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Roughness characterization and shearing dislocation failure for rock-backfill interface
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作者 Meifeng Cai Zhilou Feng +3 位作者 Qifeng Guo Xiong Yin Minghui Ma Xun Xi 《International Journal of Minerals,Metallurgy and Materials》 SCIE EI CAS CSCD 2024年第6期1167-1176,共10页
Shearing dislocation is a common failure type for rock–backfill interfaces because of backfill sedimentation and rock strata movement in backfill mining goaf.This paper designed a test method for rock–backfill shear... Shearing dislocation is a common failure type for rock–backfill interfaces because of backfill sedimentation and rock strata movement in backfill mining goaf.This paper designed a test method for rock–backfill shearing dislocation.Using digital image techno-logy and three-dimensional(3D)laser morphology scanning techniques,a set of 3D models with rough joint surfaces was established.Further,the mechanical behavior of rock–backfill shearing dislocation was investigated using a direct shear test.The effects of interface roughness on the shear–displacement curve and failure characteristics of rock–backfill specimens were considered.The 3D fractal dimen-sion,profile line joint roughness coefficient(JRC),profile line two-dimensional fractal dimension,and the surface curvature of the frac-tures were obtained.The correlation characterization of surface roughness was then analyzed,and the shear strength could be measured and calculated using JRC.The results showed the following:there were three failure threshold value points in rock–backfill shearing dis-location:30%–50%displacement before the peak,70%–90%displacement before the peak,and 100%displacement before the peak to post-peak,which could be a sign for rock–backfill shearing dislocation failure.The surface JRC could be used to judge the rock–backfill shearing dislocation failure,including post-peak sliding,uniform variations,and gradient change,corresponding to rock–backfill disloca-tion failure on the field site.The research reveals the damage mechanism for rock–backfill complexes based on the free joint surface,fills the gap of existing shearing theoretical systems for isomerism complexes,and provides a theoretical basis for the prevention and control of possible disasters in backfill mining. 展开更多
关键词 rock–backfill ROUGHNESS correlation characterization shearing dislocation interface failure
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Anisotropic time-dependent behaviors of shale under direct shearing and associated empirical creep models
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作者 Yachen Xie Michael Z.Hou +1 位作者 Hejuan Liu Cunbao Li 《Journal of Rock Mechanics and Geotechnical Engineering》 SCIE CSCD 2024年第4期1262-1279,共18页
Understanding the anisotropic creep behaviors of shale under direct shearing is a challenging issue.In this context,we conducted shear-creep and steady-creep tests on shale with five bedding orientations (i.e.0°,... Understanding the anisotropic creep behaviors of shale under direct shearing is a challenging issue.In this context,we conducted shear-creep and steady-creep tests on shale with five bedding orientations (i.e.0°,30°,45°,60°,and 90°),under multiple levels of direct shearing for the first time.The results show that the anisotropic creep of shale exhibits a significant stress-dependent behavior.Under a low shear stress,the creep compliance of shale increases linearly with the logarithm of time at all bedding orientations,and the increase depends on the bedding orientation and creep time.Under high shear stress conditions,the creep compliance of shale is minimal when the bedding orientation is 0°,and the steady-creep rate of shale increases significantly