Objective Here, we explored molecular changes that could potentially mediate healing effects of Gua Sha — a method employed by the Chinese traditional medicine with proven track records of safe and efficient applicat...Objective Here, we explored molecular changes that could potentially mediate healing effects of Gua Sha — a method employed by the Chinese traditional medicine with proven track records of safe and efficient applications dating back to ancient times as well as support from randomized controlled trials performed by modern medical studies — yet remaining almost entirely unexplored by the modern-day high-throughput methods of the-omics sciences.Methods We investigated transcriptome changes occurring shortly after Gua Sha treatment in the whole blood of healthy volunteers using bulk RNA-seq analysis. We applied various analytical tools to identify genes with consistent expression changes in multiple individuals in response to Gua Sha and their networks.Results We found that while the changes were very subtle and individual-specific, we could identify consistent upregulation of three histone genes. Further analysis of the potential regulatory networks of these histone genes revealed the enrichment of functions involved in the immune response and inflammation.Conclusion The significance of these results in the context of potential effects of Gua Sha and the next steps in exploring the molecular mechanisms of action of this technique are discussed.展开更多
Compared with marine facies shale strata,lacustrine shale strata are more complicated in geological conditions,and thus more difficult to explore and develop.To realize economic exploration and development of lacustri...Compared with marine facies shale strata,lacustrine shale strata are more complicated in geological conditions,and thus more difficult to explore and develop.To realize economic exploration and development of lacustrine shale oil,the geological regularities of accumulation and high yield of retained movable petroleum in shale should be understood first.In this work,taking the shale strata of Kong 2 Member and Sha 3 Member in the Paleogene of Huanghua depression in the Bohai Bay Basin as examples,based on the previous joint analysis results of over ten thousand core samples and the latest oil testing,production test and geochemical data of more than 30 horizontal wells,accumulation conditions and models of retained movable petroleum in lacustrine shale were studied comprehensively.The study shows that at moderate organic matter abundance(with TOC from 2%to 4%),shale strata have the best match between oil content and brittleness,and thus are rich in oil and good in fracability.Moderate ancient lake basin size and moderate sediment supply intensity are the internal factors leading to best coupling of organic matter abundance and brittle mineral content in the shale formation.Moderate thermal evolution maturity of Ro of 0.7%–1.0%(at burial depth of 3200 to 4300 m)is the interval where oil generation from thermal evolution and oil adsorption by kerogen in shale layers match best,and retained movable petroleum is high in proportion.Moderate diagenetic evolution stage(3200 to 4300 m in the middle diagenetic stage A)is conducive to the formation of a large number of dissolved pores and organic matter pores,which provide storage space for shale oil enrichment.Moderate development degree of natural fractures(without damaging the shale oil roof and floor sealing conditions)is conducive to the storage,seepage and preservation of shale oil.The research results have overthrown the general understanding that high organic matter abundance,high maturity,and high development degree of natural fractures are conducive to shale oil enrichment,and have guided the comprehensive evaluation of shale oil and gas sweet spots and well deployment in the second member of the Kongdian Formation in the Cangdong sag and the Shahejie Formation in the Qikou sag.Industrial development of the shale oil in Kong 2 Member of the Cangdong sag has made major breakthrough,and important signs of shale oil have been found in Sha 3 Member of the Qikou sag,demonstrating huge exploration potential of lacustrine shale oil.展开更多
