"HoneySweet" is a plum variety developed through genetic engineering to be highly resistant to plum pox potyvirus (PPV) the causal agent of sharka disease that threatens stone-fruit industries world-wide, an..."HoneySweet" is a plum variety developed through genetic engineering to be highly resistant to plum pox potyvirus (PPV) the causal agent of sharka disease that threatens stone-fruit industries world-wide, and most specifically in Europe. Field testing for over 15 years in Europe has demonstrated the stable and durable PPV resistance of “HoneySweet”. Resistance is based on gene silencing whereby the inserted gene induces a natural plant defense mechanism against viruses. This resistance has been transferred to seedlings through cross-hybridization as a single locus dominant trait making it useful as a parent for developing new plum varieties for specific growing areas and markets. “HoneySweet” plums are of high quality and compare well to the quality and nutritional value of conventional plums. “HoneySweet” demonstrates the utilization of genetic engineering to provide safe and effective solutions to important agricultural challenges facing growers, and ultimately consumers.展开更多
Plum pox virus(PPV)causes sharka-the most serious viral disease of stone fruit trees.PPV is wide spread in Europe and Mediterranean Basin,its incidence has been further approved in Asia and both Americas.Nine PPV stra...Plum pox virus(PPV)causes sharka-the most serious viral disease of stone fruit trees.PPV is wide spread in Europe and Mediterranean Basin,its incidence has been further approved in Asia and both Americas.Nine PPV strains have been recognized until now(PPV-D,PPV-M,PPV-Rec,PPV-EA,PPV-C,PPV-T,PPV-W,PPV-CR,and PPV-An),forming molecularly distinct entities,however,only partially differentiable by their biological or epidemiological properties.The most strict virus-host linkages under natural conditions have been detected for strains naturally infecting cherries(PPV-C and PPV-CR).However,although less stringent but still clear host preference is observed also for three epidemiologically most important strains(PPV-D/plum/apricot,PPV-M/peach,and PPV-Rec/plum).So far no genetic marker has been mapped in the PPV genome,which responsibility for the host specificity/preference could be explicitly demonstrated.In this review,we focus on the host preference of three major PPV strains as evidenced by analysis of an extensive dataset of PPV isolates of Slovak and world-wide origin.Together,we discuss several performed relevant experiments and further possible research procedures aimed to better understand the genetic determinants and mechanisms of the host preference of this potyvirus.展开更多
基金This work was supported in part by grants from the European Union,FP7-IRSES-Interest n 269292(2011-2014).
文摘"HoneySweet" is a plum variety developed through genetic engineering to be highly resistant to plum pox potyvirus (PPV) the causal agent of sharka disease that threatens stone-fruit industries world-wide, and most specifically in Europe. Field testing for over 15 years in Europe has demonstrated the stable and durable PPV resistance of “HoneySweet”. Resistance is based on gene silencing whereby the inserted gene induces a natural plant defense mechanism against viruses. This resistance has been transferred to seedlings through cross-hybridization as a single locus dominant trait making it useful as a parent for developing new plum varieties for specific growing areas and markets. “HoneySweet” plums are of high quality and compare well to the quality and nutritional value of conventional plums. “HoneySweet” demonstrates the utilization of genetic engineering to provide safe and effective solutions to important agricultural challenges facing growers, and ultimately consumers.
基金supported by the grants from the Scientific Grant Agency of Ministry of Education and the Slovak Academy of Sciences (2/0001/15)the Slovak Research and Development Agency (APVV-0174-12)
文摘Plum pox virus(PPV)causes sharka-the most serious viral disease of stone fruit trees.PPV is wide spread in Europe and Mediterranean Basin,its incidence has been further approved in Asia and both Americas.Nine PPV strains have been recognized until now(PPV-D,PPV-M,PPV-Rec,PPV-EA,PPV-C,PPV-T,PPV-W,PPV-CR,and PPV-An),forming molecularly distinct entities,however,only partially differentiable by their biological or epidemiological properties.The most strict virus-host linkages under natural conditions have been detected for strains naturally infecting cherries(PPV-C and PPV-CR).However,although less stringent but still clear host preference is observed also for three epidemiologically most important strains(PPV-D/plum/apricot,PPV-M/peach,and PPV-Rec/plum).So far no genetic marker has been mapped in the PPV genome,which responsibility for the host specificity/preference could be explicitly demonstrated.In this review,we focus on the host preference of three major PPV strains as evidenced by analysis of an extensive dataset of PPV isolates of Slovak and world-wide origin.Together,we discuss several performed relevant experiments and further possible research procedures aimed to better understand the genetic determinants and mechanisms of the host preference of this potyvirus.
