酒药是传统黄酒酿造过程中重要的动力源,具有糖化和发酵的双重作用,绍兴酒药赋予绍兴酒独特的品质。为探究绍兴地区不同酒厂酒药微生物组成、理化指标及其特性,首先通过单分子实时定量测序技术(single molecule real time sequencing,SM...酒药是传统黄酒酿造过程中重要的动力源,具有糖化和发酵的双重作用,绍兴酒药赋予绍兴酒独特的品质。为探究绍兴地区不同酒厂酒药微生物组成、理化指标及其特性,首先通过单分子实时定量测序技术(single molecule real time sequencing,SMRT-seq)研究酒药的微生物组成,然后测定酒药水分含量、酸度及酶活性等相关理化指标,最后筛选鉴定酒药中核心微生物并研究其功能特性。微生物群落结构分析结果表明,不同酒厂酒药细菌群落种类及相对丰度差异较大,以戊糖片球菌(Pediococcus pentosaceus)、食窦魏斯氏菌(Weissella cibaria)、肠杆菌属为主(Enterococcus);真菌较为稳定,以扣囊复膜酵母(Saccharomycopsis fibuligera)为主。理化指标结果显示,JH酒厂酒药水分含量(14.52%)显著高于其他酒厂样品(P<0.05),TP和JH酒厂的酒药酸度(33.14和35.49 g/kg)和液化力(1.4和1.31 U/g)显著高于其他酒厂样品(P<0.05),SYH酒厂酒药的酸性蛋白酶活力最高,为190.08 U/g(P<0.05)。不同厂区酒药存在一定差异,但在核心功能性微生物组成及特性方面具有一致性,筛选得到71株微生物,功能分析结果显示扣囊复膜酵母对酒药酵母的淀粉酶和蛋白酶活性贡献最大,异常威克汉姆酵母可能是潜在的风味贡献者。该研究为未来酒药机械化生产提供理论依据,筛选获得的功能微生物能够为机械化黄酒风味提升提供菌种。展开更多
This study was designed to generate the full-length transcriptome of Coilia nasus using single-molecule long read isoform sequencing(SMRT-seq)technology.RNAs of brains,ovaries,and testes of C.nasus conditioned in both...This study was designed to generate the full-length transcriptome of Coilia nasus using single-molecule long read isoform sequencing(SMRT-seq)technology.RNAs of brains,ovaries,and testes of C.nasus conditioned in both seawater and freshwater except for gut which was only from the fish conditioned in freshwater,were isolated.All the RNAs were pooled together equally for SMRT sequencing.One SMRT cell produced 64.18 Gb clean data,including 765,184 circular consensus(CCS)reads and 616,126 full-length non-chimeric(FLNC)reads.Finally,93,793 non-redundant transcripts were obtained after clustered and polished.Structural analysis of the 93,793 non-redundant transcripts predicted 8242 alternative splicing events,a total of 229,825 SSR and 72,037 complete CDS.A total of 8437 lncRNA were identified.84,617 transcripts were annotated in NR,Swissprot,GO,COG,KOG,eggnog,Pfam and KEGG database totally.This is the first full-length transcriptome of Coilia nasus,including transcripts appeared in the case of seawater,which will facilitate exploration of genetic data and life story of Coilia nasus.展开更多
基金This work was supported by the Project of Centre for Research on Environmental Ecology and Fish Nutrition(CREEFN)of the Ministry of Agriculture and Rural Affairs,Shanghai Ocean University(A1-3201-19-300204)the Project of Science and Technology Commission Shanghai Municipality(17391900300).
文摘This study was designed to generate the full-length transcriptome of Coilia nasus using single-molecule long read isoform sequencing(SMRT-seq)technology.RNAs of brains,ovaries,and testes of C.nasus conditioned in both seawater and freshwater except for gut which was only from the fish conditioned in freshwater,were isolated.All the RNAs were pooled together equally for SMRT sequencing.One SMRT cell produced 64.18 Gb clean data,including 765,184 circular consensus(CCS)reads and 616,126 full-length non-chimeric(FLNC)reads.Finally,93,793 non-redundant transcripts were obtained after clustered and polished.Structural analysis of the 93,793 non-redundant transcripts predicted 8242 alternative splicing events,a total of 229,825 SSR and 72,037 complete CDS.A total of 8437 lncRNA were identified.84,617 transcripts were annotated in NR,Swissprot,GO,COG,KOG,eggnog,Pfam and KEGG database totally.This is the first full-length transcriptome of Coilia nasus,including transcripts appeared in the case of seawater,which will facilitate exploration of genetic data and life story of Coilia nasus.