with increasing bedding orientations of 30°,45°,60°,and 90°.The stress-strain values corresponding to the inception of the accelerated creep stage show an increasing and then decreasing trend with the bedding orientation.A semilogarithmic model that could reflect the stress dependence of the steady-creep rate while considering the hardening and damage process is proposed.The model minimizes the deviation of the calculated steady-state creep rate from the observed value and reveals the behavior of the bedding orientation's influence on the steady-creep rate.The applicability of the five classical empirical creep models is quantitatively evaluated.It shows that the logarithmic model can well explain the experimental creep strain and creep rate,and it can accurately predict long-term shear creep deformation.Based on an improved logarithmic model,the variations in creep parameters with shear stress and bedding orientations are discussed.With abovementioned findings,a mathematical method for constructing an anisotropic shear creep model of shale is proposed,which can characterize the nonlinear dependence of the anisotropic shear creep behavior of shale on the bedding orientation. 展开更多
关键词 Rock anisotropy Direct shear creep Creep compliance Steady-creep rate Empirical model Creep constitutive model
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Anisotropic shearing mechanism of Kangding slate:Experimental investigation and numerical analysis
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作者 Ping Liu Quansheng Liu +4 位作者 Penghai Deng Yucong Pan Yiming Lei Chenglei Du Xianqi Xie 《Journal of Rock Mechanics and Geotechnical Engineering》 SCIE CSCD 2024年第5期1487-1504,共18页
The shear mechanical behavior is regarded as an essential factor affecting the stability of the surrounding rocks in underground engineering.The shear strength and failure mechanisms of layered rock are significantly ... The shear mechanical behavior is regarded as an essential factor affecting the stability of the surrounding rocks in underground engineering.The shear strength and failure mechanisms of layered rock are significantly affected by the foliation angles.Direct shear tests were conducted on cubic slate samples with foliation angles of 0°,30°,45°,60°,and 90°.The effect of foliation angles on failure patterns,acoustic emission(AE)characteristics,and shear strength parameters was analyzed.Based on AE characteristics,the slate failure process could be divided into four stages:quiet period,step-like increasing period,dramatic increasing period,and remission period.A new empirical expression of cohesion for layered rock was proposed,which was compared with linear and sinusoidal cohesion expressions based on the results made by this paper and previous experiments.The comparative analysis demonstrated that the new expression has better prediction ability than other expressions.The proposed empirical equation was used for direct shear simulations with the combined finite-discrete element method(FDEM),and it was found to align well with the experimental results.Considering both computational efficiency and accuracy,it was recommended to use a shear rate of 0.01 m/s for FDEM to carry out direct shear simulations.To balance the relationship between the number of elements and the simulation results in the direct shear simulations,the recommended element size is 1 mm. 展开更多
关键词 ANISOTROPY Empirical expression of cohesion foliation angles Combined finite-discrete element method(FDEM) shear rate Element size
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Engineering behaviour of in situ cored deep cement mixed marine deposits subjected to undrained and drained shearing
