Renal interstitial fibrosis(RIF)is the crucial pathway in chronic kidney disease(CKD)leading to the end-stage renal failure.However,the underlying mechanism of Shen Qi Wan(SQW)on RIF is not fully understood.In the cur...Renal interstitial fibrosis(RIF)is the crucial pathway in chronic kidney disease(CKD)leading to the end-stage renal failure.However,the underlying mechanism of Shen Qi Wan(SQW)on RIF is not fully understood.In the current study,we investigated the role of Aquaporin 1(AQP1)in SQW on tubular epithelial-to-mesenchymal transition(EMT).A RIF mouse model induced by adenine and a TGF-β1-stimulated HK-2 cell model were etablished to explore the involvement of AQP 1 in the protective effect of SQW on EMT in vitro and in vivo.Subsequently,the molecular mechanism of SQW on EMT was explored in HK-2 cells with AQP1 knockdown.The results indicated that SQW alleviated kidney injury and renal collagen deposition in the kidneys of mice induced by adenine,increased the protein expression of E-cadherin and AQP1 expression,and decreased the expression of vimentin andα-smooth muscle actin(α-SMA).Similarly,treatmement with SQW-containing serum significantly halted EMT process in TGF-β1 stimulated HK-2 cells.The expression of snail and slug was significantly upregulated in HK-2 cells after knockdown of AQP1.AQP1 knockdown also increased the mRNA expression of vimentin andα-SMA,and decreased the expression of E-cadherin.The protein expression of vimentin increased,while the expression of E-cadherin and CK-18 significantly decreased after AQP1 knockdown in HK-2 cells.These results revealed that AQP1 knockdown promoted EMT.Furthermore,AQP1 knockdown abolished the protective effect of SQW-containing serum on EMT in HK-2 cells.In sum,SQW attentuates EMT process in RIF through upregulation of the expression of AQP1.展开更多
Objective：To study the effect of tuina combined with Jin Gui Shen Qi Decoction on lumbar spinal stenosis. Methods： In the prospective study, seventy outpatients were divided into a treatment and a control group, 36 ...Objective：To study the effect of tuina combined with Jin Gui Shen Qi Decoction on lumbar spinal stenosis. Methods： In the prospective study, seventy outpatients were divided into a treatment and a control group, 36 cases in the treatment group and 34 in the control group. The treatment group was treated with tuina combined with Jin Gui Shen Qi Decoction, but the control group was treated using tuina alone. The therapeutic effect was evaluated using Japanese Orthopaedic Association （JOA） score. Results： No significant difference in JOA score was found between the two groups before treatment （P〉0.05）, but significant improvements were found in both groups after treatment and at the final follow-up （P〈0.05）, and the JOA score in the treatment group was significantly higher than that in the control group （P〈0.05）. After treatment and at the final follow-up, the therapeutic effect in the treatment group was significantly better （P〈0.05）. Conclusion： Tuina combined with Jin GuiShen Qi Decoction was more effective than tuina alone for lumbar spinal stenosis.展开更多
The yield and stereoselectivity of the Diels-Alder reactions between furan(or 2-methylfuran)and different dienophiles are markedly enhanced by the catalysis of anhydrous YbCl_3.
基金the National Natural Science Foundation of China(Nos.81673839 and 82074304)the Young Talents Foundation of Zhejiang Traditional Chinese Medicine Science and Technology Program(No.2019ZQ014)the Science Foundation of Zhejiang Chinese Medical University(No.2020ZZ13).
文摘Renal interstitial fibrosis(RIF)is the crucial pathway in chronic kidney disease(CKD)leading to the end-stage renal failure.However,the underlying mechanism of Shen Qi Wan(SQW)on RIF is not fully understood.In the current study,we investigated the role of Aquaporin 1(AQP1)in SQW on tubular epithelial-to-mesenchymal transition(EMT).A RIF mouse model induced by adenine and a TGF-β1-stimulated HK-2 cell model were etablished to explore the involvement of AQP 1 in the protective effect of SQW on EMT in vitro and in vivo.Subsequently,the molecular mechanism of SQW on EMT was explored in HK-2 cells with AQP1 knockdown.The results indicated that SQW alleviated kidney injury and renal collagen deposition in the kidneys of mice induced by adenine,increased the protein expression of E-cadherin and AQP1 expression,and decreased the expression of vimentin andα-smooth muscle actin(α-SMA).Similarly,treatmement with SQW-containing serum significantly halted EMT process in TGF-β1 stimulated HK-2 cells.The expression of snail and slug was significantly upregulated in HK-2 cells after knockdown of AQP1.AQP1 knockdown also increased the mRNA expression of vimentin andα-SMA,and decreased the expression of E-cadherin.The protein expression of vimentin increased,while the expression of E-cadherin and CK-18 significantly decreased after AQP1 knockdown in HK-2 cells.These results revealed that AQP1 knockdown promoted EMT.Furthermore,AQP1 knockdown abolished the protective effect of SQW-containing serum on EMT in HK-2 cells.In sum,SQW attentuates EMT process in RIF through upregulation of the expression of AQP1.
文摘Objective：To study the effect of tuina combined with Jin Gui Shen Qi Decoction on lumbar spinal stenosis. Methods： In the prospective study, seventy outpatients were divided into a treatment and a control group, 36 cases in the treatment group and 34 in the control group. The treatment group was treated with tuina combined with Jin Gui Shen Qi Decoction, but the control group was treated using tuina alone. The therapeutic effect was evaluated using Japanese Orthopaedic Association （JOA） score. Results： No significant difference in JOA score was found between the two groups before treatment （P〉0.05）, but significant improvements were found in both groups after treatment and at the final follow-up （P〈0.05）, and the JOA score in the treatment group was significantly higher than that in the control group （P〈0.05）. After treatment and at the final follow-up, the therapeutic effect in the treatment group was significantly better （P〈0.05）. Conclusion： Tuina combined with Jin GuiShen Qi Decoction was more effective than tuina alone for lumbar spinal stenosis.
文摘The yield and stereoselectivity of the Diels-Alder reactions between furan(or 2-methylfuran)and different dienophiles are markedly enhanced by the catalysis of anhydrous YbCl_3.
