(NA) hydrolysis.
文摘Sponges (Phylum: Porifera) is one of the major groups in the Lakshadweep coral reefs. These sponges harbor diverse bacteria with metabolic potentiality. From biodiversity to biotechnological prospecting, scientific investigations related to sponge associated microorganisms have expanded, but remain rather limited to few geographic locations. In this study, culturable bacteria associated with two demosponges viz Dysidea granulosa, Sigmadocia fibulata and the ambient water were screened for commercially important enzymes such as amylase, protease, gelatinase, lipase, deoxyribonucleic, phosphatase and urease. Amylase and phosphatase were the predominant enzymes produced by >80% of sponge-associated bacteria compared to the ambient water. Nearly 50% of the sponge-associated bacteria expressed multiple enzymatic activities (> 4) with variation in the percentage of expression of individual enzymes. More than 65% of the culturable heterotrophic bacteria associated with sponges were Gammaproteobacteria. The order Vibrionales was the main source for multiple enzyme production. Sponge associated bacteria formed more closely related clusters than the water isolates based upon their activity pattern. High recovery of sponge-associated bacteria with multiple enzymatic activities suggest that these versatile bacteria are yet unexploited potential for bioprospecting.
基金Supported by the National Key R&D Program of China(2018YFC1604302)the"Twelfth-Five Year"National Science and Technology Plan Project(2013BAD18B03)+2 种基金Shenyang Technological Innovation Project(Y17-0-028)Liaoning Revitalization Talents Program(XLYC1902083)Liaoning Distinguished Professor Project。
文摘In this study,the hydrolytic amino acids of Chinese human and Holstein cow milk of different lactation periods were analyzed by the advanced isobaric tags for relative and absolute quantitation(iTRAQ)combined with the high performance liquid chromatography-mass spectrometry(HPLC-MS/MS)approach.The total contents of the hydrolytic amino acids in cow colostrum,cow mature milk,human colostrum and human mature milk were 5.00,4.38,2.12 and 2.48 g·L^(-1),respectively.Among these,the contents of the hydrolytic amino acids in cow milk were higher than those in human milk,indicating a decreasing trend with the prolongation of lactation.Additionally,principal component and hierarchical cluster analyses were used to further screen the differentially expressed amino acids.These results enhanced the understanding of the hydrolytic amino acids in cow and human milk across different lactation periods,which could provide potential directions for newborn dairy powder and nutritious supplementary.
文摘It is well known that fossil fuels are one of the major pollutants that contribute to climate change and there is a need to develop alternatives to their uses.One option is the use of a renewable fuel such as biogas obtained from the breaking down of organic matter in the anaerobic digestion process.When biogas is cleaned up to a certain quality,it can substitute the use of natural gas.However,this slow process requires optimization to increase the amount of gas.There are chemical,physical and biological treatments for organic matter to increase the amount and quality of biogas.In the former research,a biological treatment was tested by adding a selection of microorganisms that showed hydrolytic activity to an anaerobic digester for its optimization.First,a selection of microorganisms with the ability to grow in fresh sewage sludge as only nutrient was selected.Then,the lipolytic,cellulolytic and PA(Proteolytic Activity)of microorganisms was tested using selective growth media.Finally,10 samples with the greatest hydrolytic activity were used to evaluate their effects in the increasing of biogas production in the anaerobic process.The results showed that 83%of the microorganisms with the ability to grow in sewage sludge were proteolytic,while the percentage of lipolytic and cellulolytic microorganisms was smaller.Furthermore,it was found that proteolytic microorganisms are the ones that increase biogas yield in a maximum of 80%with a COD(Chemical Oxygen Demand)reduction of 58%.
文摘The reaction of bis-[2-amino-4-pheny1-5-thiazolyl] disulfide with 5-nitro-salicylaldehyde in absolute ethanol resulted in the formation of a new Schiff base ligand H<sub>2</sub>L (1). Characterization of the ligand was performed by FT-IR, <sup>1</sup>H NMR, <sup>13</sup>C NMR, UV-Vis, elemental analysis and single crystal X-ray diffraction. The ligand, (1), possesses a disulfide –S–S– bridge of 2.1121 (3) ? length, and the molecule adopts a cis-conformation around this bond. In the crystal structure of (1), an intramolecular O–H···N hydrogen bond with D… A distance of 2.69 (3) ? was present. The reaction of (1) with Co(NO<sub>3</sub>)<sub>2</sub>·6H<sub>2</sub>O and CuCl<sub>2</sub>·2H<sub>2</sub>O in methanol afforded the corresponding metal complexes. The obtained solids were further investigated by elemental analysis and UV-Vis titration that confirmed the formation of [CoL] and [ClCuHL] complexes. However, recrystallizaion of the Co(II) complex in dimethylsulfoxide caused the complete hydrolysis of the imine bond and afforded a Co(II) complex in which two 5-nitro-salicylaldehyde and two DMSO molecules were coordinated to the central metal in an octahedral fashion. This structure (2) was also confirmed by single crystal X-ray analysis.
文摘A new metallomicelle, formed by Cu2+ Or Zn2+ ion and N-tetradecyl-2-(N-2-hydroxyethyl aminomethyl) imidazol in the presence of cetyltrimethylammonium bromide, was found to possess remarkable catalytic activity to the hydrolysis of p-nitrophenyl picolinate.
文摘Green tea catechins are classified as (-)-epi-forms (2R, 3R) or (-)-forms (2S, 3R) with respect to stereoisomerism. The (-)-forms (2S, 3R) in catechin preparations obtained from green tea amounts to approximately 10% of total catechins, whereas the other 90% are (-)-epiforms (2R, 3R). High temperature induces the conversion of (-)-epiforms (2R, 3R) to (-)-forms (2S, 3R). This study investigated the effect of catechin prepa-rations containing 10, 20 and 30% (-)-forms (2S, 3R) on the oxidative and hydrolytic stability of butter. For comparison, butter with δ-tocopherol and BHT and butter without stabilizer were analysed. Butter stability was examined under conditions of refrigeration (8 oC) and freezing (-22 oC) and at temperature of 50 oC and 100 oC. Catechin preparations were more efficient butter stabilizers than BHT and δ-tocopherol. Thermal modification of catechins that led to the genera-tion of 20% of (-) forms (2S, 3R) improved their antioxidative efficacy, but longer treatment lead- ing to the formation of 30% of (-) forms (2S, 3R) decreased their antioxidative activity. The hy-drolytic stability of butter, however, increased as the amount of (-) forms (2S, 3R) increased.