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作者 Wei Li Chung Yee Kwok 《Journal of Rock Mechanics and Geotechnical Engineering》 SCIE CSCD 2024年第5期1749-1760,共12页
The deep cement mixing(DCM)is used to improve the capacity and reduce the settlement of the soft ground by forming cemented clay columns.The investigation on the mechanical behaviour of the DCM samples is limited to e... The deep cement mixing(DCM)is used to improve the capacity and reduce the settlement of the soft ground by forming cemented clay columns.The investigation on the mechanical behaviour of the DCM samples is limited to either laboratory-prepared samples or in-situ samples under unconfined compression.In this study,a series of drained and undrained triaxial shearing tests was performed on the in-situ cored DCM samples with high cement content to assess their mechanical behaviours.It is found that the drainage condition affects significantly the stiffness,peak and residual strengths of the DCM samples,which is mainly due to the state of excess pore water pressure at different strain levels,i.e.being positive before the peak deviatoric stress and negative after the peak deviatoric stress,in the undrained tests.The slope of the failure envelope changes obviously with the confining pressures,being steeper at lower stress levels and flatter at higher stress levels.The strength parameters,effective cohesion and friction angle obtained from lower stress levels(c′0 andφ′0)are 400 kPa and 58°,respectively,which are deemed to be true for design in most DCM applications where the in-situ stress levels are normally at lower values of 50-200 kPa.Additionally,the computed tomography(CT)scanning system was adopted to visualize the internal structures of DCM samples.It is found that the clay pockets existing inside the DCM samples due to uneven mixing affect markedly their stress-strain behaviour,which is one of the main reasons for the high variability of the DCM samples. 展开更多
关键词 Deep cement mixing(DCM) In-situ cored sample Triaxial shearing Drainage condition Confining pressure Computed tomography(CT)
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非饱和土地基中端承桩对SH波的水平地震响应
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作者 邹新军 杨紫健 吴文兵 《岩土工程学报》 EI CAS CSCD 北大核心 2024年第1期72-80,共9页
为探讨非饱和土地基中SH波作用下端承桩的水平地震响应,先基于一维波动理论得出SH波引起的土层自由场水平振动解,然后考虑非饱和土体动剪切模量随其饱和度的变化特性,采用三维连续介质力学和Euler梁模型建立轴向静荷载下非饱和土-端承... 为探讨非饱和土地基中SH波作用下端承桩的水平地震响应,先基于一维波动理论得出SH波引起的土层自由场水平振动解,然后考虑非饱和土体动剪切模量随其饱和度的变化特性,采用三维连续介质力学和Euler梁模型建立轴向静荷载下非饱和土-端承桩水平耦合振动模型,进而通过引入势函数和分离变量法推导出桩顶水平位移相互作用因子、桩顶水平位移放大因子和桩身曲率比的表达式,经与已有成果对比验证后再经参数分析,获得土体饱和度、桩身长径比和土体黏滞阻尼对桩身水平抗震特性的影响规律:土体饱和度和桩身长径比仅在高频范围对相互作用因子和放大因子有显著影响;土体黏滞阻尼仅在共振频率使得相互作用因子和放大因子分别逐渐增大和减小。 展开更多
关键词 端承桩 非饱和土 sh 水平振动 Euler梁模型
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玉米籽粒皱缩突变体sh2019的分子标记初步定位
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作者 关海英 董瑞 +7 位作者 刘铁山 刘春晓 何春梅 王娟 刘强 徐倩 张茂林 汪黎明 《山东农业科学》 北大核心 2024年第2期8-13,共6页
玉米籽粒作为光合产物的重要贮藏器官,其发育直接影响百粒重和产量。本研究以玉米籽粒皱缩突变体sh2019、野生型玉米自交系Mo17及两者的杂交F_(2)代为材料,进行表型分析、遗传分析和分子标记初步定位。结果表明,突变体sh2019籽粒干瘪,... 玉米籽粒作为光合产物的重要贮藏器官,其发育直接影响百粒重和产量。本研究以玉米籽粒皱缩突变体sh2019、野生型玉米自交系Mo17及两者的杂交F_(2)代为材料,进行表型分析、遗传分析和分子标记初步定位。结果表明,突变体sh2019籽粒干瘪,种皮皱缩,百粒重和出苗率均比野生型显著下降,分别下降43.55%和69.56%。遗传分析发现突变体sh2019的籽粒皱缩表型受1对隐性核基因控制。利用F_(2)分离群体借助集团分离分析法进行的分子标记定位结果表明,sh2019基因被定位于玉米3号染色体上约30.18 Mb的物理距离内。本研究结果可为开展sh2019基因的精细定位及分子标记辅助育种奠定基础。 展开更多
关键词 玉米 籽粒皱缩突变体sh2019 分子标记定位 表型分析 遗传分析
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圆柱结构中的环向SH波及洞体内表面波不存在性讨论
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作者 邓良玉 曹小杉 汝艳 《应用力学学报》 CAS CSCD 北大核心 2024年第2期382-391,共10页
目前有关圆柱结构中的环向SH波研究仅限于实心柱体与圆柱壳体结构。由于无法直接获得圆柱洞体中传播的SH表面波的解析解,对比研究了实心柱体和多种不同类型的圆柱壳体中的环向SH波,从波结构、应变能密度幅值分布与壳体厚度的关系讨论洞... 目前有关圆柱结构中的环向SH波研究仅限于实心柱体与圆柱壳体结构。由于无法直接获得圆柱洞体中传播的SH表面波的解析解,对比研究了实心柱体和多种不同类型的圆柱壳体中的环向SH波,从波结构、应变能密度幅值分布与壳体厚度的关系讨论洞体内SH表面波的存在性。建立柱坐标系下均匀弹性材料与功能梯度材料环向SH波的波动方程;分别求得方程的贝塞尔函数解和幂级数渐近解;进一步计算得到其频散曲线、波结构与应变能密度幅值。结果表明:幂级数方法可用于计算圆柱型结构中的变系数波动方程,且具有较高的精度;圆柱型结构中环向SH波的能量集中在外表面或次外表面,并随着壳体厚度的增加,能量集中现象更为明显;通过应变能密度幅值分布规律推论出圆柱洞体内表面无法传播环向SH表面波。最后,针对均质结构和梯度结构,采用反证法证明了无法得到满足洞体内SH表面波衰减条件的解析解,从而证明了该推论。 展开更多