The origin of dolomite in Shahejie Formation shale of Jiyang Depression in eastern China were studied by means of petrologic identification, compositional analysis by X-ray diffraction, stable carbon and oxygen isotop...The origin of dolomite in Shahejie Formation shale of Jiyang Depression in eastern China were studied by means of petrologic identification, compositional analysis by X-ray diffraction, stable carbon and oxygen isotopic composition, and trace element and rare earth element analyses. The results show that the development of dolomite is limited in the lacustrine organic rich shale of Shahejie Formation in the study area. Three kinds of dolomite minerals can be identified: primary dolomite(D1), penecontemporaneous dolomite(D2), and ankerite(Ak). D1 has the structure of primary spherical dolomite, high magnesium and high calcium, with order degree of 0.3-0.5, and is characterized by the intracrystalline corrosion and coexistence of secondary enlargement along the outer edge. D2 has the characteristics of secondary enlargement, order degree of 0.5-0.7, high magnesium, high calcium and containing a little iron and manganese elements. Ak is characterized by high order degree of 0.7-0.9, rhombic crystal, high magnesium, high calcium and high iron. The micritic calcite belongs to primary origin on the basis of the carbon and oxygen isotopic compositions and the fractionation characteristics of rare earth elements. According to the oxygen isotopic fractionation equation between paragenetic dolomite and calcite, it is calculated that the formation temperature of dolomite in the shale is between 36.76-45.83 ℃, belonging to lacustrine low-temperature dolomite. Based on the maturation and growth mechanism of primary and penecontemporaneous dolomite crystals, a dolomite diagenetic sequence and the dolomitization process are proposed, which is corresponding to the diagenetic environment of Shahejie Formation shale in the study area.展开更多
随着二维码技术广泛应用于电子票务、银行支票、电子保单等多个领域,二维码的信息泄露和信息篡改等安全问题日益突出.为提高二维码内部信息的安全性能,从对二维码内部信息加密和二维码信息防篡改俩个角度来提高.基于Visual Studio 2008...随着二维码技术广泛应用于电子票务、银行支票、电子保单等多个领域,二维码的信息泄露和信息篡改等安全问题日益突出.为提高二维码内部信息的安全性能,从对二维码内部信息加密和二维码信息防篡改俩个角度来提高.基于Visual Studio 2008 C#平台,设计了一种采用SHA512哈希函数和Rijndael加密算法混合加密的方法,该方法利用Rijndael加密和SHA512数字签名等技术,对Rijndael第一次加密密钥系统随机分配,并对系统随机分配密钥采用二次Rijndael加密防护方法,并通过SHA512对二维码内部信息防篡改校验,达到对二维码信息及其加密密钥的安全保护.在生成QR二维码之前实现了信息加密,并从系统构架、算法原理和实现及安全性能等多个方面进行了测试和分析.分析表明此方法提高了二维码信息的安全性能,达到对密钥高效管理和对信息的多重保护,而在加密后密文信息容量较明文信息有所增加.展开更多
基金supported by the National Natural Science Foundation of China [Grant No. 32170619 to PK and Grant No. 32000462 to FQ]the Research Fund for International Senior Scientists from the National Natural Science Foundation of China [Grant No. 32150710525 to PK]+1 种基金the Natural Science Foundation of Fujian Province,China [Grant No. 2020J02006 to PK]the Scientific Research Funds of Huaqiao University [Grant No. 15BS101 to PK and Grant No. 22BS114 to FQ]。
文摘Objective Here, we explored molecular changes that could potentially mediate healing effects of Gua Sha — a method employed by the Chinese traditional medicine with proven track records of safe and efficient applications dating back to ancient times as well as support from randomized controlled trials performed by modern medical studies — yet remaining almost entirely unexplored by the modern-day high-throughput methods of the-omics sciences.Methods We investigated transcriptome changes occurring shortly after Gua Sha treatment in the whole blood of healthy volunteers using bulk RNA-seq analysis. We applied various analytical tools to identify genes with consistent expression changes in multiple individuals in response to Gua Sha and their networks.Results We found that while the changes were very subtle and individual-specific, we could identify consistent upregulation of three histone genes. Further analysis of the potential regulatory networks of these histone genes revealed the enrichment of functions involved in the immune response and inflammation.Conclusion The significance of these results in the context of potential effects of Gua Sha and the next steps in exploring the molecular mechanisms of action of this technique are discussed.
