Extensive gene expression analysis was carried out after a 0, 4, 36, 72, 96 h short interval successive partial hepatectomy (SISPH) was performed. A total of 185 elements were identified as differing by more than two-...Extensive gene expression analysis was carried out after a 0, 4, 36, 72, 96 h short interval successive partial hepatectomy (SISPH) was performed. A total of 185 elements were identified as differing by more than two-fold in their expression levels at one or more time points. Of these 185 elements, 103 were up-regulated, 82 were down-regulated and 86 elements were unreported genes. Quite a few genes were previously unknown to be involved in liver regenera-tion (LR). Using cluster and general analysis, we found that the genes at five time points of the SISPH share eight different types of different expression profiles and eight distinct temporal in-duction or suppression patterns. A comparison of the gene expression in SISPH with that after PH found that 41 genes were specifically altered in SISPH, and 144 genes were simultaneously up-regulated or down-regulated in SISPH and after PH, but they were present in different amounts at the different time points. The conclusions are that (i) microarrays combined with suppressive subtractive hybridization (SSH) can effectively identify genes involved in LR on a large scale; (ii) more genes were up-regulated than down-regulated; (iii) there are fewer abun-dantly expressed genes than those with increased levels of 2-5 fold.展开更多
When ordinary Smoothed Particle Hydrodynamics （SPH） method is used to simulate wave propagation in a wave tank, it is usually observed that the wave height decays and the wave length elongates along the direction of...When ordinary Smoothed Particle Hydrodynamics （SPH） method is used to simulate wave propagation in a wave tank, it is usually observed that the wave height decays and the wave length elongates along the direction of wave propagation. Accompanied with this phenomenon, the pressure under water decays either and shows a big oscillation simultaneously. The reason is the natural potential tensile instability of modeling water motion with ordinary SPH which is caused by particle negative stress in the computation. I＇o deal with the problems, a new sextic kernel function is proposed to reduce this instability. An appropriate smooth length is given and its computation criterion is also suggested. At the same time, a new kind dynamic boundary condition is introduced. Based on these improvements, the new SPH method named stability improved SPH （SISPH） can simulate the wave propagation well. Both the water surface and pressure can be well expressed and the oscillation of pressure is nearly eliminated. Compared with other improved methods, SISPH can truly reveal the physical reality without bringing some new problems in a simple way.展开更多
AIM: To identify the genes differentially expressed in the regenerating rat liver of 0-4-8-12 h short interval successive partial hepatectomy (SISPH) and to analyze their expression profiles.METHODS: Five hundred and ...AIM: To identify the genes differentially expressed in the regenerating rat liver of 0-4-8-12 h short interval successive partial hepatectomy (SISPH) and to analyze their expression profiles.METHODS: Five hundred and fifty-one elements screened from subtractive cDNA libraries were made into a cDNA microarray (cDNA chip). Extensive gene expression analysis following 0-4-8-12 h SISPH was conducted by microarray.RESULTS: One hundred and eighty-three elements were selected, which were either up- or down-regulated more than 2-fold at one or more time points after SISPH. Cluster analysis and generalization analysis showed that there were five distinct temporal patterns of gene expression.Eighty-six genes were unreported, associated with liver regeneration (LR).CONCLUSION: Microarray analysis shows that the down regulated genes are much more than the up-regulated ones in SISPH; the numbers of genes expressed consistently are fewer than that expressed immediately; the genes expressed in high abundance are much fewer than that increased 2-5-fold. The comparison of SISPH with partial hepatectomy (PH) shows that the expression trends of most genes in SISPH and in PH are similar,but the expression of 43 genes is specifically altered in SISPH.展开更多
基金This work was supported by a grant from theNational Natural Science Foundation of China(Grant No.30270673)by a grant from the Zoological Key Subject ofHenan Province(Grant No.PKSO4004).
文摘Extensive gene expression analysis was carried out after a 0, 4, 36, 72, 96 h short interval successive partial hepatectomy (SISPH) was performed. A total of 185 elements were identified as differing by more than two-fold in their expression levels at one or more time points. Of these 185 elements, 103 were up-regulated, 82 were down-regulated and 86 elements were unreported genes. Quite a few genes were previously unknown to be involved in liver regenera-tion (LR). Using cluster and general analysis, we found that the genes at five time points of the SISPH share eight different types of different expression profiles and eight distinct temporal in-duction or suppression patterns. A comparison of the gene expression in SISPH with that after PH found that 41 genes were specifically altered in SISPH, and 144 genes were simultaneously up-regulated or down-regulated in SISPH and after PH, but they were present in different amounts at the different time points. The conclusions are that (i) microarrays combined with suppressive subtractive hybridization (SSH) can effectively identify genes involved in LR on a large scale; (ii) more genes were up-regulated than down-regulated; (iii) there are fewer abun-dantly expressed genes than those with increased levels of 2-5 fold.
基金financially supported by the National Natural Science Foundation of China(Grant Nos.51579038 and 51490672)the National Basic Research Program of China(Grant No.2013CB036101)
文摘When ordinary Smoothed Particle Hydrodynamics （SPH） method is used to simulate wave propagation in a wave tank, it is usually observed that the wave height decays and the wave length elongates along the direction of wave propagation. Accompanied with this phenomenon, the pressure under water decays either and shows a big oscillation simultaneously. The reason is the natural potential tensile instability of modeling water motion with ordinary SPH which is caused by particle negative stress in the computation. I＇o deal with the problems, a new sextic kernel function is proposed to reduce this instability. An appropriate smooth length is given and its computation criterion is also suggested. At the same time, a new kind dynamic boundary condition is introduced. Based on these improvements, the new SPH method named stability improved SPH （SISPH） can simulate the wave propagation well. Both the water surface and pressure can be well expressed and the oscillation of pressure is nearly eliminated. Compared with other improved methods, SISPH can truly reveal the physical reality without bringing some new problems in a simple way.
基金Supported by the National Natural Science Foundation of China,No. 30270673 and National Key Laboratory Funds, China
文摘AIM: To identify the genes differentially expressed in the regenerating rat liver of 0-4-8-12 h short interval successive partial hepatectomy (SISPH) and to analyze their expression profiles.METHODS: Five hundred and fifty-one elements screened from subtractive cDNA libraries were made into a cDNA microarray (cDNA chip). Extensive gene expression analysis following 0-4-8-12 h SISPH was conducted by microarray.RESULTS: One hundred and eighty-three elements were selected, which were either up- or down-regulated more than 2-fold at one or more time points after SISPH. Cluster analysis and generalization analysis showed that there were five distinct temporal patterns of gene expression.Eighty-six genes were unreported, associated with liver regeneration (LR).CONCLUSION: Microarray analysis shows that the down regulated genes are much more than the up-regulated ones in SISPH; the numbers of genes expressed consistently are fewer than that expressed immediately; the genes expressed in high abundance are much fewer than that increased 2-5-fold. The comparison of SISPH with partial hepatectomy (PH) shows that the expression trends of most genes in SISPH and in PH are similar,but the expression of 43 genes is specifically altered in SISPH.