关键词 环向sh 贝塞尔函数 幂级数渐近解 波结构 应变能密度
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SH波斜入射下水平成层场地地震动特性研究
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作者 陈三红 张郁山 郝明辉 《震灾防御技术》 CSCD 北大核心 2024年第1期88-95,共8页
基于位移场理论和传递矩阵法,建立SH波斜入射下水平成层场地非线性地震反应等效线性化求解方法,研究SH波不同角度入射下场地地表峰值加速度放大系数及反应谱谱比变化规律。研究结果表明,地震波入射角度对场地地表峰值加速度影响明显,在... 基于位移场理论和传递矩阵法,建立SH波斜入射下水平成层场地非线性地震反应等效线性化求解方法,研究SH波不同角度入射下场地地表峰值加速度放大系数及反应谱谱比变化规律。研究结果表明,地震波入射角度对场地地表峰值加速度影响明显,在大多数情况下,地震波斜入射下地表峰值加速度大于垂直入射;地震波斜入射下反应谱特性变化明显,入射角度对高频段反应谱的放大效应显著,且入射角度对不同类型场地的影响周期范围不同,入射角度对Ⅲ类场地周期影响范围明显宽于Ⅱ类场地。 展开更多
关键词 sh 斜入射 反应谱谱比 放大效应
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NO与SH自由基反应机理及动力学研究
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作者 郝昊 周璐 +1 位作者 史韶旭 马红和 《原子与分子物理学报》 北大核心 2024年第6期13-20,共8页
为了探究深度调峰时,燃煤发电机组运行中氮组分与硫组分交互作用对硫组分演化的影响,研究了NO与SH自由基的详细反应机理.采用B3LYP/6-311++G(d,p)方法优化了SH自由基与NO反应路径上各个驻点的几何构型,并通过IRC验证了反应路径的正确性.... 为了探究深度调峰时,燃煤发电机组运行中氮组分与硫组分交互作用对硫组分演化的影响,研究了NO与SH自由基的详细反应机理.采用B3LYP/6-311++G(d,p)方法优化了SH自由基与NO反应路径上各个驻点的几何构型,并通过IRC验证了反应路径的正确性.在CCSD(T)/def2-TZVPP水平上对反应路径上各个驻点进行了能量计算,通过频率矫正和零点能矫正得到了反应在单重态和三重态上的势能面.计算结果表明,反应共有八条反应通道和三种可能产物,分别为P1(SN+OH)、P2(^(3)SO+^(3)NH)、P3(^(3)S+HNO).其中通道(7)(R→^(3)IM8→^(3)IM9→P1)为该反应的优势通道,反应的主要产物为P1,根据传统过渡态理论与变分过渡态理论并结合隧道矫正计算了该反应通道在298~2000 K范围内的反应速率常数,在此温度范围内反应速率常数三参数拟合为k^(CVT/Eckart)=1.203×10^(-2)T^(4.25)exp(-108.29/RT)cm^(3)·molecule^(-1)·s^(-1),具有正温度系数效应.计算得到的速率常数与文献值吻合较好,适用的温度范围更广,所得的动力学参数和热力学数据可以用于燃烧中硫演化机制的建立. 展开更多
关键词 sh自由基 NO 反应机理 速率常数 密度泛函理论 深度调峰
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lncRNA-miR210HG、SH3BGRL3表达在非小细胞肺癌患者中的临床意义
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作者 徐凯伦 邹芳 +3 位作者 孙雪婷 马方旭 张志华 王布 《中南医学科学杂志》 CAS 2024年第1期71-73,101,共4页
目的分析长链非编码RNA(lncRNA)-miR210HG、SH3BGRL3表达在非小细胞肺癌(NSCLC)患者中的临床意义。方法选取76例NSCLC患者,比较癌组织和癌旁组织中lncRNA-miR210HG和SH3BGRL3 mRNA阳性表达率,并将患者分为阳性组和阴性组。比较lncRNA-mi... 目的分析长链非编码RNA(lncRNA)-miR210HG、SH3BGRL3表达在非小细胞肺癌(NSCLC)患者中的临床意义。方法选取76例NSCLC患者,比较癌组织和癌旁组织中lncRNA-miR210HG和SH3BGRL3 mRNA阳性表达率,并将患者分为阳性组和阴性组。比较lncRNA-miR210HG、SH3BGRL3阳性和阴性表达与临床病理特征的关系。比较各组生存情况。结果癌组织中lncRNA-miR210HG、SH3BGRL3表达水平高于癌旁组织(P<0.05)。lncRNA-miR210HG阳性表达者Ⅲ期占比、淋巴结转移占比高于阴性表达者;SH3BGRL3阳性表达者Ⅲ期占比、淋巴结转移占比、低中分化占比高于阴性表达者(P<0.05)。lncRNA-miR210HG、SH3BGRL3阳性组总生存率均低于阴性组(P<0.05)。结论lncRNA-miR210HG、SH3BGRL3在NSCLC癌组织中高表达,不同表达水平者TNM分期、淋巴结转移、分化程度不同。 展开更多
关键词 非小细胞肺癌 lncRNA-miR210HG sh3BGRL3
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老化黑碳颗粒诱导人神经母细胞瘤细胞SH-SY5Y炎症反应及机制
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作者 黄津 王田田 +2 位作者 安静 钟玉芳 尚羽 《上海大学学报(自然科学版)》 CAS CSCD 北大核心 2024年第2期218-228,共11页
以人神经母细胞瘤细胞SH-SY5Y为研究对象,分析被臭氧氧化的黑碳(oxidized black carbon,OBC)颗粒引起的炎症反应以及核因子-κB(nuclear factor-κB,NF-κB)和磷脂酰肌醇3激酶(phosphatidylin-ositol-3-kinase,PI3K)/蛋白激酶B(protein ... 以人神经母细胞瘤细胞SH-SY5Y为研究对象,分析被臭氧氧化的黑碳(oxidized black carbon,OBC)颗粒引起的炎症反应以及核因子-κB(nuclear factor-κB,NF-κB)和磷脂酰肌醇3激酶(phosphatidylin-ositol-3-kinase,PI3K)/蛋白激酶B(protein kinase B,PKB,又称Akt)信号通路的作用.结果显示:随着OBC颗粒染毒质量浓度的增加(5、10、20、40µg/mL),细胞内线粒体跨膜电位(mitochondrial membrane potential,MMP)水平逐渐下降,DNA损伤程度逐渐升高,呈现质量浓度和时间依赖性;细胞外泌白细胞介素-4(interleukin-4,IL-4)水平升高和细胞内肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)基因高表达,说明OBC颗粒会促进SH-SY5Y细胞发生炎症反应;细胞内活性氧(reactive oxygen species,ROS)水平升高,超氧化物歧化酶(superoxide dismutase,SOD)、血红素加氧酶-1(heme oxygenase-1,HO-1)基因表达量升高,且HO-1蛋白水平升高,说明细胞发生氧化应激;胞内NF-κB和PI3K/Akt通路相关蛋白显著变化,表明OBC颗粒激活了NF-κB和PI3K/Akt信号通路.综上,OBC颗粒可诱导SH-SY5Y细胞发生氧化应激造成DNA损伤,促进炎症反应,且对NF-κB和PI3K/Akt信号通路有重要调控作用. 展开更多
关键词 黑碳颗粒 sh-SY5Y细胞 炎症反应 核因子-ΚB
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灵孢多糖对过氧化氢致SH-SY5Y细胞凋亡及线粒体功能障碍的调控
