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蜡质相关转录因子SHN1/WIN1基因的克隆和植物表达载体的构建 被引量:5
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作者 柴凌燕 翁海波 +1 位作者 王冰 史团省 《贵州农业科学》 CAS 北大核心 2010年第4期26-29,共4页
以野生型拟南芥花蕾中提取的总RNA为模板,通过RT-PCR的方法扩增到与角质和蜡质合成相关的转录因子基因,该目的基因片段SHN1/WIN1(SHN1/WAX INDUCER1)约600 bp,将此片段克隆到PMD-19T载体上,经测序分析与Genbank中报道的序列的同源性为1... 以野生型拟南芥花蕾中提取的总RNA为模板,通过RT-PCR的方法扩增到与角质和蜡质合成相关的转录因子基因,该目的基因片段SHN1/WIN1(SHN1/WAX INDUCER1)约600 bp,将此片段克隆到PMD-19T载体上,经测序分析与Genbank中报道的序列的同源性为100%。以植物表达载体PBI121为基础,构建了由组成型启动子CaMV35S调控的SHN1/WIN1基因的植物表达载体PBI121-SHN1/WIN1,为利用SHN1/WIN1基因改变植物角质膜的结构和成分,提高植物抗逆性特别是抗旱性能奠定了物质基础。 展开更多
关键词 shn1/WIN1基因 基因克隆 表达载体
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大豆转录因子GmWIN1-1的鉴定与表达分析
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作者 蔡桂萍 黄旭升 +2 位作者 史先飞 李润植 张莉 《山西农业科学》 2022年第3期296-302,共7页
为挖掘参与大豆胁迫应答的调控基因,研究聚焦大豆转录因子WAX INDUCER1(WIN1/SHN1)家族成员的鉴定和功能分析,以拟南芥AtWIN1编码序列为索引,在大豆转录组数据库中进行Blast比对,鉴定获得1条编码大豆GmWIN1-1转录因子的cDNA序列。通过... 为挖掘参与大豆胁迫应答的调控基因,研究聚焦大豆转录因子WAX INDUCER1(WIN1/SHN1)家族成员的鉴定和功能分析,以拟南芥AtWIN1编码序列为索引,在大豆转录组数据库中进行Blast比对,鉴定获得1条编码大豆GmWIN1-1转录因子的cDNA序列。通过生物信息学方法和qRT-PCR技术分析GmWIN1-1转录因子的序列特征及其在大豆各组织中和干旱胁迫下的表达水平。结果表明,该序列全长570 bp,编码189个氨基酸,蛋白分子质量为21.2 ku,理论等电点为8.45,为亲水性蛋白。GmWIN1-1蛋白二级结构主要元件为无规则卷曲,三级结构建模与模板5wx9.1蛋白氨基酸序列相似性为45%,为单体蛋白。多序列比对揭示GmWIN1-1与AtWIN1相似性最高。保守基序分析表明,WIN1蛋白具有3个基序,即AP2保守基序、中间基序、C端基序。系统发育分析显示,GmWIN1-1与MtWIN1亲缘关系较近。PlantCARE软件预测,GmWIN1-1启动子含有多个响应逆境胁迫的核心顺式元件。实时荧光定量PCR显示,GmWIN1-1在大豆各组织中的表达水平差异显著,在花器官中表达量最高。干旱胁迫条件下,GmWIN1-1的表达水平呈现先小幅度下降而后快速上调再下降的趋势。大豆GmWIN1-1转录因子在大豆各组织中的表达具有时空特异性,并且可能介导大豆干旱胁迫应答的调控。 展开更多
关键词 大豆 转录因子WAX INDUCER1(WIN1/shn1) 基因鉴定 表达分析 干旱胁迫
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Efects of Washing and Drying on Crystal Structure and Pore Size Distribution(PSD) of Zn_4O_(13)C_(24)H_(12) Framework(IRMOF-1) 被引量:1
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作者 Mehrzad Arjmandi Majid Pakizeh 《Acta Metallurgica Sinica(English Letters)》 SCIE EI CAS CSCD 2013年第5期597-601,共5页
In this study, the efects of various methods of washing and drying of MOF-5 nanocrystals on structure formation were investigated. Eight samples of MOF-5 were synthesized under diferent conditions. TGA, XRD and PSD an... In this study, the efects of various methods of washing and drying of MOF-5 nanocrystals on structure formation were investigated. Eight samples of MOF-5 were synthesized under diferent conditions. TGA, XRD and PSD analysis were applied to characterize of the samples. The methods of washing and drying were found to be important in determining the final structure of MOF-5s. MOF-5 with high BET surface area can be obtained by choosing a suitable method of washing and drying. According to the results obtained in this work, it was found that vacuum drying at 425℃ is sufcient to dissolve the MOF-5-DMF. Similar results were obtained by washing method(withCH2Cl2 andCHCl3), when compared with vacuum drying at 425℃ according to XRD test. The pore size distribution of samples 1-5 and 8 were calculated by SHN1 method and results showed that the samples in which solvent vacuum was DMF, have lower pore volume, uniform pore size distribution and the pore size are smaller than samples 3, 4 and 8. It was also found that activated MOF-5 can be converted to its deactivated form prior to drying of the samples. 展开更多
关键词 Metal organic framework(MOF) MOF-5 nanocrystal Characterization shn1 method
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