The bsd-pg（bundle sheath defective pale green） mutant is a novel maize mutation, controlled by a single recessive gene, which was isolated from offspring of maize plantlets regenerated from tissue callus of the maiz...The bsd-pg（bundle sheath defective pale green） mutant is a novel maize mutation, controlled by a single recessive gene, which was isolated from offspring of maize plantlets regenerated from tissue callus of the maize inbred line 501. The characterization was that the biogenesis and development of the chloroplasts was mainly interfered in bundle sheath cells rather than in mesophyll cells. For mapping the bsd-pg, an F2 population was derived from a cross between the mutant bsd-pg and an inbred line Xianzao 17. Using specific locus amplified fragment sequencing（SLAF-Seq） technology, a total of 5 783 polymorphic SLAFs were analysed with 1 771 homozygous alleles between maternal and paternal parents. There were 49 SLAFs, which had a ratio of paternal to maternal alleles of 2：1 in bulked normal lines, and three trait-related candidate regions were obtained on chromosome 1 with a size of 3.945 Mb. For the fine mapping, new simple sequence repeats（SSRs） markers were designed by utilizing information of the B73 genome and the candidate regions were localized a size of 850 934 bp on chromosome 1 between umc1603 and umc1395, including 35 candidate genes. These results provide a foundation for the cloning of bsd-pg by map-based strategy, which is essential for revealing the functional differentiation and coordination of the two cell types, and helps to elucidate a comprehensive understanding of the C4 photosynthesis pathway and related processes in maize leaves.展开更多
【目的】促进甜樱桃育种进程。【方法】利用968个SLAF标记、20个SSR标记和1个S基因标记,对甜樱桃‘8-102’和‘拉宾斯’的100个杂交后代作图。【结果】构建甜樱桃高密度遗传连锁图谱。该图谱945.96 c M,包含8个连锁群,平均标记间距0.96 ...【目的】促进甜樱桃育种进程。【方法】利用968个SLAF标记、20个SSR标记和1个S基因标记,对甜樱桃‘8-102’和‘拉宾斯’的100个杂交后代作图。【结果】构建甜樱桃高密度遗传连锁图谱。该图谱945.96 c M,包含8个连锁群,平均标记间距0.96 c M。该遗传连锁图谱与其他李属遗传图谱之间具有良好的共线性。另外,S基因标记定位到第6连锁群的末端,和其他甜樱桃图谱的S基因定位极为相似。【结论】利用所构建的甜樱桃遗传连锁图谱,可以进行果实性状QTL定位及分子标记开发,加快甜樱桃育种进程。展开更多
Soybean isoflavones are essential secondary metabolites synthesized in the phenylpropanoid pathway and benefit human health. In the present study, highresolution QTL mapping for isoflavone components was performed usi...Soybean isoflavones are essential secondary metabolites synthesized in the phenylpropanoid pathway and benefit human health. In the present study, highresolution QTL mapping for isoflavone components was performed using specific-locus amplified fragment sequencing(SLAF-seq) with a recombinant inbred line(RIL) population(F5:7) derived from a cross between two cultivated soybean varieties, Luheidou 2(LHD2) and Nanhuizao(NHZ). Using a high-density genetic map comprising 3541 SLAF markers and the isoflavone contents of soybean seeds in the 200 lines in four environments, 24 stable QTL were identified for isoflavone components, explaining 4.2%–21.2% of phenotypic variation.Of these QTL, four novel stable QTL(qG8, qMD19, qMG18, and qTIF19) were identified for genistin, malonyldaidzin, malonylgenistin, and total isoflavones, respectively. Gene annotation revealed three genes involved in isoflavone biosynthesis(Gm4CL, GmIFR, and GmCHR) and 13 MYB-like genes within genomic regions corresponding to stable QTL intervals, suggesting candidate genes underlying these loci. Nine epistatic QTL were identified for isoflavone components, explaining 4.7%–15.6% of phenotypic variation. These results will facilitate understanding the genetic basis of isoflavone accumulation in soybean seeds. The stable QTL and tightly linked SLAF markers may be used for markerassisted selection in soybean breeding programs.展开更多
特异长度扩增片段测序(specific length amplified fragment sequencing,SLAF-seq)技术是以第二代测序为基础的一种简化基因组测序技术。本研究简述了SLAF-seq技术流程,综述了该技术在蔬菜分子标记开发、高密度遗传图谱构建、基因定位...特异长度扩增片段测序(specific length amplified fragment sequencing,SLAF-seq)技术是以第二代测序为基础的一种简化基因组测序技术。本研究简述了SLAF-seq技术流程,综述了该技术在蔬菜分子标记开发、高密度遗传图谱构建、基因定位、遗传多样性分析和辅助全基因组测序等方面的应用,讨论了其应用存在的问题,展望了其发展前景。展开更多
To effectively use elite genes on the long arm of rye chromosome 6（the 6RL arm） in wheat breeding programs,precise and fast identification of 6RL chromatin in wheat backgrounds is necessary.PCR-based 6RL-specific ma...To effectively use elite genes on the long arm of rye chromosome 6（the 6RL arm） in wheat breeding programs,precise and fast identification of 6RL chromatin in wheat backgrounds is necessary.PCR-based 6RL-specific markers can facilitate the detection of elite genes on 6RL in wheat breeding.However,only a limited number of 6RL-specific markers have been developed.In the present study.300 new PCR-based 6RL-specific markers were identified using specific length amplified fragment sequencing（SLAF-seq） technology,and were further physically mapped to four regions on the 6RL arm using 6R and 6RL deletion lines.Interestingly,127 of the 300 markers were physically localized to a region from the site between 2.3 and 2.5 to the telomere,the same region where the powdery mildew resistance gene was mapped.In addition,95 of the 300 markers exhibit polymorphisms,which can be used to investigate the diversity of rye 6RL arms.The markers developed in this study can be used to identify given segments of 6RL in wheat backgrounds and accelerate the utilization of elite genes on 6RL in wheat breeding.展开更多