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作者 李雁冰 王记委 +8 位作者 刘晓琴 郭敏芳 牛晓洁 孟涛 苏琴 王瀚斌 杨立志 马存根 尉杰忠 《中国组织工程研究》 CAS 北大核心 2024年第25期4041-4047,共7页
背景:目前研究证实,灵孢多糖可促进神经退行性相关疾病的神经再生。神经退行性疾病的发生与线粒体功能失调密切相关,但灵孢多糖对神经退行性疾病的细胞凋亡及线粒体功能的调控作用尚不明确。目的:探索灵孢多糖对过氧化氢诱导的SH-SY5Y... 背景:目前研究证实,灵孢多糖可促进神经退行性相关疾病的神经再生。神经退行性疾病的发生与线粒体功能失调密切相关,但灵孢多糖对神经退行性疾病的细胞凋亡及线粒体功能的调控作用尚不明确。目的:探索灵孢多糖对过氧化氢诱导的SH-SY5Y细胞凋亡及线粒体功能障碍的调控作用及机制。方法:SH-SY5Y细胞分为3组:对照组,过氧化氢组,灵孢多糖组。对照组细胞正常培养,过氧化氢组细胞用300μmol/L过氧化氢处理24 h,灵孢多糖组先用300μg/μL灵孢多糖干预一两个小时,然后加入300μmol/L过氧化氢干预24 h,干预结束后用JC-1试剂盒检测线粒体膜电位,TUNEL染色试剂盒检测细胞凋亡情况,丙二醛试剂盒和超氧化物歧化酶试剂盒检测丙二醛和超氧化物歧化酶活性,免疫荧光染色法和Western blot法检测凋亡、线粒体动力学相关蛋白的表达。结果与结论:①与对照组相比,过氧化氢组线粒体膜电位和超氧化歧化酶活性显著降低,细胞凋亡率、丙二醇水平显著增高,差异均有显著性意义(P<0.05);与过氧化氢组相比,灵孢多糖组线粒体膜电位和超氧化歧化酶活性显著增高,细胞凋亡率、丙二醛水平显著下降,差异均有显著性意义(P<0.05);②与对照组相比,过氧化氢组促凋亡蛋白Bax和Caspase-3的表达显著增加,抗凋亡蛋白Bcl-2的表达显著下降,差异均有显著性意义(P<0.05);与过氧化氢组相比,灵孢多糖组促凋亡蛋白Bax、Caspase-3的表达显著降低,抗凋亡蛋白Bcl-2的表达显著增加,差异均有显著性意义(P<0.05);③与对照组相比,过氧化氢组线粒体分裂蛋白Fis1与线粒体动力学蛋白p-Drp1的表达均显著增高,线粒体融合蛋白OPA1、Mfn1、Mfn2的表达均显著降低,差异均有显著性意义(P<0.05);与过氧化氢组相比,灵孢多糖组线粒体分裂蛋白Fis1与线粒体动力学蛋白p-Drp1的表达显著降低,线粒体融合蛋白OPA1、Mfn1、Mfn2的表达显著增高,差异均有显著性意义(P<0.05);④以上结果证实,灵孢多糖可通过改善线粒体功能障碍以减轻过氧化氢诱导的SH-SY5Y细胞氧化应激损伤和细胞凋亡。 展开更多
关键词 灵孢多糖 sh-SY5Y细胞 细胞凋亡 线粒体功能障碍 氧化应激
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不同浓度阿达帕林诱导SH-SY5Y细胞的分化及凋亡
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作者 刘娜娜 张俊娇 +2 位作者 张樊 吴聪英 姜玉武 《中国当代儿科杂志》 CAS CSCD 北大核心 2024年第3期282-288,共7页
目的探讨不同浓度阿达帕林对人神经母细胞瘤细胞系SH-SY5Y细胞形态学及功能的影响,以及诱导细胞分化及凋亡的作用。方法将SH-SY5Y细胞分为对照组、低浓度(0.1μM和1μM)阿达帕林组、高浓度(10μM)阿达帕林组。采用延时显微拍摄技术观察S... 目的探讨不同浓度阿达帕林对人神经母细胞瘤细胞系SH-SY5Y细胞形态学及功能的影响,以及诱导细胞分化及凋亡的作用。方法将SH-SY5Y细胞分为对照组、低浓度(0.1μM和1μM)阿达帕林组、高浓度(10μM)阿达帕林组。采用延时显微拍摄技术观察SH-SY5Y细胞形态学变化,采用免疫荧光染色法检测神经元特异性标志物β-微管蛋白Ⅲ和成熟神经元标志物神经丝重链多肽表达,采用多电极阵列记录SH-SY5Y细胞的电生理特征,细胞凋亡检测试剂盒检测细胞凋亡情况。结果低浓度阿达帕林促进SH-SY5Y细胞突起形成,突起之间相互连接形成网络;低浓度阿达帕林处理SH-SY5Y细胞可见自发性放电活动。与对照组比较,1μM阿达帕林组SH-SY5Y细胞β-微管蛋白Ⅲ和神经丝重链多肽表达升高,高浓度阿达帕林组细胞凋亡水平升高(P<0.05)。结论低浓度阿达帕林可诱导SH-SY5Y细胞分化为成熟的功能性神经元,高浓度阿达帕林可诱导SH-SY5Y细胞凋亡。 展开更多
关键词 阿达帕林 分化 凋亡 sh-SY5Y细胞
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佛手多糖对1-甲基-4-苯基-吡啶离子诱导人神经母细胞瘤(SH-SY5Y)细胞损伤的保护作用研究
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作者 陈进炫 龚舒 +3 位作者 刘天开 龚记熠 乙引 刘文华 《食品与发酵工业》 CAS CSCD 北大核心 2024年第8期17-23,共7页
该文探究佛手多糖对1-甲基-4-苯基-吡啶离子(1-methyl-4-phenyl-pyridine ion,MPP+)诱导人神经母细胞瘤(SH-SY5Y)细胞损伤的保护作用及其机制。佛手多糖经大孔吸附树脂AB-8进行纯化。体外培养SH-SY5Y细胞,构建帕金森病(Parkinson′s dis... 该文探究佛手多糖对1-甲基-4-苯基-吡啶离子(1-methyl-4-phenyl-pyridine ion,MPP+)诱导人神经母细胞瘤(SH-SY5Y)细胞损伤的保护作用及其机制。佛手多糖经大孔吸附树脂AB-8进行纯化。体外培养SH-SY5Y细胞,构建帕金森病(Parkinson′s disease,PD)细胞模型,实验分为对照组、MPP+模型组、佛手多糖组。采用噻唑蓝(methye thiazdye telrazlium,MTT)法检测细胞存活率,Hoechst33258染色法观察细胞形态,2′,7′-二氯荧光黄双乙酸盐荧光探针检测细胞活性氧(reactive oxygen species,ROS)水平,JC-1荧光探针法检测线粒体膜电位,蛋白免疫印迹(Western blot)检测磷酸化蛋白激酶B(phosphorylated protein kinase B,p-Akt)、蛋白激酶B(protein kinase B,Akt)、磷酸化细胞外调节蛋白激酶(phosphorylated extracellular regulated protein kinases1/2,p-ERK1/2)和细胞色素c(cytochrome c,Cyt-c)蛋白表达水平。结果表明,佛手多糖的得率4.86%,纯度为44.46%,经过AB-8纯化后,纯度提高到60.81%;与对照组相比,模型组细胞的存活率显著降低,Hoechst33258染色下可见细胞破碎,细胞核皱缩,细胞内ROS显著增加,线粒体膜电位显著降低。与模型组相比,佛手多糖组的细胞存活率显著增加,细胞形态明显得到改善,ROS水平下降,线粒体膜电位升高。Western blot结果显示,佛手多糖能抑制MPP+引起的p-Akt和p-ERK1/2的降低,以及Cyt-c的上升。综上,佛手多糖对MPP+诱导SH-SY5Y细胞损伤具有保护作用,其机制可能是通过调节线粒体ROS的产生和Cyt-c的释放,进而维持线粒体稳态,激活Akt信号通路和ERK信号通路,抑制细胞的凋亡,从而起到保护作用。研究结果可为缓解帕金森病的发生发展提供理论依据,同时也能更好地开发和利用佛手资源。 展开更多
关键词 佛手多糖 提取纯化 MPP+ sh-SY5Y细胞 保护作用
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miR-181a靶向Bcl-2调控氧糖剥夺/再灌注模型诱导的SH-SY5Y神经细胞凋亡
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作者 袁珊 杨玉莹 +2 位作者 许梅梅 胡广泽 高蕊 《石河子大学学报(自然科学版)》 CAS 北大核心 2024年第1期91-100,共10页
