筛选高效脱硫菌株并揭示其去除硫化物的机理对生物脱硫技术的发展具有重要意义.从天然气净化厂污水站废水中分离出一株化能自养型硫氧化细菌,命名为DS-B.根据16S r DNA基因序列相似性分析,结合形态特征,对该菌株进行鉴定.进一步研究DS-...筛选高效脱硫菌株并揭示其去除硫化物的机理对生物脱硫技术的发展具有重要意义.从天然气净化厂污水站废水中分离出一株化能自养型硫氧化细菌,命名为DS-B.根据16S r DNA基因序列相似性分析,结合形态特征,对该菌株进行鉴定.进一步研究DS-B的硫代硫酸盐耐受性,并通过测定培养体系中各价态含硫物质的含量,探讨该菌的硫氧化性能.结果显示,菌株DS-B与Thioalkalivibrio thiocyanoxidans ARh2的序列相似性最高,初步鉴定其属于Thioalkalivibrio.DS-B的最佳生长和降解温度为35℃,35℃下培养7 d,硫代硫酸盐(S2O32-)的去除率能达到98.7%.该菌对S2O32-有较强的耐受性,且最适S2O32-浓度为2×104 mg/L.此外,对各价态硫氧化物的分析表明该菌氧化S2O32-的途径为:S2O32-→SO42-/S2O32-→S→SO42-.上述表明,菌株DS-B具有较强的耐盐能力,对硫代硫酸盐具有较高的去除能力,是一株具有应用价值的硫氧化细菌.展开更多
Sulfur-driven autotrophic denitrification(SDAD),a process suited for the treatment of nitrogen and sulfur-polluted wastewater without extra supplement of organic carbon,is a promising biological nitrogen removal proce...Sulfur-driven autotrophic denitrification(SDAD),a process suited for the treatment of nitrogen and sulfur-polluted wastewater without extra supplement of organic carbon,is a promising biological nitrogen removal process.However,the SDAD process was affected by many factors such as various electron donors,organic carbon and exogenous substances(e.g.,antibiotics and heavy metal),which prevent further application.Thus,we conducted a detailed review of previous studies on such influence factors and its current application.Besides,a comparative analysis was adopted to recognize the current challenges and future needs for feasible application,so as to ultimately perfect the SDAD process and extend its application scope.展开更多
Sulfur-oxidizing bacteria （SOB） are the main microorganisms that participate in the natural sulfur cycle. To obtain SOBwith high sulfur-oxidizing ability under aerobic or anaerobic conditions, aerobic and anaerobic ...Sulfur-oxidizing bacteria （SOB） are the main microorganisms that participate in the natural sulfur cycle. To obtain SOBwith high sulfur-oxidizing ability under aerobic or anaerobic conditions, aerobic and anaerobic enrichmentswere carried out. Denaturing gradient gel electrophoresis （DGGE） profiles showed that the microbial community changed according to the thiosulfate utilizationduring enrichments, and Rhodopseudomonas and Halothiobacilluswere the predominant bacteria in anaerobic enrichment and aerobic enrichment, respectively,which mainly contributed to the thiosulfate oxidization in the enrichments. Based on the enriched cultures, six isolateswere isolated from the aerobic enrichment and four isolateswere obtained from the anaerobic enrichment. Phylogenetic analysis suggested the 16S rRNA gene of isolates belonged to the genus Acinetobacter, Rhodopseudomonas, Pseudomonas, Halothiobacillus,0chrobactrum, Paracoccus, Thiobacillus, and Alcaligenes, respectively. The tests suggested isolates related to Halothiobacillus and Rhodopseudomonas had the highest thiosulfate oxidizing ability under aerobic or anaerobic conditions, respectively; Paracoccus and Alcaligenes could aerobically and anaerobically oxidize thiosulfate. Based on the DGGE and thiosulfate oxidizing ability analysis, Rhodopseudomonas and Halothiobacilluswere found to be the main SOB in the sulfide-removing reactor, andwere responsible for the sulfur-oxidizing in the treatment system.展开更多
The removal of ciprofloxacin(CIP)in sulfur-mediated bio processes,e.g.,sulfate-reducing bacteria(SRB)-mediated process and sulfur-oxidizing bacteria(SOB)-mediated process,was examined for the first time.The results sh...The removal of ciprofloxacin(CIP)in sulfur-mediated bio processes,e.g.,sulfate-reducing bacteria(SRB)-mediated process and sulfur-oxidizing bacteria(SOB)-mediated process,was examined for the first time.The results showed that the SRB-mediated process had more efficient CIP removal than that in SOB-mediated process.Adsorption was the primary removal pathway of CIP in SRB-mediated process and SOB-mediated process with the specific adsorption removal rate of 131.4±1.1μg/g-SS/d and30.1±1.4μg/g-SS/d,respectively,at influent CIP concentration of 500μg/L.In addition,extracellular polymeric substances(EPS)also played an important role on CIP migration and removal in both types of sludge.Further study was conducted to specify the different adsorption of CIP in these two sludge systems from the perspective of sludge properties.The results indicated that there are more potential adsorption sites exist on the SRB-mediated sludge for CIP adsorption than SOB-mediated sludge since the higher protein(PN)content and more kinds of aromatic amino acid substances in EPS,more negative zeta-potential and stronger and more numbers of functional groups in SRB-mediated sludge compared to SOB-mediated sludge.The findings of this study provide insights into the sludge properties affecting CIP removal in sulfur-mediated bioprocesses,and are of guiding significance to employ sulfur-mediated biological systems for treating CIP-containing wastewaters.展开更多