基金supported by the National Natural Science Foundation of China (30700476 and 31071057)the Beijing Natural Science Foundation, China (5083021)
文摘The bsd-pg（bundle sheath defective pale green） mutant is a novel maize mutation, controlled by a single recessive gene, which was isolated from offspring of maize plantlets regenerated from tissue callus of the maize inbred line 501. The characterization was that the biogenesis and development of the chloroplasts was mainly interfered in bundle sheath cells rather than in mesophyll cells. For mapping the bsd-pg, an F2 population was derived from a cross between the mutant bsd-pg and an inbred line Xianzao 17. Using specific locus amplified fragment sequencing（SLAF-Seq） technology, a total of 5 783 polymorphic SLAFs were analysed with 1 771 homozygous alleles between maternal and paternal parents. There were 49 SLAFs, which had a ratio of paternal to maternal alleles of 2：1 in bulked normal lines, and three trait-related candidate regions were obtained on chromosome 1 with a size of 3.945 Mb. For the fine mapping, new simple sequence repeats（SSRs） markers were designed by utilizing information of the B73 genome and the candidate regions were localized a size of 850 934 bp on chromosome 1 between umc1603 and umc1395, including 35 candidate genes. These results provide a foundation for the cloning of bsd-pg by map-based strategy, which is essential for revealing the functional differentiation and coordination of the two cell types, and helps to elucidate a comprehensive understanding of the C4 photosynthesis pathway and related processes in maize leaves.
文摘【目的】促进甜樱桃育种进程。【方法】利用968个SLAF标记、20个SSR标记和1个S基因标记,对甜樱桃‘8-102’和‘拉宾斯’的100个杂交后代作图。【结果】构建甜樱桃高密度遗传连锁图谱。该图谱945.96 c M,包含8个连锁群,平均标记间距0.96 c M。该遗传连锁图谱与其他李属遗传图谱之间具有良好的共线性。另外,S基因标记定位到第6连锁群的末端,和其他甜樱桃图谱的S基因定位极为相似。【结论】利用所构建的甜樱桃遗传连锁图谱,可以进行果实性状QTL定位及分子标记开发,加快甜樱桃育种进程。
基金supported by the National Key Technology R&D Program of China during the Twelfth Five-Year Plan Period of China (2014BAD11B01-x02)Beijing Science and Technology Project (Z16110000916005)+3 种基金National Science and Technology Major Project (2016ZX08004-003)National Key R&D Program of China (2016YFD0100504 and 2016YFD0100201)National Natural Science Foundation of China (31671716, 31171576)Agricultural Science and Technology Innovation Project of CAAS
文摘Soybean isoflavones are essential secondary metabolites synthesized in the phenylpropanoid pathway and benefit human health. In the present study, highresolution QTL mapping for isoflavone components was performed using specific-locus amplified fragment sequencing(SLAF-seq) with a recombinant inbred line(RIL) population(F5:7) derived from a cross between two cultivated soybean varieties, Luheidou 2(LHD2) and Nanhuizao(NHZ). Using a high-density genetic map comprising 3541 SLAF markers and the isoflavone contents of soybean seeds in the 200 lines in four environments, 24 stable QTL were identified for isoflavone components, explaining 4.2%–21.2% of phenotypic variation.Of these QTL, four novel stable QTL(qG8, qMD19, qMG18, and qTIF19) were identified for genistin, malonyldaidzin, malonylgenistin, and total isoflavones, respectively. Gene annotation revealed three genes involved in isoflavone biosynthesis(Gm4CL, GmIFR, and GmCHR) and 13 MYB-like genes within genomic regions corresponding to stable QTL intervals, suggesting candidate genes underlying these loci. Nine epistatic QTL were identified for isoflavone components, explaining 4.7%–15.6% of phenotypic variation. These results will facilitate understanding the genetic basis of isoflavone accumulation in soybean seeds. The stable QTL and tightly linked SLAF markers may be used for markerassisted selection in soybean breeding programs.
文摘特异长度扩增片段测序(specific length amplified fragment sequencing,SLAF-seq)技术是以第二代测序为基础的一种简化基因组测序技术。本研究简述了SLAF-seq技术流程,综述了该技术在蔬菜分子标记开发、高密度遗传图谱构建、基因定位、遗传多样性分析和辅助全基因组测序等方面的应用,讨论了其应用存在的问题,展望了其发展前景。
基金supported by the National Natural Science Foundation of China(No.31470409)
文摘To effectively use elite genes on the long arm of rye chromosome 6（the 6RL arm） in wheat breeding programs,precise and fast identification of 6RL chromatin in wheat backgrounds is necessary.PCR-based 6RL-specific markers can facilitate the detection of elite genes on 6RL in wheat breeding.However,only a limited number of 6RL-specific markers have been developed.In the present study.300 new PCR-based 6RL-specific markers were identified using specific length amplified fragment sequencing（SLAF-seq） technology,and were further physically mapped to four regions on the 6RL arm using 6R and 6RL deletion lines.Interestingly,127 of the 300 markers were physically localized to a region from the site between 2.3 and 2.5 to the telomere,the same region where the powdery mildew resistance gene was mapped.In addition,95 of the 300 markers exhibit polymorphisms,which can be used to investigate the diversity of rye 6RL arms.The markers developed in this study can be used to identify given segments of 6RL in wheat backgrounds and accelerate the utilization of elite genes on 6RL in wheat breeding.