目的皮层是响应脑缺血缺氧最为敏感的组织之一,基于前期深度测序技术,我们筛选获得响应脑缺血缺氧应激的皮层区目标基因miR-181a及Bcl-2。本研究旨在SH-SY5Y细胞株氧糖剥夺/复糖复氧模型验证二者靶向调控关系及功能,明确miR-181a—Bcl-... 目的皮层是响应脑缺血缺氧最为敏感的组织之一,基于前期深度测序技术,我们筛选获得响应脑缺血缺氧应激的皮层区目标基因miR-181a及Bcl-2。本研究旨在SH-SY5Y细胞株氧糖剥夺/复糖复氧模型验证二者靶向调控关系及功能,明确miR-181a—Bcl-2调控网络在OGD/R诱导的神经细胞凋亡中的作用。方法采用线栓法构建大鼠缺血缺氧再灌注损伤模型,脑切片TTC染色及行为学评分法评估模型。应用qRT-PCR及Western Blot验证目标基因的表达。生物信息学分析miR-181a与Bcl-2的靶向结合位点并比对结合位点的保守性,双荧光素酶报告基因实验验证miR-181a与Bcl-2靶向结合的特异性。采用OGD/R细胞模型体外模拟脑缺血再灌注损伤,检测凋亡相关蛋白表达及Hoechst荧光染色评估细胞凋亡。结果大鼠大脑中动脉阻塞后miR-181a、Bcl-2表达变化趋势相反。RNA hybird软件预测miR-181a可结合Bcl-2的3′-UTR区,且结合区域高度保守。双荧光素酶报告基因实验发现,相对于Bcl-23′UTR-WT与mimic-NC共转染组,Bcl-23′UTR-WT与miR-181a mimic共转染后的荧光活性更低(P<0.001),而Bcl-2-Mut与miR-181a mimic共转染组,荧光活性无显著差异(P>0.05)。分别用miR-181a的模拟物及抑制物转染OGD/R诱导的SH-SY5Y细胞,miR-181a可以抑制Bcl-2 mRNA及其蛋白的表达水平(P<0.001)。过表达miR-181a显著增加了SH-SY5Y细胞的凋亡(P<0.001),而抑制miR-181a表达可使SH-SY5Y细胞凋亡显著降低(P<0.001)。结论miR-181a可靶向结合Bcl-2,下调miR-181a可通过促进Bcl-2的表达进而抑制SH-SY5Y神经细胞OGD/R损伤诱导的细胞凋亡。 展开更多
关键词 miR-181a BCL-2 sh-SY5Y OGD/R 凋亡
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Experimental investigation on the factors affecting VWF damage based on an in vitro blood-shearing platform
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作者 Xu Mei Lian Hou +2 位作者 Yu-Lin Chen Hao Mei Ying-Ying Zhong 《Biomedical Engineering Communications》 2023年第4期31-38,共8页
With the increasing number of people suffering from heart failure,ventricular assist devices have gradually become an effective way to treat end-stage heart failure.However,the blood damage caused by ventricular assis... With the increasing number of people suffering from heart failure,ventricular assist devices have gradually become an effective way to treat end-stage heart failure.However,the blood damage caused by ventricular assist devices has not been effectively solved,which is an obstacle to its clinical promotion.Most research focused on erythrocyte damage under shear stress,while few researches were conducted on the interaction between blood under shear stress and the induction of von Willebrand factor(VWF)damage.This research used a vortex oscillator blood-shearing platform to conduct in vitro experiments and used immunoblotting to quantify VWF damage in sheared samples to study the laws of shear-induced VWF damage under different shear stress,different exposure times,different blood components,and hemolysis conditions.It was found that VWF damage increased with exposure time and shear stress.At the same time,under lower shear stress,other blood components had little effect on VWF damage,while in a higher shear stress,other blood components would accelerate VWF damage.Hemolysis will also affect VWF damage,and the higher the degree of hemolysis,the higher the rate of VWF degradation in the plasma.The results of this research provide a reference for VWF damage evaluation standards and follow-up research and also guide for improving the design of ventricular assist devices to reduce VWF damage. 展开更多
关键词 VWF damage shear stress exposure time HEMOLYSIS blood-shearing platform
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Mechanical behaviors of backfill-rock composites: Physical shear test and back-analysis
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作者 Jie Xin Quan Jiang +5 位作者 Fengqiang Gong Lang Liu Chang Liu Qiang Liu Yao Yang Pengfei Chen 《Journal of Rock Mechanics and Geotechnical Engineering》 SCIE CSCD 2024年第3期807-827,共21页
The shear behavior of backfill-rock composites is crucial for mine safety and the management of surface subsidence.For exposing the shear failure mechanism of backfill-rock composites,we conducted shear tests on backf... The shear behavior of backfill-rock composites is crucial for mine safety and the management of surface subsidence.For exposing the shear failure mechanism of backfill-rock composites,we conducted shear tests on backfill-rock composites under three constant normal loads,compared with the unfilled rock.To investigate the macro-and meso-failure characteristics of the samples in the shear tests,the cracking behavior of samples was recorded