基金Supported by the PetroChina Science and Technology Major Project(2019E-2601,2018E-11)
文摘Compared with marine facies shale strata,lacustrine shale strata are more complicated in geological conditions,and thus more difficult to explore and develop.To realize economic exploration and development of lacustrine shale oil,the geological regularities of accumulation and high yield of retained movable petroleum in shale should be understood first.In this work,taking the shale strata of Kong 2 Member and Sha 3 Member in the Paleogene of Huanghua depression in the Bohai Bay Basin as examples,based on the previous joint analysis results of over ten thousand core samples and the latest oil testing,production test and geochemical data of more than 30 horizontal wells,accumulation conditions and models of retained movable petroleum in lacustrine shale were studied comprehensively.The study shows that at moderate organic matter abundance(with TOC from 2%to 4%),shale strata have the best match between oil content and brittleness,and thus are rich in oil and good in fracability.Moderate ancient lake basin size and moderate sediment supply intensity are the internal factors leading to best coupling of organic matter abundance and brittle mineral content in the shale formation.Moderate thermal evolution maturity of Ro of 0.7%–1.0%(at burial depth of 3200 to 4300 m)is the interval where oil generation from thermal evolution and oil adsorption by kerogen in shale layers match best,and retained movable petroleum is high in proportion.Moderate diagenetic evolution stage(3200 to 4300 m in the middle diagenetic stage A)is conducive to the formation of a large number of dissolved pores and organic matter pores,which provide storage space for shale oil enrichment.Moderate development degree of natural fractures(without damaging the shale oil roof and floor sealing conditions)is conducive to the storage,seepage and preservation of shale oil.The research results have overthrown the general understanding that high organic matter abundance,high maturity,and high development degree of natural fractures are conducive to shale oil enrichment,and have guided the comprehensive evaluation of shale oil and gas sweet spots and well deployment in the second member of the Kongdian Formation in the Cangdong sag and the Shahejie Formation in the Qikou sag.Industrial development of the shale oil in Kong 2 Member of the Cangdong sag has made major breakthrough,and important signs of shale oil have been found in Sha 3 Member of the Qikou sag,demonstrating huge exploration potential of lacustrine shale oil.
基金Supported by the National Natural Science Foundation of China(Grant Nos.42172153,41802172)Sinopec Key Laboratory Project(Grant No.KL21042)Shengli Oilfield Company Project(Grant No.YKS2101)。
文摘The origin of dolomite in Shahejie Formation shale of Jiyang Depression in eastern China were studied by means of petrologic identification, compositional analysis by X-ray diffraction, stable carbon and oxygen isotopic composition, and trace element and rare earth element analyses. The results show that the development of dolomite is limited in the lacustrine organic rich shale of Shahejie Formation in the study area. Three kinds of dolomite minerals can be identified: primary dolomite(D1), penecontemporaneous dolomite(D2), and ankerite(Ak). D1 has the structure of primary spherical dolomite, high magnesium and high calcium, with order degree of 0.3-0.5, and is characterized by the intracrystalline corrosion and coexistence of secondary enlargement along the outer edge. D2 has the characteristics of secondary enlargement, order degree of 0.5-0.7, high magnesium, high calcium and containing a little iron and manganese elements. Ak is characterized by high order degree of 0.7-0.9, rhombic crystal, high magnesium, high calcium and high iron. The micritic calcite belongs to primary origin on the basis of the carbon and oxygen isotopic compositions and the fractionation characteristics of rare earth elements. According to the oxygen isotopic fractionation equation between paragenetic dolomite and calcite, it is calculated that the formation temperature of dolomite in the shale is between 36.76-45.83 ℃, belonging to lacustrine low-temperature dolomite. Based on the maturation and growth mechanism of primary and penecontemporaneous dolomite crystals, a dolomite diagenetic sequence and the dolomitization process are proposed, which is corresponding to the diagenetic environment of Shahejie Formation shale in the study area.
文摘随着二维码技术广泛应用于电子票务、银行支票、电子保单等多个领域,二维码的信息泄露和信息篡改等安全问题日益突出.为提高二维码内部信息的安全性能,从对二维码内部信息加密和二维码信息防篡改俩个角度来提高.基于Visual Studio 2008 C#平台,设计了一种采用SHA512哈希函数和Rijndael加密算法混合加密的方法,该方法利用Rijndael加密和SHA512数字签名等技术,对Rijndael第一次加密密钥系统随机分配,并对系统随机分配密钥采用二次Rijndael加密防护方法,并通过SHA512对二维码内部信息防篡改校验,达到对二维码信息及其加密密钥的安全保护.在生成QR二维码之前实现了信息加密,并从系统构架、算法原理和实现及安全性能等多个方面进行了测试和分析.分析表明此方法提高了二维码信息的安全性能,达到对密钥高效管理和对信息的多重保护,而在加密后密文信息容量较明文信息有所增加.
