In this study, the outstanding biocompatibility of silk fibroin （SF） and the highly efficient anti-bacterial effect of nano silver （NS） were utilized to prepare SF/NS composite film with anti- bacterial property. ...In this study, the outstanding biocompatibility of silk fibroin （SF） and the highly efficient anti-bacterial effect of nano silver （NS） were utilized to prepare SF/NS composite film with anti- bacterial property. The structure and property of the film were characterized. The results showed that the structure of SF in the film was mainly silk I. SF in the film was almost insoluble in water. The tensile strength of film with NS was significantly lower than that of films without NS. When the addition of NS was within the range of 0%-0.6%, the elongation at break had no significant difference. The antibacterial rate of the film on staphylococcus aurens and escherichia coil increased with the amount of NS. The minimum amount of NS in the fdm was O. 1% and the maximum amount was 0.5%.展开更多
To improve the toughness of silk fibroin( SF) films,poly( ethylene glycol-glycerin)( PEGG) was synthesized with ethylene glycol and epichlorohydrin. The SF / PEGG blend films were prepared by casting aqueous solution ...To improve the toughness of silk fibroin( SF) films,poly( ethylene glycol-glycerin)( PEGG) was synthesized with ethylene glycol and epichlorohydrin. The SF / PEGG blend films were prepared by casting aqueous solution and their structures were characterized. The PEGG was in liquid state at room temperature so it will not be a single phrase at blend film. It crosslinked with SF and made it insolubility in water. The results of X-ray diffraction( XRD) indicated that the crystallinity of the SF in the blend films decreased with the content of PEGG increasing. The tensile strength and elongation at break of blend films were measured using an instron tensile tester. The results showed that the tensile strength and elongation at break of blend films were high enough for application.After the blend films were stored at room temperature for 100 d,the crystallinity, the tensile strength and elongation at wet state increased. The blend films are superior to SF films in providing excellent flexibility and mechanical properties in both dry and wet states. Based on the fact that SF has good biocompatibility,the SF /PEGG blend film will offer new options in many different biomedical applications.展开更多
用溶胶-凝胶法制备了纳米二氧化钛再生丝素复合膜,并用扫描电镜(SEM)、X射线(XRD)、热重分析(TGA、DTG)对复合丝素膜的结构与性能进行了表征。结果表明:加入纳米二氧化钛颗粒后再生丝素膜的热转变温度提高;再生丝素的结晶构象由S ilk I...用溶胶-凝胶法制备了纳米二氧化钛再生丝素复合膜,并用扫描电镜(SEM)、X射线(XRD)、热重分析(TGA、DTG)对复合丝素膜的结构与性能进行了表征。结果表明:加入纳米二氧化钛颗粒后再生丝素膜的热转变温度提高;再生丝素的结晶构象由S ilk I向S ilk II转变,结晶度增加。展开更多
从废弃蚕茧及缫丝下脚料中提取纯净丝素蛋白,采用盐溶酶解法制备纳米级丝素蛋白（SFP）,按照不同配比与聚乙烯醇（PVA）采用水相混合后制备出嵌入纳米级丝素蛋白颗粒的聚乙烯醇（SFP in PVA）膜,对膜进行表征并测定其促细胞生长性能。使...从废弃蚕茧及缫丝下脚料中提取纯净丝素蛋白,采用盐溶酶解法制备纳米级丝素蛋白（SFP）,按照不同配比与聚乙烯醇（PVA）采用水相混合后制备出嵌入纳米级丝素蛋白颗粒的聚乙烯醇（SFP in PVA）膜,对膜进行表征并测定其促细胞生长性能。使用60%NaSCN溶解丝素蛋白后,采用α-糜蛋白酶、胰蛋白酶、中性蛋白酶进行酶解筛选试验,通过场发射扫描电镜观察,发现中性蛋白酶酶解得到的SFP粉末尺寸约为80~150 nm,颗粒均匀。红外光谱表征确认了SFP/PVA膜的基团间结合;扫描电镜显示SFP以微细线段状镶嵌在PVA膜中。对镶嵌膜的SFP/PVA进行了10/90、20/80、30/70、40/60四种配比的筛选试验,通过膜的表面观察、水中形态稳定性观测和促CHO细胞生长性能测试（MTT法）,结果显示,SFP/PVA配比为30/70的镶嵌膜表面光滑、水中形态稳定,促进CHO细胞快速黏附和生长性能最优。展开更多
基金National Natural Science Foundations of China，the Priority Academic Program Development of Jiangsu Higher Education Institutions,China
文摘In this study, the outstanding biocompatibility of silk fibroin （SF） and the highly efficient anti-bacterial effect of nano silver （NS） were utilized to prepare SF/NS composite film with anti- bacterial property. The structure and property of the film were characterized. The results showed that the structure of SF in the film was mainly silk I. SF in the film was almost insoluble in water. The tensile strength of film with NS was significantly lower than that of films without NS. When the addition of NS was within the range of 0%-0.6%, the elongation at break had no significant difference. The antibacterial rate of the film on staphylococcus aurens and escherichia coil increased with the amount of NS. The minimum amount of NS in the fdm was O. 1% and the maximum amount was 0.5%.
基金National Natural Science Foundations of China(Nos.51203107,51373114)the Priority Academic Program Development ofJiangsu Higher Education Institutions,ChinaNatural Science Foundation of Jiangsu Province,China(No.BK20131176)
文摘To improve the toughness of silk fibroin( SF) films,poly( ethylene glycol-glycerin)( PEGG) was synthesized with ethylene glycol and epichlorohydrin. The SF / PEGG blend films were prepared by casting aqueous solution and their structures were characterized. The PEGG was in liquid state at room temperature so it will not be a single phrase at blend film. It crosslinked with SF and made it insolubility in water. The results of X-ray diffraction( XRD) indicated that the crystallinity of the SF in the blend films decreased with the content of PEGG increasing. The tensile strength and elongation at break of blend films were measured using an instron tensile tester. The results showed that the tensile strength and elongation at break of blend films were high enough for application.After the blend films were stored at room temperature for 100 d,the crystallinity, the tensile strength and elongation at wet state increased. The blend films are superior to SF films in providing excellent flexibility and mechanical properties in both dry and wet states. Based on the fact that SF has good biocompatibility,the SF /PEGG blend film will offer new options in many different biomedical applications.
文摘从废弃蚕茧及缫丝下脚料中提取纯净丝素蛋白,采用盐溶酶解法制备纳米级丝素蛋白（SFP）,按照不同配比与聚乙烯醇（PVA）采用水相混合后制备出嵌入纳米级丝素蛋白颗粒的聚乙烯醇（SFP in PVA）膜,对膜进行表征并测定其促细胞生长性能。使用60%NaSCN溶解丝素蛋白后,采用α-糜蛋白酶、胰蛋白酶、中性蛋白酶进行酶解筛选试验,通过场发射扫描电镜观察,发现中性蛋白酶酶解得到的SFP粉末尺寸约为80~150 nm,颗粒均匀。红外光谱表征确认了SFP/PVA膜的基团间结合;扫描电镜显示SFP以微细线段状镶嵌在PVA膜中。对镶嵌膜的SFP/PVA进行了10/90、20/80、30/70、40/60四种配比的筛选试验,通过膜的表面观察、水中形态稳定性观测和促CHO细胞生长性能测试（MTT法）,结果显示,SFP/PVA配比为30/70的镶嵌膜表面光滑、水中形态稳定,促进CHO细胞快速黏附和生长性能最优。