by a high-speed camera and acoustic emission monitoring.In parallel with the experimental test,the numerical models of backfill-rock composites and unfilled rock were established using the discrete element method to analyze the continuous-discontinuous shearing process.Based on the damage mechanics and statistics,a novel shear constitutive model was proposed to describe mechanical behavior.The results show that backfill-rock composites had a special bimodal phenomenon of shearing load-deformation curve,i.e.the first shearing peak corresponded to rock break and the second shearing peak induced by the broken of aeolian sand-cement/fly ash paste backfill.Moreover,the shearing characteristic curves of the backfill-rock composites could be roughly divided into four stages,i.e.the shear failure of the specimens experienced:stage I:stress concentration;stage II:crack propagation;stage III:crack coalescence;stage IV:shearing friction.The numerical simulation shows that the existence of aeolian sand-cement/fly ash paste backfill inevitably altered the coalescence type and failure mode of the specimens and had a strengthening effect on the shear strength of backfillrock composites.Based on damage mechanics and statistics,a shear constitutive model was proposed to describe the shear fracture characteristics of specimens,especially the bimodal phenomenon.Finally,the micro-and meso-mechanisms of shear failure were discussed by combining the micro-test and numerical results.The research can advance the better understanding of the shear behavior of backfill-rock composites and contribute to the safety of mining engineering. 展开更多
关键词 Physical simulation Backfill-rock composites shear failure CRACKING shear constitutive model
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Shear band evolution and acoustic emission characteristics of sandstone containing non-persistent flaws
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作者 Shuting Miao Peng-Zhi Pan +1 位作者 Chuanqing Zhang Lei Huo 《Journal of Rock Mechanics and Geotechnical Engineering》 SCIE CSCD 2024年第2期497-513,共17页
Direct shear tests were conducted on sandstone specimens under different constant normal stresses to study the coalescence of cracks between non-persistent flaws and the shear sliding characteristics of the shear-form... Direct shear tests were conducted on sandstone specimens under different constant normal stresses to study the coalescence of cracks between non-persistent flaws and the shear sliding characteristics of the shear-formed fault.Digital image correlation and acoustic emission(AE)techniques were used to monitor the evolution of shear bands at the rock bridge area and microcracking behaviors.The experimental results revealed that the shear stresses corresponding to the peak and sub-peak in the stressdisplacement curve are significantly affected by the normal stress.Strain localization bands emerged at both the tip of joints and the rock bridge,and their extension and interaction near the peak stress caused a surge in the AE hit rate and a significant decrease in the AE b value.Short and curvilinear strain bands were detected at low normal stress,while high normal stress generally led to more microcracking events and longer coplanar cracks at the rock bridge area.Furthermore,an increase in normal stress resulted in a higher AE count rate and more energetic AE events during friction sliding along the shearformed fault.It was observed that the elastic energy released during the crack coalescence at the prepeak stage was much greater than that released during friction sliding at the post-peak stage.More than 75%of AE events were located in the low-frequency band(0e100 kHz),and this proportion continued to rise with increasing normal stress.Moreover,more AE events of low AF value and high RA value were observed in specimens subjected to high normal stress,indicating that greater normal stress led to more microcracks of shear nature. 展开更多
