目的:探讨精氨酸-甘氨酸-天冬氨酸(RGD)修饰对肿瘤抑素19肽(T-19)抗肝癌活性的影响,比较分析T-19及RGD修饰的T-19(RGD-T-19)对肝癌SK-Hep-1细胞增殖、侵袭和迁移能力的影响。方法:用Fmoc固相法合成T-19及RGD-T-19,用高效液相色谱仪和质...目的:探讨精氨酸-甘氨酸-天冬氨酸(RGD)修饰对肿瘤抑素19肽(T-19)抗肝癌活性的影响,比较分析T-19及RGD修饰的T-19(RGD-T-19)对肝癌SK-Hep-1细胞增殖、侵袭和迁移能力的影响。方法:用Fmoc固相法合成T-19及RGD-T-19,用高效液相色谱仪和质谱进行分离、鉴定。常规培养SK-Hep-1细胞,用0、50、100、150、200、250μg/mL的T-19及RGD-T-19分别处理细胞,分为0μg/mL(对照)组、50μg/mL组、100μg/mL组、150μg/mL组、200μg/mL组、250μg/mL组。CCK-8法、克隆形成实验、划痕愈合实验和Tanswell小室实验、WB法和q PCR法分别检测SK-Hep-1细胞的增殖、迁移、侵袭能力,以及环氧合酶-2(COX-2)、基质金属蛋白酶-2(MMP-2)、MMP-9、组织基质金属蛋白酶抑制剂-1(TIMP-1)、TIMP-2蛋白和MMP-1、MMP-2 mRNA的表达。结果:经质谱鉴定,用Fmoc固相法合成的T-19及RGD-T-19纯度高。T-19和RGD-T-19均能显著抑制SK-Hep-1细胞的增殖、迁移、侵袭能力,抑制COX-2蛋白、MMP-2和MMP-9蛋白及mRNA的表达、促进TIMP-1、TIMP-2蛋白的表达(P <0.05, P <0.01, P <0.001),RGD-T-19的抑制或促进效应均明显强于T-19(均P <0.05)。结论:利用Fmoc固相法合成了纯度高、活性好的T-19及RGD-T-19,两种肽均能抑制SK-Hep-1细胞增殖、侵袭和迁移能力,RGD-T-19作用明显强于T-19。展开更多
The Sifangtai and Mingshui formations were continuously cored in the SK-1 n borehole（China Cretaceous Continental Scientific Drilling-SongKe1-the north borehole）.The core is 767.96 m long,and the recovery is 94.7%.T...The Sifangtai and Mingshui formations were continuously cored in the SK-1 n borehole（China Cretaceous Continental Scientific Drilling-SongKe1-the north borehole）.The core is 767.96 m long,and the recovery is 94.7%.The ages of the formations range from middle Campanian to Danian.The sequence and process of lithology-lithofacies and cyclic stratigraphy were described in detail.Eight litho-types compose the Sifangtai Formation,and 15 litho-types compose the Mingshui Formation.Deposition was predominantly in meandering river and lacustrine environments,including 10 microfacies in the Sifangtai Formation and 15 microfacies in the Mingshui Formation.The complete sequence is composed of 535 m-scale cycles（sixth-order cycle）,152 fifth-order cycles,42 fourth-order cycles and five third-order cycles.The centimeter-scale description of the section revealed some previously unknown horizons such as a special type of mudstone,marl,volcanic ash and favorable sand reservoirs in the formations.The new-found evidence is very important for the interpretation of the evolution of the basin,conditions such as lake oxic events,the K/Pg boundary,tectonism in the late sag basin stage,and the reservoir-cap rock assemblages in the shallow stratigraphy.展开更多
文摘目的:探讨精氨酸-甘氨酸-天冬氨酸(RGD)修饰对肿瘤抑素19肽(T-19)抗肝癌活性的影响,比较分析T-19及RGD修饰的T-19(RGD-T-19)对肝癌SK-Hep-1细胞增殖、侵袭和迁移能力的影响。方法:用Fmoc固相法合成T-19及RGD-T-19,用高效液相色谱仪和质谱进行分离、鉴定。常规培养SK-Hep-1细胞,用0、50、100、150、200、250μg/mL的T-19及RGD-T-19分别处理细胞,分为0μg/mL(对照)组、50μg/mL组、100μg/mL组、150μg/mL组、200μg/mL组、250μg/mL组。CCK-8法、克隆形成实验、划痕愈合实验和Tanswell小室实验、WB法和q PCR法分别检测SK-Hep-1细胞的增殖、迁移、侵袭能力,以及环氧合酶-2(COX-2)、基质金属蛋白酶-2(MMP-2)、MMP-9、组织基质金属蛋白酶抑制剂-1(TIMP-1)、TIMP-2蛋白和MMP-1、MMP-2 mRNA的表达。结果:经质谱鉴定,用Fmoc固相法合成的T-19及RGD-T-19纯度高。T-19和RGD-T-19均能显著抑制SK-Hep-1细胞的增殖、迁移、侵袭能力,抑制COX-2蛋白、MMP-2和MMP-9蛋白及mRNA的表达、促进TIMP-1、TIMP-2蛋白的表达(P <0.05, P <0.01, P <0.001),RGD-T-19的抑制或促进效应均明显强于T-19(均P <0.05)。结论:利用Fmoc固相法合成了纯度高、活性好的T-19及RGD-T-19,两种肽均能抑制SK-Hep-1细胞增殖、侵袭和迁移能力,RGD-T-19作用明显强于T-19。
基金supported by the National Basic Research Program of China(Grant No.2012CB822002)the National Natural Science Foundation of China(Grant No.41202072)the "Key Laboratory of Northeast Asia biological evolution and environment of Ministry of Education" platform base construction project
文摘The Sifangtai and Mingshui formations were continuously cored in the SK-1 n borehole（China Cretaceous Continental Scientific Drilling-SongKe1-the north borehole）.The core is 767.96 m long,and the recovery is 94.7%.The ages of the formations range from middle Campanian to Danian.The sequence and process of lithology-lithofacies and cyclic stratigraphy were described in detail.Eight litho-types compose the Sifangtai Formation,and 15 litho-types compose the Mingshui Formation.Deposition was predominantly in meandering river and lacustrine environments,including 10 microfacies in the Sifangtai Formation and 15 microfacies in the Mingshui Formation.The complete sequence is composed of 535 m-scale cycles（sixth-order cycle）,152 fifth-order cycles,42 fourth-order cycles and five third-order cycles.The centimeter-scale description of the section revealed some previously unknown horizons such as a special type of mudstone,marl,volcanic ash and favorable sand reservoirs in the formations.The new-found evidence is very important for the interpretation of the evolution of the basin,conditions such as lake oxic events,the K/Pg boundary,tectonism in the late sag basin stage,and the reservoir-cap rock assemblages in the shallow stratigraphy.
