The response to single heat treatment and Step-down heat (SDH) treatment in vitro of V79 and L cells was studied. Colony-forming ability was assayed in medium after treatment in vitro. Time-response curves were establ...The response to single heat treatment and Step-down heat (SDH) treatment in vitro of V79 and L cells was studied. Colony-forming ability was assayed in medium after treatment in vitro. Time-response curves were established and subjected to Arrhenius analysis. The Arrhenius curves showed inflection points at 43℃ for V79 cells and at 42℃ for L cells. The activation energies were 145 kcal/mole and 400 kcal/mole above and below 43℃ (P<0.05), respectively, for V79 cells, while 160 kcal/mole and 300 kcal/mole above and below 42℃ (P<0.05), respectively, for L cells. Thermosensitivity of L cells are markedly higher than V79 cells. Both V79 and L cells were sensitized by SDH. The SDH effect was characterized by a reduction in shoulder (an addition effect to sublethal damage), an increase in slope (thermosensitization), and the delay and disappearance of thermotolerant 'tail' for V79 and L cells at 45℃ to 40℃ and 44℃ to 42℃ SDH treatment respectively. Particularly, 42℃ to 39℃ or 42℃ to 40℃ SDH for L cells resulted in thermosensitization effect up to a factor of 7.1 or 2.7, respectively. The effect was quantified by thermorsensitization ratio (TSR), defined as T0 single heated/T0SDH-heated. The relative ratio was much higher for V79 than for L cells. Heat killing with SDH characterized by Arrhenius analysis showed that Step-down heating reduced the activation energy for heat killing more than single heating. The decrease of activation energy for L cells was markedly greater than for V79. These data suggest that greater cellular sensitivity under step-down heating conditions may reflect a different mechanism for cell killing.展开更多
文摘The response to single heat treatment and Step-down heat (SDH) treatment in vitro of V79 and L cells was studied. Colony-forming ability was assayed in medium after treatment in vitro. Time-response curves were established and subjected to Arrhenius analysis. The Arrhenius curves showed inflection points at 43℃ for V79 cells and at 42℃ for L cells. The activation energies were 145 kcal/mole and 400 kcal/mole above and below 43℃ (P<0.05), respectively, for V79 cells, while 160 kcal/mole and 300 kcal/mole above and below 42℃ (P<0.05), respectively, for L cells. Thermosensitivity of L cells are markedly higher than V79 cells. Both V79 and L cells were sensitized by SDH. The SDH effect was characterized by a reduction in shoulder (an addition effect to sublethal damage), an increase in slope (thermosensitization), and the delay and disappearance of thermotolerant 'tail' for V79 and L cells at 45℃ to 40℃ and 44℃ to 42℃ SDH treatment respectively. Particularly, 42℃ to 39℃ or 42℃ to 40℃ SDH for L cells resulted in thermosensitization effect up to a factor of 7.1 or 2.7, respectively. The effect was quantified by thermorsensitization ratio (TSR), defined as T0 single heated/T0SDH-heated. The relative ratio was much higher for V79 than for L cells. Heat killing with SDH characterized by Arrhenius analysis showed that Step-down heating reduced the activation energy for heat killing more than single heating. The decrease of activation energy for L cells was markedly greater than for V79. These data suggest that greater cellular sensitivity under step-down heating conditions may reflect a different mechanism for cell killing.