关键词 shear band evolution Acoustic emission(AE) Crack coalescence Normal stress shear sliding
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Retraction to: Shear strength of extremely altered serpentinites based on degree of saturation (Ankara, Turkey)
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作者 Koray ULAMIŞ 《Journal of Mountain Science》 SCIE CSCD 2024年第4期1446-1446,共1页
The Editor in Chief has retracted this article.After publication,concerns were raised over potentially inconsistent data-for example,the author mentioned residual soil with silt fraction,but Fig.5 shows even the small... The Editor in Chief has retracted this article.After publication,concerns were raised over potentially inconsistent data-for example,the author mentioned residual soil with silt fraction,but Fig.5 shows even the smallest grains are beyond silt grains.Further analysis showed that Fig.6 and Fig.9 show both loose and dense soils exhibiting very similar dilatance behaviour,which is not typical.The author shared raw data,but it was incomplete and could not be verified.The Editor in Chief,therefore,has lost confidence in the findings of this article.The author does not agree to this retraction. 展开更多
关键词 shear saturation smallest
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On the calibration of a shear stress criterion for rock joints to represent the full stress-strain profile
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作者 Akram Deiminiat Jonathan D.Aubertin Yannic Ethier 《Journal of Rock Mechanics and Geotechnical Engineering》 SCIE CSCD 2024年第2期379-392,共14页
Conventional numerical solutions developed to describe the geomechanical behavior of rock interfaces subjected to differential load emphasize peak and residual shear strengths.The detailed analysis of preand post-peak... Conventional numerical solutions developed to describe the geomechanical behavior of rock interfaces subjected to differential load emphasize peak and residual shear strengths.The detailed analysis of preand post-peak shear stress-displacement behavior is central to various time-dependent and dynamic rock mechanic problems such as rockbursts and structural instabilities in highly stressed conditions.The complete stress-displacement surface(CSDS)model was developed to describe analytically the pre-and post-peak behavior of rock interfaces under differential loads.Original formulations of the CSDS model required extensive curve-fitting iterations which limited its practical applicability and transparent integration into engineering tools.The present work proposes modifications to the CSDS model aimed at developing a comprehensive and modern calibration protocol to describe the complete shear stressdisplacement behavior of rock interfaces under differential loads.The proposed update to the CSDS model incorporates the concept of mobilized shear strength to enhance the post-peak formulations.Barton’s concepts of joint roughness coefficient(JRC)and joint compressive strength(JCS)are incorporated to facilitate empirical estimations for peak shear stress and normal closure relations.Triaxial/uniaxial compression test and direct shear test results are used to validate the updated model and exemplify the proposed calibration method.The results illustrate that the revised model successfully predicts the post-peak and complete axial stressestrain and shear stressedisplacement curves for rock joints. 展开更多
关键词 Full shear profile Post-peak shear behavior Rock joint Joint roughness coefficient(JRC) Axial stress-strain curve
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