基金the financial support from the National Natural Science Foundation of China(No.51878231)。
文摘Sulfur-driven autotrophic denitrification(SDAD),a process suited for the treatment of nitrogen and sulfur-polluted wastewater without extra supplement of organic carbon,is a promising biological nitrogen removal process.However,the SDAD process was affected by many factors such as various electron donors,organic carbon and exogenous substances(e.g.,antibiotics and heavy metal),which prevent further application.Thus,we conducted a detailed review of previous studies on such influence factors and its current application.Besides,a comparative analysis was adopted to recognize the current challenges and future needs for feasible application,so as to ultimately perfect the SDAD process and extend its application scope.
基金supported by the National Natural Science Foundation of China(No.21076090,21276099)the Specialized Research Found for the Doctoral Program of Higher Education of China(No.20120172120045)
文摘Sulfur-oxidizing bacteria （SOB） are the main microorganisms that participate in the natural sulfur cycle. To obtain SOBwith high sulfur-oxidizing ability under aerobic or anaerobic conditions, aerobic and anaerobic enrichmentswere carried out. Denaturing gradient gel electrophoresis （DGGE） profiles showed that the microbial community changed according to the thiosulfate utilizationduring enrichments, and Rhodopseudomonas and Halothiobacilluswere the predominant bacteria in anaerobic enrichment and aerobic enrichment, respectively,which mainly contributed to the thiosulfate oxidization in the enrichments. Based on the enriched cultures, six isolateswere isolated from the aerobic enrichment and four isolateswere obtained from the anaerobic enrichment. Phylogenetic analysis suggested the 16S rRNA gene of isolates belonged to the genus Acinetobacter, Rhodopseudomonas, Pseudomonas, Halothiobacillus,0chrobactrum, Paracoccus, Thiobacillus, and Alcaligenes, respectively. The tests suggested isolates related to Halothiobacillus and Rhodopseudomonas had the highest thiosulfate oxidizing ability under aerobic or anaerobic conditions, respectively; Paracoccus and Alcaligenes could aerobically and anaerobically oxidize thiosulfate. Based on the DGGE and thiosulfate oxidizing ability analysis, Rhodopseudomonas and Halothiobacilluswere found to be the main SOB in the sulfide-removing reactor, andwere responsible for the sulfur-oxidizing in the treatment system.
基金supported by the National Natural Science Foundation of China(Nos.51922110 and 51778643)。
文摘The removal of ciprofloxacin(CIP)in sulfur-mediated bio processes,e.g.,sulfate-reducing bacteria(SRB)-mediated process and sulfur-oxidizing bacteria(SOB)-mediated process,was examined for the first time.The results showed that the SRB-mediated process had more efficient CIP removal than that in SOB-mediated process.Adsorption was the primary removal pathway of CIP in SRB-mediated process and SOB-mediated process with the specific adsorption removal rate of 131.4±1.1μg/g-SS/d and30.1±1.4μg/g-SS/d,respectively,at influent CIP concentration of 500μg/L.In addition,extracellular polymeric substances(EPS)also played an important role on CIP migration and removal in both types of sludge.Further study was conducted to specify the different adsorption of CIP in these two sludge systems from the perspective of sludge properties.The results indicated that there are more potential adsorption sites exist on the SRB-mediated sludge for CIP adsorption than SOB-mediated sludge since the higher protein(PN)content and more kinds of aromatic amino acid substances in EPS,more negative zeta-potential and stronger and more numbers of functional groups in SRB-mediated sludge compared to SOB-mediated sludge.The findings of this study provide insights into the sludge properties affecting CIP removal in sulfur-mediated bioprocesses,and are of guiding significance to employ sulfur-mediated biological systems for treating CIP-containing wastewaters.
