AIM To explore the mechanism by which micro RNA-155(miR-155) regulates the pathogenesis of experimental colitis.METHODS A luciferase assay was performed to confirm the binding of miR-155 to the SHIP-1 3'-UTR. MiR-...AIM To explore the mechanism by which micro RNA-155(miR-155) regulates the pathogenesis of experimental colitis.METHODS A luciferase assay was performed to confirm the binding of miR-155 to the SHIP-1 3'-UTR. MiR-155 mimics, negative controls and SHIP-1 expression/knockdown vectors were established and then utilized in gain-and loss-of-function studies performed in raw264.7 cells and primary bone marrow-derived macrophages (BMDMs). Thereafter, dextran sulfate sodium(DSS)-induced colitis mouse model with or without antagomiR-155 treatment was established, and the levels of miR-155 and SHIP-1, as well as the pro-inflammatory capabilities, were measured by western blot, quantitative polymerase chain reaction, and immunohistochemistry.RESULTS MiR-155 directly bound to the 3'-UTR of SHIP-1 mRNA and induced a significant decrease in SHIP-1 expression in both raw264.7 cells and primary BMDMs. MiR-155 markedly promoted cell proliferation and proinflammatory secretions including IL-6, TNF-α, IL-1β, and IFN-γ, whereas these effects could be reversed by the restoration of SHIP-1 expression. In vivo studies showed that antagomiR-155 administration could alleviate DSS-induced intestinal inflammation in Balb/c mice. Moreover, significantly increased SHIP-1 expression, as well as decreased Akt activation and inflammatory response, were observed in the antagomiR-155-treated mice.CONCLUSION MiR-155 promotes experimental colitis by repressing SHIP-1 expression. Thus, the inhibition of miR-155 might be a promising strategy for therapy.展开更多
舰船辐射噪声的调制信息中包含了大量有关舰船特征的信息,作为被声呐识别和判断的依据,由此判别目标的类型和速度。传统的DEMON(Detection of Envelope Modulation On Noise)谱分析时,大多采用功率谱分析,在提取调制谱时,效果不太理想,...舰船辐射噪声的调制信息中包含了大量有关舰船特征的信息,作为被声呐识别和判断的依据,由此判别目标的类型和速度。传统的DEMON(Detection of Envelope Modulation On Noise)谱分析时,大多采用功率谱分析,在提取调制谱时,效果不太理想,存在对噪声抑制能力差、提取后的谐波特征不明显等缺点。论文提出了一种基于EMD分解和1(1/2)维谱分析的提取方法,首先对目标辐射噪声信号进行EMD(Empirical Mode Decomposition)分解得到原始信号的若干个固有模态分量(Intrinsic Mode Function,IMF),其次对这些IMF分量进行解调,然后对其中某几个分量作1(1/2)谱分析得到舰船轴频的基频及谐波信息。仿真和对实测数据分析的结果表明,该方法可以有效提取噪声的特征信息,具有良好的应用前景。展开更多
文摘AIM To explore the mechanism by which micro RNA-155(miR-155) regulates the pathogenesis of experimental colitis.METHODS A luciferase assay was performed to confirm the binding of miR-155 to the SHIP-1 3'-UTR. MiR-155 mimics, negative controls and SHIP-1 expression/knockdown vectors were established and then utilized in gain-and loss-of-function studies performed in raw264.7 cells and primary bone marrow-derived macrophages (BMDMs). Thereafter, dextran sulfate sodium(DSS)-induced colitis mouse model with or without antagomiR-155 treatment was established, and the levels of miR-155 and SHIP-1, as well as the pro-inflammatory capabilities, were measured by western blot, quantitative polymerase chain reaction, and immunohistochemistry.RESULTS MiR-155 directly bound to the 3'-UTR of SHIP-1 mRNA and induced a significant decrease in SHIP-1 expression in both raw264.7 cells and primary BMDMs. MiR-155 markedly promoted cell proliferation and proinflammatory secretions including IL-6, TNF-α, IL-1β, and IFN-γ, whereas these effects could be reversed by the restoration of SHIP-1 expression. In vivo studies showed that antagomiR-155 administration could alleviate DSS-induced intestinal inflammation in Balb/c mice. Moreover, significantly increased SHIP-1 expression, as well as decreased Akt activation and inflammatory response, were observed in the antagomiR-155-treated mice.CONCLUSION MiR-155 promotes experimental colitis by repressing SHIP-1 expression. Thus, the inhibition of miR-155 might be a promising strategy for therapy.
文摘舰船辐射噪声的调制信息中包含了大量有关舰船特征的信息,作为被声呐识别和判断的依据,由此判别目标的类型和速度。传统的DEMON(Detection of Envelope Modulation On Noise)谱分析时,大多采用功率谱分析,在提取调制谱时,效果不太理想,存在对噪声抑制能力差、提取后的谐波特征不明显等缺点。论文提出了一种基于EMD分解和1(1/2)维谱分析的提取方法,首先对目标辐射噪声信号进行EMD(Empirical Mode Decomposition)分解得到原始信号的若干个固有模态分量(Intrinsic Mode Function,IMF),其次对这些IMF分量进行解调,然后对其中某几个分量作1(1/2)谱分析得到舰船轴频的基频及谐波信息。仿真和对实测数据分析的结果表明,该方法可以有效提取噪声的特征信息,具有良好的应用前景。
